##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937927_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1526289 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.374026806194635 33.0 31.0 34.0 31.0 34.0 2 32.603011618376335 34.0 31.0 34.0 31.0 34.0 3 32.66232869397604 34.0 31.0 34.0 31.0 34.0 4 36.16219863996923 37.0 35.0 37.0 35.0 37.0 5 36.0849072488893 37.0 35.0 37.0 35.0 37.0 6 36.14938127707138 37.0 36.0 37.0 35.0 37.0 7 36.11332323039739 37.0 36.0 37.0 35.0 37.0 8 36.1183596291397 37.0 36.0 37.0 35.0 37.0 9 37.77592054977792 39.0 38.0 39.0 35.0 39.0 10-11 37.74549446402352 39.0 37.5 39.0 35.0 39.0 12-13 37.653119756481246 39.0 37.0 39.0 35.0 39.0 14-15 38.82201928992478 40.0 38.0 41.0 35.0 41.0 16-17 38.71826927927804 40.0 38.0 41.0 34.5 41.0 18-19 39.00216538283379 40.0 38.5 41.0 35.5 41.0 20-21 38.97513020142319 40.0 38.0 41.0 35.0 41.0 22-23 38.840307766091485 40.0 38.0 41.0 35.0 41.0 24-25 38.705572470220254 40.0 38.0 41.0 34.5 41.0 26-27 38.51356492774305 40.0 38.0 41.0 34.0 41.0 28-29 38.36676212696285 40.0 38.0 41.0 34.0 41.0 30-31 38.0850707172757 40.0 37.5 41.0 34.0 41.0 32-33 37.806857023800866 40.0 37.0 41.0 33.0 41.0 34-35 37.667669425646125 40.0 37.0 41.0 33.0 41.0 36-37 37.5832352195423 40.0 37.0 41.0 33.0 41.0 38-39 37.37315148048633 40.0 36.0 41.0 33.0 41.0 40-41 37.120171867844164 39.5 36.0 41.0 31.5 41.0 42-43 36.8245967834401 39.0 35.0 41.0 31.0 41.0 44-45 36.49999639648848 39.0 35.0 41.0 30.5 41.0 46-47 36.05811579589449 39.0 35.0 40.0 29.5 41.0 48-49 35.58567676239559 39.0 35.0 40.0 28.0 41.0 50-51 35.169815808146424 38.0 35.0 40.0 27.0 41.0 52-53 34.59547405504462 38.0 34.0 40.0 24.5 41.0 54-55 34.18220468076491 38.0 33.0 40.0 24.0 41.0 56-57 33.77463802726744 37.0 33.0 40.0 23.0 41.0 58-59 33.23742325339435 36.5 32.5 39.5 21.5 41.0 60-61 32.72282346266009 36.0 31.5 39.0 19.0 40.0 62-63 32.12388053638597 35.5 31.0 39.0 16.0 40.0 64-65 31.510026279426768 35.0 30.5 38.5 10.0 40.0 66-67 30.89580544706802 35.0 29.5 38.0 7.5 40.0 68-69 30.244504808722333 34.5 29.0 37.5 4.0 39.5 70-71 29.596855510326026 34.0 28.0 37.0 2.0 39.0 72-73 28.87909105025326 34.0 26.5 36.0 2.0 39.0 74-75 28.192151355346205 33.0 26.0 35.5 2.0 38.5 76-77 26.931624679205576 31.5 25.0 34.5 2.0 36.0 78-79 27.57232968330375 33.0 26.0 35.0 2.0 37.0 80-81 27.260655747371565 33.0 26.0 35.0 2.0 36.5 82-83 26.661132983334085 33.0 24.5 35.0 2.0 36.0 84-85 25.960290285784673 32.0 22.5 35.0 2.0 36.0 86-87 25.180522168475303 31.5 19.0 34.5 2.0 35.0 88-89 24.302876126343044 31.0 14.0 34.0 2.0 35.0 90-91 23.58236579048922 31.0 4.5 34.0 2.0 35.0 92-93 22.572228129797175 30.0 2.0 34.0 2.0 35.0 94-95 21.599791389441975 29.0 2.0 34.0 2.0 35.0 96-97 20.488742629999955 29.0 2.0 34.0 2.0 35.0 98-99 19.04496330642493 26.5 2.0 33.5 2.0 35.0 100-101 15.447002173245043 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2333749104388474 1101 2 0.25725972228744354 1101 3 0.29549972744913333 1101 4 0.16421621652715146 1101 5 0.07894216602170445 1101 6 0.10822981471014259 1101 7 0.16430225117381525 1101 8 0.17113118388252246 1101 9 0.2077970141040879 1101 10-11 0.26206287672606265 1101 12-13 0.3017343355144746 1101 14-15 0.45047979284816364 1101 16-17 0.48912069570886985 1101 18-19 0.43868958927366464 1101 20-21 0.32355803808959394 1101 22-23 0.4362469369906421 1101 24-25 0.4827424270485139 1101 26-27 0.4792808701481093 1101 28-29 0.5479177686650587 1101 30-31 0.6308410296526077 1101 32-33 0.7586606496011115 1101 34-35 0.6741602442307055 1101 36-37 0.7447497376640868 1101 38-39 0.8379934645153497 1101 40-41 0.8688536100900279 1101 42-43 0.9629416707223584 1101 44-45 0.984864827790723 1101 46-47 0.85130106245051 1101 48-49 1.1163921364906315 1101 50-51 1.165320739965864 1101 52-53 1.210338798164031 1101 54-55 1.342576323142552 1101 56-57 1.3279357452398628 1101 58-59 1.4615378640508183 1101 60-61 1.4422241498719828 1101 62-63 1.6599227805674417 1101 64-65 1.6700321245232317 1101 66-67 2.090737961087612 1101 68-69 2.1732860722466008 1101 70-71 2.3484804158363737 1101 72-73 2.5089974417509886 1101 74-75 2.687257195822365 1101 76-77 2.53464766989649 1101 78-79 2.7098500367494616 1101 80-81 2.7876818370073515 1101 82-83 2.996328309869508 1101 84-85 3.06684165299513 1101 86-87 3.3209446894738903 1101 88-89 3.7441613142292134 1101 90-91 4.033763851163915 1101 92-93 4.375810736612447 1101 94-95 4.346669081864967 1101 96-97 4.662760958022687 1101 98-99 4.966040706279497 1101 100-101 4.580341429879548 1102 1 0.16881975532613325 1102 2 0.10843631758106653 1102 3 0.16843591570438576 1102 4 0.032101171653998506 1102 5 -0.04517061752716245 1102 6 0.017123885800522487 1102 7 3.788535340731869E-4 1102 8 0.13740353212646284 1102 9 0.17859483757946037 1102 10-11 0.23532978365842894 1102 12-13 0.30759823205888637 1102 14-15 0.32691255378378514 1102 16-17 0.3848708193750383 1102 18-19 0.3398061181408707 1102 20-21 0.2392318420610522 1102 22-23 0.35201119393362035 1102 24-25 0.3954114963722333 1102 26-27 0.41623514899766434 1102 28-29 0.27763722312057126 1102 30-31 0.35879241118041705 1102 32-33 0.29042942919266324 1102 34-35 0.2940938178297472 1102 36-37 0.27036630186449884 1102 38-39 0.42552084773915055 1102 40-41 0.33402780272659527 1102 42-43 0.3996966703690248 1102 44-45 0.5190320244552638 1102 46-47 0.5507880245026655 1102 48-49 0.7331160429283301 1102 50-51 0.6238310925911179 1102 52-53 0.9014235271584496 1102 54-55 1.1550418732803465 1102 56-57 0.9112160787851948 1102 58-59 1.0804948282229603 1102 60-61 0.9607542890847611 1102 62-63 1.25270208945021 1102 64-65 1.1879587728166427 1102 66-67 1.3304439889301705 1102 68-69 1.6569324576610533 1102 70-71 1.8739075941276617 1102 72-73 2.086705024253991 1102 74-75 2.1928016803110886 1102 76-77 2.363458354653755 1102 78-79 2.285786719669396 1102 80-81 2.4526482704749526 1102 82-83 2.3107075056860573 1102 84-85 2.6166331871623285 1102 86-87 2.6817361529747004 1102 88-89 2.7360205467922825 1102 90-91 2.8581494069550253 1102 92-93 2.8778049419689573 1102 94-95 3.114073505580606 1102 96-97 3.2032754092982145 1102 98-99 3.2384822298467384 1102 100-101 2.9684143575878377 1103 1 0.1692185374240509 1103 2 0.1929323162877168 1103 3 0.20154560772604668 1103 4 0.0944836252416863 1103 5 0.011324411168985193 1103 6 0.0772429815351714 1103 7 0.209305644685422 1103 8 0.11093571761402643 1103 9 0.2452776710879334 1103 10-11 0.3439143766581907 1103 12-13 0.35075157455343486 1103 14-15 0.48195121948044317 1103 16-17 0.38075501089821984 1103 18-19 0.2855879196659501 1103 20-21 0.3904712999329547 1103 22-23 0.36523567053211536 1103 24-25 0.4161495127007484 1103 26-27 0.4698713411675257 1103 28-29 0.5129469528648656 1103 30-31 0.591487154262353 1103 32-33 0.5825265380224494 1103 34-35 0.7291772117887376 1103 36-37 0.6570071015842771 1103 38-39 0.6347696284898348 1103 40-41 0.7178097659200802 1103 42-43 0.6949139796676462 1103 44-45 0.8217251508530197 1103 46-47 0.9212997050458753 1103 48-49 1.0400408401691976 1103 50-51 1.2037909449339637 1103 52-53 1.2708695433791846 1103 54-55 1.3829862593445341 1103 56-57 1.5217595541172884 1103 58-59 1.8591990558520948 1103 60-61 1.7832639218280022 1103 62-63 1.762866991236642 1103 64-65 1.8632396716930444 1103 66-67 1.8670322464140696 1103 68-69 1.9490713308321865 1103 70-71 2.0401310198814393 1103 72-73 2.277204310218476 1103 74-75 2.3824818462906663 1103 76-77 2.504974804415294 1103 78-79 2.5876849763449563 1103 80-81 2.7445136545721702 1103 82-83 2.9235049082134736 1103 84-85 3.1204862844597727 1103 86-87 3.162864059638135 1103 88-89 3.236865264276723 1103 90-91 3.536357851435394 1103 92-93 3.5071450653758482 1103 94-95 3.7832891443055843 1103 96-97 3.9182027932337604 1103 98-99 3.986984102505911 1103 100-101 3.9826815954829176 1104 1 0.14883060748531562 1104 2 0.10028925758980023 1104 3 0.11622644256348025 1104 4 0.11095666589044129 1104 5 -0.027923625031029076 1104 6 0.04276145015250421 1104 7 0.15897389595013323 1104 8 0.12785363204573486 1104 9 0.1732640452394918 1104 10-11 0.27040343276313195 1104 12-13 0.2872147008721626 1104 14-15 0.38322864277149193 1104 16-17 0.5035643252841737 1104 18-19 0.3912797619518358 1104 20-21 0.4432193488436482 1104 22-23 0.3019059809269038 1104 24-25 0.2924377400693814 1104 26-27 0.3584368138776952 1104 28-29 0.4013306628580011 1104 30-31 0.45899357315551015 1104 32-33 0.5111001455675108 1104 34-35 0.43352553525011217 1104 36-37 0.5269285495848877 1104 38-39 0.6303196195923562 1104 40-41 0.6791489631135619 1104 42-43 0.820617515778693 1104 44-45 0.8410619075960355 1104 46-47 0.8144359381088933 1104 48-49 0.8972668614722892 1104 50-51 1.2105517553668932 1104 52-53 1.2074292708622139 1104 54-55 1.2745717895069717 1104 56-57 1.4863196374993422 1104 58-59 1.4642007082404334 1104 60-61 1.617587840784708 1104 62-63 1.763981717829921 1104 64-65 1.763239671693043 1104 66-67 1.8893725367259862 1104 68-69 2.0641067936280173 1104 70-71 2.1767543007620347 1104 72-73 2.2504508719796696 1104 74-75 2.2226755570464434 1104 76-77 2.211792812906026 1104 78-79 2.4306127542639615 1104 80-81 2.419181270302907 1104 82-83 2.7982831068559726 1104 84-85 2.951467294704578 1104 86-87 3.0480891657723106 1104 88-89 3.247828225356624 1104 90-91 3.6058433231287097 1104 92-93 3.7941572930495404 1104 94-95 3.8041702486094167 1104 96-97 4.130450804012419 1104 98-99 4.455712017700261 1104 100-101 3.962458904377849 1105 1 0.1746879323998769 1105 2 0.12430787158118761 1105 3 0.13712566826777817 1105 4 0.04816920137094627 1105 5 -0.08055159622182373 1105 6 -0.008236831335842965 1105 7 0.02738658976542041 1105 8 0.02695961980375472 1105 9 0.10069989068929885 1105 10-11 0.1421020560228925 1105 12-13 0.23449621818279098 1105 14-15 0.29940365097787236 1105 16-17 0.3028414401155075 1105 18-19 0.3084429143587215 1105 20-21 0.23288245297773358 1105 22-23 0.27363043013426847 1105 24-25 0.1787762965195938 1105 26-27 0.21950769765610545 1105 28-29 0.3333611133397696 1105 30-31 0.42908543629246765 1105 32-33 0.5280031075864713 1105 34-35 0.5777786585002218 1105 36-37 0.632115703227548 1105 38-39 0.6731040429255017 1105 40-41 0.7589525893662454 1105 42-43 0.831619369454188 1105 44-45 0.8035626316880311 1105 46-47 0.8084935565220732 1105 48-49 1.0909735366349764 1105 50-51 1.0515211214484879 1105 52-53 1.2271856380444248 1105 54-55 1.16977371462621 1105 56-57 1.2180597270936602 1105 58-59 1.5380576083543573 1105 60-61 1.6099184998049623 1105 62-63 1.59800969674437 1105 64-65 1.6568872126766507 1105 66-67 1.81246262342394 1105 68-69 1.7701331698855292 1105 70-71 2.0081043461384667 1105 72-73 2.081593585683354 1105 74-75 2.152104345013001 1105 76-77 2.112841298105586 1105 78-79 2.0924108677629683 1105 80-81 2.2322170983745053 1105 82-83 2.3774263612150044 1105 84-85 2.6629185681410235 1105 86-87 2.890088673389755 1105 88-89 2.7973961838033965 1105 90-91 2.821612598457449 1105 92-93 3.1321327801220846 1105 94-95 3.4056104675748387 1105 96-97 3.7009484000270163 1105 98-99 3.785003228188973 1105 100-101 3.552763071285879 1106 1 0.10496269956941973 1106 2 0.07372057604536053 1106 3 0.04543767015583455 1106 4 0.006924171143836588 1106 5 -0.07682540120703152 1106 6 0.013694617568539513 1106 7 0.0765419553046982 1106 8 0.09573219936550714 1106 9 0.1328944879334628 1106 10-11 0.08904212827748381 1106 12-13 0.09735522186813483 1106 14-15 0.024685271441533985 1106 16-17 0.004320344677708476 1106 18-19 -0.06042656442784278 1106 20-21 -0.015882819429357653 1106 22-23 -0.11769652191990332 1106 24-25 -0.06414279830095637 1106 26-27 -0.1207116077557302 1106 28-29 0.14270746339332163 1106 30-31 0.10338036394709604 1106 32-33 0.19215152905952237 1106 34-35 0.19775079146319996 1106 36-37 0.19495972951515483 1106 38-39 0.07107188236779649 1106 40-41 0.13671796162265792 1106 42-43 0.06740854215719594 1106 44-45 0.15532179513228783 1106 46-47 0.21952145986792715 1106 48-49 0.21697384526014218 1106 50-51 0.5158215862016817 1106 52-53 0.4520438066263921 1106 54-55 0.3532388944457594 1106 56-57 0.505494825036763 1106 58-59 0.5947842753841073 1106 60-61 0.3620110238141905 1106 62-63 0.4944654998052016 1106 64-65 0.6359971467761056 1106 66-67 0.9682368641152586 1106 68-69 0.8865901529837643 1106 70-71 0.7940053954640298 1106 72-73 1.175650735802261 1106 74-75 1.4196385660975466 1106 76-77 1.4029671082466457 1106 78-79 1.55188225639467 1106 80-81 1.5954183129053021 1106 82-83 2.12325992147861 1106 84-85 2.221652660329177 1106 86-87 2.3935818110184286 1106 88-89 2.4141794926282607 1106 90-91 2.66298970210104 1106 92-93 2.730907583600473 1106 94-95 3.138847355543902 1106 96-97 3.343865452475139 1106 98-99 3.0044259629710304 1106 100-101 2.6241237905640578 1107 1 0.2675250773772788 1107 2 0.2946849882242404 1107 3 0.3393395700870201 1107 4 0.24523907026838287 1107 5 0.12250558968296588 1107 6 0.17892163860951626 1107 7 0.25227077875101145 1107 8 0.22034152630041604 1107 9 0.30464252488390997 1107 10-11 0.32481360789049063 1107 12-13 0.3211900385970097 1107 14-15 0.524623748124192 1107 16-17 0.5097594616586036 1107 18-19 0.4767722561473846 1107 20-21 0.4594265061383638 1107 22-23 0.4785667340763382 1107 24-25 0.4839397249753645 1107 26-27 0.5497827207058705 1107 28-29 0.6000328397109485 1107 30-31 0.5797030013377764 1107 32-33 0.6240039733581284 1107 34-35 0.699146915038483 1107 36-37 0.834314144838558 1107 38-39 0.830375119405744 1107 40-41 0.7879896713784333 1107 42-43 0.8521758245127629 1107 44-45 0.7791073932072905 1107 46-47 0.7963221562207323 1107 48-49 1.073587829738031 1107 50-51 1.136466532858691 1107 52-53 1.1591761820188893 1107 54-55 1.2429412583083277 1107 56-57 1.1636243338984045 1107 58-59 1.1812500570264675 1107 60-61 1.2392487635842286 1107 62-63 1.4251786234123784 1107 64-65 1.5794948157259654 1107 66-67 1.87201394007149 1107 68-69 2.2201882644457847 1107 70-71 2.2139963720441536 1107 72-73 2.345648320442397 1107 74-75 2.1526173428317072 1107 76-77 2.34706064191813 1107 78-79 2.215641376656805 1107 80-81 2.429836507401017 1107 82-83 2.901341897881 1107 84-85 2.8411719581430432 1107 86-87 3.15354608192154 1107 88-89 3.5610039779971068 1107 90-91 3.5757924765747866 1107 92-93 3.706851966521601 1107 94-95 3.8564765203306877 1107 96-97 4.123703577791304 1107 98-99 4.27649027534542 1107 100-101 3.763728856439579 1108 1 0.12191902594704374 1108 2 0.13845923042564579 1108 3 0.14887889736623094 1108 4 0.07352906443342988 1108 5 0.0745667115123112 1108 6 0.03689874676181404 1108 7 0.0711383369058538 1108 8 0.06891013695732084 1108 9 0.20370028128289164 1108 10-11 0.22112067048787765 1108 12-13 0.27079437703184084 1108 14-15 0.37184272603693813 1108 16-17 0.21499976646027363 1108 18-19 0.2817195637550185 1108 20-21 0.2048686117961509 1108 22-23 0.14042485345347444 1108 24-25 0.18821875289373224 1108 26-27 0.28713399915606175 1108 28-29 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2.1049652549126066 2316 76-77 2.2332550373717837 2316 78-79 2.2880395099937836 2316 80-81 2.0755955000592365 2316 82-83 2.4114659015450535 2316 84-85 2.82298156398366 2316 86-87 2.8502817002764544 2316 88-89 3.160414265607219 2316 90-91 3.3670871550357013 2316 92-93 3.6096451251287114 2316 94-95 4.044772051003477 2316 96-97 4.557265230991199 2316 98-99 5.131336261974351 2316 100-101 5.0488746211288404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 19.0 5 32.0 6 89.0 7 246.0 8 497.0 9 1002.0 10 1653.0 11 2626.0 12 4009.0 13 6195.0 14 7936.0 15 10829.0 16 14230.0 17 19579.0 18 20516.0 19 16633.0 20 16135.0 21 17188.0 22 18859.0 23 20933.0 24 23354.0 25 26484.0 26 29508.0 27 33898.0 28 40586.0 29 48397.0 30 56993.0 31 66982.0 32 80020.0 33 98093.0 34 127356.0 35 171605.0 36 206122.0 37 228928.0 38 105206.0 39 3545.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46910119905208 18.990374693128235 14.374145394482959 22.166378713336726 2 15.95656567112735 20.572790048189013 39.95664692537845 23.513997355305182 3 19.712300449796725 25.410271614321424 29.675248731363574 25.202179204518277 4 12.860061273642637 16.851758668857634 36.411822825349155 33.876357232150575 5 13.10497691312131 38.20968619031407 33.925477825248166 14.759859071316447 6 31.60390987552161 35.98479711247345 17.37082557759376 15.040467434411175 7 28.15253205651092 33.58721709977599 20.33808800299288 17.922162840720205 8 26.532462810025653 34.826523224692636 19.509659074157184 19.131354891124527 9 26.778414834936243 16.73542821837804 20.258352120732052 36.22780482595367 10-11 25.101275053413868 26.96730435716958 26.521025834556887 21.410394754859663 12-13 26.211025565931482 24.51272334400628 27.459445753720296 21.816805336341936 14-15 22.88917760660006 25.578478256739057 26.579369962045195 24.952974174615687 16-17 21.472309634676 28.838738928210844 27.371421794954955 22.317529642158203 18-19 21.336064139884385 26.99872042581713 30.374227947656046 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21.491562715092698 27.016936814631332 29.92947007056269 21.56203039971328 46-47 21.725636494792273 27.14384366263532 29.39711941840634 21.733400424166067 48-49 21.750959353045197 27.177389079001422 29.287572668085794 21.784078899867588 50-51 21.805536173031452 27.49246702295568 29.131606137500825 21.570390666512044 52-53 21.92861247116372 27.435891892033553 29.015966176785657 21.619529460017073 54-55 21.596761819026412 27.48732382923549 29.07191888299005 21.843995468748055 56-57 21.80514306268341 27.610793237715793 28.752582243598688 21.83148145600211 58-59 21.74109883514852 27.336566010762052 28.87044982961942 22.051885324470007 60-61 21.574747639536156 27.47385979981511 29.10317115565925 21.848221404989488 62-63 21.670633805262305 27.375647731196384 29.11814210808045 21.83557635546086 64-65 21.48708403192318 27.418922628676484 29.05390132537154 22.040092014028797 66-67 21.49854975040769 27.613807083717433 28.827797356857054 22.05984580901782 68-69 21.71822697279841 27.46527320508612 28.852477705466463 21.96402211664901 70-71 21.570914813642762 27.457447442784428 28.606410712519057 22.365227031053752 72-73 21.561435836957322 27.51064265415264 28.520019983128996 22.407901525761037 74-75 21.600004979397742 27.778454801154957 28.26122706774405 22.36031315170325 76-77 21.576254562536974 27.407784502148676 28.735875053806982 22.280085881507368 78-79 21.58231501373593 27.428127962659758 28.71661264675301 22.272944376851303 80-81 21.52154919800444 27.679272535005715 28.557646287246857 22.24153197974299 82-83 21.563069818699784 27.635817914854222 28.475521424036515 22.325590842409483 84-85 21.612765631234755 27.46689048716996 28.370624738324608 22.549719143270675 86-87 21.562307329931095 27.436512652915745 28.46701291859978 22.534167098553382 88-89 21.639119459027746 27.500165433938133 28.19328449592443 22.667430611109694 90-91 21.582055276274904 27.386801245542564 28.511032814248065 22.520110663934467 92-93 21.45291619083935 27.3845909916143 28.37444284797964 22.788049969566707 94-95 21.318767284570615 27.355533585054992 28.319178084884317 23.006521045490075 96-97 21.405265585525143 27.019966087658425 28.29986870105773 23.274899625758703 98-99 21.31398444200279 27.086678866191132 28.144145702419397 23.455190989386676 100-101 21.779754686039144 26.517291286250504 28.27652561212195 23.4264284155884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4499.0 1 2877.0 2 2770.5 3 3586.5 4 2183.5 5 1691.0 6 2848.0 7 5199.0 8 7235.0 9 7401.0 10 5359.0 11 3337.5 12 2873.5 13 2887.5 14 2915.0 15 2897.0 16 2851.5 17 2954.0 18 3121.0 19 3351.0 20 3628.5 21 4060.5 22 4722.5 23 5692.0 24 6890.5 25 8130.5 26 9852.0 27 12042.5 28 14037.0 29 15950.0 30 18192.0 31 20845.5 32 24093.0 33 26201.5 34 27553.0 35 29745.5 36 32867.0 37 35852.5 38 37675.5 39 39077.0 40 40543.0 41 42459.5 42 43448.0 43 44426.0 44 46626.5 45 49295.5 46 51295.5 47 53188.0 48 57966.0 49 58847.0 50 55488.5 51 53402.5 52 51630.0 53 50727.5 54 50053.5 55 47398.0 56 43376.0 57 39653.5 58 36224.5 59 32400.5 60 27531.0 61 23204.5 62 19285.5 63 14897.0 64 10795.5 65 8070.0 66 6298.5 67 4914.5 68 3795.5 69 2869.5 70 2176.0 71 1554.0 72 1209.0 73 1041.5 74 808.0 75 585.0 76 408.5 77 276.5 78 174.0 79 101.0 80 62.5 81 34.0 82 19.0 83 12.5 84 7.0 85 4.5 86 4.5 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014086454138108839 3 0.002882809218961809 4 0.0013758862181408632 5 0.11989865615227523 6 0.0 7 0.0 8 2.6207356536016443E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.3103678268008221E-4 24-25 3.9311034804024664E-4 26-27 0.005765618437923618 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01117088572347701 36-37 8.84498283090555E-4 38-39 0.006879431090704316 40-41 0.009893277092346206 42-43 0.00592941441627372 44-45 0.0037673075020523638 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0011793310441207399 70-71 0.0 72-73 9.827758701006166E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6379597835010278E-4 82-83 0.0023586620882414797 84-85 0.004324213828442713 86-87 0.0026207356536016445 88-89 0.0 90-91 0.003046605197311911 92-93 0.0 94-95 0.0 96-97 6.551839134004111E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1526289.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.23651698727007 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7133854126298 47.414201775334114 2 12.87432543782478 15.51009045870351 3 3.876911244580264 7.005948901268923 4 1.5994242108452155 3.8537497458571544 5 0.793838766162004 2.3909041161535534 6 0.4794013653087089 1.7326481091083108 7 0.3168950732837092 1.336205882552544 8 0.22904106910026073 1.103730899971228 9 0.16629443512647576 0.9015297809745995 >10 0.868425548633751 9.433492947346133 >50 0.05209213370658841 2.1477695925770988 >100 0.02681689559979272 3.12340109875299 >500 0.001737052247521855 0.723282367503014 >1k 0.0010856576547011595 1.441202243673727 >5k 1.0856576547011594E-4 0.44887976225503506 >10k+ 2.1713153094023188E-4 1.4329623179679964 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11873 0.7778998603803081 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10039 0.6577391306626726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6864 0.4497182381580421 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4745 0.31088476690849504 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4061 0.26607018723190695 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2481 0.16255112891464196 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2289 0.14997159777735408 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2006 0.13142989302812247 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1656 0.10849845605910807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.2759195670020554E-5 0.0 16-17 0.0 0.0 0.0 6.551839134004111E-5 0.0 18-19 0.0 0.0 0.0 6.551839134004111E-5 0.0 20-21 0.0 0.0 0.0 6.551839134004111E-5 0.0 22-23 0.0 0.0 0.0 2.2931436969014386E-4 0.0 24-25 0.0 0.0 0.0 7.534615004104728E-4 0.0 26-27 0.0 0.0 0.0 0.0020965885228813154 0.0 28-29 0.0 0.0 0.0 0.005405267285553392 0.0 30-31 0.0 0.0 0.0 0.016281320248000213 0.0 32-33 0.0 0.0 0.0 0.03727996467248339 0.0 34-35 0.0 0.0 0.0 0.06545287294870107 0.0 36-37 0.0 3.2759195670020554E-5 0.0 0.11639342221558302 0.0 38-39 0.0 6.551839134004111E-5 0.0 0.18931539177704879 0.0 40-41 0.0 6.551839134004111E-5 0.0 0.26797022058076814 0.0 42-43 0.0 6.551839134004111E-5 0.0 0.3437422401655257 0.0 44-45 0.0 6.551839134004111E-5 0.0 0.43871114841291525 0.0 46-47 0.0 6.551839134004111E-5 0.0 0.5513045039307759 0.0 48-49 0.0 6.551839134004111E-5 0.0 0.6672392974069786 0.0 50-51 0.0 6.551839134004111E-5 0.0 0.7914949265833666 0.0 52-53 0.0 6.551839134004111E-5 0.0 0.9047762252102977 0.0 54-55 0.0 6.551839134004111E-5 0.0 1.031488794061937 0.0 56-57 0.0 6.551839134004111E-5 0.0 1.173041278552096 3.2759195670020554E-5 58-59 0.0 6.551839134004111E-5 0.0 1.3266163878531523 6.551839134004111E-5 60-61 6.551839134004111E-5 6.551839134004111E-5 0.0 1.4998142553605511 6.551839134004111E-5 62-63 9.827758701006166E-5 6.551839134004111E-5 0.0 1.662332625079523 6.551839134004111E-5 64-65 1.3103678268008221E-4 6.551839134004111E-5 0.0 1.8228526838626236 6.551839134004111E-5 66-67 1.3103678268008221E-4 6.551839134004111E-5 0.0 2.0061403836363887 6.551839134004111E-5 68-69 1.3103678268008221E-4 6.551839134004111E-5 0.0 2.2096077479428864 6.551839134004111E-5 70-71 1.3103678268008221E-4 6.551839134004111E-5 0.0 2.4264081048870825 6.551839134004111E-5 72-73 1.3103678268008221E-4 6.551839134004111E-5 0.0 2.639211839959536 9.827758701006166E-5 74-75 1.3103678268008221E-4 6.551839134004111E-5 0.0 2.8507707255965284 1.6379597835010275E-4 76-77 1.3103678268008221E-4 6.551839134004111E-5 0.0 3.080740279200073 1.9655517402012332E-4 78-79 1.3103678268008221E-4 6.551839134004111E-5 0.0 3.33183951401078 1.9655517402012332E-4 80-81 1.3103678268008221E-4 6.551839134004111E-5 0.0 3.6007269920703093 2.6207356536016443E-4 82-83 1.3103678268008221E-4 6.551839134004111E-5 0.0 3.87325074084921 2.6207356536016443E-4 84-85 1.9655517402012332E-4 6.551839134004111E-5 0.0 4.154717750046027 2.6207356536016443E-4 86-87 1.9655517402012332E-4 6.551839134004111E-5 0.0 4.438838254092115 2.6207356536016443E-4 88-89 1.9655517402012332E-4 6.551839134004111E-5 0.0 4.731967536947459 2.6207356536016443E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6755 0.0 36.688602 1 GTATCAA 10035 0.0 30.669432 1 GTACATG 8715 0.0 25.326164 1 TACATGG 8525 0.0 25.053635 2 TATCAAC 12300 0.0 24.905746 2 ATCAACG 12455 0.0 24.478813 3 TCAACGC 12465 0.0 24.45837 4 CAACGCA 12705 0.0 23.994774 5 AACGCAG 13015 0.0 23.57348 6 TACCTGG 1145 0.0 23.264908 2 ACATGGG 8945 0.0 23.024149 3 GAGTACT 8305 0.0 22.360033 12-13 GTACGCT 130 2.9155583E-4 21.9525 3 ACGCAGA 14515 0.0 21.071922 7 CGCAGAG 14610 0.0 20.902397 8 CATGGGG 3175 0.0 20.672699 4 GTACTTT 9095 0.0 20.443932 14-15 AGTACTT 8690 0.0 20.276337 12-13 GTACTAG 310 2.5465852E-10 19.948051 1 GCAGAGT 15380 0.0 19.825037 9 >>END_MODULE