##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937926_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.076258086416125 33.0 31.0 34.0 31.0 34.0 2 32.254725208729084 34.0 31.0 34.0 31.0 34.0 3 32.33440978138117 34.0 31.0 34.0 30.0 34.0 4 35.8494291365568 37.0 35.0 37.0 35.0 37.0 5 35.86643753794333 37.0 35.0 37.0 35.0 37.0 6 35.90437890449053 37.0 37.0 37.0 35.0 37.0 7 35.855573180841965 37.0 36.0 37.0 35.0 37.0 8 35.83516244638975 37.0 36.0 37.0 35.0 37.0 9 37.5088648662763 39.0 38.0 39.0 35.0 39.0 10-11 37.431841711872266 39.0 37.5 39.0 35.0 39.0 12-13 37.33587431212424 39.0 37.0 39.0 35.0 39.0 14-15 38.551743271383714 40.0 38.0 41.0 34.5 41.0 16-17 38.40922030955225 40.0 38.0 41.0 34.0 41.0 18-19 38.56698065788014 40.0 38.0 41.0 34.0 41.0 20-21 38.55634836704985 40.0 38.0 41.0 34.0 41.0 22-23 38.454719986422006 40.0 38.0 41.0 34.0 41.0 24-25 38.34931359301255 40.0 38.0 41.0 34.0 41.0 26-27 38.170019714209246 40.0 38.0 41.0 34.0 41.0 28-29 37.96303128814732 40.0 38.0 41.0 33.5 41.0 30-31 37.73261722447434 40.0 37.5 41.0 33.0 41.0 32-33 37.46258053776707 40.0 36.5 41.0 33.0 41.0 34-35 37.22835321073967 40.0 36.0 41.0 32.0 41.0 36-37 36.97228717466659 39.5 36.0 41.0 31.0 41.0 38-39 36.697412673233714 39.0 35.0 41.0 31.0 41.0 40-41 36.47720430318104 39.0 35.0 41.0 30.0 41.0 42-43 36.297335317810024 39.0 35.0 41.0 30.0 41.0 44-45 36.019826162452915 39.0 35.0 41.0 29.5 41.0 46-47 35.606840895886776 39.0 35.0 40.0 27.5 41.0 48-49 35.20350775838996 39.0 35.0 40.0 26.0 41.0 50-51 34.4923401810835 37.5 34.0 39.5 24.5 40.5 52-53 34.6155011130042 38.0 34.5 40.0 26.0 40.5 54-55 34.735678475608566 38.0 34.5 40.0 25.5 41.0 56-57 34.46361749211758 38.0 34.0 40.0 24.0 41.0 58-59 34.16610298389571 38.0 34.0 40.0 23.0 41.0 60-61 33.77260116588006 37.0 33.5 40.0 22.0 41.0 62-63 33.26085750282331 36.5 33.0 40.0 20.5 41.0 64-65 32.76865310172401 36.0 32.5 39.0 18.0 41.0 66-67 32.26288767470249 35.5 32.0 39.0 14.0 40.0 68-69 31.691110001370856 35.0 31.0 38.5 8.0 40.0 70-71 31.17396190326982 35.0 31.0 38.0 6.0 40.0 72-73 30.563363557435586 35.0 30.0 37.0 2.0 39.0 74-75 29.913643930047193 34.5 29.0 36.5 2.0 39.0 76-77 29.26981604423294 34.0 29.0 36.0 2.0 38.5 78-79 28.584397704796036 34.0 27.5 35.0 2.0 37.5 80-81 27.961079124480214 33.5 26.5 35.0 2.0 37.0 82-83 27.262164711565454 33.0 25.5 35.0 2.0 36.5 84-85 26.62789527968718 33.0 24.5 35.0 2.0 36.0 86-87 25.96505297377749 32.0 23.0 35.0 2.0 35.5 88-89 25.19998041634843 31.5 19.5 34.5 2.0 35.0 90-91 24.408707870669566 31.0 14.5 34.0 2.0 35.0 92-93 23.551583338229246 31.0 4.5 34.0 2.0 35.0 94-95 22.64482371449647 30.0 2.0 34.0 2.0 35.0 96-97 21.487568297984843 29.5 2.0 34.0 2.0 35.0 98-99 20.038703823381574 29.0 2.0 34.0 2.0 35.0 100-101 16.29851751757633 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35247531502493956 1101 2 0.3604255105462144 1101 3 0.22156868609594227 1101 4 0.16990349911274905 1101 5 0.1680345823013525 1101 6 0.2926053631363672 1101 7 0.3043216878589874 1101 8 0.15092351689207106 1101 9 0.2587789982284221 1101 10-11 0.36442715094588607 1101 12-13 0.32012533609039195 1101 14-15 0.5054591522283047 1101 16-17 0.5254571669472199 1101 18-19 0.3211736187117893 1101 20-21 0.43648370494186395 1101 22-23 0.49730472989259056 1101 24-25 0.41046833409128425 1101 26-27 0.5463380203628958 1101 28-29 0.3990740345161754 1101 30-31 0.391358751559828 1101 32-33 0.4658800154413285 1101 34-35 0.49759317230944333 1101 36-37 0.5940229674396633 1101 38-39 0.6889335537121326 1101 40-41 0.6018448957149332 1101 42-43 0.7714250365467663 1101 44-45 0.7593544583649106 1101 46-47 0.7339939249775043 1101 48-49 0.625716603463708 1101 50-51 0.7240312033597789 1101 52-53 0.6949471852689086 1101 54-55 1.018567896916423 1101 56-57 0.9574205492952501 1101 58-59 0.9941217430472093 1101 60-61 0.9774483929269167 1101 62-63 0.9457531288523953 1101 64-65 0.8606552138406727 1101 66-67 1.0390094201934161 1101 68-69 1.333836600355042 1101 70-71 1.3996008575357202 1101 72-73 1.6214156250443743 1101 74-75 1.7328724191174665 1101 76-77 1.817729405854653 1101 78-79 1.8782622083051308 1101 80-81 1.8222472975443758 1101 82-83 2.0219310262764587 1101 84-85 1.867579110526723 1101 86-87 2.1669243258186626 1101 88-89 2.41392296987728 1101 90-91 2.6064179473540605 1101 92-93 3.08739336650099 1101 94-95 2.978451191892752 1101 96-97 3.3285489470392022 1101 98-99 3.6347887697068266 1101 100-101 3.8108158463500494 1102 1 0.29137301637184976 1102 2 0.2534040902798722 1102 3 0.23045184355747494 1102 4 0.09009093143772873 1102 5 0.19534032753563224 1102 6 0.25012543090128503 1102 7 0.05185442159000786 1102 8 0.09453599426714021 1102 9 0.2265686872529784 1102 10-11 0.2273765030637449 1102 12-13 0.36347780155865195 1102 14-15 0.3750316658831707 1102 16-17 0.48053593966704966 1102 18-19 0.3913813285692598 1102 20-21 0.4484274712487917 1102 22-23 0.4110637848596852 1102 24-25 0.3765024682706155 1102 26-27 0.37647023387032874 1102 28-29 0.4644875563339994 1102 30-31 0.44299503855383904 1102 32-33 0.4891007868266328 1102 34-35 0.413754565485803 1102 36-37 0.535835439220925 1102 38-39 0.47177516520659424 1102 40-41 0.7234371714733854 1102 42-43 0.8444591147880729 1102 44-45 0.8308292642799557 1102 46-47 0.7935359524110979 1102 48-49 0.9952936121380986 1102 50-51 1.0975733186959857 1102 52-53 0.9598891295945933 1102 54-55 0.9406487313518639 1102 56-57 1.0673907023656568 1102 58-59 1.0174204281041668 1102 60-61 1.0035429801243012 1102 62-63 0.924489688744039 1102 64-65 1.0452551770814011 1102 66-67 1.2118963614624931 1102 68-69 1.3396301810676121 1102 70-71 1.463710324246609 1102 72-73 1.407321341510528 1102 74-75 1.4603843359136555 1102 76-77 1.5243454752497634 1102 78-79 1.84705378723568 1102 80-81 1.688134394640791 1102 82-83 1.9230266123670745 1102 84-85 1.8936736977241075 1102 86-87 2.148007285898892 1102 88-89 2.4521601031336324 1102 90-91 2.4814594014229527 1102 92-93 2.7640681108842102 1102 94-95 3.2228434053202726 1102 96-97 3.35217876520068 1102 98-99 3.6078041686448046 1102 100-101 3.630355796085741 1103 1 0.3464127007819897 1103 2 0.3239090316445967 1103 3 0.3329514719127076 1103 4 0.27368586643313364 1103 5 0.28153230411778196 1103 6 0.35472379469740645 1103 7 0.30584310666850456 1103 8 0.2848265781784889 1103 9 0.38805408191555557 1103 10-11 0.4904290904969528 1103 12-13 0.5993912560663048 1103 14-15 0.6478917473670407 1103 16-17 0.7094165456111909 1103 18-19 0.6445297338808231 1103 20-21 0.5068373392333214 1103 22-23 0.612052903825905 1103 24-25 0.6747615995524683 1103 26-27 0.6680557732024397 1103 28-29 0.7153677199302209 1103 30-31 0.9053601133722751 1103 32-33 0.8669613940518843 1103 34-35 0.8853210210099007 1103 36-37 0.91734904262821 1103 38-39 0.9307924155670619 1103 40-41 1.1612032902812714 1103 42-43 1.162917592700289 1103 44-45 1.1503766915916742 1103 46-47 1.0656140691208549 1103 48-49 1.0500598505974708 1103 50-51 1.1287346753746519 1103 52-53 1.1375377482242115 1103 54-55 1.1893615714068346 1103 56-57 1.1550453881717218 1103 58-59 1.3281411409853163 1103 60-61 1.3220612837804353 1103 62-63 1.354512301666638 1103 64-65 1.5175570662454447 1103 66-67 1.5474852462018518 1103 68-69 1.7589654384935614 1103 70-71 1.754532329968118 1103 72-73 1.7986499927240196 1103 74-75 1.9606052077258163 1103 76-77 2.1289278250841264 1103 78-79 2.15884169470036 1103 80-81 2.2089458976205982 1103 82-83 2.4395092091185973 1103 84-85 2.4681902875841892 1103 86-87 2.79773964123239 1103 88-89 3.092448753283559 1103 90-91 3.0895439744786444 1103 92-93 3.095809112548455 1103 94-95 3.425209422847402 1103 96-97 3.624073778875996 1103 98-99 3.945945618458257 1103 100-101 3.939725595325047 1104 1 0.3557562496839779 1104 2 0.39057233792683377 1104 3 0.33860195832835416 1104 4 0.18792549544669157 1104 5 0.2232531569833469 1104 6 0.3198480449116303 1104 7 0.25030939361172955 1104 8 0.19199259797115076 1104 9 0.1869317135166142 1104 10-11 0.3845946230338768 1104 12-13 0.382424675089446 1104 14-15 0.5438416440352185 1104 16-17 0.5450214988178388 1104 18-19 0.4828767607807052 1104 20-21 0.4546186454147474 1104 22-23 0.5627535439791345 1104 24-25 0.5903915876231025 1104 26-27 0.5986302309203921 1104 28-29 0.6261167496192144 1104 30-31 0.5813358850005486 1104 32-33 0.5399968293056787 1104 34-35 0.6010560210939104 1104 36-37 0.7979260206344989 1104 38-39 0.8253376715443395 1104 40-41 0.8159305110753294 1104 42-43 0.8795246125433565 1104 44-45 0.9267237856992381 1104 46-47 0.843995035903454 1104 48-49 0.9566646861906705 1104 50-51 1.0247458990586509 1104 52-53 0.9536776746317983 1104 54-55 1.2049789580358663 1104 56-57 1.3048765288542157 1104 58-59 1.205933200396906 1104 60-61 1.3699593633207385 1104 62-63 1.4005588093871282 1104 64-65 1.3126164610402142 1104 66-67 1.4443617688760426 1104 68-69 1.4680855218310853 1104 70-71 1.691654496744004 1104 72-73 1.8032117145850002 1104 74-75 1.785835814107191 1104 76-77 1.746686616689889 1104 78-79 1.9092079766030068 1104 80-81 2.1634992185205064 1104 82-83 2.3635342775864245 1104 84-85 2.4019201462800233 1104 86-87 2.4158050679172014 1104 88-89 2.571547162141531 1104 90-91 2.619319500798852 1104 92-93 2.7624396550825203 1104 94-95 3.020137762649476 1104 96-97 3.097376364691815 1104 98-99 3.3626568102185956 1104 100-101 3.4735285423821907 1105 1 0.24074144167744294 1105 2 0.09243610426670301 1105 3 0.022777816171490883 1105 4 0.14998125525061567 1105 5 0.12519862266962178 1105 6 0.08781679491163175 1105 7 0.17174525962479947 1105 8 0.19120879486657572 1105 9 0.15552342103295302 1105 10-11 0.1596865347985812 1105 12-13 0.1337918625894403 1105 14-15 0.2881929001626702 1105 16-17 0.19357899473287432 1105 18-19 0.19614779836239649 1105 20-21 0.30086047548010697 1105 22-23 0.19316867878305288 1105 24-25 0.31516819097277704 1105 26-27 0.26973291678640976 1105 28-29 0.1898498991290154 1105 30-31 0.33041166114433906 1105 32-33 0.29454263200176456 1105 34-35 0.24786336382265972 1105 36-37 0.3573444234122789 1105 38-39 0.43096982023715924 1105 40-41 0.572527733297548 1105 42-43 0.6167731317427041 1105 44-45 0.7065721312874729 1105 46-47 0.8477519292858133 1105 48-49 0.7708407993442634 1105 50-51 0.5855391486501489 1105 52-53 0.6315001317396352 1105 54-55 0.7648045809933848 1105 56-57 0.7302365165993194 1105 58-59 0.8451042073413575 1105 60-61 1.0507191672423133 1105 62-63 0.9410012624100048 1105 64-65 0.9386262138996955 1105 66-67 1.0991284151178817 1105 68-69 1.1516205504258608 1105 70-71 1.213239719374723 1105 72-73 1.2503624498791197 1105 74-75 1.4022382344503264 1105 76-77 1.4028766187323676 1105 78-79 1.5479194247239256 1105 80-81 1.6864682238309676 1105 82-83 1.954812872357671 1105 84-85 2.0635712390087804 1105 86-87 2.233957641446615 1105 88-89 2.236764941961791 1105 90-91 2.3447305506354574 1105 92-93 2.768933476569451 1105 94-95 3.178534923597187 1105 96-97 3.082007951701616 1105 98-99 3.2216207603819917 1105 100-101 3.298809383885459 1106 1 0.18226996853869792 1106 2 0.09159669013811111 1106 3 0.12192782674678426 1106 4 0.14340474298327166 1106 5 0.15174922546150071 1106 6 -0.07148021227957457 1106 7 0.08839870717133635 1106 8 0.18402089829297097 1106 9 0.18342237407863848 1106 10-11 0.14600474545848385 1106 12-13 0.2778984483550957 1106 14-15 0.45267015005691746 1106 16-17 0.3263749708327026 1106 18-19 0.21277084193261686 1106 20-21 0.08036555162223635 1106 22-23 0.1652928591976064 1106 24-25 0.1560446847333523 1106 26-27 0.22653117979402282 1106 28-29 0.2614942254826573 1106 30-31 0.21444692975686763 1106 32-33 0.380012505626965 1106 34-35 0.3425767072025252 1106 36-37 0.5239482992583007 1106 38-39 0.23307298225068962 1106 40-41 0.6130046791520414 1106 42-43 0.5284280994880461 1106 44-45 0.6127395117866286 1106 46-47 0.7840803847638114 1106 48-49 0.7836980460659149 1106 50-51 0.7481392834851803 1106 52-53 0.590862904053516 1106 54-55 0.3074577245001464 1106 56-57 0.5693895675722445 1106 58-59 0.7978607243675739 1106 60-61 0.9508354954994971 1106 62-63 0.7646135852737856 1106 64-65 0.7736301135401362 1106 66-67 1.1621274369029848 1106 68-69 0.9694445510603771 1106 70-71 1.1907159910540805 1106 72-73 1.4706062104544166 1106 74-75 1.4636625285493103 1106 76-77 1.588877900987022 1106 78-79 1.7280315889582738 1106 80-81 1.8386153394189542 1106 82-83 1.971971290293368 1106 84-85 2.160128649647529 1106 86-87 1.8566244667427227 1106 88-89 2.1615035338611186 1106 90-91 2.3721370914560964 1106 92-93 2.5530305445685997 1106 94-95 2.6773617266851772 1106 96-97 3.0788832821796213 1106 98-99 3.1886886273447708 1106 100-101 3.315961192261092 1107 1 0.23569317438232673 1107 2 0.16783958244982244 1107 3 0.07488909120725395 1107 4 0.006593220047264481 1107 5 0.18137820957949202 1107 6 0.1607023265384413 1107 7 0.16854713219786532 1107 8 0.12244466434848533 1107 9 0.13884701468715122 1107 10-11 0.05628467244419255 1107 12-13 0.09023174641583154 1107 14-15 0.1656152461799465 1107 16-17 0.2734719061624631 1107 18-19 0.17693109209835 1107 20-21 0.2590916220159727 1107 22-23 0.3723368622850032 1107 24-25 0.3594436147286473 1107 26-27 0.27619882631277903 1107 28-29 0.3412001220239702 1107 30-31 0.3401872653237845 1107 32-33 0.32644866325814803 1107 34-35 0.3599568918857159 1107 36-37 0.3756833610527863 1107 38-39 0.40032735268146524 1107 40-41 0.21358702718678302 1107 42-43 0.4253335512833729 1107 44-45 0.40148905661448 1107 46-47 0.49485641049621165 1107 48-49 0.5872109247146895 1107 50-51 0.3794078210520482 1107 52-53 0.4260018832852168 1107 54-55 0.5293819667357695 1107 56-57 0.5141190241355886 1107 58-59 0.562711591295475 1107 60-61 0.596594652629598 1107 62-63 0.7078427243837382 1107 64-65 0.7193862361891874 1107 66-67 0.8300023198031425 1107 68-69 0.8633328339175819 1107 70-71 0.945194768360512 1107 72-73 1.1584310555402695 1107 74-75 1.2644998118951456 1107 76-77 1.3876729396883256 1107 78-79 1.5646483215535802 1107 80-81 1.6910201267407352 1107 82-83 1.6959066542910435 1107 84-85 1.607356884637742 1107 86-87 1.8912718211420945 1107 88-89 2.0868471304836618 1107 90-91 2.1079633417400956 1107 92-93 2.431602284368857 1107 94-95 2.8382045888076917 1107 96-97 2.97493674506714 1107 98-99 2.929919366170875 1107 100-101 2.9162291991828226 1108 1 0.28847142410716486 1108 2 0.1259294526602801 1108 3 0.22717979910321873 1108 4 0.07821606950763993 1108 5 0.11948514375396257 1108 6 0.16422555495179125 1108 7 0.15837302066897507 1108 8 0.13872856143284196 1108 9 0.28034458067151036 1108 10-11 0.2237866854009951 1108 12-13 0.1984889321175558 1108 14-15 0.3615182327429878 1108 16-17 0.3796561157168057 1108 18-19 0.2717282451495464 1108 20-21 0.15808939114275944 1108 22-23 0.16428909795975954 1108 24-25 0.24374421381413924 1108 26-27 0.2835483347081009 1108 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-1.7042924838561966 2316 92-93 -1.983977159626857 2316 94-95 -2.024871083132318 2316 96-97 -2.4599911968516572 2316 98-99 -2.5508630753195654 2316 100-101 -2.43104386245669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3662.0 3 1049.0 4 1006.0 5 1150.0 6 1319.0 7 1584.0 8 2017.0 9 2538.0 10 3182.0 11 4337.0 12 5703.0 13 7668.0 14 8852.0 15 11036.0 16 13718.0 17 17899.0 18 17203.0 19 12877.0 20 13033.0 21 13452.0 22 15008.0 23 16941.0 24 18716.0 25 21360.0 26 24638.0 27 28194.0 28 33150.0 29 39140.0 30 46375.0 31 56759.0 32 70669.0 33 90677.0 34 121992.0 35 176822.0 36 217681.0 37 253090.0 38 147251.0 39 10142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.39505734909677 18.452292357453416 13.836301489771714 22.316348803678103 2 15.581049381426062 20.27658196104656 40.68397088940901 23.458397768118363 3 19.517554029102467 25.485068314835413 29.382792329301083 25.614585326761034 4 12.562189460923431 16.44884706601603 36.83941353631956 34.14954993674098 5 13.222031448858365 38.01120074845878 33.78870673952641 14.978061063156437 6 31.473118911820624 35.7931701325598 17.690493456942566 15.043217498677015 7 28.294399740856335 33.622127002599925 20.437148191904765 17.646325064638972 8 26.563599959772898 34.514003079724105 19.683916865734215 19.23848009476878 9 27.122004611981616 16.737535665795253 20.277241353187538 35.86321836903559 10-11 25.046158026010463 26.850255983562715 26.684694475339825 21.418891515086997 12-13 26.31375978307437 24.49138462503004 27.416282222756294 21.7785733691393 14-15 23.0203978746323 25.521489259370156 26.611567927231008 24.84654493876654 16-17 21.493486372106645 28.81711786046989 27.347408834188442 22.341986933235027 18-19 21.45829462336026 26.98971982850815 30.342457947498993 21.209527600632597 20-21 22.39956604051486 26.05115483581681 30.577847361086754 20.971431762581574 22-23 21.924043142862647 26.1477762890594 30.403903482719308 21.52427708535864 24-25 21.509227151927032 26.434664291576894 30.69636965959986 21.359738896896214 26-27 21.387753475801006 26.58088038385018 30.55723200441015 21.474134135938666 28-29 21.288175420069624 26.881517132427447 30.588794638469256 21.241512809033672 30-31 21.410604514469977 26.719849789787336 30.457569696722313 21.41197599902037 32-33 21.02324436214091 26.71891801695219 30.42352394888023 21.834313672026664 34-35 21.684031434556786 26.8152509523273 30.26622711842821 21.2344904946877 36-37 21.796035202444095 26.482488740044168 30.221483078146854 21.499992979364883 38-39 21.52132726642914 26.714315610699074 30.285929577202687 21.478427545669103 40-41 21.655772097496765 26.691498579494443 30.312302276791925 21.340427046216874 42-43 21.395630061592847 26.80340109003604 30.000574596688622 21.80039425168249 44-45 21.44545550994426 27.090138982967854 29.933095574253464 21.531309932834418 46-47 21.75957500503591 27.091894517827452 29.42192966962792 21.726600807508714 48-49 21.784838513475794 27.171172418251192 29.26763893436883 21.776350133904188 50-51 21.843730617308132 27.490418829367023 29.113407109979434 21.55244344334541 52-53 21.846297481164225 27.456158677513287 29.092128801431127 21.60541503989136 54-55 21.550579873559293 27.512638520997605 29.135781753651507 21.80099985179159 56-57 21.77972760650399 27.655764767868785 28.80335482548333 21.761152800143897 58-59 21.740328335504174 27.32157488745907 28.95698471924344 21.981112057793318 60-61 21.58164712687918 27.38847826661796 29.212365873094377 21.817508733408484 62-63 21.741608575832778 27.321364564950418 29.28006539340177 21.656961465815037 64-65 21.52933091976293 27.33254223068096 29.25087366787073 21.887253181685377 66-67 21.45397201356364 27.536686987756426 29.015167045102896 21.99417395357704 68-69 21.706076449189258 27.36896122176637 29.064186868831953 21.86077546021242 70-71 21.61212268413328 27.336988315548105 28.811974082141123 22.238914918177496 72-73 21.61262047971534 27.331499783728198 28.809199304665757 22.246680431890706 74-75 21.62437254129386 27.640174201502997 28.57398879461661 22.161464462586537 76-77 21.778393926890796 27.380501600798045 28.72893892942387 22.112165542887286 78-79 21.727520612083477 27.373160238730314 28.733751978619647 22.16556717056656 80-81 21.75310101724661 27.614976027244097 28.531774388733083 22.100148566776213 82-83 21.780101601453573 27.673534329500267 28.391704223687725 22.154659845358434 84-85 21.894343332917675 27.459837030430954 28.336446438166295 22.30937319848508 86-87 21.90046267962496 27.421087419520674 28.386300766236626 22.292149134617738 88-89 22.03081439633239 27.362427801714638 28.247204708972063 22.35955309298091 90-91 21.941843430245715 27.42035803174937 28.316008777586266 22.32178976041865 92-93 22.04441940210025 27.306108271721328 28.248177755956405 22.401294570222014 94-95 21.883708424441036 27.166349905167454 28.342671838896006 22.607269831495508 96-97 22.057309716611748 26.890852051815052 28.219594635404643 22.832243596168556 98-99 22.046780093729954 26.969577758153218 27.965740086286406 23.017902061830426 100-101 22.9151766510245 26.1241963721851 28.002890902743548 22.95773607404686 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4672.0 1 3015.0 2 2989.0 3 3734.0 4 2100.5 5 1627.5 6 2724.5 7 4996.0 8 7460.0 9 7815.0 10 5501.5 11 3365.5 12 2904.0 13 2925.5 14 2943.0 15 2960.5 16 2895.5 17 2876.0 18 3057.5 19 3232.5 20 3534.5 21 4128.0 22 4709.5 23 5528.0 24 6733.5 25 8140.5 26 9851.5 27 12076.5 28 14186.5 29 16037.0 30 18175.0 31 20705.5 32 23867.5 33 26062.5 34 27679.5 35 30085.0 36 33329.5 37 36211.0 38 37766.0 39 38948.0 40 40403.5 41 42585.5 42 43718.0 43 44708.0 44 46699.0 45 49148.5 46 51141.5 47 53212.0 48 58187.5 49 58874.0 50 55638.0 51 53527.0 52 51537.0 53 50960.0 54 50305.5 55 47704.0 56 43612.5 57 39647.0 58 36406.0 59 32613.0 60 27635.0 61 23304.5 62 19476.0 63 15060.0 64 10986.0 65 8320.5 66 6392.5 67 4955.0 68 3904.0 69 2963.5 70 2250.5 71 1725.5 72 1322.0 73 1051.0 74 796.0 75 590.5 76 442.0 77 309.5 78 210.0 79 133.0 80 75.0 81 36.0 82 27.0 83 20.0 84 16.0 85 8.0 86 3.0 87 6.5 88 7.0 89 7.0 90 5.0 91 2.5 92 3.0 93 2.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4410891121425168 2 0.040864552937874124 3 0.05333281110262487 4 0.05764121444751255 5 0.08342635567827977 6 0.0822513365842195 7 0.04523823512132072 8 0.03812284171840015 9 0.04314931228743578 10-11 0.04811050401791251 12-13 0.04399793718870154 14-15 0.03776380810632617 16-17 0.039199942554622065 18-19 0.04820842227575087 20-21 0.05689050780408515 22-23 0.048600095307104296 24-25 0.06531147797818382 26-27 0.057184262577600224 28-29 0.04474864383212894 30-31 0.045858384087630315 32-33 0.04579310524907141 34-35 0.05042790278675361 36-37 0.04500975918636455 38-39 0.026862242066989145 40-41 0.025654583553649413 42-43 0.024871237490942562 44-45 0.014850935772150744 46-47 0.02542610761869325 48-49 0.025099713425898725 50-51 0.01566692125413705 52-53 0.015014132868548002 54-55 0.017168334540991845 56-57 0.01602595486621102 58-59 0.014687738675753481 60-61 0.017723204668742533 62-63 0.015471084738460334 64-65 0.017788483507301438 66-67 0.009661268106717845 68-69 0.013447440743134299 70-71 0.01840863247361103 72-73 0.01648290673612335 74-75 0.01406758970944389 76-77 0.01005294113807127 78-79 0.013284243646737037 80-81 0.016678743251800063 82-83 0.014296065644400056 84-85 0.012598815841868543 86-87 0.003427139024342479 88-89 0.028102539999608326 90-91 0.031953991474583686 92-93 0.017103055702432944 94-95 0.03306373173008506 96-97 0.02640529019707681 98-99 0.02366357897760283 100-101 0.14779129049735945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1531890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.054391062323376 #Duplication Level Percentage of deduplicated Percentage of total 1 79.36666548860453 48.456834320538654 2 12.537830419330954 15.309792029898519 3 3.7525873368714056 6.873358042826084 4 1.5304300229570662 3.7375789246056508 5 0.7655934856584681 2.3371422034079687 6 0.44922257729577697 1.6456206544944694 7 0.2911394048385619 1.244273735366595 8 0.221077540801965 1.079820370497594 9 0.17058410327468146 0.9373417695313344 >10 0.8369880488714275 9.173965543569233 >50 0.049981269663982066 2.1021168900987868 >100 0.024788458415368895 2.9693118199318898 >500 0.0016095741806127876 0.6618262766394318 >1k 0.0011803543991160445 1.5298263102165286 >5k 1.0730494537418586E-4 0.4366533492181561 >10k+ 2.1460989074837173E-4 1.504537759159033 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12935 0.8443817767594279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10030 0.6547467507458107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6665 0.4350834589950976 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4665 0.30452578187728885 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4373 0.28546436101808875 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2322 0.15157746313377593 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2219 0.14485374276220878 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 0.1334299460144005 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1783 0.11639216915052648 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1564 0.10209610350612641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.527883855890436E-5 0.0 14-15 0.0 0.0 0.0 6.527883855890436E-5 0.0 16-17 0.0 0.0 0.0 1.631970963972609E-4 0.0 18-19 0.0 0.0 0.0 1.9583651567671307E-4 0.0 20-21 0.0 0.0 0.0 3.59033612073974E-4 0.0 22-23 0.0 0.0 0.0 5.54870127750687E-4 0.0 24-25 0.0 0.0 0.0 0.0011423796747808263 0.0 26-27 0.0 0.0 0.0 0.0028069900580328876 0.0 28-29 0.0 0.0 0.0 0.006462605017331532 0.0 30-31 0.0 0.0 0.0 0.017396810475948012 0.0 32-33 0.0 0.0 0.0 0.03910202429678371 6.527883855890436E-5 34-35 0.0 0.0 0.0 0.07092545809424959 6.527883855890436E-5 36-37 0.0 3.263941927945218E-5 0.0 0.12207142810515116 6.527883855890436E-5 38-39 0.0 6.527883855890436E-5 0.0 0.1975337654792446 6.527883855890436E-5 40-41 0.0 6.527883855890436E-5 0.0 0.27756562155246134 6.527883855890436E-5 42-43 0.0 6.527883855890436E-5 0.0 0.354921045244763 6.527883855890436E-5 44-45 0.0 6.527883855890436E-5 0.0 0.45071774082995514 6.527883855890436E-5 46-47 0.0 6.527883855890436E-5 0.0 0.5668161552069666 6.527883855890436E-5 48-49 0.0 6.527883855890436E-5 0.0 0.6883327131843671 6.527883855890436E-5 50-51 0.0 6.527883855890436E-5 0.0 0.8118076363185347 6.527883855890436E-5 52-53 0.0 6.527883855890436E-5 0.0 0.9270900652135597 1.305576771178087E-4 54-55 0.0 6.527883855890436E-5 0.0 1.0485413443524014 1.305576771178087E-4 56-57 0.0 6.527883855890436E-5 0.0 1.1928728564061388 1.305576771178087E-4 58-59 0.0 6.527883855890436E-5 0.0 1.3504886121066133 1.305576771178087E-4 60-61 0.0 6.527883855890436E-5 0.0 1.5211601355188689 1.305576771178087E-4 62-63 6.527883855890436E-5 6.527883855890436E-5 0.0 1.687555895005516 1.305576771178087E-4 64-65 6.527883855890436E-5 6.527883855890436E-5 0.0 1.8476848859905086 1.305576771178087E-4 66-67 6.527883855890436E-5 6.527883855890436E-5 0.0 2.027430167962452 1.305576771178087E-4 68-69 6.527883855890436E-5 6.527883855890436E-5 0.0 2.231263341362631 1.305576771178087E-4 70-71 6.527883855890436E-5 6.527883855890436E-5 0.0 2.453603065494259 1.305576771178087E-4 72-73 6.527883855890436E-5 6.527883855890436E-5 0.0 2.671830222796676 1.305576771178087E-4 74-75 6.527883855890436E-5 6.527883855890436E-5 0.0 2.886891356428986 1.305576771178087E-4 76-77 6.527883855890436E-5 6.527883855890436E-5 0.0 3.1154978490622693 1.305576771178087E-4 78-79 6.527883855890436E-5 6.527883855890436E-5 0.0 3.369693646410643 1.305576771178087E-4 80-81 6.527883855890436E-5 6.527883855890436E-5 0.0 3.6347257309597945 1.305576771178087E-4 82-83 6.527883855890436E-5 6.527883855890436E-5 0.0 3.9083419827794423 1.9583651567671307E-4 84-85 6.527883855890436E-5 6.527883855890436E-5 0.0 4.1852548159463145 1.9583651567671307E-4 86-87 6.527883855890436E-5 6.527883855890436E-5 0.0 4.470719176964403 2.611153542356174E-4 88-89 6.527883855890436E-5 6.527883855890436E-5 0.0 4.775244958841692 3.263941927945218E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7040 0.0 33.535236 1 GTATCAA 10605 0.0 31.931293 1 ATCAACG 13050 0.0 25.78772 3 TATCAAC 13095 0.0 25.698265 2 TCAACGC 13135 0.0 25.620842 4 CAACGCA 13240 0.0 25.381754 5 GTACATG 9380 0.0 24.813372 1 AACGCAG 13640 0.0 24.737925 6 TACATGG 9170 0.0 24.672527 2 GAGTACT 8045 0.0 23.29501 12-13 ACATGGG 9420 0.0 23.261635 3 ACGCAGA 14850 0.0 22.618841 7 CGCAGAG 14985 0.0 22.351658 8 TACCTGG 1245 0.0 21.761135 2 GTACTTT 8785 0.0 21.198961 14-15 GCAGAGT 15700 0.0 21.09027 9 AGTACTT 8365 0.0 21.040895 12-13 CATGGGG 3415 0.0 21.017279 4 GTACCTG 1330 0.0 20.79913 1 GTGTAGC 1190 0.0 20.440525 1 >>END_MODULE