FastQCFastQC Report
Thu 26 May 2016
SRR937928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195773
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82470.3755852722480876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69740.31761024477484695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43540.19829007825490158No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39350.17920796002136832No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39060.17788724062095673No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35440.16140101913995664No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33980.15475188008960852No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28000.12751773521215537No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26820.12214377351392881No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25500.11613222313964149No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA25360.11549463446358071No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG22680.10328936552184582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.055.92061
GTATCAA69450.041.1077731
ATCAACG89050.031.7859463
TCAACGC89400.031.7131844
TATCAAC91150.031.1606752
CAACGCA91950.030.8336985
AACGCAG95550.029.7210166
TAAGGTG13700.029.1180175
TGGTATC17600.027.526432
GTAAGGT13050.026.9292494
AGGTAAG13400.026.2294582
GTGGTAT19450.026.1327951
ACGCAGA108950.025.935397
GTATACG1301.0042731E-525.5715894
CGCAGAG110150.025.5666148
GGTAAGG14400.024.735553
CCGTAAA2452.9831426E-1023.2636322
GCAGAGT123600.022.5923759
GTACATG73550.022.2176171
GAGTACT80150.022.13094712-13
Produced by FastQC (version 0.11.5)