##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937928_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2195773 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32249235235154 33.0 31.0 34.0 31.0 34.0 2 32.56423592056191 34.0 31.0 34.0 31.0 34.0 3 32.62394974343887 34.0 31.0 34.0 31.0 34.0 4 36.138566236127325 37.0 35.0 37.0 35.0 37.0 5 36.100688914564486 37.0 35.0 37.0 35.0 37.0 6 36.13136148408783 37.0 36.0 37.0 35.0 37.0 7 36.08102249185139 37.0 35.0 37.0 35.0 37.0 8 36.062431316898426 37.0 35.0 37.0 35.0 37.0 9 37.71366894483173 39.0 37.0 39.0 35.0 39.0 10-11 37.67811904964675 39.0 37.0 39.0 35.0 39.0 12-13 37.58429423260054 39.0 37.0 39.0 35.0 39.0 14-15 38.79097292844023 40.0 38.0 41.0 34.5 41.0 16-17 38.64150893557758 40.0 38.0 41.0 34.0 41.0 18-19 38.91003692093855 40.0 38.0 41.0 35.0 41.0 20-21 38.87409035451297 40.0 38.0 41.0 35.0 41.0 22-23 38.762239311622835 40.0 38.0 41.0 34.5 41.0 24-25 38.636904179074975 40.0 38.0 41.0 34.0 41.0 26-27 38.42784704976334 40.0 38.0 41.0 34.0 41.0 28-29 38.294017642078664 40.0 38.0 41.0 34.0 41.0 30-31 38.01777369518616 40.0 37.5 41.0 33.0 41.0 32-33 37.7772228732205 40.0 37.0 41.0 33.0 41.0 34-35 37.59757930350724 40.0 37.0 41.0 33.0 41.0 36-37 37.57815744159346 40.0 37.0 41.0 33.0 41.0 38-39 37.391982459024675 40.0 37.0 41.0 32.0 41.0 40-41 37.17940902816457 40.0 36.0 41.0 31.5 41.0 42-43 36.90106468200493 39.0 36.0 41.0 31.0 41.0 44-45 36.596861560826184 39.0 35.0 41.0 30.5 41.0 46-47 36.16298793181262 39.0 35.0 40.0 30.0 41.0 48-49 35.695757712659734 38.0 35.0 40.0 28.5 41.0 50-51 35.23732439555455 38.0 34.5 40.0 27.0 41.0 52-53 34.6157617385768 38.0 34.0 40.0 25.5 41.0 54-55 34.2145460846818 37.5 33.0 40.0 24.0 41.0 56-57 33.794139694768084 37.0 33.0 40.0 23.0 41.0 58-59 33.2627011079925 36.5 32.5 39.0 22.5 40.5 60-61 32.68081923768987 36.0 31.0 39.0 20.0 40.0 62-63 32.12085652751901 35.0 31.0 39.0 18.0 40.0 64-65 31.549234142144932 35.0 30.5 38.0 15.0 40.0 66-67 30.904532025851488 34.5 29.5 38.0 9.5 40.0 68-69 30.22074913936914 34.0 29.0 37.0 7.5 39.0 70-71 29.48466348752808 34.0 28.0 36.0 4.0 39.0 72-73 28.814775479979033 33.5 26.5 36.0 2.0 38.5 74-75 28.13737075735971 33.0 26.0 35.0 2.0 38.0 76-77 26.90790805789123 31.5 25.5 34.0 2.0 36.0 78-79 27.56812566690637 32.5 26.0 35.0 2.0 36.0 80-81 27.17948804361835 32.5 25.5 35.0 2.0 36.0 82-83 26.506826297618197 32.0 24.5 35.0 2.0 36.0 84-85 25.802673591486915 31.5 22.0 34.0 2.0 35.0 86-87 25.009927255686264 31.0 19.0 34.0 2.0 35.0 88-89 24.0811780179463 30.5 14.5 34.0 2.0 35.0 90-91 23.382404055428317 30.0 7.0 34.0 2.0 35.0 92-93 22.37861267990817 29.5 2.0 34.0 2.0 35.0 94-95 21.343253378195286 29.0 2.0 34.0 2.0 35.0 96-97 20.070732266040253 28.0 2.0 33.5 2.0 35.0 98-99 18.421036919572288 25.5 2.0 33.0 2.0 34.0 100-101 14.484512971058482 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13398607502522708 1101 2 0.19027371445865526 1101 3 0.12463604347477997 1101 4 0.05127031594390985 1101 5 0.09321138376252236 1101 6 -0.014382919363171709 1101 7 0.11126864361613542 1101 8 0.04942604286608088 1101 9 0.03562921763997906 1101 10-11 0.09705957745006089 1101 12-13 0.2023279711711865 1101 14-15 0.2819314570480955 1101 16-17 0.24607135478468223 1101 18-19 0.28275524400056185 1101 20-21 0.218456653801276 1101 22-23 0.1919554823811609 1101 24-25 0.34760035990505855 1101 26-27 0.3368247498068868 1101 28-29 0.32131789280173706 1101 30-31 0.31454719957559263 1101 32-33 0.36130554393128733 1101 34-35 0.21643087539941064 1101 36-37 0.3806418184358833 1101 38-39 0.32684130363800534 1101 40-41 0.2473024322627566 1101 42-43 0.22341213906593538 1101 44-45 0.30652304999146196 1101 46-47 0.1840533222536891 1101 48-49 0.17997666059077488 1101 50-51 0.21945034603106706 1101 52-53 -0.11280091358075595 1101 54-55 -0.18561633135491462 1101 56-57 -0.050035810126161095 1101 58-59 -0.019877099380160246 1101 60-61 0.05034400206873357 1101 62-63 0.1358601617617481 1101 64-65 -0.0656084693836192 1101 66-67 -0.33549381422688285 1101 68-69 -0.06844297326539461 1101 70-71 -0.1307382293472621 1101 72-73 -0.2452593274257815 1101 74-75 -0.13159724273036133 1101 76-77 -0.40243803882758655 1101 78-79 -0.577244563401667 1101 80-81 -0.5028340988159137 1101 82-83 -0.3972180721134677 1101 84-85 -0.26248670199199253 1101 86-87 -0.26008780855658387 1101 88-89 -0.48564389098227423 1101 90-91 -0.5538214078558639 1101 92-93 -0.5101657200728518 1101 94-95 -0.6070594705832484 1101 96-97 -0.3568546532321797 1101 98-99 -0.24729295625440884 1101 100-101 -0.9578546720839523 1102 1 0.14136634583960017 1102 2 0.10654651443659446 1102 3 0.09452589758802077 1102 4 0.13810101559366927 1102 5 0.11889914747560226 1102 6 0.08722244176862404 1102 7 0.15433993232853283 1102 8 0.10019728469614364 1102 9 0.138662007689895 1102 10-11 0.19315885766847174 1102 12-13 0.1735275519880446 1102 14-15 0.2062359163845784 1102 16-17 0.19367952885600204 1102 18-19 0.23947535651814178 1102 20-21 0.0900914129088548 1102 22-23 0.2484901615130113 1102 24-25 0.2840029963464161 1102 26-27 0.11099755254261368 1102 28-29 0.04055795788544003 1102 30-31 0.2546538706547139 1102 32-33 0.10984121586531614 1102 34-35 0.12023518305777259 1102 36-37 0.10685620855198152 1102 38-39 -0.05231503512816715 1102 40-41 0.043769159600671514 1102 42-43 -0.12291807797165433 1102 44-45 -0.37852170884033143 1102 46-47 -0.3365088244052501 1102 48-49 -0.21787987949360854 1102 50-51 -0.2368449574630418 1102 52-53 -0.06258707502142613 1102 54-55 -0.2566801188952468 1102 56-57 -0.25605241198490347 1102 58-59 -0.3364854664963417 1102 60-61 -0.4357529064555834 1102 62-63 -0.1281708357286604 1102 64-65 -0.149957273884322 1102 66-67 -0.2901113923411138 1102 68-69 -0.14417932912982323 1102 70-71 -0.23910622802352677 1102 72-73 -0.38770515076214807 1102 74-75 -0.5151766255383343 1102 76-77 -0.43287272071721716 1102 78-79 -0.33457513895115554 1102 80-81 -0.4134854322043999 1102 82-83 -0.4494876730621442 1102 84-85 -0.618312520364352 1102 86-87 -0.44640026546621314 1102 88-89 -0.5779955635781739 1102 90-91 -0.7158620575897352 1102 92-93 -0.9592728048195482 1102 94-95 -0.9319981098926995 1102 96-97 -1.0360265458992295 1102 98-99 -0.9791057578870159 1102 100-101 -1.154068455487609 1103 1 0.2404688310724694 1103 2 0.2322547649742006 1103 3 0.20327751040343855 1103 4 0.15339741976384857 1103 5 0.1892073381280781 1103 6 0.1391617944648189 1103 7 0.12129052709674681 1103 8 0.1789442700242887 1103 9 0.23423802097350688 1103 10-11 0.25567207322772134 1103 12-13 0.28513829864672147 1103 14-15 0.26329914963715595 1103 16-17 0.2849025465221118 1103 18-19 0.30919863277913606 1103 20-21 0.3609677501165791 1103 22-23 0.3589028789368669 1103 24-25 0.38531687608618626 1103 26-27 0.4766164109142821 1103 28-29 0.41706153738294915 1103 30-31 0.42743782144002296 1103 32-33 0.3978700696095032 1103 34-35 0.3388073038079753 1103 36-37 0.32628294454022466 1103 38-39 0.3926558661430377 1103 40-41 0.35409498914581405 1103 42-43 0.23536539645467514 1103 44-45 0.25007714245399626 1103 46-47 0.19894502321431418 1103 48-49 0.20186838519865802 1103 50-51 0.1289581293431752 1103 52-53 0.11106978318692029 1103 54-55 0.28420639878447673 1103 56-57 0.3327723695704705 1103 58-59 0.44785762085427905 1103 60-61 -0.012429826182284387 1103 62-63 0.15852584609076104 1103 64-65 0.1426720643849997 1103 66-67 0.09450074256374918 1103 68-69 0.21362049121478677 1103 70-71 0.2256879576748183 1103 72-73 -0.04688320014016156 1103 74-75 -0.17480795090513013 1103 76-77 0.06140182538072381 1103 78-79 -0.08862996106509868 1103 80-81 -0.27748967860448914 1103 82-83 -0.048765893929392234 1103 84-85 -0.3166493469969005 1103 86-87 -0.32674727257076697 1103 88-89 -0.2810554759281203 1103 90-91 -0.5240077417773428 1103 92-93 -0.5989052190068804 1103 94-95 -0.5919131818909591 1103 96-97 -0.668011084647631 1103 98-99 -0.5638789293490305 1103 100-101 -0.8856242167092656 1104 1 0.08539302317720399 1104 2 0.09697788698561283 1104 3 0.10370930428403113 1104 4 0.02195642442386969 1104 5 0.09127102956177424 1104 6 0.09799688487920122 1104 7 0.012521071450848353 1104 8 0.1287911222311564 1104 9 0.17100241051769416 1104 10-11 0.17805788394566235 1104 12-13 0.1530345547891656 1104 14-15 0.21421137323397943 1104 16-17 0.16307528715932307 1104 18-19 0.19238238599660207 1104 20-21 0.15631843705185133 1104 22-23 0.21498235839175805 1104 24-25 0.17585413680259165 1104 26-27 0.16559805941202654 1104 28-29 0.1558003214975514 1104 30-31 0.07189863522720685 1104 32-33 0.06346213089800301 1104 34-35 0.12946607541471877 1104 36-37 0.05984807196401931 1104 38-39 0.2864172577890045 1104 40-41 0.11853773369697507 1104 42-43 0.1073420260699649 1104 44-45 0.04302237546004761 1104 46-47 0.0229426895336573 1104 48-49 0.027145063817052062 1104 50-51 0.08187559742557937 1104 52-53 0.04486817374474583 1104 54-55 0.05025438824094408 1104 56-57 0.24387235792305972 1104 58-59 0.18494603943936028 1104 60-61 0.039960045391829624 1104 62-63 0.09531269101536566 1104 64-65 -0.03174412195688703 1104 66-67 -0.08807377729509369 1104 68-69 0.1569347164884256 1104 70-71 0.02800915146160321 1104 72-73 -0.3523902247917583 1104 74-75 -0.31574431928250135 1104 76-77 -0.2645408546374526 1104 78-79 -0.08543174826154498 1104 80-81 -0.11838659266858897 1104 82-83 -0.32458491195770733 1104 84-85 -0.2669453068122607 1104 86-87 -0.5023890609844166 1104 88-89 -0.7349746218681545 1104 90-91 -1.0327205343137607 1104 92-93 -0.98242286484356 1104 94-95 -0.9637708189763359 1104 96-97 -1.4084816612787172 1104 98-99 -1.5588939398264579 1104 100-101 -1.8643904509524614 1105 1 0.16334411049412978 1105 2 0.18282104135779775 1105 3 0.1194789876848148 1105 4 0.0849124259718721 1105 5 0.12143777689507118 1105 6 0.11481830281055494 1105 7 0.11425941264793948 1105 8 0.12120449962925761 1105 9 0.13933615460749849 1105 10-11 0.1825389896427012 1105 12-13 0.19189261219806752 1105 14-15 0.20149593641179564 1105 16-17 0.21352855445488927 1105 18-19 0.2206765786918652 1105 20-21 0.10985940530878935 1105 22-23 0.048867392108441265 1105 24-25 0.11990583867059712 1105 26-27 0.13197323744774536 1105 28-29 0.08496630769415248 1105 30-31 0.1758078491382875 1105 32-33 0.23451310330209196 1105 34-35 0.26617463924182516 1105 36-37 0.243502933588573 1105 38-39 0.2096839891869351 1105 40-41 0.17813483603273994 1105 42-43 0.08517394452045579 1105 44-45 0.02028220416965354 1105 46-47 0.1692530571260562 1105 48-49 0.14229297079010905 1105 50-51 0.2312436758078107 1105 52-53 0.13205415536859277 1105 54-55 0.22832226896517938 1105 56-57 0.11332484428643852 1105 58-59 0.0741462124519714 1105 60-61 -0.025985273518507768 1105 62-63 0.05878723549633236 1105 64-65 0.07860318006591527 1105 66-67 -0.14170006412584613 1105 68-69 -0.11593961875418657 1105 70-71 -0.5670348489699393 1105 72-73 -0.5695071894300803 1105 74-75 -0.3119891143051241 1105 76-77 -0.31075076010407443 1105 78-79 -0.5191508957488544 1105 80-81 -0.6967895121078591 1105 82-83 -0.7462731507626117 1105 84-85 -0.8324060320597333 1105 86-87 -0.8965234585234434 1105 88-89 -1.1437585748499046 1105 90-91 -1.115576746782743 1105 92-93 -0.9523468798445798 1105 94-95 -0.8220230155336985 1105 96-97 -1.121424548312767 1105 98-99 -1.5294760559844285 1105 100-101 -1.8962124973907084 1106 1 0.11037719317116057 1106 2 0.1626610622578326 1106 3 0.21458242768161284 1106 4 0.1268815275877202 1106 5 0.07298133925642958 1106 6 0.1029891311630351 1106 7 0.13700130962971002 1106 8 0.16750355135454242 1106 9 0.12658018781758074 1106 10-11 0.18264822728355767 1106 12-13 0.1551922144810689 1106 14-15 0.21027967761930455 1106 16-17 0.31669384733366 1106 18-19 0.22371982437905302 1106 20-21 0.30790000399544937 1106 22-23 0.36783175947991253 1106 24-25 0.44283656728796217 1106 26-27 0.3287599030044035 1106 28-29 0.3329095353973699 1106 30-31 0.2576958343316491 1106 32-33 0.34414940405102357 1106 34-35 0.27170459358278265 1106 36-37 0.2533243173972082 1106 38-39 0.2544824606878606 1106 40-41 0.3379618082091156 1106 42-43 0.19801355284716493 1106 44-45 -0.002303770453174536 1106 46-47 -0.030430475679040114 1106 48-49 -0.030647002781286403 1106 50-51 -0.16213337251294035 1106 52-53 -0.18339919923886328 1106 54-55 -0.2415290620552284 1106 56-57 -0.19410460314588107 1106 58-59 -0.26364049036047277 1106 60-61 -0.31211936030793197 1106 62-63 -0.09780549760183987 1106 64-65 -0.45017012952183677 1106 66-67 -0.49063429570688655 1106 68-69 -0.48475901052819736 1106 70-71 -0.872819982439939 1106 72-73 -0.9172418767275623 1106 74-75 -0.6241851174493256 1106 76-77 -0.7409074321782008 1106 78-79 -0.5571876722679612 1106 80-81 -0.5034603903646975 1106 82-83 -0.5450468375076944 1106 84-85 -0.4487036441479013 1106 86-87 -0.7785469213888554 1106 88-89 -0.8972361263997186 1106 90-91 -1.0319924735074224 1106 92-93 -1.2158328990363714 1106 94-95 -1.3214452059070823 1106 96-97 -1.5547579659698947 1106 98-99 -1.1578578808647642 1106 100-101 -1.4578387399976194 1107 1 -0.08963304115988535 1107 2 -2.8486138175765063E-4 1107 3 -0.012933779238110787 1107 4 -0.03892627518925451 1107 5 -0.027151707047110563 1107 6 -0.06786511922492622 1107 7 -0.07870873991235783 1107 8 0.015430396531954216 1107 9 -0.08136143344979985 1107 10-11 -0.007365086131635223 1107 12-13 -0.05879974124687948 1107 14-15 -0.0932298414066608 1107 16-17 -0.12233841979907112 1107 18-19 -0.050041942356386926 1107 20-21 -0.126924108264177 1107 22-23 -0.019967578231486982 1107 24-25 -0.10933952382130485 1107 26-27 -0.11540413443480446 1107 28-29 -0.005247696431304405 1107 30-31 -0.07943445045948039 1107 32-33 -0.14509235939888754 1107 34-35 -0.0797021437695804 1107 36-37 -0.22428127520350216 1107 38-39 -0.20384228913105318 1107 40-41 -0.5003433382538276 1107 42-43 -0.34697368937836615 1107 44-45 -0.46382136801205576 1107 46-47 -0.5522037953490297 1107 48-49 -0.5182617052543392 1107 50-51 -0.5507705637205547 1107 52-53 -0.4987102636048988 1107 54-55 -0.4422958901724954 1107 56-57 -0.4535954078410285 1107 58-59 -0.572769996815353 1107 60-61 -0.7110004360635038 1107 62-63 -0.6242210527308458 1107 64-65 -0.9391411004787358 1107 66-67 -1.0160593608574082 1107 68-69 -0.9441737385651265 1107 70-71 -1.1826342666670762 1107 72-73 -1.3005687458875705 1107 74-75 -1.49366236714636 1107 76-77 -1.3756975251700503 1107 78-79 -1.273674959955006 1107 80-81 -1.418232900512276 1107 82-83 -1.4655594676824535 1107 84-85 -1.562374997376832 1107 86-87 -1.6952630759509724 1107 88-89 -1.8735772520267915 1107 90-91 -2.0249303458470997 1107 92-93 -2.231206796924102 1107 94-95 -2.432738614514726 1107 96-97 -2.6393206635462896 1107 98-99 -2.8131961386829367 1107 100-101 -2.5654598685763954 1108 1 -0.02412180017766019 1108 2 -0.018676975296564535 1108 3 -0.1497004688438821 1108 4 0.030078200396765453 1108 5 -5.477327942102761E-4 1108 6 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1.0266348499976417 2316 72-73 1.338094504664877 2316 74-75 1.523151211035625 2316 76-77 1.3898287314117503 2316 78-79 1.5447042112826601 2316 80-81 1.609742232083068 2316 82-83 1.7436415418037186 2316 84-85 1.8534324956336796 2316 86-87 1.9710518635025345 2316 88-89 1.904631066990369 2316 90-91 2.0157889392106547 2316 92-93 2.435368100915131 2316 94-95 2.9002686880398656 2316 96-97 3.355240005909778 2316 98-99 3.691214694980321 2316 100-101 3.9429692014161564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 22.0 5 44.0 6 116.0 7 268.0 8 668.0 9 1321.0 10 2135.0 11 3585.0 12 5585.0 13 8078.0 14 10909.0 15 15007.0 16 19236.0 17 25649.0 18 26613.0 19 23252.0 20 23179.0 21 24609.0 22 27013.0 23 29549.0 24 33327.0 25 37676.0 26 43131.0 27 50548.0 28 60172.0 29 72502.0 30 85602.0 31 103349.0 32 126015.0 33 157259.0 34 201890.0 35 262134.0 36 317904.0 37 292912.0 38 101851.0 39 2658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77912381358391 18.336496502295745 14.148497232853416 23.735882451266928 2 16.38208638159447 19.450980499327265 39.13716787959448 25.02976523948378 3 20.013882366980006 22.665286931855704 29.425425773743836 27.895404927420454 4 13.505631046560824 15.927329464384524 35.21853124161742 35.34850824743723 5 13.990467927742065 36.84066228091149 34.06625086246354 15.102618928882908 6 34.32502408066511 35.02000459980553 15.967837795372414 14.687133524156948 7 29.94348687227687 32.19690742166881 19.348311505788622 18.511294200265695 8 27.387075075611182 34.71000873041066 18.56425960242703 19.338656591551132 9 26.367309538513105 16.302876619248263 19.47870726104532 37.851106581193314 10-11 25.780101130672435 25.897895638574663 26.269291042380065 22.05271218837284 12-13 26.457361525578303 23.009690277728755 27.10743331173866 23.425514884954282 14-15 22.83079808340844 24.627454659475273 25.194771955024496 27.346975302091792 16-17 22.70140324917664 27.923845671965825 26.291150692561526 23.08360038629601 18-19 22.855777896895535 26.075987818412923 28.60623570833597 22.461998576355573 20-21 22.95692678614775 25.249149160682823 29.224833350259793 22.569090702909637 22-23 22.749027335703644 25.317143438779876 29.073884231202406 22.859944994314073 24-25 22.777433797293302 25.524667651470523 29.04466207693721 22.653236474298964 26-27 22.18960819668808 25.977549616249984 28.820344774889207 23.01249741217273 28-29 22.388488979270235 26.434380031289358 28.655218194280923 22.521912795159487 30-31 22.496194270398654 25.86948823895769 28.59340006216689 23.040917428476764 32-33 22.06065927579946 25.887102173129918 28.740835232057233 23.311403319013397 34-35 22.976772975269963 26.050344577241002 28.498184314095216 22.47469813339382 36-37 23.132728257270728 25.55121081248972 28.39717691893957 22.918884011299976 38-39 22.51630444683293 25.908153134279416 28.447707357950925 23.127835060936732 40-41 23.0447855406696 25.738148252222153 28.562697343162707 22.654368863945535 42-43 22.659289845887873 25.790081688968904 28.342699718746562 23.20792874639666 44-45 22.645037224940197 26.095850093759715 28.618948456636563 22.640164224663533 46-47 23.245069727216546 26.334189676428004 27.77224947410272 22.648491122252732 48-49 22.84564862408656 26.492268392781632 27.61477292936681 23.047310053764996 50-51 23.081459984596936 26.69125145115357 27.59872057101555 22.628567993233947 52-53 23.463891982246025 26.28136721071797 27.597566002372965 22.657174804663036 54-55 22.81647629271572 26.610079017449063 27.725951691366923 22.847492998468294 56-57 22.88641085335229 26.82186248648865 27.452642530189575 22.83908412996948 58-59 22.821390200398458 26.292757179693982 27.496473558349884 23.389379061557676 60-61 22.474835170165328 26.63572247877474 27.845556272369276 23.04388607869066 62-63 22.705218173813403 26.622418694293827 27.89524067436516 22.777122457527618 64-65 22.338521037419344 26.683642674768993 27.76677424718313 23.211062040628537 66-67 22.288920651491672 26.762232321879665 27.62271493732048 23.326132089308178 68-69 22.975890800755433 26.231239751010634 27.806227103139886 22.98664234509405 70-71 22.663672195097064 26.499251382107598 27.349719570060564 23.487356852734766 72-73 22.67317711683946 26.62893143919373 27.35744024717971 23.3404511967871 74-75 22.616170636479644 27.014542581570712 27.207402088157384 23.161884693792263 76-77 22.71868307418435 26.563360861150976 27.5439810011823 23.17397506348237 78-79 22.806112036385766 26.757789010652605 27.513525308590236 22.922573644371393 80-81 22.981370420321323 26.786198233011625 27.117445924298345 23.114985422368704 82-83 22.747990781547877 26.904426556376915 27.292955565411532 23.054627096663676 84-85 22.668192688759824 27.100649128800818 27.193804805830773 23.03735337660859 86-87 22.9724512177149 27.035737723365045 26.892163604792362 23.09964745412769 88-89 22.714925736880108 27.300607907581647 26.557042867847862 23.427423487690387 90-91 22.694097688220978 27.145750592454306 26.784241854379697 23.37590986494502 92-93 22.871701333804666 26.732330032095224 27.005951430550436 23.390017203549675 94-95 22.570986413653078 26.53069311891555 27.214212428017188 23.684108039414184 96-97 22.916364898510245 26.145625796554057 27.106888330750657 23.831120974185044 98-99 22.551581902710513 26.566587215877647 26.736503803936163 24.145327077475677 100-101 23.201129396160663 25.801869679908013 26.795716438485005 24.201284485446315 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 557.0 1 396.0 2 380.5 3 557.5 4 464.5 5 409.5 6 813.0 7 1650.5 8 2583.5 9 2991.0 10 2508.5 11 1908.5 12 1809.5 13 1937.0 14 2133.5 15 2338.0 16 2519.0 17 2689.5 18 2860.5 19 3227.0 20 3732.0 21 4321.0 22 5272.5 23 6579.5 24 8105.0 25 10028.0 26 12736.5 27 15897.5 28 18568.0 29 21179.0 30 24104.0 31 28461.5 32 35659.0 33 37900.5 34 36575.5 35 38484.5 36 41687.0 37 45317.0 38 48562.0 39 51394.5 40 54425.0 41 58451.5 42 61100.5 43 63305.5 44 66486.0 45 71670.5 46 75263.5 47 77825.5 48 80072.0 49 79918.0 50 80051.5 51 79553.5 52 79816.0 53 82242.5 54 84148.0 55 80674.5 56 74954.5 57 69545.5 58 64436.0 59 57723.0 60 47225.5 61 39273.0 62 34046.5 63 26188.0 64 18954.5 65 14463.5 66 10787.0 67 8276.0 68 6491.5 69 5042.5 70 3841.0 71 2735.0 72 1973.5 73 1612.0 74 1265.0 75 938.5 76 710.0 77 479.0 78 302.0 79 185.5 80 122.0 81 77.5 82 36.5 83 19.5 84 14.0 85 13.0 86 10.5 87 5.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008652989175110542 2 0.01179539050712437 3 0.008288652788790098 4 0.0 5 0.0012751773521215537 6 0.0012751773521215537 7 0.0 8 0.0 9 4.5542048290055486E-5 10-11 0.0 12-13 7.0590174849586E-4 14-15 0.0 16-17 0.0021632472937776356 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003347340549319078 26-27 0.01814850624358711 28-29 0.006284802664027657 30-31 0.0032107144044489114 32-33 0.0 34-35 0.00837973688537021 36-37 7.286727726408878E-4 38-39 0.0024137285593729407 40-41 0.0019127660281823302 42-43 0.0030285462112886897 44-45 2.2771024145027743E-5 46-47 9.108409658011097E-5 48-49 6.148176519157491E-4 50-51 0.0048274571187458815 52-53 0.00947274604433154 54-55 0.008288652788790098 56-57 0.018034651122861973 58-59 0.026027280597766708 60-61 0.004531433804860521 62-63 0.02247500083114238 64-65 0.02359078101424874 66-67 0.0145279134045277 68-69 0.03367834471049603 70-71 0.017943567026281863 72-73 0.0056244429638218525 74-75 0.017875253953846777 76-77 0.02179187010679155 78-79 0.0286459483744449 80-81 0.013025025810955868 82-83 0.03768604496002091 84-85 0.023818491255699018 86-87 0.03367834471049603 88-89 0.02450162198004985 90-91 0.03037654620946701 92-93 0.026710411322117544 94-95 0.016873328891465554 96-97 0.01726043630193103 98-99 0.012933941714375757 100-101 0.012000329724429621 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2195773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.941813602897206 #Duplication Level Percentage of deduplicated Percentage of total 1 76.68336202084103 41.364396205716936 2 12.997778211925745 14.02247459118994 3 4.279437026630627 6.925217832475379 4 1.9474727563092418 4.202008496702143 5 1.0643562828765065 2.870665410899852 6 0.6430116960319704 2.0811130231103605 7 0.44265142010057174 1.6714194275885952 8 0.3072251370744339 1.3257824862554926 9 0.23927284121685444 1.1616129901039665 >10 1.2712792211485204 12.309319404511765 >50 0.08019893581848851 2.956721137551558 >100 0.037195689571884495 3.825824154082473 >500 0.0032948957213329295 1.2188358324989272 >1k 0.003294895721332775 3.3709503718119267 >5k 1.6896901135039872E-4 0.6936586355006739 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8247 0.3755852722480876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6974 0.31761024477484695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4354 0.19829007825490158 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3935 0.17920796002136832 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3906 0.17788724062095673 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3544 0.16140101913995664 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3398 0.15475188008960852 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2800 0.12751773521215537 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2682 0.12214377351392881 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2550 0.11613222313964149 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2536 0.11549463446358071 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2268 0.10328936552184582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.108409658011097E-5 2 0.0 0.0 0.0 0.0 9.108409658011097E-5 3 0.0 0.0 0.0 4.5542048290055486E-5 9.108409658011097E-5 4 0.0 0.0 0.0 4.5542048290055486E-5 9.108409658011097E-5 5 0.0 0.0 0.0 4.5542048290055486E-5 9.108409658011097E-5 6 0.0 0.0 0.0 4.5542048290055486E-5 9.108409658011097E-5 7 0.0 0.0 0.0 4.5542048290055486E-5 9.108409658011097E-5 8 0.0 0.0 0.0 4.5542048290055486E-5 9.108409658011097E-5 9 0.0 0.0 0.0 4.5542048290055486E-5 2.2771024145027742E-4 10-11 0.0 0.0 0.0 4.5542048290055486E-5 2.2771024145027742E-4 12-13 0.0 0.0 0.0 4.5542048290055486E-5 3.643363863204439E-4 14-15 0.0 0.0 0.0 4.5542048290055486E-5 4.781915070455826E-4 16-17 0.0 0.0 0.0 9.108409658011097E-5 6.375886760607768E-4 18-19 0.0 0.0 0.0 9.108409658011097E-5 6.603597002058045E-4 20-21 0.0 0.0 0.0 1.1385512072513871E-4 7.286727726408878E-4 22-23 0.0 0.0 0.0 2.732522897403329E-4 7.742148209309432E-4 24-25 4.5542048290055486E-5 0.0 0.0 2.732522897403329E-4 7.742148209309432E-4 26-27 4.5542048290055486E-5 0.0 0.0 6.375886760607768E-4 8.880699416560819E-4 28-29 4.5542048290055486E-5 0.0 0.0 0.0030968592837237728 9.563830140911652E-4 30-31 4.5542048290055486E-5 0.0 0.0 0.011658764362254204 9.563830140911652E-4 32-33 4.5542048290055486E-5 0.0 0.0 0.029830041629986342 0.001138551207251387 34-35 6.831307243508324E-5 0.0 0.0 0.055014794334387024 0.001320719400411609 36-37 9.108409658011097E-5 0.0 0.0 0.10144491256609858 0.0013662614487016645 38-39 9.108409658011097E-5 0.0 0.0 0.16921148042170114 0.00141180349699172 40-41 9.108409658011097E-5 0.0 0.0 0.23973334219885206 0.00141180349699172 42-43 9.108409658011097E-5 0.0 0.0 0.3123729092214905 0.0014573455452817755 44-45 9.108409658011097E-5 0.0 0.0 0.3961475070510476 0.0014573455452817755 46-47 9.108409658011097E-5 0.0 0.0 0.49679543377207025 0.0014573455452817755 48-49 9.108409658011097E-5 0.0 0.0 0.6040924995434409 0.0014573455452817755 50-51 9.108409658011097E-5 0.0 0.0 0.7202019516589374 0.0015484296418618864 52-53 9.108409658011097E-5 0.0 0.0 0.8278178117683386 0.001593971690151942 54-55 1.1385512072513871E-4 0.0 0.0 0.9445648525598958 0.001593971690151942 56-57 1.8216819316022194E-4 0.0 0.0 1.0782990773636438 0.0016395137384419974 58-59 1.8216819316022194E-4 0.0 0.0 1.2211189408012575 0.0017305978350221085 60-61 1.8216819316022194E-4 0.0 0.0 1.3725234803415471 0.0017305978350221085 62-63 1.8216819316022194E-4 0.0 0.0 1.5196926093908614 0.0017305978350221085 64-65 1.8216819316022194E-4 0.0 0.0 1.67027739206193 0.0017989109074571916 66-67 1.8216819316022194E-4 0.0 0.0 1.853561365405258 0.0018672239798922748 68-69 1.8216819316022194E-4 0.0 0.0 2.0474110939518795 0.0019127660281823304 70-71 1.8216819316022194E-4 0.0 0.0 2.2504375452289467 0.001981079100617414 72-73 1.8216819316022194E-4 0.0 0.0 2.4582231405523247 0.0020721631971975245 74-75 1.8216819316022194E-4 0.0 0.0 2.6581527325456684 0.0021404762696326076 76-77 1.8216819316022194E-4 0.0 0.0 2.881058287901345 0.0021404762696326076 78-79 1.8216819316022194E-4 0.0 0.0 3.128078357826606 0.002208789342067691 80-81 1.8216819316022194E-4 0.0 0.0 3.3856641829551597 0.0022315603662127186 82-83 1.8216819316022194E-4 0.0 0.0 3.6439331388080642 0.0023226444627928297 84-85 1.8216819316022194E-4 0.0 0.0 3.9075760563591957 0.0024592706076629963 86-87 1.8216819316022194E-4 0.0 0.0 4.18210352345165 0.0024820416318080242 88-89 1.8216819316022194E-4 0.0 0.0 4.466604699119626 0.0025048126559530518 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4035 0.0 55.9206 1 GTATCAA 6945 0.0 41.107773 1 ATCAACG 8905 0.0 31.785946 3 TCAACGC 8940 0.0 31.713184 4 TATCAAC 9115 0.0 31.160675 2 CAACGCA 9195 0.0 30.833698 5 AACGCAG 9555 0.0 29.721016 6 TAAGGTG 1370 0.0 29.118017 5 TGGTATC 1760 0.0 27.52643 2 GTAAGGT 1305 0.0 26.929249 4 AGGTAAG 1340 0.0 26.229458 2 GTGGTAT 1945 0.0 26.132795 1 ACGCAGA 10895 0.0 25.93539 7 GTATACG 130 1.0042731E-5 25.571589 4 CGCAGAG 11015 0.0 25.566614 8 GGTAAGG 1440 0.0 24.73555 3 CCGTAAA 245 2.9831426E-10 23.263632 2 GCAGAGT 12360 0.0 22.592375 9 GTACATG 7355 0.0 22.217617 1 GAGTACT 8015 0.0 22.130947 12-13 >>END_MODULE