FastQCFastQC Report
Thu 26 May 2016
SRR937927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526289
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125100.8196350756639141No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99990.6551183950090711No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68520.44893201746196165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45470.2979121254231669No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42410.27786349767311436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.15291992538765595No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.14571290234025142No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.1286126022005007No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17450.11432959288837172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67650.035.6923831
GTATCAA101850.033.3120541
TGACGCG404.4750172E-429.71050694-95
TATCAAC124350.027.1753732
ATCAACG124350.027.1753733
TCAACGC125650.026.8563844
CAACGCA126700.026.633825
AACGCAG129750.026.076736
GTACATG88550.024.7352871
TACATGG87350.024.64832
ACGCAGA142950.023.6293647
CGCAGAG143500.023.6050118
ACATGGG88750.023.5097433
GAGTACT79650.023.08070612-13
GCAGAGT151350.021.9719189
AGTACTT83550.021.46319612-13
GTACTTT86750.021.13830414-15
GTACCGT1353.785713E-421.1201976
TACCTGG11100.020.5527522
CATGGGG34200.020.2898314
Produced by FastQC (version 0.11.5)