FastQCFastQC Report
Thu 26 May 2016
SRR937929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282055
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87290.38250611838890825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76720.3361882163225689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.20661202293546826No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41500.18185363630587342No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA40130.17585027530011327No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36040.15792783258948623No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35300.1546851412433092No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29510.12931327246714036No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27750.12160092548163826No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA26310.11529082340259109No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25850.11327509634956212No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25240.11060206699663243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40400.054.2089961
GTATCAA70000.040.3804131
ATCAACG90300.030.7725053
TATCAAC90900.030.730192
TCAACGC91400.030.4008264
CAACGCA91900.030.2354225
AACGCAG94750.029.3760956
TGGTATC18450.029.3535272
GTGGTAT20950.027.2112831
ACGCAGA109350.025.5407837
CGCAGAG109700.025.32948
GTACATG73900.024.556741
TACATGG71350.024.2359052
TAAGGTG14000.024.0882915
GTAAGGT13200.023.749024
ACATGGG69650.023.5966153
TATACCG1503.0358717E-522.165755
GCAGAGT124650.021.9485539
AGGTAAG13150.021.3146442
GTGTAAG9300.020.9436661
Produced by FastQC (version 0.11.5)