FastQCFastQC Report
Thu 26 May 2016
SRR937927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526289
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118730.7778998603803081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100390.6577391306626726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68640.4497182381580421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47450.31088476690849504No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40610.26607018723190695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24810.16255112891464196No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.14997159777735408No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20060.13142989302812247No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16560.10849845605910807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67550.036.6886021
GTATCAA100350.030.6694321
GTACATG87150.025.3261641
TACATGG85250.025.0536352
TATCAAC123000.024.9057462
ATCAACG124550.024.4788133
TCAACGC124650.024.458374
CAACGCA127050.023.9947745
AACGCAG130150.023.573486
TACCTGG11450.023.2649082
ACATGGG89450.023.0241493
GAGTACT83050.022.36003312-13
GTACGCT1302.9155583E-421.95253
ACGCAGA145150.021.0719227
CGCAGAG146100.020.9023978
CATGGGG31750.020.6726994
GTACTTT90950.020.44393214-15
AGTACTT86900.020.27633712-13
GTACTAG3102.5465852E-1019.9480511
GCAGAGT153800.019.8250379
Produced by FastQC (version 0.11.5)