FastQCFastQC Report
Thu 26 May 2016
SRR937926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531890
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123040.8031908296287592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101680.6637552304669395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70540.4604769271945114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46260.3019799071734916No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41880.27338777588469143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.16208735614175954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.14857463656006634No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.13127574434195666No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17130.11182265045140316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73650.034.3149871
GTATCAA107000.029.9240971
TACATGG85350.025.2138772
GTACATG87450.025.2059541
ATCAACG130500.024.3502713
TATCAAC131350.024.3043582
TCAACGC131450.024.2811134
CAACGCA133000.023.9267145
ACATGGG88250.023.5747783
AACGCAG136400.023.4347636
GAGTACT85250.022.7592312-13
ACGCAGA151800.021.0260357
CGCAGAG152300.020.9570068
GTACTTT91850.020.89113814-15
TACCTGG11450.020.744812
CATGGGA55650.020.739634
AATACCG1150.00372551120.650575
AGTACTT89200.020.52671212-13
GCAGAGT160350.019.8456679
CATGGGG31100.019.548324
Produced by FastQC (version 0.11.5)