FastQCFastQC Report
Thu 26 May 2016
SRR937925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530549
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122490.8003010684401479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.6502895366303202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70310.4593776481510882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46630.30466192196394887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.27225524958691294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25050.16366676271063518No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.16124932948896115No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19920.13014937777228955No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16460.10754311034798625No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15510.10133618721125558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76550.033.697781
GTATCAA109650.028.6377871
GTACATG86250.025.5567441
TACATGG84850.025.4185432
ACATGGG86150.023.763583
TATCAAC133450.023.566012
ATCAACG133500.023.4473653
TCAACGC133900.023.3757934
CAACGCA134850.023.1758925
AACGCAG138350.022.6582456
GAGTACT84900.022.60212912-13
CATGGGG31200.020.7035984
ACGCAGA152350.020.4825677
TACCTGG11600.020.4766372
ATGGGAT20200.020.4564065
GTACTTT94100.020.36711914-15
CATGGGA55500.020.1967074
CGCAGAG154500.020.1667948
AGTACTT88950.019.9443712-13
GTGTAGC11350.019.2534521
Produced by FastQC (version 0.11.5)