FastQCFastQC Report
Thu 26 May 2016
SRR937926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531890
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129350.8443817767594279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100300.6547467507458107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66650.4350834589950976No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46650.30452578187728885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.28546436101808875No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.15157746313377593No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.14485374276220878No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.1334299460144005No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17830.11639216915052648No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15640.10209610350612641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70400.033.5352361
GTATCAA106050.031.9312931
ATCAACG130500.025.787723
TATCAAC130950.025.6982652
TCAACGC131350.025.6208424
CAACGCA132400.025.3817545
GTACATG93800.024.8133721
AACGCAG136400.024.7379256
TACATGG91700.024.6725272
GAGTACT80450.023.2950112-13
ACATGGG94200.023.2616353
ACGCAGA148500.022.6188417
CGCAGAG149850.022.3516588
TACCTGG12450.021.7611352
GTACTTT87850.021.19896114-15
GCAGAGT157000.021.090279
AGTACTT83650.021.04089512-13
CATGGGG34150.021.0172794
GTACCTG13300.020.799131
GTGTAGC11900.020.4405251
Produced by FastQC (version 0.11.5)