FastQCFastQC Report
Thu 26 May 2016
SRR937928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195773
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83300.3793652622561622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68260.31087002162791877No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA41620.18954600498321092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41250.18786094919647886No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41130.1873144446169982No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36920.16814124228688485No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35170.16017138383612514No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28160.12824640798479625No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27240.12405653954211113No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26010.11845486760243432No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG24700.11248885927643705No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23540.1072059816747906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38800.051.5342451
GTATCAA66650.039.500571
TCAACGC86300.030.3105494
ATCAACG86750.030.2081413
TATCAAC87150.030.0149232
CAACGCA88600.029.5237055
AACGCAG91500.028.632776
TGGTATC18950.026.1015342
ACGCAGA102950.025.3010437
CGCAGAG104900.024.7842778
GTACATG68100.024.6078641
GTGGTAT21950.024.075031
GTATAGG5200.023.8039131
TACATGG66450.023.475822
ACATGGG66250.022.8288023
GCAGAGT115850.022.2360539
GTGTAAG8850.022.0556031
GTGTACG2901.03682396E-1021.3414381
GAGTACT77850.021.216112-13
AGAGTAC104400.020.41675410-11
Produced by FastQC (version 0.11.5)