##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937926_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38636651456697 33.0 31.0 34.0 31.0 34.0 2 32.61905097624503 34.0 31.0 34.0 31.0 34.0 3 32.579498527962194 34.0 31.0 34.0 31.0 34.0 4 36.1389877863293 37.0 35.0 37.0 35.0 37.0 5 36.10367454582248 37.0 35.0 37.0 35.0 37.0 6 36.17248823348935 37.0 36.0 37.0 35.0 37.0 7 36.12779311830484 37.0 36.0 37.0 35.0 37.0 8 36.132032326080854 37.0 36.0 37.0 35.0 37.0 9 37.80540900456299 39.0 38.0 39.0 35.0 39.0 10-11 37.76560490635751 39.0 38.0 39.0 35.0 39.0 12-13 37.702159750373724 39.0 37.0 39.0 35.0 39.0 14-15 38.90350482084223 40.0 38.0 41.0 35.0 41.0 16-17 38.83355593417282 40.0 38.0 41.0 35.0 41.0 18-19 38.84170273322497 40.0 38.0 41.0 35.0 41.0 20-21 38.84982178877073 40.0 38.0 41.0 35.0 41.0 22-23 38.85166624235421 40.0 38.0 41.0 35.0 41.0 24-25 38.78184171187226 40.0 38.0 41.0 35.0 41.0 26-27 38.58475934956165 40.0 38.0 41.0 34.0 41.0 28-29 38.488614717766936 40.0 38.0 41.0 34.0 41.0 30-31 38.23463923649871 40.0 37.5 41.0 34.0 41.0 32-33 37.98500447160044 40.0 37.0 41.0 33.5 41.0 34-35 37.754253895514694 40.0 37.0 41.0 33.0 41.0 36-37 37.64635482965487 40.0 37.0 41.0 33.0 41.0 38-39 37.57898772105047 40.0 37.0 41.0 33.0 41.0 40-41 37.42902786753618 40.0 36.0 41.0 33.0 41.0 42-43 37.211091527459544 40.0 36.0 41.0 32.5 41.0 44-45 36.91475040636077 40.0 36.0 41.0 31.0 41.0 46-47 36.49466312855361 39.0 35.0 41.0 30.5 41.0 48-49 36.07342008890978 39.0 35.0 41.0 29.5 41.0 50-51 35.747891819908745 39.0 35.0 40.0 28.5 41.0 52-53 35.25075560255632 38.0 35.0 40.0 27.0 41.0 54-55 34.922111574590865 38.0 34.0 40.0 26.0 41.0 56-57 34.64185450652462 38.0 34.0 40.0 26.0 41.0 58-59 34.24983223338491 37.5 33.5 40.0 25.0 41.0 60-61 33.8102602014505 37.0 33.0 40.0 23.5 41.0 62-63 33.3568764728538 36.0 33.0 39.0 22.5 41.0 64-65 32.89294433673436 36.0 32.0 39.0 21.5 40.0 66-67 32.3622123651176 35.0 31.5 38.5 19.5 40.0 68-69 31.807951288930667 35.0 31.0 38.0 18.0 40.0 70-71 31.19487952790344 35.0 30.5 37.5 14.5 39.5 72-73 30.696717453603064 34.0 30.0 37.0 10.0 39.0 74-75 30.117384733890816 34.0 29.0 36.0 7.5 39.0 76-77 28.95689670929375 32.5 28.0 35.0 7.0 37.0 78-79 29.6707645457572 34.0 29.0 35.0 6.0 37.0 80-81 29.474170795553206 34.0 29.0 35.0 2.0 37.0 82-83 28.989401327771574 34.0 29.0 35.0 2.0 36.5 84-85 28.44383408730392 34.0 28.5 35.0 2.0 36.0 86-87 27.842467801212884 33.0 26.5 35.0 2.0 36.0 88-89 27.14678175325905 33.0 25.5 35.0 2.0 35.0 90-91 26.54709052216543 32.0 25.0 35.0 2.0 35.0 92-93 25.843219487038887 32.0 23.5 34.0 2.0 35.0 94-95 25.01944917716024 31.5 19.5 34.0 2.0 35.0 96-97 24.099168673990953 31.0 9.0 34.0 2.0 35.0 98-99 22.97890285856034 31.0 2.0 34.0 2.0 35.0 100-101 19.58793124832723 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08073373416434748 1101 2 0.002067109364844555 1101 3 0.13806305261523732 1101 4 0.052019226413143826 1101 5 0.048358933282486305 1101 6 0.057104361396113745 1101 7 0.10252611669007194 1101 8 0.0750633228161206 1101 9 0.021232289361428514 1101 10-11 0.08304084737803663 1101 12-13 0.07275537257604014 1101 14-15 0.1697942299753663 1101 16-17 0.15134134302245172 1101 18-19 0.2585423089905703 1101 20-21 0.2776187439721909 1101 22-23 0.07379327119544854 1101 24-25 0.15914173897866846 1101 26-27 0.3560488883752271 1101 28-29 0.19714669924932338 1101 30-31 0.28058842680112406 1101 32-33 0.2673981204488953 1101 34-35 0.3159471828288858 1101 36-37 0.4221171427812678 1101 38-39 0.44844549969804604 1101 40-41 0.5486255634834905 1101 42-43 0.48690954801531916 1101 44-45 0.5734562783201653 1101 46-47 0.672098311343639 1101 48-49 0.668451721317993 1101 50-51 0.6726074444768528 1101 52-53 0.7851096518784857 1101 54-55 0.6641374182437048 1101 56-57 0.6850024045569896 1101 58-59 0.8343336732950704 1101 60-61 0.8026153551697419 1101 62-63 0.7866917600648122 1101 64-65 0.6573767369784136 1101 66-67 0.6128429162555804 1101 68-69 0.8980279353720277 1101 70-71 0.9738974987126028 1101 72-73 1.0074021899548882 1101 74-75 0.9117690904259774 1101 76-77 1.0067169384252956 1101 78-79 0.9519510577554762 1101 80-81 1.1438969761350712 1101 82-83 1.3114733336262958 1101 84-85 1.4623353792087102 1101 86-87 1.453574425068922 1101 88-89 1.9667566946288098 1101 90-91 1.9488681283227187 1101 92-93 2.1892439495392857 1101 94-95 2.141587560486684 1101 96-97 2.5334043752730615 1101 98-99 2.5947951187400555 1101 100-101 2.7836553168688773 1102 1 0.10269140506498786 1102 2 0.12070965342602591 1102 3 0.22172135921152858 1102 4 0.10056044274545428 1102 5 0.10412913989355843 1102 6 0.10759641619939231 1102 7 0.08881630715903555 1102 8 0.07698519338353549 1102 9 0.1340482892335757 1102 10-11 0.18520514933147325 1102 12-13 0.1794716309617499 1102 14-15 0.30518471731539876 1102 16-17 0.2803798683364036 1102 18-19 0.3588986142043922 1102 20-21 0.24773185864113145 1102 22-23 0.24714619615459554 1102 24-25 0.26121963666803083 1102 26-27 0.2941499723741572 1102 28-29 0.1184004500933824 1102 30-31 0.21098504734556656 1102 32-33 0.17595244226033202 1102 34-35 0.18649211903956342 1102 36-37 0.2739677922616508 1102 38-39 0.32724279229784514 1102 40-41 0.2456667418578533 1102 42-43 0.11433336242895109 1102 44-45 -0.05670600187337982 1102 46-47 0.06478920982812753 1102 48-49 -0.04882303824840761 1102 50-51 -0.07648506102705 1102 52-53 0.01401352638553277 1102 54-55 -0.03744023371791627 1102 56-57 -0.022559117276820473 1102 58-59 -0.04804415207441792 1102 60-61 0.10317433581917612 1102 62-63 0.1026097229619296 1102 64-65 0.14012934708641467 1102 66-67 0.04487322667164051 1102 68-69 0.12094144720091649 1102 70-71 0.2974275274407674 1102 72-73 0.30749968193377697 1102 74-75 0.3261244846491813 1102 76-77 0.3864891508139685 1102 78-79 0.49570557774732293 1102 80-81 0.7010671316028088 1102 82-83 0.6679168941612232 1102 84-85 0.649178856635718 1102 86-87 0.8989879069310156 1102 88-89 1.0048709640183269 1102 90-91 1.1514162050536996 1102 92-93 1.33520971946621 1102 94-95 1.5417821448871685 1102 96-97 1.96335598888518 1102 98-99 2.2662221775588876 1102 100-101 2.668094857857458 1103 1 0.2280752869218361 1103 2 0.24176916130791426 1103 3 0.3399007711788542 1103 4 0.19573474112242195 1103 5 0.17901767667672175 1103 6 0.15415595248340708 1103 7 0.13236401003175757 1103 8 0.18222169844817415 1103 9 0.14999456934133093 1103 10-11 0.20173548581804113 1103 12-13 0.2500910872605502 1103 14-15 0.3262482135802145 1103 16-17 0.36081376814097865 1103 18-19 0.455945337506094 1103 20-21 0.41250917997064107 1103 22-23 0.4466810661031033 1103 24-25 0.3510322793584706 1103 26-27 0.4839126998479557 1103 28-29 0.40662033810442466 1103 30-31 0.41903602129659134 1103 32-33 0.4042366036665541 1103 34-35 0.4591896437040859 1103 36-37 0.3887075261035591 1103 38-39 0.652320475119879 1103 40-41 0.6149529300877745 1103 42-43 0.7277968137338178 1103 44-45 0.763141762758849 1103 46-47 0.7813892221558518 1103 48-49 0.7422769456771974 1103 50-51 0.8643708459493595 1103 52-53 0.8188864238732165 1103 54-55 1.0657501100444406 1103 56-57 0.9937639441575712 1103 58-59 0.9440709357364767 1103 60-61 0.9037014066661584 1103 62-63 1.1067346777904703 1103 64-65 1.2007808675667633 1103 66-67 1.229502421876397 1103 68-69 1.0908466808053419 1103 70-71 1.4365414175281899 1103 72-73 1.193938938257478 1103 74-75 1.1012567358012788 1103 76-77 1.0635343754505762 1103 78-79 1.4956874487340137 1103 80-81 1.5479981313695497 1103 82-83 1.6394428615555903 1103 84-85 1.6177491905600583 1103 86-87 1.837236559643177 1103 88-89 2.1003930373006305 1103 90-91 1.978612867381493 1103 92-93 2.1804652962528515 1103 94-95 2.2853892504935054 1103 96-97 2.5636828549951645 1103 98-99 2.963264912477662 1103 100-101 3.0493449862713042 1104 1 0.17856002237557078 1104 2 0.19288900941853626 1104 3 0.25571474029692354 1104 4 0.13438420262081507 1104 5 0.11303148785672334 1104 6 0.056726646570808725 1104 7 0.05590518314195947 1104 8 0.10377520416923147 1104 9 0.2010606513011055 1104 10-11 0.1690473462948816 1104 12-13 0.12179581028086517 1104 14-15 0.3174062020540944 1104 16-17 0.23502785823287553 1104 18-19 0.32216087256330894 1104 20-21 0.2404953015829534 1104 22-23 0.3020181966708364 1104 24-25 0.2064273765575919 1104 26-27 0.24197762247509758 1104 28-29 0.32599883500447646 1104 30-31 0.26432308213635025 1104 32-33 0.20638976998878178 1104 34-35 0.2527729896388635 1104 36-37 0.24608861049961206 1104 38-39 0.3192383305225377 1104 40-41 0.35229856242811053 1104 42-43 0.2916004328678454 1104 44-45 0.3457576540168077 1104 46-47 0.2885048361820921 1104 48-49 0.1845430209162089 1104 50-51 0.23315707509397754 1104 52-53 0.23305212442936352 1104 54-55 0.24814841641067886 1104 56-57 0.42835490136307897 1104 58-59 0.3833546697956578 1104 60-61 0.4768779780659287 1104 62-63 0.47020561886613166 1104 64-65 0.4341847689730898 1104 66-67 0.39574484000903 1104 68-69 0.6436467211299544 1104 70-71 0.7217557260450818 1104 72-73 0.6294624021417476 1104 74-75 0.660309611450252 1104 76-77 0.6085694074582335 1104 78-79 0.7733215700774956 1104 80-81 0.8825613654035216 1104 82-83 1.1532557217467954 1104 84-85 1.2719647928249564 1104 86-87 1.4101501809614554 1104 88-89 1.4149519028348472 1104 90-91 1.3794949682938338 1104 92-93 1.5286313664512825 1104 94-95 1.860450157461262 1104 96-97 2.0564135033813393 1104 98-99 2.4469586470408977 1104 100-101 2.5797295822690884 1105 1 -7.700429839019307E-4 1105 2 0.045161272490432225 1105 3 0.05774701153875128 1105 4 -0.05224518871905559 1105 5 -0.03820932096680707 1105 6 0.013497479904138743 1105 7 -0.02883491081228584 1105 8 -0.04951064141900474 1105 9 0.043439634144242234 1105 10-11 0.033534938206649656 1105 12-13 0.039783708565174436 1105 14-15 -0.1418398661648581 1105 16-17 -0.036473111946861536 1105 18-19 0.03532723285742634 1105 20-21 0.050614531566615995 1105 22-23 0.10439130737345437 1105 24-25 0.11297864289746684 1105 26-27 0.11626913595549837 1105 28-29 0.12682221122996395 1105 30-31 0.16171891546968453 1105 32-33 0.012181487717548123 1105 34-35 0.06417387811925579 1105 36-37 -0.04070800918992745 1105 38-39 0.004726484117306029 1105 40-41 0.11081571929086209 1105 42-43 0.10726372943647533 1105 44-45 0.14705718424556125 1105 46-47 0.0379431531755543 1105 48-49 0.041839793792028956 1105 50-51 0.21227395622142353 1105 52-53 0.31631907704374385 1105 54-55 0.15059556714597733 1105 56-57 0.33832732783366026 1105 58-59 0.33549927763879595 1105 60-61 0.28589299032082494 1105 62-63 0.45547292891514246 1105 64-65 0.4318295300024957 1105 66-67 0.5545479445841934 1105 68-69 0.534022793433877 1105 70-71 0.8417286631365783 1105 72-73 0.6426823776319424 1105 74-75 0.5402345501757466 1105 76-77 0.5404742787817618 1105 78-79 0.6047770949957965 1105 80-81 0.7739254891780369 1105 82-83 0.8751868900474484 1105 84-85 1.0659662736256124 1105 86-87 1.2619450134777956 1105 88-89 1.3442206936845231 1105 90-91 1.349631304078148 1105 92-93 1.8459771589349465 1105 94-95 1.7410899654678005 1105 96-97 1.8575419756035636 1105 98-99 2.192382207089917 1105 100-101 2.5334137121710505 1106 1 0.05453875566245614 1106 2 0.07319241673747001 1106 3 0.20436027296007353 1106 4 0.10281839524879643 1106 5 0.01721184020070865 1106 6 0.06704813557249878 1106 7 0.04327869430615294 1106 8 -0.03207131718042433 1106 9 -0.050088945593486756 1106 10-11 0.09367036809243956 1106 12-13 0.033529412351136045 1106 14-15 0.155446578949352 1106 16-17 0.12841062589154717 1106 18-19 0.14814250887265246 1106 20-21 0.12467299973497603 1106 22-23 0.13508445193775032 1106 24-25 0.0545963988196263 1106 26-27 0.022110995621311247 1106 28-29 -0.07300957546631537 1106 30-31 0.010346770799635863 1106 32-33 0.061404533931586514 1106 34-35 -0.04224169136043798 1106 36-37 -0.12974704525169045 1106 38-39 -0.04139502603498357 1106 40-41 0.027614577158878717 1106 42-43 -0.1913198418410218 1106 44-45 0.035438238602587546 1106 46-47 -0.34079785782274996 1106 48-49 -0.11524962033657715 1106 50-51 0.03640182477049336 1106 52-53 0.0644089141841846 1106 54-55 0.14041849703176723 1106 56-57 0.3055769576982996 1106 58-59 0.3844893368604474 1106 60-61 0.4072676466237013 1106 62-63 0.3811666022442495 1106 64-65 0.5044669427563022 1106 66-67 0.4851475638735323 1106 68-69 0.16388908203370534 1106 70-71 0.3487447111484272 1106 72-73 0.39670759971316016 1106 74-75 0.6550194750911444 1106 76-77 0.5898162970770251 1106 78-79 1.0330848493316687 1106 80-81 0.9744843274817576 1106 82-83 1.0018987661049792 1106 84-85 0.9989073295689259 1106 86-87 1.0674487941461557 1106 88-89 1.3873943400466189 1106 90-91 1.3747311084351708 1106 92-93 1.1792642253478043 1106 94-95 1.7205904149178828 1106 96-97 1.9663683821179454 1106 98-99 2.1646666693361105 1106 100-101 2.4349015241422833 1107 1 0.08101771503775979 1107 2 0.06434923871769627 1107 3 0.13708388308995012 1107 4 0.025868235432795927 1107 5 0.019234827561703582 1107 6 -0.027618336057436466 1107 7 0.03779367530563604 1107 8 0.020783896058006235 1107 9 0.04178269119002209 1107 10-11 0.031672224572375285 1107 12-13 -0.00553194485552666 1107 14-15 0.026725838349243247 1107 16-17 0.12414756906427726 1107 18-19 0.08450263330193764 1107 20-21 0.05591088126261212 1107 22-23 0.06642192668930846 1107 24-25 0.08924336418296974 1107 26-27 0.08339272929035957 1107 28-29 0.055258521711770925 1107 30-31 0.013444275678146766 1107 32-33 -0.014302655765497718 1107 34-35 0.06198903657266186 1107 36-37 0.043198727794028 1107 38-39 0.11531335566849776 1107 40-41 0.2463633127917788 1107 42-43 0.1561764778781196 1107 44-45 0.12522872713847022 1107 46-47 0.139604646635604 1107 48-49 0.32005213157048473 1107 50-51 0.24248432530736608 1107 52-53 0.058793179077255786 1107 54-55 0.17146143260428204 1107 56-57 0.07877206393626324 1107 58-59 0.25464078080658226 1107 60-61 0.38080736097789014 1107 62-63 0.3320418555067022 1107 64-65 0.32158694014197664 1107 66-67 0.4941076472206092 1107 68-69 0.5994900026485652 1107 70-71 0.7439426838038656 1107 72-73 0.5550085004093788 1107 74-75 0.6259273606958757 1107 76-77 0.6352395604979897 1107 78-79 0.7141231553754217 1107 80-81 0.7950041107420027 1107 82-83 1.0638947699268684 1107 84-85 1.1586614743588015 1107 86-87 1.3134858786167705 1107 88-89 1.2547274124664547 1107 90-91 1.2073174277497216 1107 92-93 1.3443637252030811 1107 94-95 1.447915644371335 1107 96-97 1.1785641948453325 1107 98-99 1.4732747528823786 1107 100-101 1.7935801010173584 1108 1 0.04255325018878864 1108 2 0.09326305461895146 1108 3 0.11955047539417052 1108 4 0.028946766602231833 1108 5 -0.047989064942711934 1108 6 0.025802951791689566 1108 7 0.03834474531220877 1108 8 0.048140902255696005 1108 9 -0.09429725089591301 1108 10-11 0.009497381730746213 1108 12-13 0.01565359435834779 1108 14-15 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-3.8752385839506935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 13.0 5 38.0 6 92.0 7 208.0 8 463.0 9 780.0 10 1323.0 11 2377.0 12 3637.0 13 5199.0 14 7142.0 15 9082.0 16 12182.0 17 17087.0 18 16924.0 19 12311.0 20 11019.0 21 11622.0 22 12059.0 23 13220.0 24 14901.0 25 17363.0 26 20088.0 27 24451.0 28 29613.0 29 35651.0 30 43173.0 31 52933.0 32 66418.0 33 86180.0 34 118617.0 35 180038.0 36 238693.0 37 295206.0 38 163450.0 39 8320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56906174725339 18.993400309421695 14.338235774109107 22.099302169215807 2 15.922127267860523 20.59201034124813 39.973624594412854 23.51223779647849 3 19.931870912483237 25.42241209350058 29.513943744540725 25.131773249475454 4 12.832579362747978 16.853233587268015 36.540221556378064 33.77396549360594 5 13.163951933822837 38.21114859571979 33.830475857242824 14.794423613214544 6 31.572584382911 35.99974149512431 17.403775476765244 15.023898645199447 7 28.194583161976382 33.6156643101006 20.34604312320075 17.843709404722272 8 26.574397083859918 34.7766285785906 19.500120113219765 19.148854224329718 9 26.83397632989314 16.725482900208238 20.240878914282355 36.19966185561626 10-11 25.110974025550135 26.97755713530345 26.54390981075665 21.367559028389767 12-13 26.22639354000614 24.51429280170247 27.482162557363782 21.777151100927615 14-15 22.9064097291581 25.61009602517152 26.591432805227527 24.892061440442852 16-17 21.45415841025087 28.952447288136646 27.31254441002435 22.280849891588133 18-19 21.418378604207874 26.990612903015233 30.346891748102017 21.24411674467488 20-21 22.24621219539262 26.005816344515598 30.665746235042985 21.082225225048795 22-23 21.87836594011319 26.190979770087928 30.49572750001632 21.43492678978256 24-25 21.53501977657271 26.40748552579441 30.765871360559004 21.291623337073876 26-27 21.360812854769428 26.559695940133484 30.603206379459113 21.476284825637975 28-29 21.21650975189024 26.876049566464793 30.670400634513417 21.237040047131554 30-31 21.388567118298756 26.706023316971176 30.51024927728095 21.39516028744912 32-33 21.05098923968866 26.742177508905996 30.509722665590832 21.69711058581451 34-35 21.615442899212898 26.797374952623986 30.335991830922556 21.25119031724056 36-37 21.728582694848775 26.56826953710522 30.273895513111782 21.42925225493422 38-39 21.49144614267605 26.671032584086625 30.35110268252544 21.486418590711892 40-41 21.65790415298748 26.647803103314082 30.357800465418762 21.336492278279675 42-43 21.363317326055515 26.76072982740215 30.11279091404712 21.76316193249521 44-45 21.47662157830379 26.972749167119858 29.998472318609053 21.5521569359673 46-47 21.717024506586302 27.161115602764884 29.38250948932127 21.739350401327542 48-49 21.676272466572275 27.224756134101273 29.342960675297853 21.7560107240286 50-51 21.827024133586615 27.462840021150342 29.190640320127425 21.519495525135618 52-53 21.936757861204136 27.428242236714123 29.045035870721787 21.589964031359955 54-55 21.566137255285952 27.48882099889679 29.07525997297456 21.869781772842696 56-57 21.711480589337352 27.684885990508455 28.803177773861048 21.80045564629314 58-59 21.664969416864135 27.358589715971775 28.923649870421503 22.052790996742587 60-61 21.597764852567742 27.42432550640059 29.12105307822363 21.856856562808037 62-63 21.69033024564427 27.384897087911014 29.1790859657025 21.74568670074222 64-65 21.471907251826174 27.38029492979261 29.20993021692158 21.937867601459633 66-67 21.457464374808445 27.60204492956887 28.89685365071107 22.04363704491162 68-69 21.672036407397023 27.461238153087713 28.933148767875426 21.933576671639837 70-71 21.517765635913804 27.511994986585197 28.65228573853214 22.317953638968856 72-73 21.557879482208254 27.53670302697974 28.57411432935785 22.33130316145415 74-75 21.512510689409815 27.775590936686058 28.33999177486634 22.37190659903779 76-77 21.556410708340675 27.467899131138658 28.73933506975044 22.236355090770225 78-79 21.45973274843494 27.500277435063875 28.785585126869424 22.254404689631762 80-81 21.473135936460128 27.642122778518146 28.67953509671233 22.205206188309397 82-83 21.489274408102236 27.670006694416998 28.53766972272841 22.303049174752353 84-85 21.560250954658493 27.51352058860298 28.423673506957854 22.50255494978067 86-87 21.521223778517683 27.541740418708144 28.42474125722112 22.512294545553058 88-89 21.551587108600195 27.492909899832853 28.352860960271613 22.60264203129534 90-91 21.496466167548668 27.4794519385404 28.513962497931406 22.51011939597953 92-93 21.439978954013146 27.458941884288198 28.4596380860555 22.641441075643154 94-95 21.25812599407464 27.32925579814993 28.535804965044804 22.876813242730627 96-97 21.399475679684155 27.05661249386378 28.43876314717525 23.105148679276812 98-99 21.25481064711955 27.132457747414385 28.289297817693647 23.323433787772412 100-101 21.610078347032015 26.793131260247083 28.45681839175605 23.139972000964853 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4515.0 1 2905.5 2 2892.5 3 3708.5 4 2214.0 5 1733.0 6 2893.0 7 5322.0 8 7466.0 9 7644.5 10 5513.0 11 3383.0 12 2980.0 13 2954.5 14 2866.0 15 2893.0 16 2940.5 17 3064.5 18 3205.0 19 3300.5 20 3539.0 21 3980.5 22 4629.5 23 5495.5 24 6669.0 25 8101.5 26 9835.5 27 12041.0 28 14072.0 29 15966.0 30 18149.5 31 20736.5 32 23977.5 33 26184.5 34 27673.0 35 29920.0 36 33092.5 37 36053.0 38 37917.0 39 39401.5 40 40916.0 41 42938.5 42 43868.0 43 44899.5 44 46983.0 45 49414.0 46 51410.5 47 53561.0 48 59044.5 49 59721.0 50 56010.0 51 53789.5 52 51644.5 53 51005.5 54 50637.0 55 47715.5 56 43369.0 57 39496.0 58 36272.0 59 32609.5 60 27468.5 61 22918.0 62 18965.5 63 14554.0 64 10482.5 65 7772.5 66 5828.5 67 4628.5 68 3661.5 69 2782.0 70 2091.0 71 1480.5 72 1165.0 73 968.0 74 748.5 75 522.0 76 355.5 77 249.5 78 166.0 79 89.5 80 49.5 81 24.0 82 14.5 83 9.0 84 6.5 85 5.5 86 3.0 87 1.5 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010444614169424698 3 0.006005653147419201 4 0.0 5 1.305576771178087E-4 6 2.611153542356174E-4 7 0.0 8 1.305576771178087E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.263941927945218E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017625286410904177 26-27 0.022814954076337074 28-29 4.895912891917828E-4 30-31 9.791825783835653E-5 32-33 0.0037208937978575485 34-35 0.017005137444594583 36-37 0.009008479721128802 38-39 0.021509377305158985 40-41 0.023892054912558996 42-43 0.019257257374876786 44-45 0.010248777653747985 46-47 0.0023826776074000093 48-49 1.9583651567671307E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2847593495616526E-4 68-69 2.2847593495616526E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.791825783835653E-5 82-83 0.0010771008362219219 84-85 0.0038840908942548093 86-87 7.507066434274001E-4 88-89 3.263941927945218E-5 90-91 0.0035576967014602874 92-93 4.2431245063287833E-4 94-95 3.263941927945218E-5 96-97 1.305576771178087E-4 98-99 9.791825783835653E-5 100-101 0.0033618601857835747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1531890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.06201341812225 #Duplication Level Percentage of deduplicated Percentage of total 1 78.48093240412915 47.137228151235504 2 13.096988360641118 15.732629813076354 3 3.842227499385685 6.923157588707439 4 1.635537034335248 3.929345892083181 5 0.8068762700219125 2.423130667841028 6 0.48521151682458113 1.7485668380487263 7 0.3138280224418709 1.3194400026420494 8 0.2165869581021902 1.0406919026979222 9 0.15640347900911863 0.8454517069398015 >10 0.8829560364824012 9.50455712320568 >50 0.05314553386137999 2.2006797085162813 >100 0.026147506509868978 3.085930423781323 >500 0.0017431052444132102 0.731813929948227 >1k 0.0010894407777582563 1.4453717720517156 >5k 1.0894407777582563E-4 0.4615714826408077 >10k+ 2.1788815555165127E-4 1.4704329965840983 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12304 0.8031908296287592 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10168 0.6637552304669395 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7054 0.4604769271945114 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4626 0.3019799071734916 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4188 0.27338777588469143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2483 0.16208735614175954 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2276 0.14857463656006634 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2011 0.13127574434195666 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1713 0.11182265045140316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.527883855890436E-5 0.0 8 0.0 0.0 0.0 6.527883855890436E-5 0.0 9 0.0 0.0 0.0 6.527883855890436E-5 0.0 10-11 0.0 0.0 0.0 6.527883855890436E-5 0.0 12-13 0.0 0.0 0.0 6.527883855890436E-5 0.0 14-15 0.0 0.0 0.0 6.527883855890436E-5 0.0 16-17 6.527883855890436E-5 0.0 0.0 1.305576771178087E-4 0.0 18-19 6.527883855890436E-5 0.0 0.0 1.305576771178087E-4 0.0 20-21 6.527883855890436E-5 0.0 0.0 3.263941927945218E-4 0.0 22-23 6.527883855890436E-5 0.0 0.0 5.54870127750687E-4 0.0 24-25 6.527883855890436E-5 0.0 0.0 0.0011423796747808263 0.0 26-27 6.527883855890436E-5 0.0 0.0 0.0028396294773123396 1.305576771178087E-4 28-29 6.527883855890436E-5 0.0 0.0 0.006560523275169888 1.305576771178087E-4 30-31 6.527883855890436E-5 0.0 0.0 0.017625286410904176 1.305576771178087E-4 32-33 6.527883855890436E-5 0.0 0.0 0.03923258197390152 1.305576771178087E-4 34-35 6.527883855890436E-5 0.0 0.0 0.07086017925569069 1.305576771178087E-4 36-37 6.527883855890436E-5 3.263941927945218E-5 0.0 0.1220387886858717 1.305576771178087E-4 38-39 6.527883855890436E-5 6.527883855890436E-5 0.0 0.1974684866406857 1.305576771178087E-4 40-41 6.527883855890436E-5 6.527883855890436E-5 0.0 0.2777940974874175 1.305576771178087E-4 42-43 6.527883855890436E-5 6.527883855890436E-5 0.0 0.35514952117971915 1.305576771178087E-4 44-45 6.527883855890436E-5 6.527883855890436E-5 0.0 0.4506198225721168 1.305576771178087E-4 46-47 6.527883855890436E-5 6.527883855890436E-5 0.0 0.5665224004334515 1.305576771178087E-4 48-49 6.527883855890436E-5 6.527883855890436E-5 0.0 0.6882347949265286 1.305576771178087E-4 50-51 6.527883855890436E-5 9.791825783835653E-5 0.0 0.8117423574799757 1.9583651567671307E-4 52-53 6.527883855890436E-5 1.305576771178087E-4 0.0 0.9269921469557214 2.611153542356174E-4 54-55 6.527883855890436E-5 1.305576771178087E-4 0.0 1.049161493318711 2.611153542356174E-4 56-57 6.527883855890436E-5 1.305576771178087E-4 0.0 1.1935582842110073 2.611153542356174E-4 58-59 6.527883855890436E-5 1.305576771178087E-4 0.0 1.3510761216536435 2.611153542356174E-4 60-61 6.527883855890436E-5 1.305576771178087E-4 0.0 1.5211274960995893 2.611153542356174E-4 62-63 1.305576771178087E-4 1.305576771178087E-4 0.0 1.6870010248777654 2.611153542356174E-4 64-65 1.305576771178087E-4 1.305576771178087E-4 0.0 1.846346669800051 2.611153542356174E-4 66-67 1.305576771178087E-4 1.305576771178087E-4 0.0 2.026091951771994 2.611153542356174E-4 68-69 1.305576771178087E-4 1.305576771178087E-4 0.0 2.229304976205863 2.611153542356174E-4 70-71 1.305576771178087E-4 1.305576771178087E-4 0.0 2.451383584983256 2.611153542356174E-4 72-73 1.9583651567671307E-4 1.305576771178087E-4 0.0 2.6690558721579225 2.937547735150696E-4 74-75 1.9583651567671307E-4 1.305576771178087E-4 0.0 2.884476039402307 3.263941927945218E-4 76-77 1.9583651567671307E-4 1.305576771178087E-4 0.0 3.113735320421179 3.263941927945218E-4 78-79 1.9583651567671307E-4 1.305576771178087E-4 0.0 3.3682248725430677 3.263941927945218E-4 80-81 1.9583651567671307E-4 1.305576771178087E-4 0.0 3.633518072446455 3.263941927945218E-4 82-83 1.9583651567671307E-4 1.305576771178087E-4 0.0 3.908309343360163 3.263941927945218E-4 84-85 1.9583651567671307E-4 1.305576771178087E-4 0.0 4.185156897688476 3.263941927945218E-4 86-87 1.9583651567671307E-4 1.305576771178087E-4 0.0 4.473526167022436 3.263941927945218E-4 88-89 1.9583651567671307E-4 1.305576771178087E-4 0.0 4.779651280444418 3.59033612073974E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7365 0.0 34.314987 1 GTATCAA 10700 0.0 29.924097 1 TACATGG 8535 0.0 25.213877 2 GTACATG 8745 0.0 25.205954 1 ATCAACG 13050 0.0 24.350271 3 TATCAAC 13135 0.0 24.304358 2 TCAACGC 13145 0.0 24.281113 4 CAACGCA 13300 0.0 23.926714 5 ACATGGG 8825 0.0 23.574778 3 AACGCAG 13640 0.0 23.434763 6 GAGTACT 8525 0.0 22.75923 12-13 ACGCAGA 15180 0.0 21.026035 7 CGCAGAG 15230 0.0 20.957006 8 GTACTTT 9185 0.0 20.891138 14-15 TACCTGG 1145 0.0 20.74481 2 CATGGGA 5565 0.0 20.73963 4 AATACCG 115 0.003725511 20.65057 5 AGTACTT 8920 0.0 20.526712 12-13 GCAGAGT 16035 0.0 19.845667 9 CATGGGG 3110 0.0 19.54832 4 >>END_MODULE