##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937925_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1530549 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40737343267024 33.0 31.0 34.0 31.0 34.0 2 32.62305813142866 34.0 31.0 34.0 31.0 34.0 3 32.679541131972904 34.0 31.0 34.0 31.0 34.0 4 36.1725348224722 37.0 35.0 37.0 35.0 37.0 5 36.13466148421253 37.0 35.0 37.0 35.0 37.0 6 36.18294742605431 37.0 37.0 37.0 35.0 37.0 7 36.146122077764254 37.0 36.0 37.0 35.0 37.0 8 36.147172681175185 37.0 36.0 37.0 35.0 37.0 9 37.82691439476946 39.0 38.0 39.0 35.0 39.0 10-11 37.81117102425339 39.0 38.0 39.0 35.0 39.0 12-13 37.7526933146211 39.0 38.0 39.0 35.0 39.0 14-15 39.00993597722125 40.0 38.0 41.0 35.5 41.0 16-17 38.95648620200987 40.0 38.0 41.0 35.5 41.0 18-19 39.00143967948756 40.0 38.5 41.0 35.5 41.0 20-21 38.93175128662983 40.0 38.0 41.0 35.0 41.0 22-23 38.76417644910421 40.0 38.0 41.0 35.0 41.0 24-25 38.70670458770023 40.0 38.0 41.0 34.5 41.0 26-27 38.648188329808455 40.0 38.0 41.0 34.5 41.0 28-29 38.57522888845767 40.0 38.0 41.0 34.5 41.0 30-31 38.34230201058574 40.0 38.0 41.0 34.0 41.0 32-33 38.1287025113211 40.0 37.0 41.0 34.0 41.0 34-35 37.901671883748904 40.0 37.0 41.0 33.0 41.0 36-37 37.749603900299824 40.0 37.0 41.0 33.0 41.0 38-39 37.71624038171924 40.0 37.0 41.0 33.0 41.0 40-41 37.63540141478646 40.0 37.0 41.0 33.0 41.0 42-43 37.45913786490991 40.0 37.0 41.0 33.0 41.0 44-45 37.213175468410356 40.0 36.0 41.0 32.5 41.0 46-47 36.83294785073853 40.0 36.0 41.0 31.0 41.0 48-49 36.45108356543959 39.5 35.0 41.0 30.5 41.0 50-51 36.13808639906334 39.0 35.0 41.0 30.0 41.0 52-53 35.70052314561637 39.0 35.0 40.5 28.0 41.0 54-55 35.48002906146749 39.0 35.0 40.0 28.0 41.0 56-57 35.23011089484884 38.0 35.0 40.0 27.5 41.0 58-59 34.899923491505334 38.0 34.5 40.0 26.5 41.0 60-61 34.533013970803935 37.5 34.0 40.0 26.0 41.0 62-63 34.16421754546898 37.0 34.0 40.0 26.0 41.0 64-65 33.77010471406012 36.5 33.0 39.5 25.0 41.0 66-67 33.34837140137297 36.0 33.0 39.0 23.5 40.5 68-69 32.88176203440726 35.0 32.5 39.0 22.0 40.0 70-71 32.36322097495736 35.0 32.0 38.0 21.0 40.0 72-73 31.935938019625638 35.0 31.0 37.0 20.0 39.5 74-75 31.489570735729465 35.0 31.0 37.0 18.5 39.0 76-77 30.200941949588024 33.5 29.5 35.5 18.0 37.5 78-79 30.95259021436099 34.0 31.0 36.0 18.0 38.0 80-81 30.789859390323343 34.5 31.0 35.5 17.5 37.0 82-83 30.41176303404857 34.0 31.0 35.0 12.0 37.0 84-85 30.01268760425181 34.0 30.0 35.0 7.0 36.5 86-87 29.54851167783586 34.0 30.0 35.0 4.5 36.0 88-89 29.040433204033324 34.0 29.0 35.0 2.0 35.5 90-91 28.65619624069533 34.0 29.0 35.0 2.0 35.0 92-93 28.215747421350116 34.0 29.0 35.0 2.0 35.0 94-95 27.7541091464566 33.0 27.5 35.0 2.0 35.0 96-97 27.112724584446497 33.0 26.5 35.0 2.0 35.0 98-99 26.34746976411732 33.0 25.0 35.0 2.0 35.0 100-101 23.880848309985502 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08382004730446369 1101 2 0.11251728393417437 1101 3 0.1015339921306051 1101 4 0.010524282835206122 1101 5 0.0031175334546986733 1101 6 0.008368284883367494 1101 7 0.14469400059478943 1101 8 0.06692154654737692 1101 9 0.09861398098040297 1101 10-11 0.04349557392253445 1101 12-13 0.13641187650933517 1101 14-15 0.10052994261967996 1101 16-17 0.10318836827151756 1101 18-19 0.15081662016265796 1101 20-21 0.1521836857916341 1101 22-23 0.1645777148070593 1101 24-25 0.0959735993131332 1101 26-27 0.16585408051070516 1101 28-29 0.31396182398834327 1101 30-31 0.30268873590836165 1101 32-33 0.186189764098863 1101 34-35 0.0819903016015644 1101 36-37 0.17462569269601858 1101 38-39 0.37381270549854406 1101 40-41 0.4819615375316886 1101 42-43 0.42105125201299387 1101 44-45 0.30508027083320854 1101 46-47 0.5055046420430855 1101 48-49 0.614871766247461 1101 50-51 0.5063428739254192 1101 52-53 0.5678578960302474 1101 54-55 0.5702192493795266 1101 56-57 0.6081500955723556 1101 58-59 0.5126992786475384 1101 60-61 0.48227295964766626 1101 62-63 0.3483083993295324 1101 64-65 0.5212451466700472 1101 66-67 0.3775172143180825 1101 68-69 0.455563952545333 1101 70-71 0.4818584745889751 1101 72-73 0.2785124110171999 1101 74-75 0.4313266967262521 1101 76-77 0.24452023609918072 1101 78-79 0.4526162727794194 1101 80-81 0.3416933606369703 1101 82-83 0.4648034778178918 1101 84-85 0.5530459937117982 1101 86-87 0.4289000762386941 1101 88-89 0.42892681278867073 1101 90-91 0.5274756201937514 1101 92-93 0.552033386171022 1101 94-95 0.6339847635104334 1101 96-97 0.7782007580336732 1101 98-99 0.9019276922286856 1101 100-101 1.2027021894068461 1102 1 0.2138913516568124 1102 2 0.17861119355987398 1102 3 0.1423804941101281 1102 4 0.07819972975466527 1102 5 0.13973750439225796 1102 6 0.08791877891881228 1102 7 0.10920611297648009 1102 8 0.12663731694892277 1102 9 0.12213791849875832 1102 10-11 0.160462526447823 1102 12-13 0.22741031940736178 1102 14-15 0.292365945208779 1102 16-17 0.2557089758272397 1102 18-19 0.26789223189057054 1102 20-21 0.18745425500468826 1102 22-23 0.13296022639434568 1102 24-25 0.2710404715137926 1102 26-27 0.1381774378201115 1102 28-29 0.17027224617353198 1102 30-31 0.19867432426674725 1102 32-33 0.11224015805345999 1102 34-35 0.27079918358155197 1102 36-37 0.2568778496331774 1102 38-39 0.2163883965112774 1102 40-41 0.2824232091677459 1102 42-43 0.3664138696079817 1102 44-45 0.503704354183661 1102 46-47 0.6160003931563196 1102 48-49 0.5422848193890886 1102 50-51 0.5575538521441885 1102 52-53 0.6447875716903226 1102 54-55 0.7823576260942318 1102 56-57 0.7587000203167662 1102 58-59 0.685736199380905 1102 60-61 0.6771605181689679 1102 62-63 0.8080579632629892 1102 64-65 0.8461993304392763 1102 66-67 0.7393965506131153 1102 68-69 0.9869997357364326 1102 70-71 0.9274542675542037 1102 72-73 0.8638041661752602 1102 74-75 0.9296869305774962 1102 76-77 0.7823076435893874 1102 78-79 0.7935683145586232 1102 80-81 0.7202412265754283 1102 82-83 0.8289053887121476 1102 84-85 0.9055668612177605 1102 86-87 0.7678117685407884 1102 88-89 0.6587074927665206 1102 90-91 0.8161475083943479 1102 92-93 0.9695418150577346 1102 94-95 0.9207876094457212 1102 96-97 0.9327788717827019 1102 98-99 0.846613919284831 1102 100-101 0.9302753718997216 1103 1 0.05459655293755361 1103 2 0.1602144013632838 1103 3 0.11528912722504714 1103 4 0.0795553141676777 1103 5 0.03974939983135073 1103 6 0.08465050702872645 1103 7 -0.08316990185316797 1103 8 -0.0027677128392369355 1103 9 -0.048449121420347296 1103 10-11 0.0010200260265236238 1103 12-13 -0.031248321142207658 1103 14-15 0.12676953763138954 1103 16-17 0.16513759823625662 1103 18-19 0.06433822164066783 1103 20-21 0.15120092180352174 1103 22-23 0.15647627036781842 1103 24-25 0.10331304558767584 1103 26-27 0.011918751671352368 1103 28-29 -0.04829479949503934 1103 30-31 -0.03918291063843071 1103 32-33 0.009904439023721068 1103 34-35 -0.006043122547566782 1103 36-37 0.057872110083813766 1103 38-39 0.17688959101162283 1103 40-41 0.25566664932873095 1103 42-43 0.25358587765722973 1103 44-45 0.3192395498140641 1103 46-47 0.29647526899767485 1103 48-49 0.2123266516832274 1103 50-51 0.43891493819777594 1103 52-53 0.4206081091659115 1103 54-55 0.38320567177271414 1103 56-57 0.42245907819798845 1103 58-59 0.5342450565265935 1103 60-61 0.5385875743451791 1103 62-63 0.587892220145001 1103 64-65 0.48756869373590206 1103 66-67 0.3448359879588452 1103 68-69 0.5002135859926753 1103 70-71 0.4727506126805352 1103 72-73 0.10254017664324877 1103 74-75 0.17307926198442658 1103 76-77 0.20693739804769606 1103 78-79 0.31349565325151474 1103 80-81 0.28081057202889426 1103 82-83 0.2737390508222006 1103 84-85 0.5095426639120788 1103 86-87 0.621322162180693 1103 88-89 0.6483810125278211 1103 90-91 0.5223423061223187 1103 92-93 0.5816808628278345 1103 94-95 0.48247760439015863 1103 96-97 0.7078609007480949 1103 98-99 0.7518974768141469 1103 100-101 0.4931908448921156 1104 1 0.09908794333333759 1104 2 0.047780618273570497 1104 3 0.01742587615751745 1104 4 0.015586873343941932 1104 5 -0.005233111031408555 1104 6 -0.08618256074964847 1104 7 -0.035855506025065154 1104 8 0.04920764116113929 1104 9 0.027056901366137254 1104 10-11 0.007308055058864227 1104 12-13 0.11077031519246106 1104 14-15 0.0029331369820582154 1104 16-17 -0.0013222479793029152 1104 18-19 0.040097583858880625 1104 20-21 -0.06295478212956596 1104 22-23 -0.03410893153518657 1104 24-25 0.08488618692165772 1104 26-27 0.08661217628061735 1104 28-29 -0.021753206090302513 1104 30-31 0.15265118061999772 1104 32-33 0.20828102344957955 1104 34-35 0.2016225743748663 1104 36-37 0.12530463725477858 1104 38-39 0.20719847204425434 1104 40-41 0.2639992953433392 1104 42-43 0.2723379630447127 1104 44-45 0.1602903789078951 1104 46-47 0.13533386379329215 1104 48-49 0.21500625743721002 1104 50-51 0.27078912513096753 1104 52-53 0.2662294515368586 1104 54-55 0.2603618218381385 1104 56-57 0.10634901472722191 1104 58-59 0.22930751505315783 1104 60-61 0.13774423427611993 1104 62-63 0.174406277062495 1104 64-65 0.158490800530231 1104 66-67 0.1431171600497052 1104 68-69 0.20105092764381993 1104 70-71 0.25134150933154586 1104 72-73 0.10755916538497345 1104 74-75 0.09519777197741774 1104 76-77 0.03627444165244853 1104 78-79 0.3091083728967057 1104 80-81 0.09811610144550897 1104 82-83 -0.11152199282753728 1104 84-85 -0.003095721463779455 1104 86-87 0.10356731377196127 1104 88-89 0.1870769002374395 1104 90-91 0.07998101016412917 1104 92-93 0.21577690124770044 1104 94-95 -0.07951557615846738 1104 96-97 -0.0015991666591261833 1104 98-99 0.14935264801274073 1104 100-101 0.09269616286450244 1105 1 0.07980085720042496 1105 2 0.09975959051983807 1105 3 0.13028454495292863 1105 4 0.11432499850606348 1105 5 0.05300545213613361 1105 6 0.05797842960549815 1105 7 0.1343831829107316 1105 8 0.06322468800021142 1105 9 -0.03973783348555315 1105 10-11 0.13085287703128046 1105 12-13 0.07185573891357677 1105 14-15 0.18530693421393352 1105 16-17 0.1963481134210241 1105 18-19 0.2256326186154638 1105 20-21 0.1891994359543432 1105 22-23 0.25440606851284997 1105 24-25 0.22829496956354234 1105 26-27 0.10149584501052544 1105 28-29 0.00986593793444257 1105 30-31 0.16976417903129715 1105 32-33 0.10160072024327604 1105 34-35 0.3139384308697899 1105 36-37 0.24656772610568112 1105 38-39 0.2654566865018353 1105 40-41 0.17562307707252955 1105 42-43 0.0413975309084762 1105 44-45 0.053070320268439275 1105 46-47 0.12534751488944096 1105 48-49 0.1666173575338945 1105 50-51 0.2470892326453651 1105 52-53 0.2989636970976335 1105 54-55 0.5408977430862478 1105 56-57 0.3193746758184517 1105 58-59 0.3404825049873281 1105 60-61 0.4504488806271425 1105 62-63 0.3823605952369675 1105 64-65 0.4101056998763184 1105 66-67 0.4986103069898249 1105 68-69 0.5514030787613606 1105 70-71 0.5601748515443532 1105 72-73 0.5938849243832927 1105 74-75 0.38337324140306706 1105 76-77 0.4769758005609894 1105 78-79 0.7197312489197749 1105 80-81 0.5037071554795709 1105 82-83 0.6264025077895141 1105 84-85 0.7264217389257688 1105 86-87 0.6617467047217112 1105 88-89 0.48157659149383747 1105 90-91 0.6208934675972522 1105 92-93 0.7208827430958671 1105 94-95 0.5710526081409348 1105 96-97 0.7391450757312406 1105 98-99 0.6454721732725126 1105 100-101 0.5130586549275655 1106 1 -0.1335440349244834 1106 2 -0.06474105548632281 1106 3 -0.045845047140325335 1106 4 -0.0689741345010475 1106 5 -0.03481422069327067 1106 6 -0.06638214121841202 1106 7 -6.154014713715128E-4 1106 8 -0.058317592205277435 1106 9 -0.06191334170907936 1106 10-11 -0.011932440964940838 1106 12-13 -0.09220477102623192 1106 14-15 -0.1932107092290849 1106 16-17 -0.17121248045365434 1106 18-19 -0.11551438265483682 1106 20-21 0.05938915380791343 1106 22-23 -0.08498748746084317 1106 24-25 -0.04962276925621012 1106 26-27 -0.006377997126783441 1106 28-29 -0.036737330764871956 1106 30-31 0.004069653505496262 1106 32-33 -0.21995479745110202 1106 34-35 -0.18676687256101587 1106 36-37 -0.11841928278139591 1106 38-39 -0.14484011720833223 1106 40-41 -0.14609509052493763 1106 42-43 -0.24585890138364874 1106 44-45 -0.08801166279926775 1106 46-47 0.04114936012022952 1106 48-49 -0.0014974987704476916 1106 50-51 -0.025519103882508887 1106 52-53 -0.17148955953342693 1106 54-55 -0.5421150926845328 1106 56-57 -0.35336160918259196 1106 58-59 -0.34323484080010047 1106 60-61 -0.3643477923784886 1106 62-63 -0.29320087055811683 1106 64-65 -0.5918283515385809 1106 66-67 -0.6723453895985827 1106 68-69 -0.7139636831985214 1106 70-71 -0.7732808346043889 1106 72-73 -0.5831088627662346 1106 74-75 -0.6196924691577763 1106 76-77 -0.5369417277952167 1106 78-79 -0.5174348594338731 1106 80-81 -0.5515203163604916 1106 82-83 -0.6575833878122168 1106 84-85 -0.45273681612506067 1106 86-87 -0.4151292813041749 1106 88-89 -0.4692348624000324 1106 90-91 -0.6276341227538289 1106 92-93 -0.376459778808627 1106 94-95 -0.3211180226734349 1106 96-97 0.05959315429093692 1106 98-99 -0.03312222255095776 1106 100-101 -0.24450466996217202 1107 1 0.10206495068450039 1107 2 0.11543912469385731 1107 3 0.1421741408788577 1107 4 0.11509629956938738 1107 5 0.09935566347660796 1107 6 0.06547699064072532 1107 7 0.06291566205969445 1107 8 0.07289803901035441 1107 9 -0.05694083673657957 1107 10-11 0.09374791471541499 1107 12-13 0.1819411281196679 1107 14-15 0.13912812310974942 1107 16-17 0.11916718492601319 1107 18-19 0.15251826037780347 1107 20-21 0.174122393541154 1107 22-23 0.25115384868048807 1107 24-25 0.1623375677041281 1107 26-27 0.09576310501432062 1107 28-29 0.13731496828743417 1107 30-31 0.12229827173410968 1107 32-33 0.20147962398331032 1107 34-35 0.02940546861132276 1107 36-37 -0.014040553402665523 1107 38-39 0.13113390876569042 1107 40-41 0.16746522303537859 1107 42-43 0.028711123186376142 1107 44-45 -0.08638828617588956 1107 46-47 0.05627162524249485 1107 48-49 0.07649770422474944 1107 50-51 0.15166294829955262 1107 52-53 0.030803642759778427 1107 54-55 0.010885960316514343 1107 56-57 0.010874380053394361 1107 58-59 0.008540635975379018 1107 60-61 0.14221883918813916 1107 62-63 0.16951996716272077 1107 64-65 0.09083808112784908 1107 66-67 0.03225856500537816 1107 68-69 0.20567186143702543 1107 70-71 0.3113541250305687 1107 72-73 0.19482491516754052 1107 74-75 0.13016128069597244 1107 76-77 0.02790558705209989 1107 78-79 0.06518184818283501 1107 80-81 0.32868618884601375 1107 82-83 0.07514446991564228 1107 84-85 0.4037332903450448 1107 86-87 0.44224757607268117 1107 88-89 0.5004621072969364 1107 90-91 0.5933498482301403 1107 92-93 0.6274451000962493 1107 94-95 0.4051017035462934 1107 96-97 0.6601401298379095 1107 98-99 0.8109604965317629 1107 100-101 0.5956731702156652 1108 1 0.04159023202739576 1108 2 0.035134585301250354 1108 3 0.0044822499077668 1108 4 -0.027308171223594968 1108 5 -0.022827262735049203 1108 6 0.04421877825196674 1108 7 0.024150731664533964 1108 8 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54-55 -0.5714930326494212 2316 56-57 -0.5167313498973698 2316 58-59 -0.6544997731566369 2316 60-61 -0.49206837402118 2316 62-63 -0.4411952523703846 2316 64-65 -0.4562412127973019 2316 66-67 -0.256846590117334 2316 68-69 -0.29503117416727775 2316 70-71 -0.7469739070358052 2316 72-73 -0.5371599870038644 2316 74-75 -0.39484441367340395 2316 76-77 -0.2517701640992449 2316 78-79 -0.3647165898752327 2316 80-81 -0.3818038166933597 2316 82-83 -0.43978471773707994 2316 84-85 -0.23080410447759547 2316 86-87 -0.4388149056480586 2316 88-89 -0.4521422074851067 2316 90-91 -0.6511960241270316 2316 92-93 -0.7213392362772275 2316 94-95 -0.4342493539890775 2316 96-97 -0.3486088138397214 2316 98-99 -0.12804421530619337 2316 100-101 -0.17772832932406502 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 14.0 5 40.0 6 106.0 7 187.0 8 441.0 9 694.0 10 1325.0 11 1974.0 12 3237.0 13 4756.0 14 6262.0 15 8355.0 16 10884.0 17 15392.0 18 15275.0 19 10353.0 20 8616.0 21 8735.0 22 9013.0 23 9722.0 24 11061.0 25 12829.0 26 15275.0 27 18679.0 28 22487.0 29 27650.0 30 33829.0 31 41352.0 32 52006.0 33 67617.0 34 96298.0 35 153898.0 36 226021.0 37 339661.0 38 271135.0 39 25362.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49743196722222 18.977961502702627 14.343742016753467 22.18086451332169 2 15.93112574126411 20.532762648457027 40.01202358976034 23.524088020518516 3 19.68739831395554 25.50988404687261 29.627180573506024 25.175537065665825 4 12.90445454539515 16.871919814393397 36.470116278537965 33.75350936167349 5 13.16834678275573 38.1540871935495 33.89319780026644 14.784368223428327 6 31.576055389275353 36.05425242837701 17.352989025506535 15.016703156841107 7 28.191126190667532 33.63093896373132 20.31911425246758 17.858820593133576 8 26.525945367101738 34.759036498811206 19.540908030679308 19.174110103407745 9 26.844263767826188 16.729748853838146 20.191069443981647 36.234917934354016 10-11 25.09106209601914 27.01249682303539 26.495721469877804 21.40071961106766 12-13 26.25312877928116 24.535509807265235 27.45635716334466 21.755004250108946 14-15 22.875125200173272 25.603753947113095 26.57177261231101 24.949348240402628 16-17 21.44126067759003 28.890419210972322 27.38007189609955 22.288248215338097 18-19 21.415060870315163 26.981298867269192 30.392558487183358 21.211081775232284 20-21 22.231565274943826 26.04039465577384 30.66834188255325 21.059698186729076 22-23 21.84660536840049 26.156104770249105 30.50866061785673 21.488629243493673 24-25 21.517781039677825 26.3993709099427 30.753441193833638 21.32940685654584 26-27 21.391900458107056 26.58677567131309 30.598904725495196 21.42241914508466 28-29 21.24486744457951 26.89401871289933 30.614253648283608 21.246860194237556 30-31 21.340447120608356 26.728089071307092 30.517023630083063 21.414440178001488 32-33 21.08571236296126 26.71057699438141 30.51089367927145 21.692816963385884 34-35 21.639911516858522 26.76183723415031 30.35641584462517 21.241835404365997 36-37 21.730517111997756 26.534597895249924 30.300514769611443 21.43437022314088 38-39 21.479732319385672 26.67967755732454 30.399903545582063 21.440686577707723 40-41 21.628252554896207 26.64022081988006 30.414194107418364 21.31733251780537 42-43 21.370883956890097 26.749069206110242 30.12439771199166 21.755649125008002 44-45 21.430683241136187 27.01278955692119 30.03678847492644 21.51973872701619 46-47 21.725113015003114 27.10573134215239 29.443487271560727 21.725668371283767 48-49 21.684310662383236 27.16665719294188 29.343817153191438 21.80521499148345 50-51 21.81576676081589 27.472691171599212 29.188448066674116 21.523094000910785 52-53 21.889367802010913 27.425583891793075 29.06329689542772 21.62175141076829 54-55 21.53792528040592 27.468771009618116 29.180444402629384 21.81285930734658 56-57 21.69561379609539 27.628942294562275 28.825147055076318 21.85029685426602 58-59 21.68966821709073 27.336465542756226 28.964182133339083 22.00968410681396 60-61 21.5694172483207 27.419703648821436 29.165972471315847 21.844906631542017 62-63 21.647885823975578 27.418266256095038 29.21579119649224 21.718056723437147 64-65 21.478338818293306 27.386349603965638 29.185083260973677 21.95022831676738 66-67 21.423815898739605 27.579515585583998 28.901753553790176 22.094914961886225 68-69 21.627209627410863 27.463981002864006 29.04495224418206 21.863857125543067 70-71 21.495130178778986 27.4511302807032 28.745241086695035 22.30849845382278 72-73 21.534005118424826 27.512905499921924 28.64103011403098 22.31205926762227 74-75 21.491177348781385 27.814235284201942 28.398633431533394 22.295953935483283 76-77 21.526360802561694 27.473344531929396 28.781012564772514 22.2192821007364 78-79 21.505812620177466 27.495101430924457 28.796529872614336 22.202556076283738 80-81 21.458934016486893 27.73334927532539 28.564848299531736 22.24286840865598 82-83 21.495848875142187 27.750042631761545 28.499479598497008 22.254628894599257 84-85 21.547726972023895 27.556603141778595 28.437849660487323 22.457820225710183 86-87 21.537440282670737 27.52233846266425 28.418131069737296 22.522090184927713 88-89 21.58021884325707 27.521687500714613 28.3324104609625 22.56568319506582 90-91 21.45642095813489 27.54663121963299 28.51063329340206 22.48631452883006 92-93 21.39259681283152 27.485269121720485 28.48942222158338 22.632711843864612 94-95 21.265637362802497 27.407845158828632 28.51862305617135 22.807894422197524 96-97 21.33561225416501 27.132355775607316 28.46325076818841 23.06878120203927 98-99 21.240482990090484 27.188642768052507 28.33529014752223 23.235584094334776 100-101 21.450374996161507 27.038892580374757 28.519733768732657 22.99099865473108 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4648.0 1 2954.0 2 2887.5 3 3713.0 4 2163.0 5 1657.0 6 2876.0 7 5379.0 8 7483.0 9 7600.5 10 5526.0 11 3378.5 12 2886.0 13 2986.5 14 2963.0 15 2858.5 16 2849.0 17 2924.0 18 3030.5 19 3256.0 20 3582.0 21 4048.0 22 4664.0 23 5504.5 24 6641.5 25 8088.0 26 9899.0 27 12051.5 28 14082.0 29 15954.5 30 18221.0 31 21015.5 32 24250.0 33 26424.5 34 27876.0 35 29736.0 36 32916.0 37 36099.0 38 37967.0 39 39451.0 40 40703.0 41 42447.5 42 43545.0 43 44778.5 44 47032.5 45 49720.0 46 51549.5 47 53481.0 48 59375.0 49 60059.0 50 55892.0 51 53726.5 52 51419.5 53 50924.0 54 50957.0 55 48230.0 56 43628.0 57 39466.5 58 36128.0 59 32166.5 60 26999.5 61 22714.0 62 19020.0 63 14396.5 64 10094.0 65 7493.5 66 5808.0 67 4644.0 68 3592.5 69 2670.5 70 2043.0 71 1467.5 72 1086.0 73 923.0 74 725.0 75 524.5 76 365.0 77 234.5 78 160.5 79 86.0 80 38.0 81 21.5 82 14.0 83 11.0 84 5.0 85 4.0 86 7.0 87 4.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014635271396080753 3 0.006729611400876418 4 0.0 5 0.0 6 0.0 7 0.0 8 3.920161981093059E-4 9 3.920161981093059E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.5735223112752354E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005520894790039391 26-27 0.022148915193175783 28-29 3.2668016509108824E-5 30-31 0.0 32-33 3.5934818160019707E-4 34-35 0.02051551436772034 36-37 0.003822157931565732 38-39 0.019110789657828663 40-41 0.016464680320590848 42-43 0.008820364457459382 44-45 0.00277678140327425 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.6334008254554412E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.003038125535347121 86-87 3.2668016509108824E-4 88-89 9.800404952732647E-5 90-91 1.6334008254554412E-4 92-93 3.5934818160019707E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1530549.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.06879800775503 #Duplication Level Percentage of deduplicated Percentage of total 1 78.54911527618803 47.18350939213204 2 13.053390754914467 15.682029851465082 3 3.8431792180616946 6.925654684720496 4 1.60420864682777 3.854515406743654 5 0.8064689425563105 2.4221810004971407 6 0.47905555724345417 1.7265774901529745 7 0.31543624721583563 1.3263513352832617 8 0.21923202143526074 1.05352032099412 9 0.1634820021286407 0.8838150627391828 >10 0.8825210975540666 9.501133067441797 >50 0.05442602188217917 2.244337553847985 >100 0.026232406057539195 3.0707635645336433 >500 0.0018426911630843063 0.7630326604906438 >1k 0.001083935978284886 1.4591965999023653 >5k 2.1678719565697724E-4 1.105840660958537 >10k+ 1.0839359782848862E-4 0.7975413480970984 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12249 0.8003010684401479 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9953 0.6502895366303202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7031 0.4593776481510882 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4663 0.30466192196394887 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4167 0.27225524958691294 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2505 0.16366676271063518 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2468 0.16124932948896115 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1992 0.13014937777228955 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1646 0.10754311034798625 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1551 0.10133618721125558 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.533603301821765E-5 2 0.0 0.0 0.0 0.0 6.533603301821765E-5 3 0.0 0.0 0.0 0.0 6.533603301821765E-5 4 0.0 0.0 0.0 0.0 6.533603301821765E-5 5 0.0 0.0 0.0 0.0 6.533603301821765E-5 6 0.0 0.0 0.0 0.0 6.533603301821765E-5 7 0.0 0.0 0.0 0.0 6.533603301821765E-5 8 0.0 0.0 0.0 0.0 6.533603301821765E-5 9 0.0 0.0 0.0 0.0 6.533603301821765E-5 10-11 0.0 0.0 0.0 0.0 6.533603301821765E-5 12-13 0.0 0.0 0.0 6.533603301821765E-5 6.533603301821765E-5 14-15 0.0 0.0 0.0 6.533603301821765E-5 6.533603301821765E-5 16-17 0.0 0.0 0.0 9.800404952732647E-5 6.533603301821765E-5 18-19 0.0 0.0 0.0 1.306720660364353E-4 6.533603301821765E-5 20-21 0.0 0.0 0.0 1.306720660364353E-4 6.533603301821765E-5 22-23 0.0 0.0 0.0 4.900202476366324E-4 6.533603301821765E-5 24-25 0.0 0.0 0.0 0.0010780445448005911 6.533603301821765E-5 26-27 0.0 0.0 0.0 0.0021560890896011823 6.533603301821765E-5 28-29 0.0 0.0 0.0 0.006141587103712459 6.533603301821765E-5 30-31 0.0 0.0 0.0 0.016334008254554413 6.533603301821765E-5 32-33 0.0 0.0 0.0 0.03603282220954703 6.533603301821765E-5 34-35 0.0 0.0 0.0 0.0662507374804727 6.533603301821765E-5 36-37 0.0 3.2668016509108824E-5 0.0 0.11963027645635652 6.533603301821765E-5 38-39 0.0 6.533603301821765E-5 0.0 0.19558341484003453 6.533603301821765E-5 40-41 0.0 6.533603301821765E-5 0.0 0.27702477999724284 6.533603301821765E-5 42-43 0.0 1.306720660364353E-4 0.0 0.3562447200318317 6.533603301821765E-5 44-45 0.0 1.306720660364353E-4 0.0 0.4504592796441016 9.800404952732647E-5 46-47 0.0 1.306720660364353E-4 0.0 0.5686521633740573 1.306720660364353E-4 48-49 0.0 1.306720660364353E-4 0.0 0.6896544965237964 1.306720660364353E-4 50-51 0.0 1.306720660364353E-4 0.0 0.813498947109828 1.9600809905465295E-4 52-53 0.0 1.306720660364353E-4 0.0 0.9287190413374546 1.9600809905465295E-4 54-55 0.0 1.306720660364353E-4 0.0 1.0543275648149781 1.9600809905465295E-4 56-57 0.0 1.306720660364353E-4 0.0 1.1991122139833483 1.9600809905465295E-4 58-59 0.0 1.306720660364353E-4 0.0 1.3564413814912166 1.9600809905465295E-4 60-61 0.0 1.306720660364353E-4 0.0 1.5287651685767656 1.9600809905465295E-4 62-63 0.0 1.306720660364353E-4 0.0 1.69393466004682 1.9600809905465295E-4 64-65 0.0 1.306720660364353E-4 0.0 1.8597575118470562 1.9600809905465295E-4 66-67 0.0 1.306720660364353E-4 0.0 2.0491013355338508 1.9600809905465295E-4 68-69 0.0 1.306720660364353E-4 0.0 2.2582746452416744 1.9600809905465295E-4 70-71 0.0 1.306720660364353E-4 0.0 2.481495202048415 1.9600809905465295E-4 72-73 0.0 1.306720660364353E-4 0.0 2.6998482243952986 2.2867611556376177E-4 74-75 0.0 1.306720660364353E-4 0.0 2.914575096909671 2.940121485819794E-4 76-77 0.0 1.306720660364353E-4 0.0 3.14151980759845 3.2668016509108824E-4 78-79 0.0 1.306720660364353E-4 0.0 3.397049032732699 3.920161981093059E-4 80-81 0.0 1.306720660364353E-4 0.0 3.6678668895932116 3.920161981093059E-4 82-83 0.0 1.306720660364353E-4 0.0 3.9464924023994006 3.920161981093059E-4 84-85 0.0 1.306720660364353E-4 0.0 4.224660562974462 3.920161981093059E-4 86-87 0.0 1.306720660364353E-4 0.0 4.512008436188584 3.920161981093059E-4 88-89 0.0 1.306720660364353E-4 0.0 4.81124746741202 3.920161981093059E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7655 0.0 33.69778 1 GTATCAA 10965 0.0 28.637787 1 GTACATG 8625 0.0 25.556744 1 TACATGG 8485 0.0 25.418543 2 ACATGGG 8615 0.0 23.76358 3 TATCAAC 13345 0.0 23.56601 2 ATCAACG 13350 0.0 23.447365 3 TCAACGC 13390 0.0 23.375793 4 CAACGCA 13485 0.0 23.175892 5 AACGCAG 13835 0.0 22.658245 6 GAGTACT 8490 0.0 22.602129 12-13 CATGGGG 3120 0.0 20.703598 4 ACGCAGA 15235 0.0 20.482567 7 TACCTGG 1160 0.0 20.476637 2 ATGGGAT 2020 0.0 20.456406 5 GTACTTT 9410 0.0 20.367119 14-15 CATGGGA 5550 0.0 20.196707 4 CGCAGAG 15450 0.0 20.166794 8 AGTACTT 8895 0.0 19.94437 12-13 GTGTAGC 1135 0.0 19.253452 1 >>END_MODULE