##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1530549 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10865055610765 33.0 31.0 34.0 31.0 34.0 2 32.28422742427717 34.0 31.0 34.0 31.0 34.0 3 32.351600634804896 34.0 31.0 34.0 30.0 34.0 4 35.86772197427198 37.0 35.0 37.0 35.0 37.0 5 35.896196070821645 37.0 35.0 37.0 35.0 37.0 6 35.933058660650524 37.0 37.0 37.0 35.0 37.0 7 35.88663087558778 37.0 36.0 37.0 35.0 37.0 8 35.87685529832759 37.0 36.0 37.0 35.0 37.0 9 37.574736254768716 39.0 38.0 39.0 35.0 39.0 10-11 37.50539185612483 39.0 38.0 39.0 35.0 39.0 12-13 37.41674817336786 39.0 37.5 39.0 35.0 39.0 14-15 38.69459030713816 40.0 38.0 41.0 35.0 41.0 16-17 38.62741571815081 40.0 38.0 41.0 34.5 41.0 18-19 38.592336148662994 40.0 38.0 41.0 34.5 41.0 20-21 38.500241743322164 40.0 38.0 41.0 34.0 41.0 22-23 38.33997376104914 40.0 38.0 41.0 34.0 41.0 24-25 38.292186986499615 40.0 38.0 41.0 34.0 41.0 26-27 38.28917140189566 40.0 38.0 41.0 34.0 41.0 28-29 38.19077729625121 40.0 38.0 41.0 34.0 41.0 30-31 37.98764528283642 40.0 38.0 41.0 34.0 41.0 32-33 37.74381349437358 40.0 37.0 41.0 33.0 41.0 34-35 37.5373294157848 40.0 37.0 41.0 33.0 41.0 36-37 37.320200790696674 40.0 36.5 41.0 32.5 41.0 38-39 37.08784135627151 40.0 36.0 41.0 31.5 41.0 40-41 36.86294591025835 39.5 35.5 41.0 31.0 41.0 42-43 36.58197189374532 39.0 35.0 41.0 30.5 41.0 44-45 36.3317231267996 39.0 35.0 41.0 30.0 41.0 46-47 36.13892596708763 39.0 35.0 41.0 30.0 41.0 48-49 35.86511147307274 39.0 35.0 41.0 28.5 41.0 50-51 35.21520055875375 38.5 34.5 40.5 27.5 40.5 52-53 35.28866439427944 38.5 35.0 40.0 27.5 41.0 54-55 35.430360935847204 39.0 35.0 41.0 27.5 41.0 56-57 35.24047482308636 39.0 35.0 41.0 27.0 41.0 58-59 35.001434125924746 38.0 35.0 40.0 26.5 41.0 60-61 34.65139338890816 38.0 34.5 40.0 26.0 41.0 62-63 34.259041690269314 37.5 34.0 40.0 25.5 41.0 64-65 33.85439734369824 37.0 34.0 40.0 23.5 41.0 66-67 33.44707454645359 36.0 33.5 39.0 22.5 41.0 68-69 32.98199730946216 36.0 33.0 39.0 21.0 41.0 70-71 32.515565329826096 35.0 33.0 38.5 19.5 40.0 72-73 32.041554697040084 35.0 32.0 37.5 18.0 40.0 74-75 31.54036492787882 35.0 31.5 37.0 14.0 39.0 76-77 31.022907466536516 35.0 31.0 36.5 7.5 39.0 78-79 30.48560745196658 35.0 30.5 36.0 6.0 38.5 80-81 29.99579889307693 34.0 30.0 35.5 2.0 37.0 82-83 29.484671186613433 34.0 29.5 35.0 2.0 37.0 84-85 29.017645629117396 34.0 29.0 35.0 2.0 36.5 86-87 28.539782130464296 34.0 29.0 35.0 2.0 36.0 88-89 28.025586243890267 34.0 27.5 35.0 2.0 35.5 90-91 27.450317173772287 33.0 26.5 35.0 2.0 35.0 92-93 26.87641493346505 33.0 25.0 35.0 2.0 35.0 94-95 26.330640508732486 33.0 24.5 35.0 2.0 35.0 96-97 25.55008758295226 32.5 21.5 35.0 2.0 35.0 98-99 24.551877463576794 32.0 10.0 34.5 2.0 35.0 100-101 21.748067523483403 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3350812693021794 1101 2 0.15880283096552716 1101 3 0.15476805861813148 1101 4 0.08231186745850039 1101 5 0.09255008451700775 1101 6 0.09828175747712464 1101 7 0.2877889829996221 1101 8 0.11908631976546502 1101 9 0.2702011305416505 1101 10-11 0.17383950870130604 1101 12-13 0.07008578530636811 1101 14-15 0.13181061202161004 1101 16-17 0.34406279077811774 1101 18-19 0.2491209803920924 1101 20-21 0.31714422330249903 1101 22-23 0.3498327473752525 1101 24-25 0.4873169707583287 1101 26-27 0.26668030820994915 1101 28-29 0.320609240841911 1101 30-31 0.4774333007439182 1101 32-33 0.3795757120948906 1101 34-35 0.4671763637703492 1101 36-37 0.38488832253019467 1101 38-39 0.5133876986207611 1101 40-41 0.5487766511106358 1101 42-43 0.6303780115102242 1101 44-45 0.8337880808551148 1101 46-47 0.7771325560075795 1101 48-49 0.7453048564396028 1101 50-51 0.8286707117912471 1101 52-53 0.7565749044383594 1101 54-55 0.64293641585936 1101 56-57 0.5923787297459029 1101 58-59 0.5408638316150061 1101 60-61 0.7369467548027089 1101 62-63 0.7664005951549839 1101 64-65 0.7770105126618674 1101 66-67 0.5203078060481303 1101 68-69 0.7953370038046188 1101 70-71 0.8631436751888515 1101 72-73 0.8378061407066966 1101 74-75 0.7917102082081193 1101 76-77 0.7197702858238877 1101 78-79 0.6462083732839758 1101 80-81 0.6952868958926643 1101 82-83 0.5733035822452699 1101 84-85 0.5890839701830259 1101 86-87 0.4606088339419827 1101 88-89 0.3573549453552687 1101 90-91 0.3015009807008795 1101 92-93 0.1919124376182424 1101 94-95 0.30061534474434026 1101 96-97 0.44076138266431286 1101 98-99 0.5182093328981772 1101 100-101 0.5234740590400335 1102 1 0.21836412896283974 1102 2 0.2537169044118741 1102 3 0.13767736977597167 1102 4 0.20986321413074904 1102 5 0.07200309721037712 1102 6 0.14347826686523035 1102 7 -0.01657168236903317 1102 8 0.05988057174563721 1102 9 0.1656657786080089 1102 10-11 0.20996401447090562 1102 12-13 0.1121816081658693 1102 14-15 0.17498054929561135 1102 16-17 0.1338080135424775 1102 18-19 0.18672850231239835 1102 20-21 0.23287459250983034 1102 22-23 0.23186524635494976 1102 24-25 0.06877991875008149 1102 26-27 0.19735235757969605 1102 28-29 0.29778716898646707 1102 30-31 0.2941122283971609 1102 32-33 0.3973815240624816 1102 34-35 0.39282773409807703 1102 36-37 0.34938951700157617 1102 38-39 0.404118132815654 1102 40-41 0.5167562398173544 1102 42-43 0.628181431935559 1102 44-45 0.3900096000563309 1102 46-47 0.44976655386039255 1102 48-49 0.3745822727421242 1102 50-51 0.45602709577935485 1102 52-53 0.42766609758399454 1102 54-55 0.2923202038023547 1102 56-57 0.2525559118332481 1102 58-59 0.2515974372028964 1102 60-61 0.3071642551731415 1102 62-63 0.27363111123420225 1102 64-65 0.21713305320759702 1102 66-67 0.20268239955093037 1102 68-69 0.23415121476463696 1102 70-71 0.24651881718667568 1102 72-73 0.41273393952600657 1102 74-75 0.4263017873798596 1102 76-77 0.4209923119280923 1102 78-79 0.31828451790989476 1102 80-81 0.3202018271748166 1102 82-83 0.2836254266442495 1102 84-85 0.3682026868606556 1102 86-87 0.29730159094829034 1102 88-89 0.3977727893799212 1102 90-91 0.40017653419993593 1102 92-93 0.5935342672000594 1102 94-95 0.6836392939604643 1102 96-97 0.6348711700510066 1102 98-99 0.7259563701152523 1102 100-101 0.3955161501837914 1103 1 0.1918947944135283 1103 2 0.31271679537034913 1103 3 0.16007152515023648 1103 4 0.12635871864605974 1103 5 0.12336115983986673 1103 6 0.04759260186062164 1103 7 0.07794699842677488 1103 8 0.17734357197362982 1103 9 0.14035674501330675 1103 10-11 0.11008371232674818 1103 12-13 0.06713952805008461 1103 14-15 0.09561442750614191 1103 16-17 0.08930742372696443 1103 18-19 0.0997512820782589 1103 20-21 0.16040633846289865 1103 22-23 0.20678743035755787 1103 24-25 0.3062200698221602 1103 26-27 0.06618729844945648 1103 28-29 0.2520283886654866 1103 30-31 0.3408090136547557 1103 32-33 0.4466340939729747 1103 34-35 0.41892682310569995 1103 36-37 0.05991772406151341 1103 38-39 0.24628597746121272 1103 40-41 0.2668642900557643 1103 42-43 0.2699676312436381 1103 44-45 0.3448027185448268 1103 46-47 0.47089805793952877 1103 48-49 0.42906239248953426 1103 50-51 0.3726527066597214 1103 52-53 0.6009762017682121 1103 54-55 0.5713978611938799 1103 56-57 0.3788666405280239 1103 58-59 0.46948322116189445 1103 60-61 0.4213986349746861 1103 62-63 0.3976708023493032 1103 64-65 0.6443200792487005 1103 66-67 0.6932676551541803 1103 68-69 0.6546834860996 1103 70-71 0.5719029407406282 1103 72-73 0.6940199356203394 1103 74-75 0.7864347189116963 1103 76-77 0.7501698414436504 1103 78-79 0.6599693581640622 1103 80-81 0.7900305328519153 1103 82-83 0.7257553545777142 1103 84-85 0.684881331573628 1103 86-87 0.644618673790113 1103 88-89 0.5795816891509347 1103 90-91 0.610148133839111 1103 92-93 0.6490884459686797 1103 94-95 0.7407549969313472 1103 96-97 0.5525693530226832 1103 98-99 0.5201875777997067 1103 100-101 0.3393337435382051 1104 1 0.33224840114975507 1104 2 0.1923838456858249 1104 3 0.14279608154848233 1104 4 0.06813376297811402 1104 5 0.10360480807764105 1104 6 0.1165378550500904 1104 7 0.17235366116947404 1104 8 0.09714941068367011 1104 9 0.02031246919105456 1104 10-11 0.10693407429378254 1104 12-13 0.10505890181525501 1104 14-15 0.17883263495392754 1104 16-17 0.2890823893297778 1104 18-19 0.2348042556064911 1104 20-21 0.2896905210097529 1104 22-23 0.2507956669351685 1104 24-25 0.3191508780714827 1104 26-27 0.3589119673180363 1104 28-29 0.27958549547856393 1104 30-31 0.2596743191804194 1104 32-33 0.31610383382908935 1104 34-35 0.38701523978579644 1104 36-37 0.4248638132804885 1104 38-39 0.3663808649346407 1104 40-41 0.2457226786557669 1104 42-43 0.35932812491343924 1104 44-45 0.36876218048673337 1104 46-47 0.4310962119264232 1104 48-49 0.44639032224807096 1104 50-51 0.5903067066672136 1104 52-53 0.5106934513436343 1104 54-55 0.615512944590634 1104 56-57 0.43619370801398105 1104 58-59 0.5557610903738919 1104 60-61 0.5069707528286003 1104 62-63 0.6376262640962622 1104 64-65 0.6096717177540469 1104 66-67 0.7577862633712655 1104 68-69 0.8945869303161942 1104 70-71 0.8246758031481747 1104 72-73 0.7151709195483207 1104 74-75 0.564054621662347 1104 76-77 0.5730374975993406 1104 78-79 0.6599988816272493 1104 80-81 0.670820412021282 1104 82-83 0.6966260061933518 1104 84-85 0.62283285310982 1104 86-87 0.6106245147495599 1104 88-89 0.6670339361207951 1104 90-91 0.42453412078505437 1104 92-93 0.5028348328591434 1104 94-95 0.5368926718695839 1104 96-97 0.5514469927951957 1104 98-99 0.8308775083305271 1104 100-101 0.7278564269304582 1105 1 0.24347653690610827 1105 2 0.28759609271913433 1105 3 0.3054253542848073 1105 4 0.2625710504712515 1105 5 0.2065203608072892 1105 6 0.19681424826104887 1105 7 0.1900206076122899 1105 8 0.21455713477606508 1105 9 0.33736507263763116 1105 10-11 0.26519159003883175 1105 12-13 0.13153355645474818 1105 14-15 0.26063112975245417 1105 16-17 0.2100197908003949 1105 18-19 0.3189571932996671 1105 20-21 0.42308915485207166 1105 22-23 0.28785952838745743 1105 24-25 0.356707986903821 1105 26-27 0.21484180959241428 1105 28-29 0.2589036044480295 1105 30-31 0.13614390815403254 1105 32-33 0.19320597599437406 1105 34-35 0.39022254866826955 1105 36-37 0.35625177079526793 1105 38-39 0.24645995919358654 1105 40-41 0.3025070463602262 1105 42-43 0.38499576657522283 1105 44-45 0.35863652577126004 1105 46-47 0.2860144494589818 1105 48-49 0.36545015212903564 1105 50-51 0.15419785424141708 1105 52-53 0.2597456041206314 1105 54-55 0.36793616700044396 1105 56-57 0.3825297468204738 1105 58-59 0.2549723312611505 1105 60-61 0.18281093281694893 1105 62-63 0.20416334870074593 1105 64-65 0.2783086082037798 1105 66-67 0.40448554535679904 1105 68-69 0.2911161194690237 1105 70-71 0.30370241798201647 1105 72-73 0.3225752636188375 1105 74-75 0.3149166582521765 1105 76-77 0.28955388284497374 1105 78-79 0.2594451082738729 1105 80-81 0.21877348998100743 1105 82-83 0.21585850540948215 1105 84-85 0.3564397469570437 1105 86-87 0.4385303073384925 1105 88-89 0.37760471439048615 1105 90-91 0.42576975023334285 1105 92-93 0.48335254391181337 1105 94-95 0.5166649439866724 1105 96-97 0.4867014507995151 1105 98-99 0.2896807622348021 1105 100-101 0.5375646882454568 1106 1 0.10194272535282778 1106 2 0.07544340976826902 1106 3 -0.01814230348142587 1106 4 0.06378948407125051 1106 5 0.10006338932760883 1106 6 0.12614359614977388 1106 7 0.12945304087989484 1106 8 0.11011209457502247 1106 9 0.11341306834817999 1106 10-11 -0.30277706926661807 1106 12-13 -0.07969333744573248 1106 14-15 -0.02452526053048132 1106 16-17 0.11077641559985096 1106 18-19 0.12837765018930725 1106 20-21 -0.005066984043843092 1106 22-23 -0.009431922700230189 1106 24-25 0.06630232677071035 1106 26-27 0.11292713418649925 1106 28-29 0.01335507547964454 1106 30-31 0.2504254048171006 1106 32-33 0.14233267755456325 1106 34-35 -0.07525119892747512 1106 36-37 -0.014708185174420407 1106 38-39 0.1336527513182446 1106 40-41 0.2935677742801346 1106 42-43 0.09559419294262739 1106 44-45 0.13309414367468975 1106 46-47 0.29843764488023083 1106 48-49 0.5043573452218197 1106 50-51 0.3923077078606809 1106 52-53 0.25536616674313706 1106 54-55 0.029949877197147146 1106 56-57 0.041185644769036855 1106 58-59 0.23427925476016043 1106 60-61 -0.10589995290886378 1106 62-63 -0.03694039061844023 1106 64-65 0.011662152718905361 1106 66-67 -0.1522895016843151 1106 68-69 -0.26243891883617465 1106 70-71 0.02962772680803738 1106 72-73 0.006655491988482254 1106 74-75 0.15193143329420522 1106 76-77 0.22483133142547373 1106 78-79 0.29685709150026085 1106 80-81 0.25061925527908 1106 82-83 0.20817783874114681 1106 84-85 0.13867699950578327 1106 86-87 0.05657738936999834 1106 88-89 0.2028452232779827 1106 90-91 -0.01590136372849571 1106 92-93 0.15326761518963394 1106 94-95 0.21953426366325957 1106 96-97 0.15988204773092463 1106 98-99 0.18220634148977766 1106 100-101 -0.253579055850917 1107 1 0.30607867948878464 1107 2 0.22283430715916808 1107 3 0.11927433393521625 1107 4 0.16321033349210268 1107 5 0.21782400708822536 1107 6 0.1586111286173093 1107 7 0.11046977189662499 1107 8 0.14038587484880338 1107 9 0.09814455307966341 1107 10-11 0.12744698595744097 1107 12-13 0.0965234637710708 1107 14-15 0.14389640789119085 1107 16-17 0.10460465942809094 1107 18-19 0.2480833948950476 1107 20-21 0.26050865653179045 1107 22-23 0.29994563667732876 1107 24-25 0.2781895386579265 1107 26-27 0.2596745309338999 1107 28-29 0.35275778988235373 1107 30-31 0.2969183263100277 1107 32-33 0.32616911139099614 1107 34-35 0.4534138297375563 1107 36-37 0.46034153987530146 1107 38-39 0.4738598415253321 1107 40-41 0.5039778596902238 1107 42-43 0.43708828443671877 1107 44-45 0.3680450036255465 1107 46-47 0.25369972514231875 1107 48-49 0.1369477278121991 1107 50-51 0.24706668761965744 1107 52-53 0.36853452991149993 1107 54-55 0.3631369603842316 1107 56-57 0.29405214763554 1107 58-59 0.17237156785247265 1107 60-61 0.3270589800500687 1107 62-63 0.33848373480568483 1107 64-65 0.42388281493956725 1107 66-67 0.2529842785894729 1107 68-69 0.3212600398627856 1107 70-71 0.33593403311434145 1107 72-73 0.32438393471692706 1107 74-75 0.17149970286247296 1107 76-77 0.3277621843563274 1107 78-79 0.16485170949487937 1107 80-81 0.15448903414885606 1107 82-83 0.1915053220686289 1107 84-85 0.28017401600280323 1107 86-87 0.4247913825839902 1107 88-89 0.27482954526229975 1107 90-91 0.40712718430005523 1107 92-93 0.4393475262695432 1107 94-95 0.39336718749618527 1107 96-97 0.538070300919177 1107 98-99 0.4367547210381595 1107 100-101 0.41797236570050345 1108 1 0.2579572658145892 1108 2 0.11928224280135424 1108 3 0.06324746497892875 1108 4 0.19211540971135577 1108 5 0.12618364717803132 1108 6 0.1340971194062348 1108 7 0.09809152856897185 1108 8 0.05151213653721243 1108 9 0.21901188568966035 1108 10-11 0.16167946811308553 1108 12-13 0.011330049642616302 1108 14-15 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-1.3273587782372758 2316 98-99 -1.320463144860458 2316 100-101 -1.2280727094449553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3726.0 3 863.0 4 892.0 5 974.0 6 1105.0 7 1386.0 8 1636.0 9 2005.0 10 2647.0 11 3330.0 12 4720.0 13 6203.0 14 7245.0 15 9012.0 16 11745.0 17 16747.0 18 14584.0 19 10343.0 20 9848.0 21 10104.0 22 10726.0 23 11541.0 24 13052.0 25 14579.0 26 16738.0 27 19409.0 28 23221.0 29 27612.0 30 33446.0 31 41704.0 32 52809.0 33 70227.0 34 98677.0 35 158817.0 36 221921.0 37 307990.0 38 254155.0 39 34807.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.36740773612973 18.44286132552219 13.89147465952075 22.29825627882733 2 15.596286495730071 20.287586091485768 40.68067837737487 23.43544903540929 3 19.552843269604782 25.518136323966356 29.374470880879144 25.554549525549714 4 12.481963472558654 16.446567093920702 36.9026534208109 34.16881601270975 5 13.234043081864435 37.97822693862178 33.80539468169207 14.982335297821713 6 31.552144875837435 35.81266028439627 17.6072307107683 15.027964128997995 7 28.205388052919428 33.68307840169468 20.41634657190772 17.695186973478176 8 26.570239046975946 34.59399143538659 19.6272281754994 19.20854134213807 9 27.14435594311656 16.615690337594252 20.219179694011288 36.020774025277895 10-11 25.07050118117676 26.87038178849711 26.651113797700678 21.408003232625454 12-13 26.294701371683615 24.531369773775545 27.404043628067843 21.769885226472997 14-15 23.018824877615252 25.58611810320229 26.565784437451807 24.829272581730656 16-17 21.506746415956307 28.854746941574643 27.299465817483608 22.33904082498544 18-19 21.428251555691247 26.96120717129781 30.32722715462053 21.283314118390418 20-21 22.41384721509345 26.012207361686407 30.607719995684597 20.966225427535544 22-23 21.983421419437175 26.10521691148589 30.429586470392874 21.48177519868407 24-25 21.55350808443742 26.384160119957873 30.693789283113727 21.36854251249098 26-27 21.41752226830761 26.532204706288475 30.54151346036163 21.508759565042286 28-29 21.303067084359125 26.884347941781527 30.562410490387638 21.250174483471714 30-31 21.38432403151814 26.720524155940446 30.44485715135683 21.45029466118459 32-33 21.044455356016183 26.7879921567448 30.395425334446575 21.772127152792443 34-35 21.670764000130767 26.795808950930073 30.261956912615645 21.271470136323515 36-37 21.777470488818278 26.521227455941627 30.199312849582054 21.501989205658038 38-39 21.495712864543776 26.69488589644205 30.24554782388168 21.5638534151325 40-41 21.64004838321177 26.681850968334224 30.311735739551644 21.366364908902362 42-43 21.40575246936155 26.809800251585152 30.012853785493753 21.771593493559543 44-45 21.510730107363155 27.030924170074112 29.892917848136257 21.565427874426472 46-47 21.7073031257097 27.184312469671745 29.358503266873946 21.749881137744605 48-49 21.70187903883556 27.196590647677603 29.304300771692077 21.79722954179476 50-51 21.83031237368646 27.52948119180183 29.11959811014524 21.520608324366467 52-53 21.881878401622846 27.386625642419837 29.05946598769273 21.672029968264592 54-55 21.553910344992094 27.515585732393 29.14351421463887 21.78698970797603 56-57 21.739744178333417 27.64684258422689 28.83203962964839 21.781373607791306 58-59 21.74009222251557 27.303537026242115 28.951151866538755 22.005218884703556 60-61 21.559605896198594 27.433257009570262 29.18562098209632 21.821516112134827 62-63 21.711067589123687 27.270511370282584 29.345614898373544 21.672806142220182 64-65 21.485701402412722 27.3460678856635 29.348002248042764 21.820228463881012 66-67 21.485506820204588 27.57017224006179 29.00299025162288 21.941330688110742 68-69 21.686610386635774 27.438720561053337 29.06621698405733 21.80845206825356 70-71 21.56970787354096 27.427662873941312 28.809450275426045 22.193178977091677 72-73 21.58903626092967 27.444054635683134 28.75353973407671 22.213369369310485 74-75 21.6154991925053 27.734550904191458 28.570966398939635 22.07898350436361 76-77 21.692922699694417 27.474140432417453 28.775418445315214 22.057518422572915 78-79 21.69772421783261 27.501043891811637 28.72025725153578 22.080974638819967 80-81 21.732912543418873 27.683393381609385 28.556002055810755 22.027692019160995 82-83 21.703949659953743 27.72623515503848 28.43772811068641 22.132087074321362 84-85 21.86302506730794 27.5284588441331 28.38862822490002 22.21988786365894 86-87 21.82367174222282 27.596809214803642 28.409991590472337 22.169527452501196 88-89 21.925124077466958 27.498200852217575 28.338097289284008 22.23857778103146 90-91 21.877421798878295 27.554126541003598 28.411371844164197 22.15707981595391 92-93 21.89475576179933 27.489056461957013 28.3792779470756 22.236909829168063 94-95 21.851508167668538 27.330319493005423 28.443039104202366 22.37513323512367 96-97 21.965220437895134 27.04799250129423 28.320360502632912 22.666426558177722 98-99 21.952523496424885 27.080234245303984 28.19986013254729 22.76738212572385 100-101 22.424229137162406 26.65298018099145 28.318263801448662 22.60452688039748 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4751.0 1 3007.0 2 2916.5 3 3719.0 4 2119.0 5 1622.0 6 2784.0 7 5213.5 8 7575.5 9 7862.5 10 5574.0 11 3404.0 12 3047.0 13 3080.5 14 2990.5 15 2958.0 16 2931.5 17 2932.0 18 3058.0 19 3239.0 20 3480.0 21 3971.0 22 4607.5 23 5558.0 24 6812.5 25 8095.0 26 9848.5 27 12099.0 28 13988.0 29 15840.5 30 18057.5 31 20862.5 32 24104.0 33 26194.5 34 27726.0 35 29958.5 36 33049.5 37 35917.5 38 37602.0 39 39075.0 40 40808.5 41 42459.5 42 43350.0 43 44705.5 44 46881.0 45 49441.0 46 51454.5 47 53276.0 48 58864.5 49 59825.0 50 55678.0 51 53451.0 52 51400.5 53 51092.0 54 51063.5 55 47756.5 56 43406.5 57 39662.0 58 36310.5 59 32481.5 60 27192.0 61 22862.0 62 19118.0 63 14650.5 64 10492.0 65 7846.5 66 6003.5 67 4741.5 68 3718.5 69 2696.0 70 2095.0 71 1599.0 72 1228.0 73 1050.0 74 835.5 75 597.0 76 414.5 77 275.0 78 188.5 79 112.5 80 61.0 81 35.5 82 25.5 83 19.0 84 14.5 85 12.5 86 8.5 87 6.5 88 3.5 89 2.5 90 3.0 91 4.0 92 5.0 93 2.0 94 1.5 95 3.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.474012919547169 2 0.051027441787227984 3 0.0577570531881044 4 0.0662507374804727 5 0.08023264854637127 6 0.10623638968762189 7 0.0688641788012014 8 0.050047401291954716 9 0.049263368895736105 10-11 0.07461374970680455 12-13 0.10617105365460368 14-15 0.05517627988388481 16-17 0.06023982244279667 18-19 0.06033782649232399 20-21 0.07474442177284099 22-23 0.06435599252294438 24-25 0.08790963242601184 26-27 0.06670808971160022 28-29 0.06759012615734616 30-31 0.07059558367618417 32-33 0.0706935877257115 34-35 0.07180430028702119 36-37 0.06821081847101922 38-39 0.040704348570349595 40-41 0.04439583443587889 42-43 0.04243575344533236 44-45 0.021038202631866083 46-47 0.02721245775208765 48-49 0.026461093372378147 50-51 0.02472968849739538 52-53 0.025938405108232407 54-55 0.02505636866248647 56-57 0.024925696596450032 58-59 0.022018243127139348 60-61 0.029041866676597743 62-63 0.018816777509246683 64-65 0.021495554862993607 66-67 0.01587665602342689 68-69 0.01858810139368292 70-71 0.04223974534627771 72-73 0.03897294369536682 74-75 0.033190704773254566 76-77 0.02358630791957657 78-79 0.014243255197971446 80-81 0.016497348337099958 82-83 0.020646186433756777 84-85 0.017575392881900548 86-87 0.009277716688586905 88-89 0.042991109725987214 90-91 0.05380422319050223 92-93 0.03505278171427377 94-95 0.05413090335559332 96-97 0.044755182617479086 98-99 0.034562761466637136 100-101 0.1647121392389267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1530549.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.049441715730254 #Duplication Level Percentage of deduplicated Percentage of total 1 79.33185545192133 48.431654856128084 2 12.612442533609203 15.399651506971445 3 3.7417504440142046 6.852953269399588 4 1.5009162390640172 3.6652039382772705 5 0.7575696270279292 2.3124601395424533 6 0.4604147134699987 1.6864836729030799 7 0.3102791034289318 1.325965622827553 8 0.20814201976470395 1.0165563283375734 9 0.15929522342001576 0.8752396011997032 >10 0.8387516255892973 9.19235176487581 >50 0.04999360905089834 2.090604885730419 >100 0.025484329122917888 2.9978664517936475 >500 0.0016060761292528442 0.6604651438313546 >1k 0.001177789161452086 1.5310842304000216 >5k 1.0707174195018962E-4 0.451683901808656 >10k+ 2.1414348390037924E-4 1.5097746859731587 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12914 0.8437495303972626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10183 0.6653168242245102 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6910 0.4514719881558839 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4644 0.3034205373366028 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4234 0.2766327637991335 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2378 0.15536908651732156 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2240 0.14635271396080754 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2053 0.13413487578640082 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1742 0.11381536951773515 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1623 0.10604038158856723 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.306720660364353E-4 0.0 0.0 0.0 0.0 7 1.306720660364353E-4 0.0 0.0 0.0 0.0 8 1.306720660364353E-4 0.0 0.0 0.0 0.0 9 1.306720660364353E-4 0.0 0.0 0.0 0.0 10-11 1.306720660364353E-4 0.0 0.0 0.0 0.0 12-13 1.306720660364353E-4 0.0 0.0 3.2668016509108824E-5 0.0 14-15 1.306720660364353E-4 3.2668016509108824E-5 0.0 6.533603301821765E-5 3.2668016509108824E-5 16-17 1.306720660364353E-4 6.533603301821765E-5 0.0 1.306720660364353E-4 6.533603301821765E-5 18-19 1.306720660364353E-4 6.533603301821765E-5 0.0 1.9600809905465295E-4 6.533603301821765E-5 20-21 1.306720660364353E-4 6.533603301821765E-5 0.0 1.9600809905465295E-4 1.306720660364353E-4 22-23 1.306720660364353E-4 6.533603301821765E-5 0.0 5.5535628065485E-4 1.306720660364353E-4 24-25 1.306720660364353E-4 6.533603301821765E-5 0.0 0.0011433805778188087 1.306720660364353E-4 26-27 1.306720660364353E-4 6.533603301821765E-5 0.0 0.0020907530565829648 1.306720660364353E-4 28-29 1.306720660364353E-4 6.533603301821765E-5 0.0 0.006043583054185132 1.306720660364353E-4 30-31 1.306720660364353E-4 6.533603301821765E-5 0.0 0.016236004205027084 1.306720660364353E-4 32-33 1.306720660364353E-4 6.533603301821765E-5 0.0 0.035804146093983275 1.306720660364353E-4 34-35 1.306720660364353E-4 6.533603301821765E-5 0.0 0.06566271318330874 1.6334008254554412E-4 36-37 1.306720660364353E-4 9.800404952732647E-5 0.0 0.11858489992806504 1.9600809905465295E-4 38-39 1.306720660364353E-4 1.306720660364353E-4 0.0 0.1942766941796702 1.9600809905465295E-4 40-41 1.306720660364353E-4 1.6334008254554412E-4 0.0 0.2752607071057509 1.9600809905465295E-4 42-43 1.306720660364353E-4 1.9600809905465295E-4 0.0 0.3546113192063763 2.2867611556376177E-4 44-45 1.306720660364353E-4 1.9600809905465295E-4 0.0 0.44846653063704595 2.613441320728706E-4 46-47 1.306720660364353E-4 1.9600809905465295E-4 0.0 0.5666920823835108 2.613441320728706E-4 48-49 1.306720660364353E-4 1.9600809905465295E-4 0.0 0.6874657394176861 2.613441320728706E-4 50-51 1.306720660364353E-4 1.9600809905465295E-4 0.0 0.8113101900037176 3.2668016509108824E-4 52-53 1.306720660364353E-4 1.9600809905465295E-4 0.0 0.9260402639837078 3.920161981093059E-4 54-55 1.306720660364353E-4 1.9600809905465295E-4 0.0 1.050995427131049 3.920161981093059E-4 56-57 1.306720660364353E-4 1.9600809905465295E-4 0.0 1.195616736216874 3.920161981093059E-4 58-59 1.306720660364353E-4 1.9600809905465295E-4 0.0 1.3525538875266325 3.920161981093059E-4 60-61 1.306720660364353E-4 3.2668016509108824E-4 0.0 1.5255963709753821 3.920161981093059E-4 62-63 1.306720660364353E-4 3.2668016509108824E-4 0.0 1.690994538561 3.920161981093059E-4 64-65 1.306720660364353E-4 3.2668016509108824E-4 0.0 1.8568827263942547 3.920161981093059E-4 66-67 1.306720660364353E-4 3.2668016509108824E-4 0.0 2.045932537932467 3.920161981093059E-4 68-69 1.306720660364353E-4 3.2668016509108824E-4 0.0 2.254811835491709 3.920161981093059E-4 70-71 1.306720660364353E-4 3.2668016509108824E-4 0.0 2.4772810279187403 3.920161981093059E-4 72-73 1.306720660364353E-4 3.2668016509108824E-4 0.0 2.6950786939849687 3.920161981093059E-4 74-75 1.306720660364353E-4 3.2668016509108824E-4 0.0 2.9096748944333046 3.920161981093059E-4 76-77 1.306720660364353E-4 3.2668016509108824E-4 0.0 3.1366522731385924 3.920161981093059E-4 78-79 1.306720660364353E-4 3.2668016509108824E-4 0.0 3.391397465876623 3.920161981093059E-4 80-81 1.306720660364353E-4 3.2668016509108824E-4 0.0 3.660059233647534 3.920161981093059E-4 82-83 1.306720660364353E-4 3.2668016509108824E-4 0.0 3.9377700419914685 3.920161981093059E-4 84-85 1.306720660364353E-4 3.2668016509108824E-4 0.0 4.214729485955694 3.920161981093059E-4 86-87 1.306720660364353E-4 3.2668016509108824E-4 0.0 4.500705302476431 3.920161981093059E-4 88-89 1.306720660364353E-4 3.2668016509108824E-4 0.0 4.798506940973468 3.920161981093059E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7620 0.0 32.80119 1 GTATCAA 11135 0.0 30.628738 1 GTATACG 65 0.005726212 29.353498 1 TATCAAC 13275 0.0 25.559624 2 TACATGG 8425 0.0 25.213226 2 ATCAACG 13405 0.0 25.134499 3 GTACATG 8580 0.0 25.128376 1 TCAACGC 13455 0.0 25.076414 4 CAACGCA 13625 0.0 24.763535 5 AACGCAG 14040 0.0 24.06541 6 ACATGGG 8800 0.0 23.760786 3 GAGTACT 8070 0.0 23.267097 12-13 TAGCCCT 850 0.0 22.363092 4 ACGCAGA 15270 0.0 22.139975 7 CGCAGAG 15420 0.0 21.986279 8 GTACTTT 8795 0.0 21.390568 14-15 AGTACTT 8300 0.0 21.190552 12-13 GCAGAGT 16050 0.0 20.6782 9 CATGGGG 3125 0.0 20.529318 4 TACCTGG 1295 0.0 19.448984 2 >>END_MODULE