##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937927_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1526289 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07563639651468 33.0 31.0 34.0 31.0 34.0 2 32.225724617028625 34.0 31.0 34.0 31.0 34.0 3 32.312875870821316 34.0 31.0 34.0 30.0 34.0 4 35.83756614900586 37.0 35.0 37.0 35.0 37.0 5 35.8601909599034 37.0 35.0 37.0 35.0 37.0 6 35.909257027994045 37.0 36.0 37.0 35.0 37.0 7 35.84895783170815 37.0 36.0 37.0 35.0 37.0 8 35.82927741731743 37.0 36.0 37.0 35.0 37.0 9 37.49231567547168 39.0 38.0 39.0 35.0 39.0 10-11 37.40412595517625 39.0 37.0 39.0 35.0 39.0 12-13 37.28123114298799 39.0 37.0 39.0 34.0 39.0 14-15 38.458737827501864 40.0 38.0 41.0 34.0 41.0 16-17 38.28889188089543 40.0 38.0 41.0 34.0 41.0 18-19 38.49310418931146 40.0 38.0 41.0 34.0 41.0 20-21 38.487388037258995 40.0 38.0 41.0 34.0 41.0 22-23 38.36627237698758 40.0 38.0 41.0 34.0 41.0 24-25 38.235388252159325 40.0 38.0 41.0 34.0 41.0 26-27 38.027783401439706 40.0 38.0 41.0 33.5 41.0 28-29 37.81860086785662 40.0 38.0 41.0 33.0 41.0 30-31 37.54753293773329 40.0 37.0 41.0 33.0 41.0 32-33 37.20582668157865 39.5 36.0 41.0 32.0 41.0 34-35 36.91129268441298 39.0 35.5 41.0 31.0 41.0 36-37 36.60304634312375 39.0 35.0 41.0 30.5 41.0 38-39 36.280178917623076 39.0 35.0 40.0 30.0 41.0 40-41 36.11364066700344 39.0 35.0 40.0 30.0 41.0 42-43 35.84942825375797 39.0 35.0 40.0 29.0 41.0 44-45 35.469707571763934 38.5 35.0 40.0 27.5 41.0 46-47 34.978674418802726 38.0 34.5 40.0 25.0 41.0 48-49 34.48271166207711 38.0 34.0 40.0 23.5 41.0 50-51 33.65354890194452 37.5 33.0 39.5 22.5 40.5 52-53 33.63945360282358 38.0 33.0 39.5 22.0 40.5 54-55 33.63617997640027 38.0 33.0 40.0 19.5 41.0 56-57 33.19402812966614 37.5 33.0 40.0 16.5 41.0 58-59 32.75456483012064 37.0 32.0 40.0 11.0 41.0 60-61 32.21872102858633 36.0 31.5 39.5 7.5 41.0 62-63 31.61907934866857 35.5 31.0 39.0 2.0 41.0 64-65 31.031433431021256 35.0 30.0 39.0 2.0 40.0 66-67 30.370485209550747 35.0 29.0 38.5 2.0 40.0 68-69 29.687201440880465 35.0 28.5 38.0 2.0 40.0 70-71 28.88921560726704 34.0 27.0 37.0 2.0 39.5 72-73 28.102357744830762 34.0 26.0 36.5 2.0 39.0 74-75 27.29827411453532 33.5 24.5 36.0 2.0 39.0 76-77 26.47091212738872 33.0 21.5 35.0 2.0 38.0 78-79 25.659226725738048 32.0 19.0 35.0 2.0 37.0 80-81 24.881435298295408 32.0 11.0 35.0 2.0 36.5 82-83 24.06735978572865 31.0 4.5 35.0 2.0 36.0 84-85 23.273237244060596 31.0 2.0 34.5 2.0 35.5 86-87 22.498003982207827 30.0 2.0 34.0 2.0 35.0 88-89 21.685500583441275 29.5 2.0 34.0 2.0 35.0 90-91 20.83164164846893 29.0 2.0 34.0 2.0 35.0 92-93 19.930784078244685 27.0 2.0 34.0 2.0 35.0 94-95 18.99671392508234 25.5 2.0 34.0 2.0 35.0 96-97 17.907552567043332 24.0 2.0 33.0 2.0 35.0 98-99 16.598952754032823 10.0 2.0 33.0 2.0 35.0 100-101 13.470351945142761 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4906289510601525 1101 2 0.49223889101866547 1101 3 0.4043297129108012 1101 4 0.30635837303648117 1101 5 0.3007473325218939 1101 6 0.374896207677331 1101 7 0.31751918287687175 1101 8 0.29464706237993 1101 9 0.4597557159673116 1101 10-11 0.45171912370609135 1101 12-13 0.5427908819483775 1101 14-15 0.6966921111495807 1101 16-17 0.6554962593021045 1101 18-19 0.7166623578433757 1101 20-21 0.6437468657770964 1101 22-23 0.666034087067672 1101 24-25 0.7130922695446316 1101 26-27 0.7951768897475588 1101 28-29 0.8314528061436377 1101 30-31 0.8366663778002064 1101 32-33 0.8096596905747973 1101 34-35 0.8210974739480932 1101 36-37 0.9846596830234517 1101 38-39 1.112937295400009 1101 40-41 1.2003460158426265 1101 42-43 1.3069936234402562 1101 44-45 1.3809792910605267 1101 46-47 1.53717149356941 1101 48-49 1.6431719840284984 1101 50-51 1.7559316605659347 1101 52-53 1.6747622011821122 1101 54-55 1.7752858383173802 1101 56-57 1.7134218935454797 1101 58-59 1.9722554687671874 1101 60-61 2.158398256627315 1101 62-63 2.3040110990358365 1101 64-65 2.2738268402985113 1101 66-67 2.4178113269866905 1101 68-69 2.6345714349502636 1101 70-71 3.027202636672648 1101 72-73 3.449583669263614 1101 74-75 3.52562058299873 1101 76-77 3.706933334381315 1101 78-79 3.929459335100187 1101 80-81 4.017918271402561 1101 82-83 4.379744983154591 1101 84-85 4.575449067212272 1101 86-87 4.306895457716703 1101 88-89 4.399010157499756 1101 90-91 4.537710695209427 1101 92-93 4.71329758122198 1101 94-95 4.9720992859475395 1101 96-97 4.805930540322304 1101 98-99 4.861293397908913 1101 100-101 4.1539511670048785 1102 1 0.3416840001732879 1102 2 0.3459085963398465 1102 3 0.3006621275811412 1102 4 0.252044190293212 1102 5 0.19983290951292076 1102 6 0.23553927177174216 1102 7 0.3123774967769464 1102 8 0.2718840067654895 1102 9 0.4382194287791279 1102 10-11 0.33126685884848683 1102 12-13 0.3832473867890229 1102 14-15 0.5506925351478813 1102 16-17 0.6257513649483499 1102 18-19 0.6029530793471594 1102 20-21 0.5069339196955482 1102 22-23 0.6116803311493584 1102 24-25 0.5956419126793833 1102 26-27 0.5846179186501104 1102 28-29 0.6200119185738586 1102 30-31 0.6807056683097059 1102 32-33 0.7257235729859346 1102 34-35 0.8670164902720288 1102 36-37 0.8219291339456447 1102 38-39 1.096175229114941 1102 40-41 1.0312809321029661 1102 42-43 1.1694528136034918 1102 44-45 1.3237974664544936 1102 46-47 1.322146407003089 1102 48-49 1.2842588330144338 1102 50-51 1.5540999878926272 1102 52-53 1.4750646533056369 1102 54-55 1.5288877039099233 1102 56-57 1.8069658127031332 1102 58-59 1.8443973668662608 1102 60-61 2.0612305652980822 1102 62-63 2.1424931851074867 1102 64-65 2.1471545203211235 1102 66-67 2.28580652167258 1102 68-69 2.495114152779397 1102 70-71 2.7573687026750484 1102 72-73 3.000182213536103 1102 74-75 3.10056934987033 1102 76-77 3.0972280132025993 1102 78-79 3.3389936436296175 1102 80-81 3.5706212605906025 1102 82-83 3.755032948669392 1102 84-85 3.947090647312617 1102 86-87 4.021615265504142 1102 88-89 4.066742473237163 1102 90-91 4.233952656908244 1102 92-93 4.418802492535669 1102 94-95 3.989811108433585 1102 96-97 4.098141691477704 1102 98-99 4.218899220818955 1102 100-101 3.7739384470557606 1103 1 0.49137009399485976 1103 2 0.35714455139602563 1103 3 0.35967110017834614 1103 4 0.3136883581050256 1103 5 0.33658552988853074 1103 6 0.35060137938901903 1103 7 0.3433343043645891 1103 8 0.34930431770774817 1103 9 0.3616397936108555 1103 10-11 0.3932842112586954 1103 12-13 0.3991299616280273 1103 14-15 0.61294295952748 1103 16-17 0.6852722875361152 1103 18-19 0.62927943399378 1103 20-21 0.5867725207248426 1103 22-23 0.7062105496711695 1103 24-25 0.5931818582510218 1103 26-27 0.7333959748568262 1103 28-29 0.7659648191747195 1103 30-31 0.8418109413946055 1103 32-33 0.8110225248356855 1103 34-35 1.057401261107053 1103 36-37 1.1014982876114772 1103 38-39 1.0946259067750574 1103 40-41 1.312891149546985 1103 42-43 1.2312667332542944 1103 44-45 1.4054790874498266 1103 46-47 1.52556704128218 1103 48-49 1.420712366816609 1103 50-51 1.5876623514848944 1103 52-53 1.622693516507212 1103 54-55 1.8350917294535307 1103 56-57 2.082110640660993 1103 58-59 2.1272995844180613 1103 60-61 2.2016193778435564 1103 62-63 2.2330962083069075 1103 64-65 2.3148706844684597 1103 66-67 2.441212983020346 1103 68-69 2.590045847872762 1103 70-71 2.766962376050955 1103 72-73 3.142618826043851 1103 74-75 3.4046622553212487 1103 76-77 3.703359287958076 1103 78-79 3.782865063347778 1103 80-81 3.996932795632233 1103 82-83 4.085724350604028 1103 84-85 4.201470514205621 1103 86-87 4.447169653454456 1103 88-89 4.537317473910779 1103 90-91 4.540688840994683 1103 92-93 4.647124104908691 1103 94-95 4.615044854021381 1103 96-97 4.835543679999244 1103 98-99 5.167498094528973 1103 100-101 4.290958089768557 1104 1 0.5057712938830079 1104 2 0.4490173429154609 1104 3 0.42937138235542704 1104 4 0.34340084253444303 1104 5 0.31383087142448574 1104 6 0.24530356816803334 1104 7 0.34877193306022036 1104 8 0.25754592230392603 1104 9 0.2818345212209934 1104 10-11 0.4356527192791333 1104 12-13 0.501154772874429 1104 14-15 0.6644059587394153 1104 16-17 0.6651182341004258 1104 18-19 0.7585584842518003 1104 20-21 0.6244482458691039 1104 22-23 0.6698783433514208 1104 24-25 0.7818795220281274 1104 26-27 0.888204424916573 1104 28-29 0.9664618067436734 1104 30-31 1.0003639576388608 1104 32-33 1.1078982398114121 1104 34-35 1.1230176019566258 1104 36-37 1.0272691903239348 1104 38-39 1.3009964993469296 1104 40-41 1.3411114002015907 1104 42-43 1.4613732487486075 1104 44-45 1.4763576496177606 1104 46-47 1.750580387495667 1104 48-49 1.8475242741811755 1104 50-51 1.9283644894211918 1104 52-53 1.9268003370578342 1104 54-55 2.0940324214229165 1104 56-57 2.367789518053776 1104 58-59 2.4431281936155145 1104 60-61 2.4294443263653065 1104 62-63 2.605680543665475 1104 64-65 2.8763230067096046 1104 66-67 3.0202581567753377 1104 68-69 3.1970596008279877 1104 70-71 3.4176233313856272 1104 72-73 3.6940653013724223 1104 74-75 3.8644965080604017 1104 76-77 3.981414299779008 1104 78-79 4.329144647454346 1104 80-81 4.381682522352623 1104 82-83 4.479804987154857 1104 84-85 4.551260787993659 1104 86-87 4.677113472251005 1104 88-89 4.990037559326545 1104 90-91 5.043256398256297 1104 92-93 5.048379920044567 1104 94-95 5.127793665572849 1104 96-97 5.191737594125893 1104 98-99 5.277995844738701 1104 100-101 4.686564007860936 1105 1 0.4030627071174777 1105 2 0.3391117645107755 1105 3 0.2794611697620226 1105 4 0.23191770905049935 1105 5 0.3110489100041036 1105 6 0.2469747724778273 1105 7 0.24380900657003934 1105 8 0.14894853675873776 1105 9 0.396546622246305 1105 10-11 0.3500302898056944 1105 12-13 0.3873818191583922 1105 14-15 0.5252159280379161 1105 16-17 0.48202554067131587 1105 18-19 0.48749826876203173 1105 20-21 0.6325655584258243 1105 22-23 0.6063923714268782 1105 24-25 0.5039614313138827 1105 26-27 0.5858274722818422 1105 28-29 0.6718538784191352 1105 30-31 0.6836712236606033 1105 32-33 0.8084912711501104 1105 34-35 0.8504664810622344 1105 36-37 0.8990658073661564 1105 38-39 0.8527970747587048 1105 40-41 0.9384223639197486 1105 42-43 0.872617914604298 1105 44-45 1.0149652689963986 1105 46-47 1.22893172658312 1105 48-49 1.4007390012467695 1105 50-51 1.5668678395534599 1105 52-53 1.5907543137710363 1105 54-55 1.7676103047572198 1105 56-57 2.03637116666642 1105 58-59 2.2475977720974285 1105 60-61 2.3843939778356926 1105 62-63 2.480030791787666 1105 64-65 2.4936732162135513 1105 66-67 2.811699872197533 1105 68-69 2.572352133991405 1105 70-71 2.8436484042775554 1105 72-73 3.131470203957914 1105 74-75 3.2480842390457454 1105 76-77 3.6873658531976865 1105 78-79 3.7855545511018356 1105 80-81 3.952674431831472 1105 82-83 4.213202040583191 1105 84-85 4.06924481935167 1105 86-87 4.352659196246453 1105 88-89 4.400085783852987 1105 90-91 4.577474330635759 1105 92-93 4.577438214276935 1105 94-95 4.659305183462749 1105 96-97 4.605600093884529 1105 98-99 4.49224452689128 1105 100-101 4.011392398059629 1106 1 0.46328863889316096 1106 2 0.3053173088711105 1106 3 0.2680293305375798 1106 4 0.31820567448448145 1106 5 0.24960209338191675 1106 6 0.29267217458011174 1106 7 0.2593432911950586 1106 8 0.31258976908086566 1106 9 0.33449526338785773 1106 10-11 0.2364580442852926 1106 12-13 0.2678206569123276 1106 14-15 0.5077420704048947 1106 16-17 0.3593036936404772 1106 18-19 0.434978661986797 1106 20-21 0.462275042295623 1106 22-23 0.4064854128371138 1106 24-25 0.4504131496298811 1106 26-27 0.596019364517197 1106 28-29 0.5218684019563398 1106 30-31 0.6258784382277085 1106 32-33 0.5299355007672375 1106 34-35 0.5931466183096603 1106 36-37 0.8980051352894804 1106 38-39 0.7788559313932311 1106 40-41 0.9152071704843863 1106 42-43 0.6165579687044698 1106 44-45 0.8473718191116788 1106 46-47 1.063050011716463 1106 48-49 0.9716218053787173 1106 50-51 1.2080810995429303 1106 52-53 1.120455174290619 1106 54-55 1.2803043301371062 1106 56-57 1.1116679914580985 1106 58-59 1.6683151440633637 1106 60-61 1.787342028958541 1106 62-63 1.4825656028909044 1106 64-65 1.8024408940814993 1106 66-67 1.910119983665684 1106 68-69 1.7188515076638602 1106 70-71 2.1347711190897485 1106 72-73 2.4900375023984473 1106 74-75 2.681447073314029 1106 76-77 2.4722364121723253 1106 78-79 2.684017098530262 1106 80-81 2.982338756577761 1106 82-83 2.8977980139324977 1106 84-85 2.9160119707413905 1106 86-87 3.215677504980544 1106 88-89 3.3057261356856777 1106 90-91 3.4654145753573573 1106 92-93 3.5629872947554837 1106 94-95 3.2515777539472914 1106 96-97 3.2852222096008106 1106 98-99 3.1123019856353586 1106 100-101 2.6704258112724144 1107 1 0.5806892559492525 1107 2 0.4656493524934149 1107 3 0.44042723601042155 1107 4 0.32208164347672863 1107 5 0.35157995410224174 1107 6 0.3114860050218482 1107 7 0.32383385534109266 1107 8 0.27237721741267507 1107 9 0.4037285917081803 1107 10-11 0.4211360038986598 1107 12-13 0.43618969655613427 1107 14-15 0.532154750588461 1107 16-17 0.6704925064227396 1107 18-19 0.5818097554156552 1107 20-21 0.5824421578546364 1107 22-23 0.4842932686811494 1107 24-25 0.6216726458678394 1107 26-27 0.6615487612696782 1107 28-29 0.6856443841647106 1107 30-31 0.661796434481623 1107 32-33 0.8275761954794163 1107 34-35 0.9923012423566178 1107 36-37 1.1996375539761601 1107 38-39 1.0918681130100651 1107 40-41 1.2749447165699124 1107 42-43 1.3353503799172088 1107 44-45 1.4303332781713252 1107 46-47 1.5420220254429964 1107 48-49 1.7032320300983343 1107 50-51 1.827178392972698 1107 52-53 1.936538736627412 1107 54-55 1.8767453201612128 1107 56-57 2.051260752670153 1107 58-59 1.9727461900964798 1107 60-61 2.101051930566804 1107 62-63 2.144867531988698 1107 64-65 2.33831217818706 1107 66-67 2.6551835349738546 1107 68-69 2.581496144425639 1107 70-71 2.8191186757025903 1107 72-73 3.1713617576245134 1107 74-75 3.2989764336082494 1107 76-77 3.291176935074951 1107 78-79 3.4977627748082405 1107 80-81 3.7026013472107238 1107 82-83 3.873898255924214 1107 84-85 4.070669969456237 1107 86-87 4.037559070316025 1107 88-89 4.3180918667421935 1107 90-91 4.510909701343444 1107 92-93 4.326735477538978 1107 94-95 4.320715379329531 1107 96-97 4.391976558387892 1107 98-99 4.275607656262306 1107 100-101 3.7312379646340954 1108 1 0.34796364186154705 1108 2 0.3198635422860008 1108 3 0.28979466775735574 1108 4 0.14630495578693825 1108 5 0.2835422182169083 1108 6 0.15063314094543756 1108 7 0.27195902897172886 1108 8 0.2875240572629849 1108 9 0.41598583324726235 1108 10-11 0.4287348976612222 1108 12-13 0.35911619828691244 1108 14-15 0.503646402379367 1108 16-17 0.5135647761801039 1108 18-19 0.5145045125154084 1108 20-21 0.5338786900290629 1108 22-23 0.5018889613916855 1108 24-25 0.43879548647948496 1108 26-27 0.5189459791421243 1108 28-29 0.566147574162045 1108 30-31 0.6068717168807396 1108 32-33 0.7138936687054596 1108 34-35 0.8950142541043817 1108 36-37 0.907083027948147 1108 38-39 0.9351874309264403 1108 40-41 1.0530024662399526 1108 42-43 0.9803690911717453 1108 44-45 1.0553696865332753 1108 46-47 1.1106146584092258 1108 48-49 0.9838724904346066 1108 50-51 1.1271006256728384 1108 52-53 1.1336399100581005 1108 54-55 1.3097613840592075 1108 56-57 1.5465596280723233 1108 58-59 1.442862494986592 1108 60-61 1.5550118145599114 1108 62-63 1.7443128271631885 1108 64-65 1.8706754870174507 1108 66-67 1.782626019374728 1108 68-69 1.742847670885265 1108 70-71 1.9545126021629855 1108 72-73 2.297026120110491 1108 74-75 2.2703839736718656 1108 76-77 2.6063325225449 1108 78-79 2.5868024880401173 1108 80-81 2.6595837260441755 1108 82-83 2.7970513786802336 1108 84-85 3.14730848993576 1108 86-87 3.32808650371636 1108 88-89 3.4364389895924177 1108 90-91 3.2986965343494603 1108 92-93 3.7658977055096408 1108 94-95 3.611469914393549 1108 96-97 3.9117043632256134 1108 98-99 3.7880057248647887 1108 100-101 2.5476910355772926 1109 1 0.28362788481380363 1109 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4.533319373570965 2311 92-93 4.577828534339385 2311 94-95 4.575971850129417 2311 96-97 4.827301889356814 2311 98-99 4.633637969514229 2311 100-101 4.5361289320174745 2312 1 -0.20206702697810286 2312 2 -0.15105406292268242 2312 3 -0.0038841526171111695 2312 4 0.026084722768494828 2312 5 -0.19061220384134714 2312 6 -0.08803071724314293 2312 7 6.191000845916506E-4 2312 8 -0.09537559655945671 2312 9 -0.05171275760452687 2312 10-11 -0.05423560199000832 2312 12-13 0.043907488336451195 2312 14-15 0.056557029010924964 2312 16-17 0.12335193928816324 2312 18-19 0.024579914163915362 2312 20-21 0.05658576943303473 2312 22-23 0.026378110772405705 2312 24-25 0.14368902757330204 2312 26-27 0.0446766500856981 2312 28-29 0.13619593220577997 2312 30-31 0.01954907421399099 2312 32-33 0.0841533504292542 2312 34-35 0.27559331254672514 2312 36-37 0.42710597697961106 2312 38-39 0.5028573354888906 2312 40-41 0.7051573365973454 2312 42-43 0.6928705679694289 2312 44-45 0.8124482003811906 2312 46-47 0.8889436840788036 2312 48-49 0.9138898802331283 2312 50-51 1.0025119037137813 2312 52-53 1.065716926878224 2312 54-55 1.3386406685932144 2312 56-57 1.5350223990141316 2312 58-59 1.591766994323855 2312 60-61 1.8733474918881186 2312 62-63 1.811057811310878 2312 64-65 1.9145155413312338 2312 66-67 2.0573522655896817 2312 68-69 2.2531674522059184 2312 70-71 2.61326390295876 2312 72-73 2.9110713157841595 2312 74-75 2.592019990380315 2312 76-77 2.818951940856543 2312 78-79 3.3464581150034505 2312 80-81 3.4994948106853165 2312 82-83 3.812212628803639 2312 84-85 4.152590772733209 2312 86-87 4.3033408366003165 2312 88-89 4.527071075186804 2312 90-91 4.742767778306728 2312 92-93 5.012348738135135 2312 94-95 5.091361998919783 2312 96-97 5.0591330763805225 2312 98-99 5.1099848441592925 2312 100-101 4.429758827469387 2313 1 -0.47296863265227884 2313 2 -0.4150745338665409 2313 3 -0.4041805627036581 2313 4 -0.15796729309394664 2313 5 -0.21181374053139024 2313 6 -0.20988714678608744 2313 7 -0.26923470310588726 2313 8 -0.1931762545820206 2313 9 -0.08846115329959758 2313 10-11 -0.14068533811592232 2313 12-13 -0.08859209110809729 2313 14-15 -0.23535800444442856 2313 16-17 -0.20025797825689295 2313 18-19 -0.231764604857581 2313 20-21 -0.16109764164328766 2313 22-23 -0.06297606602780093 2313 24-25 0.02144252470374397 2313 26-27 -0.01616794261449428 2313 28-29 0.030717692200980196 2313 30-31 0.10450567550004308 2313 32-33 0.00687009296977692 2313 34-35 0.3180845721657093 2313 36-37 0.30846565555788175 2313 38-39 0.3341511922496707 2313 40-41 0.44421718978762925 2313 42-43 0.4476388190977545 2313 44-45 0.44356369599111645 2313 46-47 0.29950425514745405 2313 48-49 0.3732409142779076 2313 50-51 0.4983034151444201 2313 52-53 0.5206731565106253 2313 54-55 0.6095086950340871 2313 56-57 0.8639009960672368 2313 58-59 0.9524930490703909 2313 60-61 1.2334058741123783 2313 62-63 1.3512510125267703 2313 64-65 1.5748451329164723 2313 66-67 1.6296136346052386 2313 68-69 1.7836815209266383 2313 70-71 2.5120234926009672 2313 72-73 2.6184644849204766 2313 74-75 2.670806652056825 2313 76-77 2.7814310597896217 2313 78-79 3.029562251052159 2313 80-81 3.137053606944786 2313 82-83 3.6034156521082785 2313 84-85 3.683360597847347 2313 86-87 3.930459588417836 2313 88-89 3.9022003389343993 2313 90-91 4.056368313227175 2313 92-93 4.225193642502784 2313 94-95 4.154301218551442 2313 96-97 4.409399851978659 2313 98-99 4.291789333474576 2313 100-101 3.7638679606296304 2314 1 -0.40626764084814226 2314 2 -0.2799132812822478 2314 3 -0.25612392242232573 2314 4 -0.18821219893389696 2314 5 -0.2132708679802633 2314 6 -0.24817668967734363 2314 7 -0.2922535756963285 2314 8 -0.22153697669075711 2314 9 -0.07401352539592665 2314 10-11 -0.17458748801299606 2314 12-13 -0.17010626084822889 2314 14-15 0.020627996225805134 2314 16-17 0.0666415772811888 2314 18-19 0.022408546649749894 2314 20-21 -0.04492517558190201 2314 22-23 -0.06200591589865212 2314 24-25 -0.08797899633313477 2314 26-27 -0.03581015378320984 2314 28-29 -0.0474778348996594 2314 30-31 0.006871795574824091 2314 32-33 0.10146480324204532 2314 34-35 0.23292568154823812 2314 36-37 0.3820291913556275 2314 38-39 0.39502177200314037 2314 40-41 0.4118530131835385 2314 42-43 0.5972606983292863 2314 44-45 0.5685139599448732 2314 46-47 0.7637480405591219 2314 48-49 1.0043752512199262 2314 50-51 0.9169620335808872 2314 52-53 1.051242872418328 2314 54-55 1.351628999356869 2314 56-57 1.677980918049414 2314 58-59 1.6259950469138715 2314 60-61 1.7485833339105952 2314 62-63 1.933171947827585 2314 64-65 2.132892448977117 2314 66-67 2.352890199360697 2314 68-69 2.573312867047985 2314 70-71 2.94656784383843 2314 72-73 3.1012608063394573 2314 74-75 3.235997411788997 2314 76-77 3.572446455057637 2314 78-79 3.7739346669574196 2314 80-81 3.9866701915661302 2314 82-83 4.134731052036816 2314 84-85 4.572783615324951 2314 86-87 4.677744991050744 2314 88-89 4.8648004173707236 2314 90-91 4.861138915522371 2314 92-93 5.045648497073788 2314 94-95 5.086543365487341 2314 96-97 5.10932528357657 2314 98-99 5.017279964709417 2314 100-101 4.372842610226305 2315 1 -0.3554531799054388 2315 2 -0.2595036173054197 2315 3 -0.11387228486944423 2315 4 -0.13592797546518653 2315 5 -0.16933035893205073 2315 6 -0.18563653372110878 2315 7 -0.17633209348272771 2315 8 -0.13056936381318707 2315 9 -0.18514994441004973 2315 10-11 -0.16117765764796133 2315 12-13 -0.14394607698613981 2315 14-15 -0.19541665688593213 2315 16-17 -0.08570240884440494 2315 18-19 -0.2144142237326534 2315 20-21 -0.17422205764132315 2315 22-23 -0.013388776417350812 2315 24-25 0.04895242492753482 2315 26-27 0.02933893192292203 2315 28-29 0.022573782836197154 2315 30-31 0.0066441802419277 2315 32-33 0.11240108902097035 2315 34-35 0.16088659714454678 2315 36-37 -0.03914719821961654 2315 38-39 0.14051776154872897 2315 40-41 0.18726055524329155 2315 42-43 0.3147738676133969 2315 44-45 0.4048083698618612 2315 46-47 0.5340416378535409 2315 48-49 0.7637824168808862 2315 50-51 0.7378739469144406 2315 52-53 0.9316767405827804 2315 54-55 1.0717009326570022 2315 56-57 1.272622781447815 2315 58-59 1.5192366008426603 2315 60-61 1.5347911534086762 2315 62-63 1.7511920091357247 2315 64-65 2.0909300589444584 2315 66-67 1.919254168274147 2315 68-69 2.2301208245174173 2315 70-71 2.6417753336759766 2315 72-73 2.9155326148795986 2315 74-75 3.1570856218444554 2315 76-77 3.197122013626597 2315 78-79 3.449903286265556 2315 80-81 3.6170303690718164 2315 82-83 4.011875948748372 2315 84-85 4.337158279198956 2315 86-87 4.399792239070756 2315 88-89 4.565358659164573 2315 90-91 4.743060306752156 2315 92-93 4.825894695539407 2315 94-95 5.0992247021517585 2315 96-97 5.213677488158044 2315 98-99 5.267264541083161 2315 100-101 5.0068812446963324 2316 1 -0.6444632990913313 2316 2 -0.5479495792795035 2316 3 -0.48756313900837966 2316 4 -0.4514510254047721 2316 5 -0.41783976708319415 2316 6 -0.4065526316214374 2316 7 -0.39990366340073535 2316 8 -0.3514190902768277 2316 9 -0.5037107166785901 2316 10-11 -0.39903475748657513 2316 12-13 -0.43389584364138045 2316 14-15 -0.43971086425734285 2316 16-17 -0.29994326095532386 2316 18-19 -0.39171015307410784 2316 20-21 -0.5232731304618525 2316 22-23 -0.4032045096435013 2316 24-25 -0.5732856322084388 2316 26-27 -0.37092415707748927 2316 28-29 -0.4613541894057889 2316 30-31 -0.5057383945519192 2316 32-33 -0.31198033533464553 2316 34-35 -0.1685366596526876 2316 36-37 0.04534732798050811 2316 38-39 -0.1960610121283608 2316 40-41 -0.13412837768836994 2316 42-43 0.2290164404652657 2316 44-45 0.4293762487905255 2316 46-47 0.47689275811735143 2316 48-49 0.7587203657330903 2316 50-51 0.7903623841498018 2316 52-53 0.7099346870259424 2316 54-55 1.0642467443120793 2316 56-57 1.260671313717232 2316 58-59 1.4460006368651435 2316 60-61 1.3635878761346234 2316 62-63 1.5016685929241298 2316 64-65 1.8469037955211434 2316 66-67 1.988857525193918 2316 68-69 2.1861826261577733 2316 70-71 2.47685306814844 2316 72-73 3.0073132499067547 2316 74-75 3.052654160800188 2316 76-77 3.4341411740770873 2316 78-79 3.737228615695269 2316 80-81 3.8588614586818615 2316 82-83 3.901009531097504 2316 84-85 4.180851394883138 2316 86-87 4.654824791802248 2316 88-89 5.023666335021225 2316 90-91 5.277319337262121 2316 92-93 5.187572012363457 2316 94-95 5.319794808210855 2316 96-97 5.585886661428052 2316 98-99 5.882235540452633 2316 100-101 5.560126807950153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3555.0 3 1028.0 4 1082.0 5 1188.0 6 1450.0 7 1905.0 8 2317.0 9 3130.0 10 3984.0 11 5176.0 12 7184.0 13 9471.0 14 10859.0 15 13904.0 16 17180.0 17 22178.0 18 22001.0 19 19289.0 20 20305.0 21 22232.0 22 24727.0 23 27837.0 24 31028.0 25 34640.0 26 38454.0 27 42719.0 28 46784.0 29 52075.0 30 57655.0 31 64955.0 32 75593.0 33 89166.0 34 111884.0 35 150747.0 36 170909.0 37 190887.0 38 117247.0 39 9562.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.40857502737865 18.437591467726076 13.838306437025807 22.315527067869464 2 15.638790770218817 20.233038212762054 40.64491818104369 23.48325283597544 3 19.579049068555445 25.511768495996108 29.333047551815927 25.576134883632516 4 12.557824627359684 16.425143570242255 36.93382988122438 34.083201921173675 5 13.251923689603226 38.03254637635746 33.84008957333358 14.87544036070573 6 31.525876163093315 35.74493693041696 17.657844398206965 15.07134250828276 7 28.248975622032713 33.60792080765289 20.425231550054924 17.717872020259485 8 26.630816973224146 34.43064853864984 19.68993619803187 19.248598290094147 9 27.12394954403231 16.699415244539466 20.26405097505122 35.91258423637701 10-11 25.104319793005413 26.85293383238183 26.644654704270508 21.398091670342247 12-13 26.332899912573094 24.51394177394049 27.373732342582407 21.779425970904008 14-15 23.012719838183237 25.51500779691774 26.62148528415743 24.850787080741597 16-17 21.557873599433236 28.788047098824233 27.353933580211233 22.300145721531297 18-19 21.448880066504294 26.939875150442372 30.361687549744694 21.24955723330864 20-21 22.394925473795148 25.99750874740331 30.544356061015698 21.063209717785845 22-23 21.9617105257553 26.139593041220476 30.352962147308997 21.54573428571522 24-25 21.53497592935563 26.380403686779125 30.65520854397264 21.429411839892605 26-27 21.372064001426217 26.534099399888316 30.56328962926259 21.530546969422883 28-29 21.294893304671525 26.91697687830965 30.54009594715042 21.2480338698684 30-31 21.440839516416837 26.701613746288267 30.40799011362061 21.449556623674287 32-33 21.08428918135803 26.73581367730536 30.384333814179342 21.795563327157268 34-35 21.699751080421077 26.743944464324805 30.253690865215095 21.302613590039023 36-37 21.839537733187267 26.47849255350769 30.146177165794274 21.535792547510773 38-39 21.530384365367276 26.687725418859692 30.255732454025036 21.526157761747992 40-41 21.695452902830112 26.70401882438665 30.259277618749163 21.341250654034074 42-43 21.42553616015658 26.791446075355818 29.952005315487483 21.83101244900012 44-45 21.525760131261148 27.035720697875266 29.858438207268378 21.580080963595215 46-47 21.785555959296374 27.156896120691492 29.292306422023763 21.765241497988377 48-49 21.82483892264519 27.119676420044524 29.253580725433757 21.801903931876524 50-51 21.92532833770549 27.462225642628486 29.060512032196378 21.55193398746965 52-53 21.876204975087216 27.455855937314595 29.01349047928401 21.654448608314176 54-55 21.574723492332545 27.471467973072095 29.115657044987188 21.838151489608173 56-57 21.84692631604467 27.66192703516343 28.72376567932807 21.767380969463826 58-59 21.8280760182632 27.243710599621224 28.947441700625426 21.980771681490147 60-61 21.66873552576704 27.349596564285168 29.152075722536345 21.829592187411443 62-63 21.78796170347714 27.34697902497451 29.156183085265585 21.708876186282758 64-65 21.574293304712675 27.320538563679484 29.162370783605105 21.942797348002728 66-67 21.572708384794662 27.5060328509455 28.925218856696937 21.9960399075629 68-69 21.77020067087951 27.388873909837173 29.000124819364103 21.84080059991921 70-71 21.72340491060868 27.316464870255952 28.746568740199326 22.213561478936043 72-73 21.728045019492566 27.417142254296834 28.625160499562774 22.229652226647826 74-75 21.71500030632728 27.672918473369673 28.43962125498519 22.172459965317852 76-77 21.86429927754357 27.271190460901778 28.71426867830966 22.150241583244988 78-79 21.87438572765228 27.323358205630203 28.607957300372956 22.19429876634456 80-81 21.929086546337103 27.57371201369204 28.321316102957656 22.175885337013202 82-83 21.987252532096374 27.532997358638678 28.231801570925235 22.24794853833971 84-85 22.09427654873635 27.311867551821113 28.19644322020794 22.397412679234602 86-87 22.16693900602903 27.308354550469705 28.20497784813029 22.319728595370975 88-89 22.253186356907364 27.194898421255093 28.182768999691344 22.369146222146206 90-91 22.151726406627976 27.308190966480378 28.169551246660834 22.370531380230815 92-93 22.226055515256835 27.140107843310705 28.13053902652704 22.503297614905414 94-95 22.081161722504344 27.027421112611826 28.17713791031239 22.714279254571444 96-97 22.350417114864594 26.715272455740212 27.99736700439739 22.936943424997796 98-99 22.196505731821272 26.784354559048833 27.80749153693018 23.211648172199716 100-101 23.149192415120044 25.85071805546036 27.965678421474994 23.034411107944607 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4452.0 1 2858.0 2 2858.5 3 3622.0 4 2042.5 5 1593.0 6 2788.5 7 5089.0 8 7313.0 9 7616.5 10 5442.0 11 3348.0 12 2936.5 13 2914.5 14 2898.0 15 2924.5 16 2878.0 17 2901.5 18 3030.0 19 3208.5 20 3561.5 21 4113.0 22 4823.0 23 5770.0 24 6939.0 25 8323.5 26 9975.5 27 12186.0 28 14124.0 29 15873.0 30 18225.0 31 20893.0 32 23889.0 33 26058.0 34 27336.5 35 29451.0 36 32823.0 37 35607.0 38 37252.5 39 38713.5 40 40120.5 41 41595.0 42 42749.5 43 44350.0 44 46239.0 45 48397.5 46 50580.5 47 52727.0 48 56736.5 49 57483.0 50 55136.0 51 53111.0 52 51010.5 53 50393.0 54 49693.0 55 47122.0 56 43324.0 57 39669.0 58 36648.5 59 32811.0 60 27926.0 61 23953.0 62 20295.0 63 15944.5 64 11879.0 65 9167.5 66 7245.0 67 5616.0 68 4289.0 69 3275.5 70 2567.5 71 1981.0 72 1504.0 73 1177.0 74 901.5 75 675.5 76 498.0 77 338.5 78 261.0 79 169.0 80 86.0 81 56.5 82 43.0 83 33.0 84 25.0 85 14.5 86 7.5 87 7.5 88 6.5 89 4.0 90 6.5 91 6.5 92 1.5 93 1.0 94 1.0 95 2.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3894413181252043 2 0.023521102491074756 3 0.04245591758834663 4 0.03636270719372282 5 0.054707856768934325 6 0.054445783203574155 7 0.031776419799919936 8 0.031055717495179484 9 0.0342661186708415 10-11 0.02961431288569858 12-13 0.02902464736363821 14-15 0.023946972034785022 16-17 0.02840222264590782 18-19 0.02440560077416531 20-21 0.03606787443269263 22-23 0.02361938007808482 24-25 0.044257673350197764 26-27 0.03754203823784356 28-29 0.029417757711678455 30-31 0.03626442960671276 32-33 0.0331523060180608 34-35 0.03243160371332035 36-37 0.03298851003971069 38-39 0.01578993231294991 40-41 0.012022624810897543 42-43 0.011957106419557502 44-45 0.01120364491914703 46-47 0.018508945553561612 48-49 0.015495099551919722 50-51 0.01012259146203635 52-53 0.004225936241432651 54-55 0.004291454632772692 56-57 0.007567374199774749 58-59 0.0033086787626720755 60-61 0.005601822459573515 62-63 0.006093210394623822 64-65 0.007763929373794871 66-67 0.0040621402630825485 68-69 0.0054707856768934325 70-71 0.016117524269650112 72-73 0.012743327115637994 74-75 0.009401889157295899 76-77 0.007010467873384398 78-79 0.006093210394623822 80-81 0.009631203526986043 82-83 0.011924347223887482 84-85 0.005044916133183165 86-87 4.586287393802877E-4 88-89 0.021621069142213567 90-91 0.0178537616401612 92-93 0.012186420789247645 94-95 0.022800400186334304 96-97 0.01772272485748112 98-99 0.016183042660990153 100-101 0.10813810490673784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1526289.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.28121122618929 #Duplication Level Percentage of deduplicated Percentage of total 1 79.44724924473492 48.68623662306308 2 12.549498322104796 15.380969149192241 3 3.684023546714771 6.772842753854487 4 1.5410861555381479 3.77758504861157 5 0.7500496846521891 2.2981976577653738 6 0.44340073285256226 1.6303280380671001 7 0.29555586310632825 1.2678414893310315 8 0.222701154884247 1.091791721022227 9 0.1479642283447917 0.8160684418005557 >10 0.8420994660964397 9.195614355884207 >50 0.04850845356784671 2.0530041104697037 >100 0.024755458472407996 2.9393315813878194 >500 0.0017145869961620154 0.7246682284550079 >1k 0.0010716168726012595 1.4373845205639522 >5k 2.1432337452025193E-4 1.1066048316896546 >10k+ 1.0716168726012596E-4 0.8215314488420614 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12510 0.8196350756639141 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9999 0.6551183950090711 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6852 0.44893201746196165 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4547 0.2979121254231669 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4241 0.27786349767311436 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2334 0.15291992538765595 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2224 0.14571290234025142 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1963 0.1286126022005007 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1745 0.11432959288837172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.551839134004111E-5 0.0 0.0 0.0 0.0 7 6.551839134004111E-5 0.0 0.0 0.0 0.0 8 6.551839134004111E-5 0.0 0.0 0.0 0.0 9 6.551839134004111E-5 0.0 0.0 0.0 0.0 10-11 6.551839134004111E-5 0.0 0.0 0.0 0.0 12-13 6.551839134004111E-5 0.0 0.0 0.0 1.3103678268008221E-4 14-15 6.551839134004111E-5 0.0 0.0 0.0 1.3103678268008221E-4 16-17 6.551839134004111E-5 0.0 0.0 6.551839134004111E-5 1.3103678268008221E-4 18-19 6.551839134004111E-5 0.0 0.0 1.3103678268008221E-4 1.3103678268008221E-4 20-21 6.551839134004111E-5 0.0 0.0 1.6379597835010275E-4 1.3103678268008221E-4 22-23 6.551839134004111E-5 0.0 0.0 3.603511523702261E-4 1.9655517402012332E-4 24-25 6.551839134004111E-5 0.0 0.0 8.84498283090555E-4 1.9655517402012332E-4 26-27 6.551839134004111E-5 0.0 0.0 0.0022276253055613974 1.9655517402012332E-4 28-29 6.551839134004111E-5 0.0 0.0 0.005536304068233473 1.9655517402012332E-4 30-31 6.551839134004111E-5 0.0 0.0 0.016346838639340257 1.9655517402012332E-4 32-33 6.551839134004111E-5 0.0 0.0 0.03708340949846327 2.293143696901439E-4 34-35 6.551839134004111E-5 0.0 0.0 0.06522355857901092 2.6207356536016443E-4 36-37 6.551839134004111E-5 0.0 0.0 0.11560720151950253 2.6207356536016443E-4 38-39 6.551839134004111E-5 0.0 0.0 0.1887257262549884 2.6207356536016443E-4 40-41 6.551839134004111E-5 0.0 0.0 0.26715124068901763 2.6207356536016443E-4 42-43 6.551839134004111E-5 0.0 0.0 0.34253014992573494 2.6207356536016443E-4 44-45 6.551839134004111E-5 0.0 0.0 0.4372697438034343 2.6207356536016443E-4 46-47 6.551839134004111E-5 0.0 0.0 0.549928617712635 2.6207356536016443E-4 48-49 6.551839134004111E-5 0.0 0.0 0.6659289295801778 2.6207356536016443E-4 50-51 6.551839134004111E-5 0.0 0.0 0.7899224851912056 3.275919567002055E-4 52-53 6.551839134004111E-5 0.0 0.0 0.9028106734700965 3.275919567002055E-4 54-55 6.551839134004111E-5 0.0 0.0 1.0300801486481261 3.275919567002055E-4 56-57 6.551839134004111E-5 0.0 0.0 1.1707481348551947 3.275919567002055E-4 58-59 6.551839134004111E-5 0.0 0.0 1.324814632091301 3.275919567002055E-4 60-61 9.827758701006166E-5 0.0 0.0 1.49781594442468 3.275919567002055E-4 62-63 1.3103678268008221E-4 0.0 0.0 1.6608912204700421 3.275919567002055E-4 64-65 1.6379597835010275E-4 0.0 0.0 1.8211492056877825 3.275919567002055E-4 66-67 2.6207356536016443E-4 0.0 0.0 2.005026570983608 3.275919567002055E-4 68-69 2.6207356536016443E-4 0.0 0.0 2.208690490464126 3.275919567002055E-4 70-71 2.6207356536016443E-4 0.0 0.0 2.424049442798841 3.603511523702261E-4 72-73 2.6207356536016443E-4 0.0 0.0 2.635870402001194 3.9311034804024664E-4 74-75 2.6207356536016443E-4 0.0 0.0 2.8457258094633455 3.9311034804024664E-4 76-77 2.6207356536016443E-4 0.0 0.0 3.074384995240089 3.9311034804024664E-4 78-79 2.6207356536016443E-4 0.0 0.0 3.323092808766885 4.586287393802877E-4 80-81 2.6207356536016443E-4 0.0 0.0 3.5888681632377617 4.586287393802877E-4 82-83 2.6207356536016443E-4 0.0 0.0 3.8571987349709 4.586287393802877E-4 84-85 2.6207356536016443E-4 0.0 0.0 4.132244941816393 4.586287393802877E-4 86-87 2.6207356536016443E-4 0.0 0.0 4.410501549837547 4.586287393802877E-4 88-89 2.6207356536016443E-4 0.0 0.0 4.697013475167546 4.586287393802877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6765 0.0 35.692383 1 GTATCAA 10185 0.0 33.312054 1 TGACGCG 40 4.4750172E-4 29.710506 94-95 TATCAAC 12435 0.0 27.175373 2 ATCAACG 12435 0.0 27.175373 3 TCAACGC 12565 0.0 26.856384 4 CAACGCA 12670 0.0 26.63382 5 AACGCAG 12975 0.0 26.07673 6 GTACATG 8855 0.0 24.735287 1 TACATGG 8735 0.0 24.6483 2 ACGCAGA 14295 0.0 23.629364 7 CGCAGAG 14350 0.0 23.605011 8 ACATGGG 8875 0.0 23.509743 3 GAGTACT 7965 0.0 23.080706 12-13 GCAGAGT 15135 0.0 21.971918 9 AGTACTT 8355 0.0 21.463196 12-13 GTACTTT 8675 0.0 21.138304 14-15 GTACCGT 135 3.785713E-4 21.120197 6 TACCTGG 1110 0.0 20.552752 2 CATGGGG 3420 0.0 20.289831 4 >>END_MODULE