FastQCFastQC Report
Thu 26 May 2016
SRR937925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530549
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129140.8437495303972626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101830.6653168242245102No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69100.4514719881558839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46440.3034205373366028No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42340.2766327637991335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23780.15536908651732156No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22400.14635271396080754No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20530.13413487578640082No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17420.11381536951773515No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16230.10604038158856723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.032.801191
GTATCAA111350.030.6287381
GTATACG650.00572621229.3534981
TATCAAC132750.025.5596242
TACATGG84250.025.2132262
ATCAACG134050.025.1344993
GTACATG85800.025.1283761
TCAACGC134550.025.0764144
CAACGCA136250.024.7635355
AACGCAG140400.024.065416
ACATGGG88000.023.7607863
GAGTACT80700.023.26709712-13
TAGCCCT8500.022.3630924
ACGCAGA152700.022.1399757
CGCAGAG154200.021.9862798
GTACTTT87950.021.39056814-15
AGTACTT83000.021.19055212-13
GCAGAGT160500.020.67829
CATGGGG31250.020.5293184
TACCTGG12950.019.4489842
Produced by FastQC (version 0.11.5)