##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937924_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1583004 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20432102508901 33.0 31.0 34.0 31.0 34.0 2 32.312593651058364 34.0 31.0 34.0 31.0 34.0 3 32.33555000492734 34.0 31.0 34.0 31.0 34.0 4 35.84535225432153 37.0 35.0 37.0 35.0 37.0 5 35.863220181376676 37.0 35.0 37.0 35.0 37.0 6 35.90659973063871 37.0 37.0 37.0 35.0 37.0 7 35.8522183140409 37.0 36.0 37.0 35.0 37.0 8 35.85114187961622 37.0 36.0 37.0 35.0 37.0 9 37.52358364223969 39.0 38.0 39.0 35.0 39.0 10-11 37.4503851537962 39.0 37.5 39.0 35.0 39.0 12-13 37.311076914524534 39.0 37.0 39.0 35.0 39.0 14-15 38.53041937986259 40.0 38.0 41.0 34.5 41.0 16-17 38.36723438475203 40.0 38.0 41.0 34.0 41.0 18-19 38.554786658782916 40.0 38.0 41.0 34.0 41.0 20-21 38.5616514550816 40.0 38.0 41.0 34.0 41.0 22-23 38.439517840763514 40.0 38.0 41.0 34.0 41.0 24-25 38.32567321371266 40.0 38.0 41.0 34.0 41.0 26-27 38.14569988452335 40.0 38.0 41.0 34.0 41.0 28-29 37.974693367799446 40.0 38.0 41.0 33.0 41.0 30-31 37.745471268550176 40.0 37.5 41.0 33.0 41.0 32-33 37.43884317411706 40.0 36.5 41.0 33.0 41.0 34-35 37.20148812005529 40.0 36.0 41.0 32.0 41.0 36-37 36.949816930342564 39.5 36.0 41.0 31.0 41.0 38-39 36.663536857771675 39.0 35.0 41.0 30.5 41.0 40-41 36.54638649049529 39.0 35.0 41.0 30.0 41.0 42-43 36.33613275771887 39.0 35.0 41.0 30.0 41.0 44-45 36.001325960010206 39.0 35.0 41.0 29.5 41.0 46-47 35.59274992356305 39.0 35.0 40.0 27.5 41.0 48-49 35.17656493603301 39.0 35.0 40.0 26.0 41.0 50-51 34.44079642249798 37.5 34.0 39.5 24.5 40.5 52-53 34.51290015691685 38.0 34.0 40.0 24.5 40.5 54-55 34.61599844346571 38.0 34.0 40.0 24.5 41.0 56-57 34.332906297141385 38.0 34.0 40.0 23.5 41.0 58-59 33.95393283908316 38.0 34.0 40.0 22.5 41.0 60-61 33.492905892846764 37.0 33.0 40.0 21.0 41.0 62-63 32.98266934259168 36.5 32.5 39.5 18.5 41.0 64-65 32.47776663861873 36.0 32.0 39.0 14.5 41.0 66-67 31.949313141343925 35.0 31.0 39.0 9.0 40.0 68-69 31.32011447854838 35.0 31.0 38.5 6.5 40.0 70-71 30.76755175602841 35.0 30.0 37.5 2.0 40.0 72-73 30.101202523809164 34.5 29.0 37.0 2.0 39.0 74-75 29.465181705163094 34.0 29.0 36.0 2.0 39.0 76-77 28.755198976123875 34.0 27.5 36.0 2.0 38.5 78-79 28.032431061450254 34.0 26.0 35.0 2.0 37.5 80-81 27.352741370205003 33.0 25.5 35.0 2.0 37.0 82-83 26.652655647111445 33.0 24.5 35.0 2.0 36.0 84-85 25.958345651685022 32.0 22.0 35.0 2.0 36.0 86-87 25.24328523490781 32.0 19.0 35.0 2.0 35.0 88-89 24.413053915214363 31.0 13.5 34.0 2.0 35.0 90-91 23.549299622742584 31.0 4.5 34.0 2.0 35.0 92-93 22.65444338738247 30.0 2.0 34.0 2.0 35.0 94-95 21.611347160209323 29.0 2.0 34.0 2.0 35.0 96-97 20.391546389017336 29.0 2.0 34.0 2.0 35.0 98-99 18.88560768008167 26.0 2.0 34.0 2.0 35.0 100-101 14.804066824846934 10.0 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21782486702848303 1101 2 0.203252312126601 1101 3 0.1866164108244064 1101 4 0.009319335854833355 1101 5 0.13241108458900896 1101 6 0.1382754989548971 1101 7 0.23414131743219713 1101 8 0.24507502615448828 1101 9 0.19039893869711477 1101 10-11 0.24512191494833502 1101 12-13 0.31255537439627545 1101 14-15 0.34387367693302195 1101 16-17 0.3869441141868464 1101 18-19 0.3085917545973018 1101 20-21 0.551722614281374 1101 22-23 0.47257454925976816 1101 24-25 0.5680055976979403 1101 26-27 0.3431963984302797 1101 28-29 0.5141621044227449 1101 30-31 0.22623383964055677 1101 32-33 0.24307704636882477 1101 34-35 0.3273706652050379 1101 36-37 0.20706436868123035 1101 38-39 0.17222139155064298 1101 40-41 0.2814476092513303 1101 42-43 0.4154776993909124 1101 44-45 0.31315924692486874 1101 46-47 0.26473897924811496 1101 48-49 0.2044926658738362 1101 50-51 0.28051315525880227 1101 52-53 0.284719896284777 1101 54-55 0.2936049807464869 1101 56-57 0.20152712682070018 1101 58-59 0.26460385134247844 1101 60-61 0.3640169241469948 1101 62-63 0.10811666009860943 1101 64-65 -0.08872337798455021 1101 66-67 -0.24471817738971424 1101 68-69 -0.05998111171122744 1101 70-71 -0.13455261881195923 1101 72-73 -0.1813545375110266 1101 74-75 -0.22367111483985624 1101 76-77 -0.27020288830698647 1101 78-79 -0.428585645378476 1101 80-81 -0.49548707090307786 1101 82-83 -0.5059321565753336 1101 84-85 -0.3613120001865191 1101 86-87 -0.32833806837127 1101 88-89 -0.0788293662338937 1101 90-91 -0.37067016810905784 1101 92-93 -0.5060016828065699 1101 94-95 -0.7348839452845048 1101 96-97 -0.7376868262627774 1101 98-99 -0.9885029878086797 1101 100-101 -1.1654479977066075 1102 1 0.13856818816527294 1102 2 0.12794029769123938 1102 3 0.11904335684214828 1102 4 0.05644729936543058 1102 5 0.10137254015514685 1102 6 0.1017660508069369 1102 7 0.1062905831331662 1102 8 0.14886180375962255 1102 9 0.18547938579249745 1102 10-11 0.22425704601945995 1102 12-13 0.1874977331752845 1102 14-15 0.22318925008725188 1102 16-17 0.17524670553564192 1102 18-19 0.2816068916831611 1102 20-21 0.14852979186645854 1102 22-23 0.1828146374759001 1102 24-25 0.07632097036096042 1102 26-27 0.11206763295486155 1102 28-29 0.03266618943101207 1102 30-31 0.12025544256772491 1102 32-33 0.04923087324466735 1102 34-35 0.11544519780991891 1102 36-37 0.13073486138836188 1102 38-39 0.01365164567324939 1102 40-41 0.02388533167125928 1102 42-43 0.11003812242734767 1102 44-45 0.13874818983499893 1102 46-47 0.05670304119988856 1102 48-49 -0.187362378986073 1102 50-51 -0.03182206603336368 1102 52-53 -0.09959166704538092 1102 54-55 0.08943225757472106 1102 56-57 0.085748469109312 1102 58-59 -0.027257458867538276 1102 60-61 -0.03765216512171321 1102 62-63 -0.1396090178122762 1102 64-65 -0.29344995810584606 1102 66-67 -0.3081435696006345 1102 68-69 -0.662608799546426 1102 70-71 -0.7718402234433057 1102 72-73 -0.7566815887866518 1102 74-75 -0.5753740369991434 1102 76-77 -0.7112171231824291 1102 78-79 -0.7349775555584195 1102 80-81 -0.5468428926664011 1102 82-83 -0.7975553834812565 1102 84-85 -0.7169432968633771 1102 86-87 -0.757236369208325 1102 88-89 -0.8721642867892534 1102 90-91 -1.0493830145829186 1102 92-93 -1.3159498057076782 1102 94-95 -1.4362034280172011 1102 96-97 -1.6640251050457948 1102 98-99 -1.7235889484121572 1102 100-101 -1.9105697961127035 1103 1 0.04986975015776807 1103 2 0.12654513425780323 1103 3 0.17768106710090592 1103 4 0.08641993743001564 1103 5 0.09458471326367857 1103 6 0.09812041739781563 1103 7 0.18474699596374933 1103 8 0.16973413769334655 1103 9 0.10301809112020521 1103 10-11 0.08899189114893602 1103 12-13 0.19123563929824883 1103 14-15 0.2619883268679928 1103 16-17 0.24360060953265616 1103 18-19 0.18239650005315866 1103 20-21 0.1659607226643871 1103 22-23 0.17369563274169053 1103 24-25 0.02241992607989829 1103 26-27 0.09627005222239404 1103 28-29 0.12535105473745745 1103 30-31 0.10395466796904884 1103 32-33 0.16435350983289254 1103 34-35 0.10366750861394536 1103 36-37 -0.008523118355931558 1103 38-39 -0.16902692048260093 1103 40-41 -0.09901823227004058 1103 42-43 0.10996504243798455 1103 44-45 0.13404178930744592 1103 46-47 0.1151146217945751 1103 48-49 -0.08028666122738315 1103 50-51 -0.04082000896699611 1103 52-53 -0.08908783342169357 1103 54-55 -0.09659100991280667 1103 56-57 -0.2728287102491649 1103 58-59 -0.021257763982646338 1103 60-61 -0.18064288371699888 1103 62-63 -0.325357927763946 1103 64-65 -0.2931261423953728 1103 66-67 -0.30894671130199214 1103 68-69 -0.18791096192671475 1103 70-71 -0.41825438275276383 1103 72-73 -0.4324411777670747 1103 74-75 -0.5233856215307959 1103 76-77 -0.6521150966275719 1103 78-79 -0.7054544701557148 1103 80-81 -0.6924615381521733 1103 82-83 -1.0996535419639457 1103 84-85 -1.343534570571311 1103 86-87 -1.2342046077099127 1103 88-89 -1.2966455911071257 1103 90-91 -1.3114611437351904 1103 92-93 -1.4854931665013709 1103 94-95 -1.5931283106463106 1103 96-97 -1.63984891583506 1103 98-99 -1.436738425287384 1103 100-101 -1.6833094837142024 1104 1 0.2570128386065349 1104 2 0.11562226592779012 1104 3 0.18289517155852053 1104 4 0.2556333491767262 1104 5 0.24630060417391064 1104 6 0.2761377342318596 1104 7 0.18265102922138254 1104 8 0.25307533207525523 1104 9 0.3230246709351903 1104 10-11 0.3223432462294511 1104 12-13 0.40848662826509496 1104 14-15 0.5011049984390183 1104 16-17 0.4614788397783087 1104 18-19 0.5120278661532254 1104 20-21 0.4037109797324021 1104 22-23 0.5953611049982968 1104 24-25 0.49468380012665847 1104 26-27 0.34109032518615834 1104 28-29 0.3501221339632181 1104 30-31 0.4865157647425491 1104 32-33 0.4799710902349972 1104 34-35 0.49847119124535055 1104 36-37 0.5021132323528974 1104 38-39 0.4081202369387711 1104 40-41 0.4556348279775051 1104 42-43 0.5444531755398501 1104 44-45 0.5648605726611677 1104 46-47 0.4830722368979252 1104 48-49 0.6035314054803038 1104 50-51 0.6245093408093041 1104 52-53 0.6993971618311647 1104 54-55 0.6226301690192102 1104 56-57 0.44956481243453794 1104 58-59 0.6613928783471579 1104 60-61 0.5870037623842279 1104 62-63 0.6044131068767626 1104 64-65 0.5557455507430831 1104 66-67 0.4157527971830106 1104 68-69 0.5277990268613166 1104 70-71 0.5855205631702631 1104 72-73 0.5536349106127005 1104 74-75 0.46548829616707366 1104 76-77 0.18194943155229382 1104 78-79 0.03781565096074857 1104 80-81 -0.03180775234157451 1104 82-83 -0.11337869811722356 1104 84-85 -0.1726485154144406 1104 86-87 -0.15493262059170476 1104 88-89 -0.2506891971648706 1104 90-91 -0.3409351480688585 1104 92-93 -0.4523465272052327 1104 94-95 -0.564026004848234 1104 96-97 -0.8622281243706098 1104 98-99 -0.937531569889849 1104 100-101 -1.374184425460312 1105 1 0.25953369661051084 1105 2 0.26362438930430443 1105 3 0.3368330184860113 1105 4 0.1982352847160911 1105 5 0.19534608387542818 1105 6 0.16548887936951218 1105 7 0.17751026864598174 1105 8 0.2669029093843136 1105 9 0.3047157613550624 1105 10-11 0.3008112613502547 1105 12-13 0.27725822367325037 1105 14-15 0.27291913001364065 1105 16-17 0.25134449537949877 1105 18-19 0.24151934180432733 1105 20-21 0.2932376248930737 1105 22-23 0.3226940172474144 1105 24-25 0.3046120623202171 1105 26-27 0.352312335321713 1105 28-29 0.3653215654126498 1105 30-31 0.2864539922096938 1105 32-33 0.32867942744178436 1105 34-35 0.16354069186089504 1105 36-37 0.28588308579300303 1105 38-39 0.3182181781149893 1105 40-41 0.33355544810075344 1105 42-43 0.5232348317909086 1105 44-45 0.3731600295667903 1105 46-47 0.4570004921409847 1105 48-49 0.3579977507444525 1105 50-51 0.22556455641803552 1105 52-53 0.35309586825699313 1105 54-55 0.3251072388986316 1105 56-57 0.2397460639929747 1105 58-59 0.19282845115924374 1105 60-61 0.08033063465235557 1105 62-63 0.071866757928845 1105 64-65 0.07247300528901235 1105 66-67 0.08361161870904255 1105 68-69 -0.008024046526362127 1105 70-71 -0.023566167724780485 1105 72-73 -0.23754178228919187 1105 74-75 -0.309007858036626 1105 76-77 -0.3726336820440075 1105 78-79 -0.47264723469403336 1105 80-81 -0.586235782996738 1105 82-83 -0.5790801725780597 1105 84-85 -0.6209281373422151 1105 86-87 -0.456562207810439 1105 88-89 -0.5355787320637475 1105 90-91 -0.5872672410282682 1105 92-93 -0.6708060453447224 1105 94-95 -0.7863150290594199 1105 96-97 -0.9848170624825237 1105 98-99 -1.186903636020343 1105 100-101 -1.3748825158984275 1106 1 0.166031975866602 1106 2 0.2700191388976805 1106 3 0.3130837184145676 1106 4 0.23196057804246806 1106 5 0.25891430817620886 1106 6 0.28034198401814336 1106 7 0.2993819672588174 1106 8 0.053023590399860154 1106 9 0.2909925643664337 1106 10-11 0.3588979761775235 1106 12-13 0.3834312947436942 1106 14-15 0.37565554036624604 1106 16-17 -0.10847027295219647 1106 18-19 0.2927596290026031 1106 20-21 0.3361780695925134 1106 22-23 0.4275417063568412 1106 24-25 0.4728914379425717 1106 26-27 0.45191918004837106 1106 28-29 0.40895251434567825 1106 30-31 0.34172881013632406 1106 32-33 0.485838328924018 1106 34-35 0.38698363239754485 1106 36-37 0.40934933429545595 1106 38-39 0.5252733660452336 1106 40-41 0.5015861134765203 1106 42-43 0.328676069512845 1106 44-45 0.5013218907469366 1106 46-47 0.48907813754913576 1106 48-49 0.6388035719433915 1106 50-51 0.31135740882574225 1106 52-53 0.35702765085205357 1106 54-55 0.23213374010172316 1106 56-57 0.24825485068233633 1106 58-59 0.2683998181277616 1106 60-61 0.3946380938450247 1106 62-63 0.21553002540223787 1106 64-65 0.3871390485710364 1106 66-67 0.40481377646581507 1106 68-69 0.4306389956826635 1106 70-71 0.34202180444541597 1106 72-73 0.14873649183638804 1106 74-75 0.2736832419982207 1106 76-77 0.02920576238971151 1106 78-79 -0.09274830943490997 1106 80-81 0.11717104168598524 1106 82-83 0.11770158119235674 1106 84-85 0.34553505583990685 1106 86-87 0.3744777963822372 1106 88-89 0.09605472879679766 1106 90-91 0.24046252632421528 1106 92-93 0.28536279627236283 1106 94-95 0.25122136952427 1106 96-97 0.28636905093486575 1106 98-99 -0.3045012881220579 1106 100-101 -1.0229768253528206 1107 1 -0.11020517778893435 1107 2 -0.05085376352316473 1107 3 0.07992470680989072 1107 4 0.03511316726766722 1107 5 -0.024861425207525656 1107 6 -0.07202721443591997 1107 7 0.050629130778347076 1107 8 0.02505178122105889 1107 9 0.053493478678191764 1107 10-11 5.90507825400266E-4 1107 12-13 -0.04009545833547179 1107 14-15 -0.0416365578238711 1107 16-17 -0.04204451950645449 1107 18-19 0.005164926387521973 1107 20-21 -0.031812224538931844 1107 22-23 0.0705239989391444 1107 24-25 0.1171216339498713 1107 26-27 0.06048825783442169 1107 28-29 0.07208877491417098 1107 30-31 0.09369156794581812 1107 32-33 0.11189750360385631 1107 34-35 0.07591997389055649 1107 36-37 0.022568612257643395 1107 38-39 0.025397881565702107 1107 40-41 0.0053695672453173415 1107 42-43 0.054130128359432206 1107 44-45 -0.0019580044993574575 1107 46-47 0.041679497199424986 1107 48-49 -0.13642857868276081 1107 50-51 -0.13179776795458764 1107 52-53 0.0885315996218452 1107 54-55 0.03207915621822366 1107 56-57 -0.07394269393079611 1107 58-59 -0.11936540098784576 1107 60-61 -0.1585351070325416 1107 62-63 -0.14380274052929565 1107 64-65 -0.11977399393294519 1107 66-67 -0.10044610581733693 1107 68-69 -0.21004430490457082 1107 70-71 -0.13571296016105805 1107 72-73 -0.12278534996167423 1107 74-75 -0.29160326842578144 1107 76-77 -0.49567577874459445 1107 78-79 -0.4962881017624703 1107 80-81 -0.5916351440928658 1107 82-83 -0.6082710456876868 1107 84-85 -0.6521118333649554 1107 86-87 -0.5808989275446557 1107 88-89 -0.5767203860259578 1107 90-91 -0.7854882839145532 1107 92-93 -0.6734166472087963 1107 94-95 -0.8350478030119639 1107 96-97 -1.091444439613582 1107 98-99 -1.2651989893837445 1107 100-101 -1.4170496328966422 1108 1 -0.10970002689431624 1108 2 -0.06366486211374678 1108 3 0.021150954350275697 1108 4 0.03526975331993043 1108 5 0.12262118587562298 1108 6 0.11870639479393219 1108 7 0.08466443199557006 1108 8 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-0.04301134794786776 2316 80-81 -0.005311844931018328 2316 82-83 -0.12465316317900843 2316 84-85 -0.03491575349635312 2316 86-87 0.15920660380692553 2316 88-89 0.2723324397359477 2316 90-91 0.27389850424980366 2316 92-93 0.15703732383850522 2316 94-95 0.36738640819733703 2316 96-97 0.3790389641924783 2316 98-99 0.5740909437382058 2316 100-101 0.2941164120937003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4350.0 3 1163.0 4 984.0 5 1091.0 6 1331.0 7 1522.0 8 2053.0 9 2613.0 10 3316.0 11 4427.0 12 6098.0 13 7909.0 14 9321.0 15 11892.0 16 14603.0 17 19388.0 18 18757.0 19 14704.0 20 14725.0 21 15487.0 22 17154.0 23 19025.0 24 21240.0 25 24392.0 26 27804.0 27 32078.0 28 37118.0 29 43820.0 30 51533.0 31 62404.0 32 77177.0 33 97244.0 34 129690.0 35 182079.0 36 216878.0 37 241585.0 38 136152.0 39 9897.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44051854588519 18.43549301334575 13.831222069831114 22.292766370937947 2 15.641280557597344 20.21145013610649 40.68215674588252 23.465112560413647 3 19.584676927009095 25.474093236385816 29.29031049904185 25.650919337563245 4 12.536345966159116 16.424360225605902 36.77797477145606 34.26131903677893 5 13.26097702535319 38.041999824046066 33.79347185591525 14.903551294685494 6 31.57088549426984 35.767960473097425 17.630322694169074 15.03083133846366 7 28.285590629302664 33.57767828217381 20.408803757997894 17.727927330525635 8 26.57358350775626 34.518987813890156 19.63571262855723 19.27171604979635 9 27.03354502554917 16.71457728916322 20.299620067182396 35.95225761810522 10-11 25.063797106389007 26.883188454644536 26.656203235084785 21.396811203881672 12-13 26.300712427079187 24.531317120202853 27.35961023703495 21.808360215683013 14-15 22.999076748776666 25.556821067966663 26.570974107531725 24.873128075724946 16-17 21.583532932521926 28.784000521229835 27.28720428192841 22.345262264319825 18-19 21.49765092658335 26.924065865773105 30.31792743885372 21.26035576878982 20-21 22.424404010195325 26.01961958238887 30.534932022198458 21.021044385217348 22-23 22.02253029583316 26.16358056826068 30.332827730295957 21.4810614056102 24-25 21.545795613058587 26.37935056504051 30.647595962562963 21.427257859337942 26-27 21.412787262336007 26.507720007237307 30.546884983722382 21.532607746704304 28-29 21.322097223113868 26.85550306176308 30.520921915949284 21.301477799173767 30-31 21.419831930647682 26.649931393812164 30.423461419294462 21.506775256245692 32-33 21.107456070999838 26.75222665099799 30.36435992243247 21.775957355569695 34-35 21.695446585879928 26.820855079899957 30.228099077404973 21.255599256815135 36-37 21.808185435203328 26.517279597486947 30.13224055946178 21.542294407847944 38-39 21.539501468518342 26.71154912094309 30.248254495145073 21.5006949153935 40-41 21.683314697399418 26.672003559895302 30.292104832342964 21.352576910362316 42-43 21.39981113017227 26.828933745121812 29.963521828118346 21.807733296587575 44-45 21.51765782077229 27.0824663598489 29.829322741642695 21.570553077736122 46-47 21.76861117150644 27.177739658493916 29.318915563960342 21.73473360603931 48-49 21.758039175536588 27.181899420721805 29.219105689515484 21.840955714226126 50-51 21.887808858412914 27.51733867119382 29.033085844198776 21.561766626194494 52-53 21.85899459139419 27.495741895858668 28.996464928418668 21.64879858432847 54-55 21.5319224242271 27.51016783645505 29.072617471297786 21.88529226802006 56-57 21.78194173174512 27.674514415412332 28.74283526172818 21.800708591114365 58-59 21.77515077126883 27.329954910826583 28.85700778284387 22.037886535060718 60-61 21.6187240625551 27.411080419587048 29.124255172322272 21.845940345535585 62-63 21.755775126993065 27.31717707229235 29.18312346374259 21.743924336972 64-65 21.52150200585959 27.389368059435128 29.16114749830001 21.927982436405276 66-67 21.51714745691215 27.525479855470646 28.923218384993465 22.034154302623744 68-69 21.77441683175702 27.420096057933126 28.9713659367292 21.834121173580655 70-71 21.64955523335554 27.340855453684732 28.73507279206259 22.27451652089714 72-73 21.69084569778418 27.43177891663488 28.612704980847752 22.264670404733184 74-75 21.625527940143925 27.71430593764002 28.459694538872764 22.200471583343287 76-77 21.800368872890495 27.420309744550153 28.62056829677145 22.158753085787904 78-79 21.726708937942103 27.446726503584213 28.641000609742612 22.185563948731073 80-81 21.78997313261569 27.640263922132608 28.396967498805754 22.172795446445946 82-83 21.803220734318327 27.621020799588536 28.304399294475125 22.27135917161801 84-85 21.90654850437422 27.442373136233307 28.26224724865766 22.388831110734817 86-87 21.954340300046567 27.45818487180443 28.246307865243796 22.341166962905206 88-89 21.975949888384143 27.4439732020808 28.1192309552187 22.460845954316355 90-91 21.98436468406847 27.46595821644579 28.12605135547388 22.423625744011858 92-93 21.975332993835618 27.371386791262957 28.12147443161792 22.5318057832835 94-95 21.89258643469905 27.260040585903667 28.133459347717515 22.713913631679763 96-97 22.106230313174994 26.934389576468163 28.067320328782223 22.892059781574616 98-99 22.02397882369895 26.994948633059153 27.864242617305862 23.116829925936038 100-101 22.949672846461905 26.097835811903398 27.88491735060189 23.06757399103281 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4633.0 1 2964.5 2 2989.5 3 3816.5 4 2195.5 5 1720.5 6 2951.0 7 5290.5 8 7523.5 9 7783.0 10 5480.0 11 3376.0 12 2985.5 13 2964.5 14 2986.5 15 2968.5 16 2983.0 17 3112.0 18 3211.0 19 3389.5 20 3683.5 21 4165.0 22 4894.0 23 5833.5 24 6962.0 25 8380.0 26 10308.0 27 12625.5 28 14718.0 29 16599.5 30 18777.0 31 21471.0 32 24691.0 33 27043.5 34 28759.5 35 30905.5 36 34004.0 37 37206.0 38 39061.5 39 40304.0 40 41738.5 41 43570.5 42 44865.0 43 46098.5 44 48042.5 45 50587.0 46 52565.5 47 54845.0 48 59864.5 49 60902.5 50 57761.0 51 55371.5 52 53222.0 53 52453.0 54 52023.0 55 49461.5 56 45302.5 57 41378.5 58 37937.0 59 33837.0 60 28688.5 61 24303.0 62 20295.5 63 15560.5 64 11383.5 65 8724.5 66 6724.5 67 5248.0 68 4148.0 69 3144.0 70 2378.0 71 1792.5 72 1363.5 73 1080.5 74 860.0 75 654.5 76 479.0 77 334.5 78 225.0 79 125.0 80 74.0 81 52.0 82 36.5 83 23.0 84 15.5 85 16.0 86 14.5 87 9.0 88 6.0 89 6.0 90 3.5 91 2.5 92 3.5 93 2.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14377727409406418 2 0.04971560400352747 3 0.14876778580471053 4 0.12741597620726164 5 0.19235579947997603 6 0.19696728498475052 7 0.16348663679940165 8 0.07081472946372845 9 0.12223595139367936 10-11 0.10255817420549791 12-13 0.14851510166746262 14-15 0.13755492721433427 16-17 0.13439637549873532 18-19 0.1302270872341447 20-21 0.13161684998900824 22-23 0.1921978718941961 24-25 0.21089649805054186 26-27 0.14567240512342358 28-29 0.12447852311175461 30-31 0.0956409459483362 32-33 0.12192009622211945 34-35 0.09049250665190992 36-37 0.08471235701236383 38-39 0.05034731434664726 40-41 0.05906491708170036 42-43 0.060770535008123794 44-45 0.039892508168014734 46-47 0.05281098468481444 48-49 0.043588013675265505 50-51 0.053505866062246214 52-53 0.027384643374242897 54-55 0.051105366758391 56-57 0.026974031651215036 58-59 0.05091585365545507 60-61 0.03733408127837959 62-63 0.052495129513254545 64-65 0.040429461959666554 66-67 0.03957665299645484 68-69 0.06582421775308211 70-71 0.05217927434169465 72-73 0.05138963641279492 74-75 0.04264044816058583 76-77 0.021794006837632753 78-79 0.02331011166112025 80-81 0.02627915027378326 82-83 0.022267789594972595 84-85 0.020341073048457236 86-87 0.024194506141487954 88-89 0.06415018534381467 90-91 0.10814881074210805 92-93 0.07931123357868963 94-95 0.07416279428226334 96-97 0.06601373085601805 98-99 0.05420074743967798 100-101 0.1275107327587296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1583004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.0040382837486 #Duplication Level Percentage of deduplicated Percentage of total 1 79.43353744591371 48.45766559364098 2 12.459816570444767 15.201982541437955 3 3.7345984217942196 6.834767552926851 4 1.529438289189136 3.732076477853001 5 0.7902829630362531 2.410522606602893 6 0.46593115465334656 1.7054209197638364 7 0.3058540154282675 1.3060831046496983 8 0.21074662814922207 1.0285116297428858 9 0.1580262898126425 0.8676217650212275 >10 0.8317379974820084 9.171356727088417 >50 0.05168987389916736 2.1533953828266954 >100 0.025134120668655825 2.9827632916475046 >500 0.0018616816617363803 0.7982716095346793 >1k 0.0010342675898535447 1.4503532633871068 >5k 1.0342675898535446E-4 0.4327651730792259 >10k+ 2.0685351797070892E-4 1.4664423607971087 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13006 0.8216024722615989 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10236 0.646618707217417 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6859 0.43329012434586395 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4738 0.29930436057015647 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4453 0.2813006157912425 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2440 0.15413732372122876 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2345 0.14813607546159074 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2131 0.13461747411882724 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1816 0.11471859831055387 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1586 0.10018926041879869 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.26342068623958E-4 14-15 0.0 0.0 0.0 0.0 1.5792758577994748E-4 16-17 0.0 0.0 0.0 3.15855171559895E-5 1.89513102935937E-4 18-19 0.0 0.0 0.0 1.26342068623958E-4 1.89513102935937E-4 20-21 0.0 0.0 0.0 2.2109862009192649E-4 1.89513102935937E-4 22-23 0.0 0.0 0.0 3.4744068871588453E-4 1.89513102935937E-4 24-25 0.0 0.0 0.0 9.159799975236955E-4 1.89513102935937E-4 26-27 0.0 0.0 0.0 0.0019267165465153594 1.89513102935937E-4 28-29 0.0 0.0 0.0 0.005590636536610141 2.52684137247916E-4 30-31 0.0 0.0 0.0 0.01645605443827053 2.52684137247916E-4 32-33 0.0 0.0 0.0 0.03967140954792281 2.52684137247916E-4 34-35 0.0 6.3171034311979E-5 0.0 0.07150961084116023 2.52684137247916E-4 36-37 0.0 9.47565514679685E-5 0.0 0.12378364173432285 2.52684137247916E-4 38-39 0.0 1.26342068623958E-4 0.0 0.19854656084254999 2.52684137247916E-4 40-41 0.0 1.26342068623958E-4 0.0 0.27662595925215605 2.52684137247916E-4 42-43 0.0 1.89513102935937E-4 0.0 0.3528418121495587 2.52684137247916E-4 44-45 0.0 1.89513102935937E-4 0.0 0.4486722712008308 2.52684137247916E-4 46-47 0.0 1.89513102935937E-4 0.0 0.5602007322786298 2.52684137247916E-4 48-49 0.0 1.89513102935937E-4 0.0 0.6761511657582672 2.52684137247916E-4 50-51 0.0 1.89513102935937E-4 0.0 0.8001874916298379 2.52684137247916E-4 52-53 0.0 1.89513102935937E-4 0.0 0.914305965114428 2.52684137247916E-4 54-55 0.0 1.89513102935937E-4 0.0 1.0376789951257228 2.52684137247916E-4 56-57 0.0 1.89513102935937E-4 0.0 1.1803823616364835 2.52684137247916E-4 58-59 0.0 1.89513102935937E-4 0.0 1.3345828563920243 2.52684137247916E-4 60-61 0.0 1.89513102935937E-4 0.0 1.507513562821067 2.52684137247916E-4 62-63 0.0 1.89513102935937E-4 0.0 1.6739692382331315 2.52684137247916E-4 64-65 0.0 1.89513102935937E-4 0.0 1.8412777226084078 2.52684137247916E-4 66-67 1.26342068623958E-4 1.89513102935937E-4 0.0 2.0244737221131466 2.52684137247916E-4 68-69 1.26342068623958E-4 1.89513102935937E-4 0.0 2.2270948146688196 2.52684137247916E-4 70-71 1.26342068623958E-4 1.89513102935937E-4 0.0 2.4528680912998326 2.52684137247916E-4 72-73 1.26342068623958E-4 1.89513102935937E-4 0.0 2.678199170690662 2.52684137247916E-4 74-75 1.26342068623958E-4 1.89513102935937E-4 0.0 2.896802534927265 2.52684137247916E-4 76-77 1.26342068623958E-4 1.89513102935937E-4 0.0 3.130977559121771 2.52684137247916E-4 78-79 1.26342068623958E-4 1.89513102935937E-4 0.0 3.389441846009233 2.52684137247916E-4 80-81 1.26342068623958E-4 1.89513102935937E-4 0.0 3.6614879052737708 2.52684137247916E-4 82-83 1.26342068623958E-4 1.89513102935937E-4 0.0 3.945757559677676 3.15855171559895E-4 84-85 1.26342068623958E-4 1.89513102935937E-4 0.0 4.229237576152682 3.15855171559895E-4 86-87 1.26342068623958E-4 1.89513102935937E-4 0.0 4.511391000907136 3.15855171559895E-4 88-89 1.26342068623958E-4 1.89513102935937E-4 0.0 4.815875386290875 3.15855171559895E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7585 0.0 34.516743 1 GTATCAA 11055 0.0 30.959423 1 GTACATG 8720 0.0 27.294565 1 TACATGG 8585 0.0 27.196308 2 TATCAAC 13110 0.0 26.0067 2 ATCAACG 13220 0.0 25.682394 3 TCAACGC 13270 0.0 25.585629 4 ACATGGG 8840 0.0 25.55113 3 CAACGCA 13395 0.0 25.346867 5 AACGCAG 13660 0.0 24.883652 6 GAGTACT 8630 0.0 23.709265 12-13 CATGGGA 5385 0.0 23.047485 4 TAGCCCT 1000 0.0 22.824959 4 ATGGGAT 1935 0.0 22.362955 5 ACGCAGA 15215 0.0 22.303606 7 CGCAGAG 15400 0.0 22.03428 8 CATGGGG 3200 0.0 21.844198 4 GTACTTT 9255 0.0 21.746262 14-15 TGACGCG 90 5.169826E-6 21.112833 94-95 GCAGAGT 16110 0.0 20.767525 9 >>END_MODULE