##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937928_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2195773 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23665925393927 33.0 31.0 34.0 31.0 34.0 2 32.35614883687886 34.0 31.0 34.0 31.0 34.0 3 32.39213206465331 34.0 31.0 34.0 31.0 34.0 4 35.9019188231206 37.0 35.0 37.0 35.0 37.0 5 35.90354239714215 37.0 35.0 37.0 35.0 37.0 6 35.938308741386294 37.0 37.0 37.0 35.0 37.0 7 35.88137207261406 37.0 36.0 37.0 35.0 37.0 8 35.87200862748563 37.0 36.0 37.0 35.0 37.0 9 37.563860654084 39.0 38.0 39.0 35.0 39.0 10-11 37.48384646318176 39.0 38.0 39.0 35.0 39.0 12-13 37.34717614252475 39.0 37.0 39.0 35.0 39.0 14-15 38.60060534490587 40.0 38.0 41.0 34.5 41.0 16-17 38.430438620021285 40.0 38.0 41.0 34.0 41.0 18-19 38.59029166493987 40.0 38.0 41.0 34.5 41.0 20-21 38.57909196442438 40.0 38.0 41.0 34.0 41.0 22-23 38.47835181505556 40.0 38.0 41.0 34.0 41.0 24-25 38.36462489519636 40.0 38.0 41.0 34.0 41.0 26-27 38.1953696944083 40.0 38.0 41.0 34.0 41.0 28-29 38.02844647420294 40.0 38.0 41.0 33.5 41.0 30-31 37.81661879438357 40.0 37.5 41.0 33.0 41.0 32-33 37.54234363023865 40.0 37.0 41.0 32.5 41.0 34-35 37.31314325296832 40.0 37.0 41.0 32.0 41.0 36-37 37.05605383616613 39.5 36.5 41.0 31.0 41.0 38-39 36.75895960101522 39.0 36.0 41.0 30.5 41.0 40-41 36.65729517577637 39.0 36.0 41.0 30.0 41.0 42-43 36.44519424366726 39.0 35.5 41.0 30.0 41.0 44-45 36.14177535656008 39.0 35.0 41.0 29.5 41.0 46-47 35.784407131338256 39.0 35.0 40.0 28.5 41.0 48-49 35.383857529899494 39.0 35.0 40.0 27.0 41.0 50-51 34.644731490914594 37.5 34.0 39.5 25.5 40.5 52-53 34.70803197780462 38.0 34.0 40.0 26.0 40.5 54-55 34.7818028093068 38.0 34.0 40.0 26.0 41.0 56-57 34.45625230841257 38.0 34.0 40.0 24.5 41.0 58-59 34.03367583989784 37.5 33.5 40.0 23.5 41.0 60-61 33.52123238604355 37.0 33.0 40.0 21.5 41.0 62-63 32.9784613436817 36.0 32.5 39.0 19.5 41.0 64-65 32.443229787414275 35.5 32.0 39.0 17.5 40.5 66-67 31.87743815048277 35.0 31.0 38.5 12.0 40.0 68-69 31.20653774320023 35.0 30.5 38.0 7.5 40.0 70-71 30.611011930650392 35.0 30.0 37.0 4.0 39.5 72-73 29.926818938023192 34.0 29.0 36.5 2.0 39.0 74-75 29.270339648041944 34.0 28.5 36.0 2.0 39.0 76-77 28.540850078764972 34.0 27.0 35.5 2.0 38.0 78-79 27.79564508717431 33.0 26.0 35.0 2.0 37.0 80-81 27.09179660192561 33.0 25.0 35.0 2.0 36.5 82-83 26.36494164014222 32.0 23.5 35.0 2.0 36.0 84-85 25.661223405151624 32.0 21.0 35.0 2.0 35.5 86-87 24.93759168183596 31.0 18.5 34.0 2.0 35.0 88-89 24.054008542777417 31.0 11.0 34.0 2.0 35.0 90-91 23.165066243186338 30.0 4.5 34.0 2.0 35.0 92-93 22.222823124248272 29.5 2.0 34.0 2.0 35.0 94-95 21.1357676772599 29.0 2.0 34.0 2.0 35.0 96-97 19.856760694297634 28.0 2.0 34.0 2.0 35.0 98-99 18.25548656441262 24.5 2.0 33.0 2.0 35.0 100-101 14.163431283652727 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12277988273420704 1101 2 0.07915902860288782 1101 3 0.07311937662476709 1101 4 -0.06223774513441782 1101 5 0.07782849169152684 1101 6 0.07790519464609957 1101 7 0.06639262886643138 1101 8 0.08284083363346895 1101 9 -0.0558809771439428 1101 10-11 0.10765179045480266 1101 12-13 0.06300937797853123 1101 14-15 0.11973960361448377 1101 16-17 0.15453832045373872 1101 18-19 0.08475468091361194 1101 20-21 0.2032774254029306 1101 22-23 0.19034189401183 1101 24-25 0.12761327167296344 1101 26-27 0.24202758272586067 1101 28-29 0.20383389002608965 1101 30-31 0.09577742780380305 1101 32-33 0.2282681754636542 1101 34-35 0.2627118035220022 1101 36-37 0.21579411301946294 1101 38-39 0.2865668866218485 1101 40-41 0.1834247747765474 1101 42-43 0.14437750287806494 1101 44-45 0.3413611977310538 1101 46-47 0.3082407303040924 1101 48-49 0.432932752598262 1101 50-51 0.3361824182623465 1101 52-53 0.1965467542483168 1101 54-55 0.1739168367095587 1101 56-57 0.09970814262911887 1101 58-59 0.20582791960677582 1101 60-61 0.18097031945340092 1101 62-63 0.17358483290211524 1101 64-65 0.12487272273173033 1101 66-67 -0.022966596046774868 1101 68-69 -0.04423758497200936 1101 70-71 -0.07460846498065266 1101 72-73 0.008774235135000197 1101 74-75 -0.14464749911827823 1101 76-77 -0.2369820880757736 1101 78-79 -0.4931148177792082 1101 80-81 -0.6570008481902612 1101 82-83 -0.6203517522048863 1101 84-85 -0.7340351960286284 1101 86-87 -0.4364955438114144 1101 88-89 -0.6576244612820936 1101 90-91 -0.7412580154882313 1101 92-93 -0.752686289944215 1101 94-95 -0.9217475387076384 1101 96-97 -1.0844551781097742 1101 98-99 -1.3171774023284364 1101 100-101 -1.2275547762404617 1102 1 0.18108362504688102 1102 2 0.11940987662141822 1102 3 0.22219612574917136 1102 4 0.10751333729574242 1102 5 0.12809847872994595 1102 6 0.07184755696373912 1102 7 0.07561201057129097 1102 8 0.08787257572552676 1102 9 0.04297343944688947 1102 10-11 0.09502301573816396 1102 12-13 0.09864490926558744 1102 14-15 0.14102120092289283 1102 16-17 0.15694807197741056 1102 18-19 0.19129890197756794 1102 20-21 0.1488449497403721 1102 22-23 0.2239255238069333 1102 24-25 0.14501003403472623 1102 26-27 0.05307636792208115 1102 28-29 0.025887556499711195 1102 30-31 0.09661590440401113 1102 32-33 -0.040111957461526515 1102 34-35 0.07902597025761793 1102 36-37 0.17354221704306383 1102 38-39 -0.01819779168267388 1102 40-41 0.15157027545220814 1102 42-43 0.21227667830069663 1102 44-45 0.04155236730719025 1102 46-47 0.2253304961682474 1102 48-49 0.06194177055143513 1102 50-51 0.09424093843553294 1102 52-53 0.05385405410215327 1102 54-55 0.10668239750490471 1102 56-57 0.042093184409544904 1102 58-59 0.020161004739563282 1102 60-61 0.12836779883895844 1102 62-63 0.004330813432169123 1102 64-65 -0.03932694081863275 1102 66-67 0.055744195161413046 1102 68-69 -0.13024514129974207 1102 70-71 -0.3876428545451951 1102 72-73 -0.3924175687314104 1102 74-75 -0.19636300613683488 1102 76-77 -0.34149073372812566 1102 78-79 -0.43243110800838025 1102 80-81 -0.33010263799197404 1102 82-83 -0.3993713048923446 1102 84-85 -0.28601522967359116 1102 86-87 -0.0818940711548386 1102 88-89 -0.13371480628085308 1102 90-91 -0.4189001103008394 1102 92-93 -0.45034835111185956 1102 94-95 -0.6516982846881696 1102 96-97 -0.9654617692131637 1102 98-99 -1.2154283055026482 1102 100-101 -1.3413703908125925 1103 1 0.15788673907361073 1103 2 0.2071712071601084 1103 3 0.22015393395343352 1103 4 0.18046300656650516 1103 5 0.18084421191446864 1103 6 0.13600500041312102 1103 7 0.14387104097660597 1103 8 0.1407197869839294 1103 9 0.13142595326129936 1103 10-11 0.2044479551725189 1103 12-13 0.1783313289514652 1103 14-15 0.24767487433117452 1103 16-17 0.249090598585461 1103 18-19 0.06466090758499377 1103 20-21 0.2226395437464106 1103 22-23 0.2927119788794741 1103 24-25 0.19615171680686672 1103 26-27 0.21649526440246092 1103 28-29 0.2908194779957469 1103 30-31 0.21632987124942105 1103 32-33 0.17543487543174052 1103 34-35 0.02169532268160168 1103 36-37 -0.014308396779505017 1103 38-39 0.08535786065693429 1103 40-41 0.20849087321467863 1103 42-43 0.2088152089373807 1103 44-45 0.21842189938656276 1103 46-47 0.202569682819302 1103 48-49 0.24355072065623773 1103 50-51 0.32967605834189584 1103 52-53 0.42261198207667405 1103 54-55 0.26799649500878076 1103 56-57 0.3313190874500762 1103 58-59 0.40649783137211415 1103 60-61 0.42483022092024925 1103 62-63 0.33222748436337923 1103 64-65 0.19936832553408834 1103 66-67 0.20463103948729255 1103 68-69 0.22655427073716794 1103 70-71 0.12666098082513955 1103 72-73 0.07951187569791074 1103 74-75 0.12473430719849432 1103 76-77 0.033278463538930936 1103 78-79 -0.12064146813577281 1103 80-81 -0.08518476228084282 1103 82-83 -0.20655817371669016 1103 84-85 -0.1821603694509264 1103 86-87 -0.3476933470891872 1103 88-89 -0.2826027394272721 1103 90-91 -0.10094654849666185 1103 92-93 -0.15730841861873301 1103 94-95 -0.19152603327950501 1103 96-97 -0.24677053149642347 1103 98-99 -0.2711380259479732 1103 100-101 -0.9377567043384651 1104 1 0.2066533195913678 1104 2 0.19580416767117015 1104 3 0.18690947775664313 1104 4 0.13617973718903187 1104 5 0.1814958376735234 1104 6 0.23112620175498932 1104 7 0.1991270165736978 1104 8 0.2242321862363994 1104 9 0.28353366353652376 1104 10-11 0.253014745763501 1104 12-13 0.2940617426656189 1104 14-15 0.34354434351326546 1104 16-17 0.26447641664861266 1104 18-19 0.23502305830676562 1104 20-21 0.30827565535595625 1104 22-23 0.37343172628792587 1104 24-25 0.2898749133197782 1104 26-27 0.26048199684030493 1104 28-29 0.22230265587278808 1104 30-31 0.19870900831223537 1104 32-33 0.19415028076709717 1104 34-35 0.1409392689104081 1104 36-37 0.15142563707775025 1104 38-39 0.10589771318594643 1104 40-41 0.3202580172155791 1104 42-43 0.27495721718291577 1104 44-45 0.3338074026546636 1104 46-47 0.1788340309154819 1104 48-49 0.252072222732302 1104 50-51 0.14463176143139833 1104 52-53 0.16956167048203952 1104 54-55 -0.011181281299947443 1104 56-57 0.10827325643835906 1104 58-59 0.10350580932806963 1104 60-61 0.18551599144934272 1104 62-63 0.3531690153796134 1104 64-65 0.010731482550710325 1104 66-67 -0.047839104825254 1104 68-69 -0.06265277101829625 1104 70-71 -0.18944624256707243 1104 72-73 -0.182022477361528 1104 74-75 -0.35845290876455493 1104 76-77 -0.509894628619417 1104 78-79 -0.6228544773561211 1104 80-81 -0.592569224626633 1104 82-83 -0.9531186705052583 1104 84-85 -0.8961131355025884 1104 86-87 -0.8168824665129826 1104 88-89 -0.8495846208733582 1104 90-91 -1.0474235196645871 1104 92-93 -1.1321480043064724 1104 94-95 -1.1220613632529286 1104 96-97 -1.4750442688796994 1104 98-99 -1.6690828339807027 1104 100-101 -1.8936665092599707 1105 1 0.20912419299820328 1105 2 0.1687366026850441 1105 3 0.3045402344096573 1105 4 0.1565572174405574 1105 5 0.18497885462539188 1105 6 0.19203940742718828 1105 7 0.22967245168357664 1105 8 0.29326164291940415 1105 9 0.31587969666824023 1105 10-11 0.289401632833183 1105 12-13 0.35842460988438063 1105 14-15 0.3868556939518797 1105 16-17 0.3106887965518297 1105 18-19 0.33452431086657697 1105 20-21 0.3387915951816183 1105 22-23 0.3498953802785465 1105 24-25 0.2728029834274963 1105 26-27 0.34473220790975034 1105 28-29 0.2724437482474684 1105 30-31 0.07556144578074964 1105 32-33 0.08147799811913359 1105 34-35 0.06970211989686703 1105 36-37 0.24471565787291638 1105 38-39 0.07840127884907844 1105 40-41 0.21726088063361004 1105 42-43 0.2080970495744765 1105 44-45 0.16495432381217512 1105 46-47 0.23267935487366742 1105 48-49 0.07054854385874876 1105 50-51 0.09457677945024301 1105 52-53 0.2204486570240718 1105 54-55 0.24478997960365234 1105 56-57 0.11008691948541838 1105 58-59 0.1542492785475531 1105 60-61 0.12053253553241916 1105 62-63 0.05709842069331472 1105 64-65 -0.03569924163770111 1105 66-67 0.018710174569324778 1105 68-69 -0.11052757640385735 1105 70-71 -0.22474507037696867 1105 72-73 -0.29916181090667493 1105 74-75 -0.638892289521948 1105 76-77 -0.6439125890700019 1105 78-79 -0.636477735476479 1105 80-81 -0.3733951123802477 1105 82-83 -0.727574863574656 1105 84-85 -0.6133293745371233 1105 86-87 -0.6135167378557433 1105 88-89 -0.6145573872241847 1105 90-91 -0.8269556729885927 1105 92-93 -1.1452530164262598 1105 94-95 -1.3002414844211359 1105 96-97 -1.4574929911301275 1105 98-99 -1.733330788363972 1105 100-101 -1.6891112694425239 1106 1 0.1718072350388269 1106 2 0.21286998943366342 1106 3 0.27452885989657005 1106 4 0.2472621750816444 1106 5 0.18679679894317047 1106 6 0.17682294901729279 1106 7 0.2629105911204803 1106 8 0.04910309701728721 1106 9 0.23517699737163866 1106 10-11 0.252541518186554 1106 12-13 0.39269809218258445 1106 14-15 0.38034271583022417 1106 16-17 0.05692699716181693 1106 18-19 0.3833927468917224 1106 20-21 0.49962769318548794 1106 22-23 0.4087840126186819 1106 24-25 0.26384273718975493 1106 26-27 0.23106124364264957 1106 28-29 0.10412694161097136 1106 30-31 0.07386055821917381 1106 32-33 0.2319016791838564 1106 34-35 0.2896406110559795 1106 36-37 0.22688864982060863 1106 38-39 0.3229300828601893 1106 40-41 0.4210538185006172 1106 42-43 0.3211853747613631 1106 44-45 0.370995372327954 1106 46-47 0.3564297337558031 1106 48-49 0.37506778555174236 1106 50-51 0.2591588086394623 1106 52-53 0.3176171483809611 1106 54-55 0.15128129417632152 1106 56-57 0.08136845869207576 1106 58-59 0.19450576392880237 1106 60-61 0.5250243413295124 1106 62-63 0.35073011920294306 1106 64-65 -0.10831398600551978 1106 66-67 0.025815105717409637 1106 68-69 0.12027615046248386 1106 70-71 -0.03990405828907839 1106 72-73 -0.03555907991965768 1106 74-75 -0.010587390938173513 1106 76-77 -0.5654531709685671 1106 78-79 -0.2638980391433208 1106 80-81 -0.3730312935427129 1106 82-83 -0.4550717945718823 1106 84-85 -0.4123498233635452 1106 86-87 -0.5098220448733066 1106 88-89 -0.6730759914293074 1106 90-91 -0.5757562436920267 1106 92-93 -0.5092387000860157 1106 94-95 -0.6361939147631688 1106 96-97 -0.6092949062614466 1106 98-99 -0.9335199189657359 1106 100-101 -1.1348369500390216 1107 1 -0.13285737978355883 1107 2 -0.10694934168032688 1107 3 -0.1006838977900486 1107 4 -0.05659994519941591 1107 5 -0.03102468533351299 1107 6 -0.016817622155009815 1107 7 -0.01005878363217505 1107 8 0.05792460720923032 1107 9 0.06117514113631728 1107 10-11 0.021126931585449427 1107 12-13 0.006632551175208334 1107 14-15 0.07417776125439701 1107 16-17 0.06965336584985948 1107 18-19 0.07276421840843028 1107 20-21 0.05008634934314671 1107 22-23 0.14995283910987212 1107 24-25 0.12513724390158387 1107 26-27 0.005610021650348074 1107 28-29 -0.03563229245696675 1107 30-31 -0.12311319840843993 1107 32-33 0.02531226341482551 1107 34-35 0.07088277333733117 1107 36-37 0.07150921309533942 1107 38-39 -0.04022373386445821 1107 40-41 0.131984369560044 1107 42-43 -0.1070297974367449 1107 44-45 -0.17088654106149903 1107 46-47 -0.036120990970999856 1107 48-49 -0.018888183105723044 1107 50-51 -0.088503832670078 1107 52-53 -0.262808771094619 1107 54-55 -0.3234119064943073 1107 56-57 -0.3310518024722384 1107 58-59 -0.23397910265812527 1107 60-61 -0.24323899811074767 1107 62-63 -0.27301612033925693 1107 64-65 -0.22033049622162793 1107 66-67 -0.25526519600648 1107 68-69 -0.2382886888563256 1107 70-71 -0.35474140050797587 1107 72-73 -0.39839192078986585 1107 74-75 -0.6489101949164748 1107 76-77 -0.6081517444433793 1107 78-79 -0.7292792064807827 1107 80-81 -0.681449228483114 1107 82-83 -0.7669856540083977 1107 84-85 -0.8727537236539256 1107 86-87 -0.8883235147955766 1107 88-89 -0.8206055205490514 1107 90-91 -0.7659456930191304 1107 92-93 -0.7728780325967932 1107 94-95 -1.0627094412975389 1107 96-97 -1.2276411086438088 1107 98-99 -1.5337612383423078 1107 100-101 -1.6590222555601581 1108 1 -0.03636920701342916 1108 2 -0.09181480346984472 1108 3 -0.049211671096379916 1108 4 0.015843463275309944 1108 5 0.010065545489190697 1108 6 -0.003802080969002475 1108 7 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-0.06652406052648274 2316 90-91 0.11301965342054743 2316 92-93 0.13142423072422105 2316 94-95 0.17508045023676644 2316 96-97 0.2013829268242482 2316 98-99 -0.0688105353820454 2316 100-101 0.1965065474103973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5558.0 3 1435.0 4 1336.0 5 1383.0 6 1698.0 7 2159.0 8 2848.0 9 3473.0 10 4473.0 11 6026.0 12 7780.0 13 10056.0 14 12195.0 15 15175.0 16 18339.0 17 23211.0 18 23194.0 19 20703.0 20 20815.0 21 22180.0 22 24726.0 23 27886.0 24 31470.0 25 35644.0 26 40848.0 27 46988.0 28 55024.0 29 64710.0 30 76385.0 31 93008.0 32 114487.0 33 143783.0 34 187168.0 35 248376.0 36 312403.0 37 322672.0 38 156379.0 39 9779.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.770800688081344 17.595600803369628 13.564843275427723 24.0687552331213 2 16.0100169683361 18.900238577357204 39.750332167481666 25.339412286825024 3 19.74232444387481 22.460292084597715 29.086678036002695 28.71070543552478 4 13.08346097355556 15.415640490959243 35.47133555523839 36.02956298024681 5 14.07997685665775 36.712678052473144 33.96554690063101 15.24179819023809 6 34.40823023935811 34.617216845963185 16.252632866201118 14.72192004847759 7 30.02548568545637 32.09075221317656 19.356439685778422 18.527322415588646 8 27.380899755594818 34.341958531782474 18.792584629224628 19.484557083398084 9 26.50433810400142 16.341894028791806 19.470353705490435 37.68341416171634 10-11 25.829643634971163 25.64654342219381 26.3848477906827 22.138965152152334 12-13 26.561190816645343 22.92561500972658 27.040997607693697 23.47219656593438 14-15 22.93115266061924 24.54593007320847 25.17499483532084 27.347922430851447 16-17 22.836517907285835 27.72910793850796 26.22881368492361 23.205560469282595 18-19 22.978936666352773 25.958913058199773 28.493836776321984 22.568313499125466 20-21 23.07720897059266 25.2626257835679 29.055741969141586 22.604423276697855 22-23 22.781156419514364 25.277708379677954 28.924359873828546 23.01677532697913 24-25 22.72534032524722 25.498190979198824 28.971122984890023 22.805345710663925 26-27 22.16611111731893 25.93777315206393 28.710176096638907 23.18593963397823 28-29 22.397401973234523 26.455724427497973 28.49500854746064 22.65186505180687 30-31 22.47822550113884 25.811302795737845 28.47055755891303 23.23991414421028 32-33 22.070616012606973 25.887955343007434 28.551661778546254 23.489766865839336 34-35 23.00691932453681 26.04486723507584 28.347327801489985 22.600885638897367 36-37 23.176953063129073 25.502271580010095 28.287626355569788 23.033149001291044 38-39 22.50670477047951 25.89923206115079 28.325752971012975 23.26831019735673 40-41 23.027541330222554 25.7492071159069 28.445810473360915 22.777441080509632 42-43 22.67610652859733 25.77587672189835 28.23908737165936 23.308929377844958 44-45 22.667103428198363 26.117921968816503 28.526116623529628 22.688857979455506 46-47 23.24030122945451 26.36584729612277 27.65929768564558 22.73455378877714 48-49 22.86293687272923 26.492186003260777 27.530171984697994 23.114705139312004 50-51 23.031886645310028 26.75357867270321 27.559613028781342 22.65492165320542 52-53 23.418664733987466 26.2493306496303 27.58823637936057 22.743768237021662 54-55 22.715375195785274 26.61438131852485 27.776642460486972 22.893601025202905 56-57 22.85628971099235 26.792609086221255 27.42693724046071 22.924163962325693 58-59 22.814202763903985 26.260322948322205 27.475030021385756 23.450444266388054 60-61 22.416673500441917 26.54067463029276 27.93524314572342 23.1074087235419 62-63 22.607271628728004 26.543545430478545 28.022399128057053 22.8267838127364 64-65 22.311790786341728 26.623162875651307 27.81770231916696 23.24734401884 66-67 22.292712729340295 26.608964175281567 27.783006983799535 23.315316111578603 68-69 22.983656245919875 26.138431211974343 27.92818473293262 22.949727809173165 70-71 22.65938282521036 26.436307358485795 27.46219806122172 23.442111755082124 72-73 22.643175138238732 26.52527369229953 27.54363026495861 23.287920904503128 74-75 22.595815640320023 26.933853948351587 27.41532412385413 23.05500628747426 76-77 22.80145991220029 26.539040124809965 27.42792395246745 23.2315760105223 78-79 22.921308188224813 26.76828021177865 27.31799731336471 22.992414286631828 80-81 23.108442121048405 26.812136545474917 27.003412816922324 23.07600851655435 82-83 22.9116280319779 26.854363709121436 27.13477732375815 23.099230935142508 84-85 22.813494925771806 27.060500597055103 27.058587469882482 23.067417007290608 86-87 23.115788025597713 27.059805049436868 26.697139697108497 23.127267227856926 88-89 22.930989971702203 27.084265181269725 26.551326013442306 23.433418833585762 90-91 22.931678661064975 27.03285104986129 26.653825023878618 23.381645265195118 92-93 23.224238545913316 26.576001608014916 26.877276511336895 23.322483334734876 94-95 22.918184451246955 26.36502623255882 27.130502394007266 23.586286922186957 96-97 23.307783084700908 25.961586444172376 26.941236706772788 23.789393764353928 98-99 22.95813655924366 26.398409889162494 26.516542693674005 24.126910857919842 100-101 23.961172256216777 25.178067655562806 26.665219583144186 24.19554050507623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 534.0 1 393.5 2 389.5 3 551.0 4 465.5 5 415.5 6 806.5 7 1640.5 8 2621.0 9 3032.5 10 2555.0 11 2013.5 12 1892.5 13 1938.5 14 2097.5 15 2280.5 16 2435.0 17 2660.5 18 2911.0 19 3272.5 20 3708.0 21 4307.0 22 5301.0 23 6424.5 24 7905.5 25 10041.5 26 12765.0 27 15714.5 28 18313.5 29 20866.0 30 23581.5 31 27917.5 32 35139.5 33 37409.5 34 36257.0 35 38246.5 36 41298.5 37 45074.0 38 48258.5 39 50699.5 40 53910.5 41 58316.0 42 60789.5 43 62985.5 44 66247.0 45 71097.5 46 74891.5 47 77322.0 48 79606.5 49 79543.5 50 79735.0 51 79329.0 52 79413.0 53 82139.0 54 84307.5 55 81032.5 56 75101.5 57 69887.5 58 65630.0 59 58839.0 60 48094.0 61 39934.0 62 34486.5 63 26915.5 64 19650.0 65 15187.5 66 11491.5 67 8704.5 68 6680.5 69 5170.5 70 4061.5 71 3007.5 72 2184.0 73 1708.0 74 1323.5 75 985.5 76 731.0 77 505.5 78 346.5 79 213.5 80 122.0 81 85.0 82 54.5 83 37.5 84 26.5 85 15.5 86 13.0 87 9.0 88 8.0 89 7.0 90 4.5 91 4.0 92 4.5 93 2.5 94 2.5 95 3.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13703602330477696 2 0.04995962697419087 3 0.15147285261272453 4 0.12569605328055314 5 0.1919141914942938 6 0.19332599499128553 7 0.16222077600917761 8 0.06676464279322133 9 0.12064088592035697 10-11 0.09862130557211515 12-13 0.15156393670930465 14-15 0.1363528925804261 16-17 0.13373422480374791 18-19 0.13145712238924515 20-21 0.13293723895867196 22-23 0.19630899915428415 24-25 0.21270413653870415 26-27 0.14566624145574247 28-29 0.12405653954211113 30-31 0.09538782014352121 32-33 0.12300907243143987 34-35 0.09060590507306537 36-37 0.08268158867059573 38-39 0.04859336552548921 40-41 0.057064186507439524 42-43 0.060115503742873236 44-45 0.03779990008074605 46-47 0.05011902414320606 48-49 0.040873988340324796 50-51 0.051895164026518226 52-53 0.024934271438805375 54-55 0.04943589341885522 56-57 0.024456079931759796 58-59 0.04952697751543533 60-61 0.036114844294013994 62-63 0.05164468276092291 64-65 0.03955326893991319 66-67 0.038073152370486384 68-69 0.06596765694814537 70-71 0.052077332219678446 72-73 0.050528902577816566 74-75 0.04249073105462177 76-77 0.019833562030319163 78-79 0.021040426310005633 80-81 0.023636323062538798 82-83 0.020243440464929662 84-85 0.018877179016228 86-87 0.022702711072592658 88-89 0.06523898417550447 90-91 0.10875441131665249 92-93 0.08031340215951284 94-95 0.07507606660615647 96-97 0.06692403996223653 98-99 0.05506033638267708 100-101 0.13009086094054348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2195773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.9707169216719 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50612249114226 42.605671191570096 2 12.600020115686927 13.852642779735955 3 4.118829401737251 6.7924501527467305 4 1.8856528266225345 4.146227509792713 5 1.0095353484653178 2.774744093145419 6 0.6198235367555954 2.04432865082288 7 0.41486736768708765 1.5963889640416247 8 0.3009621461832521 1.323528395358271 9 0.22089967688776238 1.092871224565738 >10 1.2065970404905508 11.99936657255739 >50 0.07378942775767627 2.7896750891413213 >100 0.03659766841561596 3.7999828161118 >500 0.0029026753406406172 1.1091848499584729 >1k 0.0032344096652851557 3.381988035730268 >5k 1.6586716232231565E-4 0.6909496747213407 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8330 0.3793652622561622 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6826 0.31087002162791877 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4162 0.18954600498321092 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4125 0.18786094919647886 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4113 0.1873144446169982 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3692 0.16814124228688485 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3517 0.16017138383612514 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2816 0.12824640798479625 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2724 0.12405653954211113 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2601 0.11845486760243432 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2470 0.11248885927643705 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2354 0.1072059816747906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3662614487016644E-4 2 0.0 4.5542048290055486E-5 0.0 0.0 1.3662614487016644E-4 3 0.0 4.5542048290055486E-5 0.0 0.0 1.3662614487016644E-4 4 0.0 4.5542048290055486E-5 0.0 0.0 1.3662614487016644E-4 5 0.0 4.5542048290055486E-5 0.0 0.0 1.3662614487016644E-4 6 0.0 4.5542048290055486E-5 0.0 0.0 1.3662614487016644E-4 7 0.0 4.5542048290055486E-5 0.0 0.0 1.8216819316022194E-4 8 0.0 4.5542048290055486E-5 0.0 0.0 1.8216819316022194E-4 9 0.0 4.5542048290055486E-5 0.0 0.0 1.8216819316022194E-4 10-11 0.0 4.5542048290055486E-5 0.0 0.0 1.8216819316022194E-4 12-13 0.0 4.5542048290055486E-5 0.0 2.2771024145027743E-5 4.3264945875552707E-4 14-15 0.0 4.5542048290055486E-5 0.0 4.5542048290055486E-5 5.465045794806659E-4 16-17 0.0 4.5542048290055486E-5 0.0 9.108409658011097E-5 6.831307243508322E-4 18-19 0.0 4.5542048290055486E-5 0.0 9.108409658011097E-5 6.831307243508322E-4 20-21 0.0 4.5542048290055486E-5 0.0 1.1385512072513871E-4 8.197568692209987E-4 22-23 0.0 4.5542048290055486E-5 0.0 2.732522897403329E-4 8.425278933660265E-4 24-25 0.0 4.5542048290055486E-5 0.0 2.732522897403329E-4 8.652989175110542E-4 26-27 0.0 4.5542048290055486E-5 0.0 6.831307243508322E-4 9.336119899461374E-4 28-29 0.0 4.5542048290055486E-5 0.0 0.003165172356158856 9.563830140911652E-4 30-31 0.0 4.5542048290055486E-5 0.0 0.011840932555414426 9.79154038236193E-4 32-33 0.0 4.5542048290055486E-5 0.0 0.03017160699216176 0.001138551207251387 34-35 0.0 4.5542048290055486E-5 0.0 0.055379130720707465 0.0012524063279765259 36-37 0.0 4.5542048290055486E-5 0.0 0.1016953938316939 0.0012751773521215537 38-39 0.0 4.5542048290055486E-5 0.0 0.16923425144584617 0.001320719400411609 40-41 0.0 4.5542048290055486E-5 0.0 0.2399382814161573 0.001320719400411609 42-43 0.0 4.5542048290055486E-5 0.0 0.31248676434221573 0.0013434904245566367 44-45 0.0 4.5542048290055486E-5 0.0 0.3965118434373681 0.0013890324728466923 46-47 0.0 4.5542048290055486E-5 0.0 0.497387480399841 0.00141180349699172 48-49 0.0 4.5542048290055486E-5 0.0 0.6052538217748373 0.00141180349699172 50-51 0.0 4.5542048290055486E-5 0.0 0.7222741148561349 0.00141180349699172 52-53 2.2771024145027743E-5 4.5542048290055486E-5 0.0 0.8304592505691617 0.0014573455452817755 54-55 4.5542048290055486E-5 4.5542048290055486E-5 0.0 0.9475023146746043 0.0014573455452817755 56-57 4.5542048290055486E-5 4.5542048290055486E-5 0.0 1.0824206327338937 0.001502887593571831 58-59 4.5542048290055486E-5 4.5542048290055486E-5 0.0 1.226287963282179 0.0015484296418618864 60-61 4.5542048290055486E-5 4.5542048290055486E-5 0.0 1.3789221381263 0.001593971690151942 62-63 4.5542048290055486E-5 4.5542048290055486E-5 0.0 1.5269793371172704 0.001685055786732053 64-65 4.5542048290055486E-5 4.5542048290055486E-5 0.0 1.679340259671651 0.0017305978350221085 66-67 9.108409658011097E-5 4.5542048290055486E-5 0.0 1.8638310972946657 0.0017305978350221085 68-69 9.108409658011097E-5 4.5542048290055486E-5 0.0 2.0599123862074995 0.0017305978350221085 70-71 9.108409658011097E-5 4.5542048290055486E-5 0.0 2.2651703978507793 0.0017761398833121638 72-73 9.108409658011097E-5 4.5542048290055486E-5 0.0 2.475233095588661 0.0018216819316022193 74-75 9.108409658011097E-5 4.5542048290055486E-5 0.0 2.6760962995719506 0.0018216819316022193 76-77 9.108409658011097E-5 4.5542048290055486E-5 0.0 2.8997760697485577 0.0018672239798922748 78-79 9.108409658011097E-5 4.5542048290055486E-5 0.0 3.1492781813056268 0.0018899950040373026 80-81 9.108409658011097E-5 4.5542048290055486E-5 0.0 3.4092549639694085 0.0019355370523273581 82-83 1.8216819316022194E-4 4.5542048290055486E-5 0.0 3.670666321154327 0.002026621148907469 84-85 1.8216819316022194E-4 4.5542048290055486E-5 0.0 3.9378387474479375 0.002049392173052497 86-87 1.8216819316022194E-4 4.5542048290055486E-5 0.0 4.218013428528359 0.0021404762696326076 88-89 1.8216819316022194E-4 4.5542048290055486E-5 0.0 4.508025192039432 0.002277102414502774 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3880 0.0 51.534245 1 GTATCAA 6665 0.0 39.50057 1 TCAACGC 8630 0.0 30.310549 4 ATCAACG 8675 0.0 30.208141 3 TATCAAC 8715 0.0 30.014923 2 CAACGCA 8860 0.0 29.523705 5 AACGCAG 9150 0.0 28.63277 6 TGGTATC 1895 0.0 26.101534 2 ACGCAGA 10295 0.0 25.301043 7 CGCAGAG 10490 0.0 24.784277 8 GTACATG 6810 0.0 24.607864 1 GTGGTAT 2195 0.0 24.07503 1 GTATAGG 520 0.0 23.803913 1 TACATGG 6645 0.0 23.47582 2 ACATGGG 6625 0.0 22.828802 3 GCAGAGT 11585 0.0 22.236053 9 GTGTAAG 885 0.0 22.055603 1 GTGTACG 290 1.03682396E-10 21.341438 1 GAGTACT 7785 0.0 21.2161 12-13 AGAGTAC 10440 0.0 20.416754 10-11 >>END_MODULE