##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937929_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2282055 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.128940801163864 33.0 31.0 34.0 31.0 34.0 2 32.317423112063466 34.0 31.0 34.0 31.0 34.0 3 32.3955702207002 34.0 31.0 34.0 31.0 34.0 4 35.91005606788618 37.0 35.0 37.0 35.0 37.0 5 35.92993288943518 37.0 35.0 37.0 35.0 37.0 6 35.96186025314903 37.0 37.0 37.0 35.0 37.0 7 35.90960384390385 37.0 37.0 37.0 35.0 37.0 8 35.89504240695338 37.0 36.0 37.0 35.0 37.0 9 37.60202142367296 39.0 38.0 39.0 35.0 39.0 10-11 37.531584471014064 39.0 38.0 39.0 35.0 39.0 12-13 37.44452171398147 39.0 37.5 39.0 35.0 39.0 14-15 38.76363409295569 40.0 38.0 41.0 35.0 41.0 16-17 38.68250151727281 40.0 38.0 41.0 35.0 41.0 18-19 38.61491002627018 40.0 38.0 41.0 34.5 41.0 20-21 38.501646323160486 40.0 38.0 41.0 34.0 41.0 22-23 38.36462114190938 40.0 38.0 41.0 34.0 41.0 24-25 38.31470450975108 40.0 38.0 41.0 34.0 41.0 26-27 38.33085486546118 40.0 38.0 41.0 34.0 41.0 28-29 38.25224413960225 40.0 38.0 41.0 34.0 41.0 30-31 38.0726051738455 40.0 38.0 41.0 34.0 41.0 32-33 37.860494817171364 40.0 38.0 41.0 33.0 41.0 34-35 37.66186003404826 40.0 37.0 41.0 33.0 41.0 36-37 37.44918395919467 40.0 37.0 41.0 32.5 41.0 38-39 37.206519343311186 40.0 37.0 41.0 31.5 41.0 40-41 36.98014399302383 40.0 36.0 41.0 31.0 41.0 42-43 36.68924675347439 39.0 36.0 41.0 30.5 41.0 44-45 36.486610313949484 39.0 35.5 41.0 30.0 41.0 46-47 36.3719629894985 39.0 36.0 41.0 30.0 41.0 48-49 36.11903876111663 39.0 35.0 41.0 29.5 41.0 50-51 35.46830159658729 38.5 34.5 40.5 28.5 40.5 52-53 35.53229457659872 38.5 35.0 40.0 28.0 41.0 54-55 35.65836603412275 39.0 35.0 40.5 28.0 41.0 56-57 35.43423887680183 39.0 35.0 40.5 28.0 41.0 58-59 35.16117118123796 38.0 35.0 40.0 27.5 41.0 60-61 34.77518683817875 38.0 34.5 40.0 26.0 41.0 62-63 34.35088527664758 37.0 34.0 40.0 26.0 41.0 64-65 33.919989001141516 36.5 34.0 39.5 25.5 41.0 66-67 33.48905942231892 36.0 33.5 39.0 23.5 41.0 68-69 33.0044448972527 35.5 33.0 39.0 22.5 40.5 70-71 32.52214495268519 35.0 32.5 38.0 21.5 40.0 72-73 32.04561042569088 35.0 32.0 37.0 19.5 39.5 74-75 31.53846993170629 35.0 31.5 37.0 18.0 39.0 76-77 31.028930284327064 35.0 31.0 36.0 15.0 39.0 78-79 30.487035369436757 34.5 30.5 36.0 8.5 38.0 80-81 29.982361511882928 34.0 30.0 35.0 6.5 37.0 82-83 29.467589738196494 34.0 29.0 35.0 2.0 37.0 84-85 29.00709798843586 34.0 29.0 35.0 2.0 36.0 86-87 28.542683896750955 34.0 29.0 35.0 2.0 36.0 88-89 28.005691361514074 33.5 27.5 35.0 2.0 35.0 90-91 27.418078004254937 33.0 26.5 35.0 2.0 35.0 92-93 26.83008275435956 33.0 25.0 35.0 2.0 35.0 94-95 26.24644322770485 32.5 24.5 35.0 2.0 35.0 96-97 25.4271478995905 32.0 21.5 34.5 2.0 35.0 98-99 24.360444862196573 32.0 9.5 34.0 2.0 35.0 100-101 21.42753176413364 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3992004706400891 1101 2 0.22866943702334908 1101 3 0.3086260145286488 1101 4 0.22151681323362737 1101 5 0.23462929792801646 1101 6 0.2606395616116757 1101 7 0.29353895962322696 1101 8 0.36593562085109 1101 9 0.3111706644718666 1101 10-11 0.40683992309445216 1101 12-13 0.3119974661551126 1101 14-15 0.4058700570554734 1101 16-17 0.4621573966271413 1101 18-19 0.40693508549280466 1101 20-21 0.4303771333404214 1101 22-23 0.4211945267688435 1101 24-25 0.37659328053125307 1101 26-27 0.36156383548977544 1101 28-29 0.39984121494178737 1101 30-31 0.37894921220932076 1101 32-33 0.35569091496981287 1101 34-35 0.4484584366612765 1101 36-37 0.48608907772169374 1101 38-39 0.5229085595001948 1101 40-41 0.6354694511443668 1101 42-43 0.5664616879210556 1101 44-45 0.6407963327613473 1101 46-47 0.7746542852643685 1101 48-49 0.8398260940180577 1101 50-51 0.778195549956223 1101 52-53 0.8631521339611865 1101 54-55 0.8517349259172846 1101 56-57 0.7010500413492977 1101 58-59 0.6802695952228461 1101 60-61 0.7960671264370092 1101 62-63 0.9276064874368473 1101 64-65 0.9915750192522879 1101 66-67 0.8159142376397739 1101 68-69 0.8776070454761964 1101 70-71 0.9263561601457582 1101 72-73 0.8415930706441657 1101 74-75 0.8980577276520272 1101 76-77 0.9918063547411968 1101 78-79 0.8912577261502719 1101 80-81 1.2236561004794275 1101 82-83 0.9833240232945286 1101 84-85 1.1280706036034438 1101 86-87 1.1214142111758107 1101 88-89 1.245811163992709 1101 90-91 1.350302733267295 1101 92-93 1.3749255677480292 1101 94-95 1.2773293218635295 1101 96-97 1.4999707230087687 1101 98-99 1.6376172321264484 1101 100-101 1.5778796658243799 1102 1 0.37931135075096734 1102 2 0.25130580965972626 1102 3 0.1840170649197006 1102 4 0.15718654890336126 1102 5 0.12881799211670852 1102 6 0.2267097526818631 1102 7 0.16004722613149625 1102 8 0.16894769886316396 1102 9 0.3373413406425385 1102 10-11 0.30128478003930326 1102 12-13 0.15878367544132033 1102 14-15 0.22561933930475675 1102 16-17 0.25387727584702446 1102 18-19 0.2369061279638487 1102 20-21 0.28105404651733323 1102 22-23 0.22572130629562537 1102 24-25 0.2270499184878858 1102 26-27 0.17830970223564435 1102 28-29 0.2215271866277604 1102 30-31 0.20108810934821975 1102 32-33 0.19256845184735027 1102 34-35 0.19291443111728057 1102 36-37 0.1834358750684899 1102 38-39 0.2527521393437837 1102 40-41 0.29665522483014684 1102 42-43 0.29821968217904526 1102 44-45 0.2056438726088814 1102 46-47 0.35988470799478733 1102 48-49 0.23342078761275076 1102 50-51 0.30891899317966676 1102 52-53 0.2747697955788482 1102 54-55 0.2853367970191556 1102 56-57 0.2826917104909725 1102 58-59 0.2708497358029902 1102 60-61 0.2685017863716723 1102 62-63 0.2892785991089539 1102 64-65 0.17557412825139807 1102 66-67 0.10642384064937005 1102 68-69 0.214845932715086 1102 70-71 0.383622767412362 1102 72-73 0.4824615490126405 1102 74-75 0.41520578230008454 1102 76-77 0.37006098299582746 1102 78-79 0.5044160768086208 1102 80-81 0.617744557067887 1102 82-83 0.5388841163546196 1102 84-85 0.5295667425995809 1102 86-87 0.38864022840182955 1102 88-89 0.3917175098990562 1102 90-91 0.5749005453651073 1102 92-93 0.4081797010021617 1102 94-95 0.4060121255463365 1102 96-97 0.5524655255035711 1102 98-99 0.6243871063963198 1102 100-101 0.638534551479264 1103 1 0.22416673244845242 1103 2 0.18145042796223976 1103 3 0.18215599332178556 1103 4 0.10229086374452123 1103 5 9.730419033360249E-4 1103 6 0.10090368345473877 1103 7 0.10994054049849211 1103 8 0.15021844329180567 1103 9 0.13321616283315052 1103 10-11 0.226261072120856 1103 12-13 0.04173768207953543 1103 14-15 0.22147638055653118 1103 16-17 0.28459088419220535 1103 18-19 0.24133358173340724 1103 20-21 0.1954388260336941 1103 22-23 0.18287517068633008 1103 24-25 0.2624280597870836 1103 26-27 0.20928409768372092 1103 28-29 0.39116327007963037 1103 30-31 0.3530262316810777 1103 32-33 0.46167703411383343 1103 34-35 0.471855872558713 1103 36-37 0.43110064510168655 1103 38-39 0.36055797149171553 1103 40-41 0.39916233720568073 1103 42-43 0.3916110999125735 1103 44-45 0.3156782490906309 1103 46-47 0.4710274296638275 1103 48-49 0.5019959417142275 1103 50-51 0.3942260107235285 1103 52-53 0.5987266471672825 1103 54-55 0.6350345210589836 1103 56-57 0.498338936664517 1103 58-59 0.48527007719700777 1103 60-61 0.5047571206533163 1103 62-63 0.43905337388373056 1103 64-65 0.35287972332015016 1103 66-67 0.4275298150448208 1103 68-69 0.5136249749151816 1103 70-71 0.501071795387702 1103 72-73 0.5009240905014991 1103 74-75 0.43337197480838086 1103 76-77 0.4630493661157864 1103 78-79 0.6132887652865726 1103 80-81 0.5410553678786982 1103 82-83 0.49893034679558923 1103 84-85 0.5933588241548229 1103 86-87 0.5766677295872249 1103 88-89 0.6254598048255566 1103 90-91 0.7078841869013814 1103 92-93 0.7320298669575926 1103 94-95 0.8925934437065983 1103 96-97 0.7557905610654494 1103 98-99 0.7404729290610916 1103 100-101 0.7285712987528576 1104 1 0.3587620918146115 1104 2 0.30680130515521853 1104 3 0.2473823657043539 1104 4 0.18674191071679047 1104 5 0.24791418508387153 1104 6 0.25349847688994487 1104 7 0.15212220433550527 1104 8 0.23282738500091682 1104 9 0.22907920754169453 1104 10-11 0.30296452429969634 1104 12-13 0.21727248683335887 1104 14-15 0.3397998100981354 1104 16-17 0.43502665102865734 1104 18-19 0.5081063604543985 1104 20-21 0.5257939477088485 1104 22-23 0.482729443465054 1104 24-25 0.5633010082873682 1104 26-27 0.49135823463900863 1104 28-29 0.5441499707021578 1104 30-31 0.5148442848785919 1104 32-33 0.6374580188336907 1104 34-35 0.580108560730757 1104 36-37 0.6840519750394307 1104 38-39 0.6627608577395918 1104 40-41 0.6393986133799885 1104 42-43 0.6973086098163606 1104 44-45 0.5905523294528265 1104 46-47 0.6276629701278935 1104 48-49 0.5755132026083984 1104 50-51 0.5610157673732203 1104 52-53 0.45835596239082577 1104 54-55 0.5768610632208393 1104 56-57 0.47583469218233887 1104 58-59 0.4290377625394015 1104 60-61 0.48141954283781985 1104 62-63 0.49426842764717094 1104 64-65 0.5092620096812155 1104 66-67 0.6669454913323136 1104 68-69 0.7005463105122303 1104 70-71 0.6428686261743479 1104 72-73 0.7138768352666389 1104 74-75 0.74691894707777 1104 76-77 0.6036195415543837 1104 78-79 0.5996427550998149 1104 80-81 0.7118887012120325 1104 82-83 0.7077376473049277 1104 84-85 0.7182894976126626 1104 86-87 0.7625024777479474 1104 88-89 0.7698924590417455 1104 90-91 0.6016122570768196 1104 92-93 0.8811526739751372 1104 94-95 1.0108333984321227 1104 96-97 1.0030104252420209 1104 98-99 1.114465571958661 1104 100-101 0.9483180446498558 1105 1 0.35312658498897065 1105 2 0.2761999377769584 1105 3 0.2023224360086644 1105 4 0.16692977894797423 1105 5 0.0627567655489969 1105 6 0.1068507140702053 1105 7 0.08304772767950652 1105 8 0.16081489045827624 1105 9 0.26958277001666175 1105 10-11 0.25922300290009304 1105 12-13 0.10715703833958656 1105 14-15 0.24845759195674333 1105 16-17 0.29591765234741985 1105 18-19 0.2824097311903486 1105 20-21 0.33301611742038517 1105 22-23 0.2522911892803279 1105 24-25 0.37850384206404186 1105 26-27 0.17974722179790348 1105 28-29 0.40758254923188275 1105 30-31 0.43896865418774667 1105 32-33 0.23515544025894997 1105 34-35 0.2279968660008862 1105 36-37 0.2910345159865102 1105 38-39 0.2629184798156814 1105 40-41 0.2485356527675009 1105 42-43 0.3486353448069295 1105 44-45 0.19877172209923089 1105 46-47 0.25570592686958804 1105 48-49 0.1969367523007648 1105 50-51 0.09126516312525723 1105 52-53 0.18863929504902188 1105 54-55 0.22624322249025397 1105 56-57 0.27385041838261515 1105 58-59 0.27709674254182914 1105 60-61 0.3099445476763023 1105 62-63 0.2407312028219195 1105 64-65 0.2030883733462261 1105 66-67 0.31987824314731483 1105 68-69 0.4350569034178946 1105 70-71 0.5648210334892383 1105 72-73 0.5666145173849486 1105 74-75 0.5802594818765918 1105 76-77 0.4547985564839223 1105 78-79 0.5718698533143005 1105 80-81 0.6776484333175148 1105 82-83 0.5685688764600592 1105 84-85 0.6348364849245804 1105 86-87 0.6132583165611472 1105 88-89 0.6077010530906328 1105 90-91 0.36906307331792476 1105 92-93 0.6371454184986476 1105 94-95 0.7144432230314273 1105 96-97 0.7234589865242391 1105 98-99 0.897075743128493 1105 100-101 0.7630982424201029 1106 1 -0.02566291733805315 1106 2 -0.03613270072960262 1106 3 -0.1162749042247313 1106 4 -0.04879484232392883 1106 5 -0.055813865465971446 1106 6 -0.06810751098785772 1106 7 0.010301705074496681 1106 8 -0.1664084012470326 1106 9 -0.12313546655558127 1106 10-11 -0.5550175500335186 1106 12-13 -0.36042266835519143 1106 14-15 -0.23656298804150566 1106 16-17 -0.13731792313506475 1106 18-19 -0.09505029251716479 1106 20-21 -0.11172113724145305 1106 22-23 -0.25014096833714206 1106 24-25 -0.11715784539200769 1106 26-27 -0.18865234177557966 1106 28-29 -0.19894729163360836 1106 30-31 -0.21082440215445786 1106 32-33 -0.06689810802905072 1106 34-35 -0.27563782227104383 1106 36-37 -0.3813221995256484 1106 38-39 -0.13890913100601665 1106 40-41 0.052297500144554476 1106 42-43 -0.17020362763770436 1106 44-45 -0.16432136374979933 1106 46-47 0.010704096109265038 1106 48-49 0.15514465523826715 1106 50-51 0.14089671491149147 1106 52-53 0.15803894114308292 1106 54-55 -0.0865532203626671 1106 56-57 0.0760988313571076 1106 58-59 0.25751543681295175 1106 60-61 0.10392832261788243 1106 62-63 0.14465446677991167 1106 64-65 0.1703641263200879 1106 66-67 0.05805154022789338 1106 68-69 -0.27830330100791656 1106 70-71 -0.044917784488845314 1106 72-73 0.06937860478215896 1106 74-75 0.14908982397101056 1106 76-77 0.22963887740978706 1106 78-79 0.38067029445628364 1106 80-81 0.28532291726919823 1106 82-83 0.37625247462461786 1106 84-85 0.2612110287520686 1106 86-87 0.2705915365826428 1106 88-89 -0.003933626735690865 1106 90-91 0.016004859010404715 1106 92-93 0.06396057645516962 1106 94-95 -0.11026600630556871 1106 96-97 0.07043100043625827 1106 98-99 -0.019437803002357867 1106 100-101 -0.1890392076518701 1107 1 0.255612278544433 1107 2 0.16258724034413774 1107 3 0.1508854258179113 1107 4 0.10919676807775858 1107 5 0.0590116185789924 1107 6 -0.02423821020639849 1107 7 -0.09744235299987736 1107 8 -0.022655097963507842 1107 9 0.0019305567242966504 1107 10-11 0.03905538646662876 1107 12-13 -0.06353715284965489 1107 14-15 0.06462364211503058 1107 16-17 0.03492810279337277 1107 18-19 0.05723690650358293 1107 20-21 0.05942570175466244 1107 22-23 0.13574214803288953 1107 24-25 0.20146164364588515 1107 26-27 0.120773061116914 1107 28-29 0.13203209452072429 1107 30-31 0.14962582616951892 1107 32-33 0.161645364580977 1107 34-35 0.24668275167663722 1107 36-37 0.20101016577711306 1107 38-39 0.21351588816722966 1107 40-41 0.13766638524429453 1107 42-43 0.4544688417864151 1107 44-45 0.4358374990711624 1107 46-47 0.40041516382375875 1107 48-49 0.3198938278171397 1107 50-51 0.3404942251280403 1107 52-53 0.2885522347344178 1107 54-55 0.2915052789339043 1107 56-57 0.32026398425727365 1107 58-59 0.2598540386132626 1107 60-61 0.3593650376826858 1107 62-63 0.46182801419568875 1107 64-65 0.4332855710374659 1107 66-67 0.3656513517425566 1107 68-69 0.3792906012642234 1107 70-71 0.5475228615363363 1107 72-73 0.5461700336166473 1107 74-75 0.5067514324278228 1107 76-77 0.5330131142315402 1107 78-79 0.5318431307282125 1107 80-81 0.5713884995177985 1107 82-83 0.5615848021150107 1107 84-85 0.37936840993856435 1107 86-87 0.4820918890176671 1107 88-89 0.43231183556800445 1107 90-91 0.5577800666774664 1107 92-93 0.6591142176083196 1107 94-95 0.8366091404717082 1107 96-97 0.9005393455923176 1107 98-99 0.594724608077108 1107 100-101 0.5661296839251442 1108 1 0.23899183869232132 1108 2 0.133550787556878 1108 3 0.12126735869173189 1108 4 0.09320020230831716 1108 5 0.14110325874980134 1108 6 0.24100665828311918 1108 7 0.16344658822650615 1108 8 0.24222563518458173 1108 9 0.22830715117356704 1108 10-11 0.2641519950804323 1108 12-13 0.1387791989151097 1108 14-15 0.1476900124623839 1108 16-17 0.19402751925813533 1108 18-19 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-0.6308440808774591 2316 50-51 -0.7552158156734521 2316 52-53 -0.7407920439266533 2316 54-55 -0.8222596827604249 2316 56-57 -0.6199806298574302 2316 58-59 -0.7295005641951988 2316 60-61 -0.6924776034809597 2316 62-63 -0.7387299929841404 2316 64-65 -0.6647307641661726 2316 66-67 -0.7418509431887941 2316 68-69 -0.6463894978865454 2316 70-71 -0.7311310709330279 2316 72-73 -0.7246640876904635 2316 74-75 -0.7627887615395288 2316 76-77 -0.7776011157226108 2316 78-79 -0.8307892995939348 2316 80-81 -0.6776485221280097 2316 82-83 -0.836703191274939 2316 84-85 -0.785603105316742 2316 86-87 -0.9069585897322021 2316 88-89 -0.7601631433900486 2316 90-91 -0.6542831151565807 2316 92-93 -0.9117380034225846 2316 94-95 -0.9984987233307088 2316 96-97 -1.0259114933664044 2316 98-99 -1.066061893911833 2316 100-101 -1.2482514800250755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5088.0 3 1244.0 4 1167.0 5 1310.0 6 1563.0 7 1906.0 8 2258.0 9 3043.0 10 3817.0 11 4795.0 12 6396.0 13 8334.0 14 9789.0 15 12377.0 16 15229.0 17 20373.0 18 18513.0 19 15037.0 20 14404.0 21 14574.0 22 15716.0 23 17187.0 24 19735.0 25 22025.0 26 25030.0 27 29941.0 28 35458.0 29 42932.0 30 52363.0 31 65576.0 32 83632.0 33 111425.0 34 156734.0 35 233977.0 36 356451.0 37 475749.0 38 337400.0 39 39503.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68142531954333 17.631702924872645 13.578753175620045 24.108118579963982 2 15.984611674959995 18.931892413248868 39.712181327955456 25.37131458383568 3 19.800189936188104 22.47280080464195 29.082203084402348 28.644806174767602 4 13.062134932115626 15.32222879465607 35.51258479791666 36.103051475311645 5 14.097208213098506 36.67923833247086 33.96253058861709 15.261022865813548 6 34.36206906383806 34.707690930968184 16.21681137398181 14.71342863121195 7 30.029875040506486 32.07499762113467 19.38659039096268 18.508536947396163 8 27.373198508651015 34.33579377856344 18.859628355272157 19.43137935751339 9 26.466503224991843 16.323737272768724 19.587002879519634 37.62275662271979 10-11 25.802945555246154 25.67934800483252 26.36550407058457 22.152202369336756 12-13 26.49204110947414 22.926754694476557 27.062794004528385 23.518410191520914 14-15 22.929807985303498 24.547782262042297 25.190447274329568 27.331962478324634 16-17 22.83388612620339 27.771116786290474 26.270826194599312 23.12417089290682 18-19 23.039964397168873 25.978294111392575 28.484742191876023 22.49699929956253 20-21 23.054539634092862 25.293287024855726 29.05821887772106 22.593954463330352 22-23 22.802156543899645 25.258446978823713 28.903221902544228 23.03617457473241 24-25 22.726343720532444 25.50117321988553 28.95673340496919 22.815749654612837 26-27 22.165116052176067 25.946225632222088 28.75147305636037 23.137185259241477 28-29 22.39029667135416 26.435615822062964 28.530911798405374 22.643175708177505 30-31 22.474841987487924 25.847770810544386 28.515374139994687 23.162013061973003 32-33 22.037730818588578 25.954161986473245 28.546837490163647 23.46126970477453 34-35 22.996380685970404 26.031368557639667 28.38682786321462 22.585422893175313 36-37 23.118894295353147 25.523125296398753 28.336586639643357 23.021393768604742 38-39 22.52282800510009 25.896412508775786 28.35619732199044 23.22456216413368 40-41 23.02389438938107 25.7637627443575 28.401485538402454 22.810857327858976 42-43 22.676739635485426 25.765528167802344 28.266899771753707 23.290832424958523 44-45 22.600574076614553 26.11079967934459 28.578456918175455 22.710169325865397 46-47 23.17624201567224 26.375780899855243 27.731689329793145 22.716287754679374 48-49 22.81699820967433 26.519481105807174 27.575595728728736 23.08792495578976 50-51 22.97014288181371 26.753565470622835 27.639004920520406 22.63728672704305 52-53 23.385737469413876 26.256192560270964 27.67258354545923 22.685486424855934 54-55 22.701175371204457 26.616288814512608 27.83362816963636 22.848907644646573 56-57 22.776660092044708 26.838023230330922 27.53819855358317 22.847118124041202 58-59 22.77273809069859 26.268180019171115 27.575905401442963 23.38317648868733 60-61 22.354616619013157 26.598175067140822 27.999486280128995 23.04772203371703 62-63 22.57612019255739 26.58474482113582 28.096487320003394 22.742647666303398 64-65 22.289600998621108 26.62142249159123 27.954181068450957 23.13479544133671 66-67 22.254006620085747 26.671451126740347 27.846499911797927 23.228042341375975 68-69 22.93648026552953 26.166342957960527 27.998323128273018 22.898853648236926 70-71 22.620476269697804 26.438835950793543 27.538776396806224 23.40191138270243 72-73 22.62220854522811 26.548251076296147 27.5992754700042 23.23026490847154 74-75 22.571848688018594 26.94945228450215 27.500751203404842 22.97794782407441 76-77 22.743077546816274 26.546914892451323 27.551549950142995 23.15845761058941 78-79 22.82964717009206 26.761656890001507 27.492753785157042 22.91594215474939 80-81 23.116147097714084 26.82128899615939 27.13351685412464 22.92904705200189 82-83 22.830210263200456 26.97424447825159 27.267354176007824 22.928191082540128 84-85 22.743167370362634 27.127222109851957 27.2443306765496 22.88527984323581 86-87 22.99525668817485 27.12588434487977 26.957555609069768 22.921303357875615 88-89 22.81771334506741 27.235506476028064 26.697192807871623 23.2495873710329 90-91 22.8778506941278 27.17620814714527 26.806696456756434 23.139244701970497 92-93 23.018949474351427 26.76571060857581 27.127876185136657 23.087463731936104 94-95 22.848801229209464 26.46342941811821 27.408332677500656 23.279436675171667 96-97 23.234990788263776 26.09419096186747 27.248611663669152 23.422206586199604 98-99 22.893648694438514 26.492726229452863 26.865411902708964 23.74821317339966 100-101 23.398658441625706 25.86180333467062 27.105896378178652 23.633641845525023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 646.0 1 465.0 2 449.5 3 635.0 4 523.0 5 451.5 6 873.0 7 1845.5 8 2995.0 9 3423.0 10 2862.0 11 2222.0 12 2064.0 13 2192.5 14 2314.0 15 2441.0 16 2625.5 17 2804.0 18 3008.5 19 3300.5 20 3801.0 21 4510.5 22 5409.5 23 6632.0 24 8115.0 25 10182.5 26 13099.5 27 16225.0 28 18691.0 29 21328.5 30 24317.5 31 28579.0 32 36458.0 33 39173.5 34 37658.5 35 39776.5 36 43351.0 37 47119.0 38 50328.5 39 53138.5 40 56352.0 41 61182.5 42 64060.0 43 66269.5 44 69294.0 45 74599.5 46 78531.5 47 80813.0 48 83589.5 49 83624.0 50 83461.5 51 82838.0 52 82959.5 53 85959.0 54 88771.5 55 84879.0 56 78017.5 57 72228.0 58 67638.5 59 60613.0 60 48889.5 61 40477.0 62 35200.5 63 26776.0 64 18952.0 65 14585.5 66 10972.0 67 8429.0 68 6615.0 69 5076.0 70 3891.5 71 2779.0 72 2027.0 73 1674.5 74 1297.0 75 944.5 76 707.0 77 481.5 78 307.0 79 193.0 80 102.0 81 64.5 82 50.0 83 40.0 84 30.5 85 20.0 86 15.0 87 13.5 88 11.0 89 9.0 90 6.5 91 5.5 92 5.0 93 3.0 94 2.5 95 3.0 96 2.5 97 1.0 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47522956282824036 2 0.04842126942602172 3 0.05648417763813756 4 0.0651167478435007 5 0.0786571752214561 6 0.1049930873708127 7 0.06818415857637086 8 0.05113811893227814 9 0.04903475157259575 10-11 0.07317965605561653 12-13 0.10668016327389129 14-15 0.054336990125128444 16-17 0.05974877906097793 18-19 0.05860945507448331 20-21 0.07427515988878446 22-23 0.06404315408699615 24-25 0.08785940742006657 26-27 0.0660588811400251 28-29 0.06651899274995562 30-31 0.07092291815929064 32-33 0.07061617708600362 34-35 0.07232516306574557 36-37 0.06811842834638078 38-39 0.040336451137242525 40-41 0.04366678279007298 42-43 0.04235217819027149 44-45 0.020946033290170484 46-47 0.027716246979148183 48-49 0.027453326059187877 50-51 0.025810070309436012 52-53 0.025744340079445937 54-55 0.024670746322941386 56-57 0.024320185096327652 58-59 0.021822436356704812 60-61 0.02874602058232602 62-63 0.019697158920359063 64-65 0.022304458043298694 66-67 0.01588480558093473 68-69 0.018645475240517868 70-71 0.04081847282383641 72-73 0.03733477063436245 74-75 0.03135331970526565 76-77 0.02322468126315974 78-79 0.015183683127707265 80-81 0.017002219490765996 82-83 0.021296594516784215 84-85 0.018031993093943835 86-87 0.00922414227527382 88-89 0.041629145660380666 90-91 0.05289092506534681 92-93 0.03409207928818543 94-95 0.05431508004846508 96-97 0.0437106029433997 98-99 0.03400443898153199 100-101 0.1669109640214631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2282055.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.05093046712427 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61894966563466 41.41325520841313 2 13.048539335833878 14.105713846773856 3 4.311116082139353 6.990595067742462 4 1.9600953492131454 4.237799097170136 5 1.0573824092576047 2.8576251539971564 6 0.6510540043696625 2.111404483231647 7 0.44988061211260527 1.7021525978664016 8 0.3137702001606261 1.3567657017230133 9 0.22458069975523723 1.0924916208055597 >10 1.2420937057933619 12.1542887201686 >50 0.07907739531616609 2.926198325971381 >100 0.03705981371112581 3.791330690592966 >500 0.0029167868518179775 1.091191865077955 >1k 0.00332189613679234 3.4559299286679765 >5k 1.6204371398987024E-4 0.7132576917976979 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8942 0.3918398110474989 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7345 0.3218590261847326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4645 0.20354461220259812 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4308 0.18877722053149465 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4221 0.1849648671920703 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3611 0.15823457366277324 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3500 0.15337053664350772 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2871 0.12580766020100304 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2767 0.12125036425502451 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2766 0.12120654410169782 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2532 0.11095262822324616 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2514 0.11016386546336525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.764030665343297E-5 2 0.0 0.0 0.0 0.0 8.764030665343297E-5 3 0.0 0.0 0.0 0.0 8.764030665343297E-5 4 0.0 0.0 0.0 0.0 8.764030665343297E-5 5 0.0 0.0 0.0 0.0 8.764030665343297E-5 6 0.0 0.0 0.0 0.0 8.764030665343297E-5 7 0.0 0.0 0.0 0.0 8.764030665343297E-5 8 0.0 0.0 0.0 0.0 8.764030665343297E-5 9 0.0 0.0 0.0 0.0 1.7528061330686595E-4 10-11 0.0 0.0 0.0 0.0 1.971906899702242E-4 12-13 0.0 0.0 0.0 0.0 3.7247130327709016E-4 14-15 0.0 0.0 0.0 0.0 4.8202168659388137E-4 16-17 0.0 0.0 0.0 0.0 5.258418399205979E-4 18-19 0.0 0.0 0.0 2.1910076663358244E-5 5.258418399205979E-4 20-21 0.0 0.0 0.0 8.764030665343297E-5 5.696619932473144E-4 22-23 0.0 0.0 0.0 1.533705366435077E-4 6.573022999007474E-4 24-25 0.0 0.0 0.0 3.067410732870154E-4 6.573022999007474E-4 26-27 0.0 0.0 0.0 0.0010078635265144793 6.792123765641056E-4 28-29 0.0 0.0 0.0 0.003417971959483886 7.011224532274638E-4 30-31 0.0 0.0 0.0 0.010801667795035615 7.011224532274638E-4 32-33 0.0 0.0 0.0 0.028132538435751985 7.887627598808968E-4 34-35 0.0 0.0 0.0 0.05470946142840554 8.764030665343298E-4 36-37 0.0 0.0 0.0 0.09936219766832964 8.764030665343298E-4 38-39 0.0 0.0 0.0 0.16557444934499826 9.640433731877627E-4 40-41 0.0 0.0 0.0 0.23621253650766522 9.640433731877627E-4 42-43 0.0 0.0 0.0 0.3101590452464993 9.859534498511211E-4 44-45 0.0 0.0 0.0 0.39931114718970406 0.0010297736031778374 46-47 0.0 0.0 0.0 0.5006671618343992 0.0010735937565045542 48-49 0.0 0.0 0.0 0.6099765343078936 0.0011174139098312705 50-51 0.0 0.0 0.0 0.7296493730431564 0.0011831441398213452 52-53 0.0 0.0 0.0 0.8381918928334331 0.0011831441398213452 54-55 0.0 0.0 0.0 0.9526063131694897 0.0012050542164847035 56-57 0.0 0.0 0.0 1.0891060907822117 0.0012707844464747782 58-59 0.0 0.0 0.0 1.2299659736509416 0.0013584247531282113 60-61 2.1910076663358244E-5 0.0 0.0 1.3844539242042808 0.0014022449064549276 62-63 4.382015332671649E-5 0.0 0.0 1.5333328951318002 0.0014022449064549276 64-65 4.382015332671649E-5 0.0 0.0 1.6811820924561416 0.0014022449064549276 66-67 4.382015332671649E-5 0.0 0.0 1.8532200144168303 0.0014022449064549276 68-69 4.382015332671649E-5 0.0 0.0 2.047496664190828 0.0014022449064549276 70-71 4.382015332671649E-5 0.0 0.0 2.248302516810506 0.0014679751364450023 72-73 4.382015332671649E-5 0.0 0.0 2.4531398235362425 0.0015775255197617937 74-75 4.382015332671649E-5 0.0 0.0 2.6554574714456924 0.0016432557497518684 76-77 4.382015332671649E-5 0.0 0.0 2.8721481296463054 0.0016651658264152265 78-79 4.382015332671649E-5 0.0 0.0 3.118592671955759 0.0016870759030785847 80-81 4.382015332671649E-5 4.382015332671649E-5 0.0 3.3736916945472393 0.0017528061330686596 82-83 4.382015332671649E-5 4.382015332671649E-5 0.0 3.632756441014787 0.0018623565163854508 84-85 6.573022999007474E-5 8.764030665343297E-5 0.0 3.895633540821759 0.0018842665930488092 86-87 8.764030665343297E-5 8.764030665343297E-5 0.0 4.164733102401126 0.0019719068997022422 88-89 8.764030665343297E-5 8.764030665343297E-5 0.0 4.454077574817434 0.0021033673596823916 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3980 0.0 53.96944 1 GTATCAA 7225 0.0 42.54676 1 ATCAACG 8900 0.0 34.38706 3 TCAACGC 8950 0.0 34.194954 4 TATCAAC 8980 0.0 34.133636 2 CAACGCA 9190 0.0 33.25023 5 AACGCAG 9470 0.0 32.267117 6 TGGTATC 1835 0.0 30.55942 2 GTGGTAT 2060 0.0 29.427608 1 ACGCAGA 10890 0.0 28.16052 7 CGCAGAG 10915 0.0 27.92178 8 GTACATG 7250 0.0 25.0845 1 TACATGG 7170 0.0 24.854858 2 GCAGAGT 12300 0.0 24.466356 9 AGGCGAC 960 0.0 23.761244 5 TAAGGTG 1360 0.0 23.411814 5 ACATGGG 7225 0.0 23.218601 3 GAGTACT 8210 0.0 21.914255 12-13 GTATAGG 590 0.0 21.843903 1 CAGAGTA 11575 0.0 21.480139 10-11 >>END_MODULE