FastQCFastQC Report
Thu 26 May 2016
SRR937924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583004
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130060.8216024722615989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102360.646618707217417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68590.43329012434586395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47380.29930436057015647No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44530.2813006157912425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.15413732372122876No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23450.14813607546159074No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.13461747411882724No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18160.11471859831055387No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15860.10018926041879869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75850.034.5167431
GTATCAA110550.030.9594231
GTACATG87200.027.2945651
TACATGG85850.027.1963082
TATCAAC131100.026.00672
ATCAACG132200.025.6823943
TCAACGC132700.025.5856294
ACATGGG88400.025.551133
CAACGCA133950.025.3468675
AACGCAG136600.024.8836526
GAGTACT86300.023.70926512-13
CATGGGA53850.023.0474854
TAGCCCT10000.022.8249594
ATGGGAT19350.022.3629555
ACGCAGA152150.022.3036067
CGCAGAG154000.022.034288
CATGGGG32000.021.8441984
GTACTTT92550.021.74626214-15
TGACGCG905.169826E-621.11283394-95
GCAGAGT161100.020.7675259
Produced by FastQC (version 0.11.5)