FastQCFastQC Report
Thu 26 May 2016
SRR937924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583004
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125110.7903328102771692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102730.6489560354869602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70420.4448504236249561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48910.30896952881988926No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43410.27422545994830083No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.1614651637014183No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.15179999545168552No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.1371443154913064No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17730.11200224383513875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71650.034.2043721
GTATCAA105750.031.3489591
GTACCGT1503.345849E-828.4934166
GTATACG1056.912381E-527.1400151
TACATGG89700.025.5726282
TATCAAC129300.025.4539622
ATCAACG129650.025.3844433
GTACATG93000.025.2796081
TCAACGC130400.025.2376484
CAACGCA132250.024.9205175
AACGCAG137200.024.0560286
ACATGGG91900.023.9778063
CATGGGA56650.021.7116624
ACGCAGA152300.021.6092827
CGCAGAG152750.021.4523498
GAGTACT87350.021.09748312-13
CATGGGG33750.020.6841094
ACCGTCC1856.565995E-620.5364428
ATGGGAG21800.020.2590775
GCAGAGT160550.020.203079
Produced by FastQC (version 0.11.5)