##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937924_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1583004 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41114235971608 33.0 31.0 34.0 31.0 34.0 2 32.62930668526422 34.0 31.0 34.0 31.0 34.0 3 32.67936846653578 34.0 31.0 34.0 31.0 34.0 4 36.17140765279178 37.0 35.0 37.0 35.0 37.0 5 36.14695793567167 37.0 35.0 37.0 35.0 37.0 6 36.1877120967477 37.0 37.0 37.0 35.0 37.0 7 36.14058018804754 37.0 36.0 37.0 35.0 37.0 8 36.13556188108179 37.0 36.0 37.0 35.0 37.0 9 37.79394935199153 39.0 38.0 39.0 35.0 39.0 10-11 37.76759787088346 39.0 38.0 39.0 35.0 39.0 12-13 37.67368023075115 39.0 37.0 39.0 35.0 39.0 14-15 38.88116517709368 40.0 38.0 41.0 35.5 41.0 16-17 38.75710326695321 40.0 38.0 41.0 35.0 41.0 18-19 39.03744968427117 40.0 38.5 41.0 36.0 41.0 20-21 39.02051416168247 40.0 38.5 41.0 35.0 41.0 22-23 38.89659280709335 40.0 38.0 41.0 35.0 41.0 24-25 38.77674061467943 40.0 38.0 41.0 35.0 41.0 26-27 38.56986305783182 40.0 38.0 41.0 34.0 41.0 28-29 38.43206839654227 40.0 38.0 41.0 34.0 41.0 30-31 38.14903973710743 40.0 37.5 41.0 34.0 41.0 32-33 37.8905072255029 40.0 37.0 41.0 33.0 41.0 34-35 37.70119311132505 40.0 37.0 41.0 33.0 41.0 36-37 37.670063689036795 40.0 37.0 41.0 33.0 41.0 38-39 37.49590114743867 40.0 36.0 41.0 33.0 41.0 40-41 37.292250998734055 40.0 36.0 41.0 32.5 41.0 42-43 37.02298320155856 40.0 36.0 41.0 31.5 41.0 44-45 36.698395897925714 39.0 35.0 41.0 31.0 41.0 46-47 36.239741339882904 39.0 35.0 41.0 30.0 41.0 48-49 35.78645631975661 39.0 35.0 40.0 28.5 41.0 50-51 35.37374005372065 38.5 35.0 40.0 27.0 41.0 52-53 34.81724967214233 38.0 34.0 40.0 26.0 41.0 54-55 34.480864230286215 38.0 34.0 40.0 25.0 41.0 56-57 34.11711530735235 37.5 33.0 40.0 24.0 41.0 58-59 33.656765238748605 37.0 33.0 40.0 22.5 41.0 60-61 33.148614911901674 36.0 32.5 39.0 21.5 41.0 62-63 32.654773140181575 36.0 31.5 39.0 19.5 40.0 64-65 32.13316390861931 35.0 31.0 39.0 17.5 40.0 66-67 31.550117055926577 35.0 30.5 38.0 13.5 40.0 68-69 30.937354232838324 35.0 30.0 38.0 8.0 40.0 70-71 30.290120871457052 34.0 29.0 37.0 7.0 39.0 72-73 29.668784159736802 34.0 28.5 36.0 2.0 39.0 74-75 29.048619270703043 34.0 27.5 36.0 2.0 38.5 76-77 27.731136813299273 31.5 26.0 34.5 2.0 36.5 78-79 28.415397560587337 33.0 27.0 35.0 2.0 37.0 80-81 28.060423094319408 33.0 26.5 35.0 2.0 37.0 82-83 27.44543601911303 33.0 26.0 35.0 2.0 36.0 84-85 26.79852135559986 32.5 25.0 35.0 2.0 36.0 86-87 26.073794823007397 32.0 23.5 34.5 2.0 35.0 88-89 25.250493049922802 31.0 20.0 34.0 2.0 35.0 90-91 24.63352840548729 31.0 18.5 34.0 2.0 35.0 92-93 23.71819812205149 31.0 8.5 34.0 2.0 35.0 94-95 22.789944940126496 30.0 2.0 34.0 2.0 35.0 96-97 21.638896048272777 29.5 2.0 34.0 2.0 35.0 98-99 20.100864558775594 29.0 2.0 34.0 2.0 35.0 100-101 16.00569139433634 13.5 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21938937153578308 1101 2 0.2099785634040714 1101 3 0.18505823539099708 1101 4 0.13670370819566102 1101 5 0.1072761901480277 1101 6 0.1582240431361086 1101 7 0.10939993506902113 1101 8 0.18087488185567224 1101 9 0.10021094125575303 1101 10-11 0.20574491016387952 1101 12-13 0.20085325203327642 1101 14-15 0.21104814983052478 1101 16-17 0.3372945403309444 1101 18-19 0.3719001564056299 1101 20-21 0.3530227421855727 1101 22-23 0.30850456650474456 1101 24-25 0.3534370942534366 1101 26-27 0.26358137906180445 1101 28-29 0.24068346223506865 1101 30-31 0.3969432274856288 1101 32-33 0.2657715693262048 1101 34-35 0.30691878681383855 1101 36-37 0.36578765074113306 1101 38-39 0.37986542942288537 1101 40-41 0.26993316845364745 1101 42-43 0.4109167037886863 1101 44-45 0.4968003697344301 1101 46-47 0.30782021580759533 1101 48-49 0.4121730539919497 1101 50-51 0.20090159505411265 1101 52-53 0.09850849125742656 1101 54-55 0.29487567601938025 1101 56-57 0.24927941435877443 1101 58-59 0.3927468757498005 1101 60-61 0.29375351828807794 1101 62-63 0.4053391765140617 1101 64-65 0.44310487145298083 1101 66-67 -0.015064492270948193 1101 68-69 0.26179636892022984 1101 70-71 0.1269466029814943 1101 72-73 0.3484500995147002 1101 74-75 0.23667993223550354 1101 76-77 0.27410399629131277 1101 78-79 0.21571242546388092 1101 80-81 0.24849955994509187 1101 82-83 0.2094336913385213 1101 84-85 0.14551857013776726 1101 86-87 -0.0275167429527734 1101 88-89 -0.17860941674551967 1101 90-91 -0.24316906729756127 1101 92-93 -0.43595040833579546 1101 94-95 -0.5843783483865828 1101 96-97 -0.7932903782046203 1101 98-99 -0.5407513986210439 1101 100-101 -0.9248350678869102 1102 1 0.10694388008171529 1102 2 0.015835412684808148 1102 3 0.10684160464567327 1102 4 0.13472886984169463 1102 5 0.11289244998462067 1102 6 0.07903001777397378 1102 7 0.011547697085966036 1102 8 0.03870656949037965 1102 9 0.06526532449468903 1102 10-11 0.15023892048917986 1102 12-13 0.08406086067444818 1102 14-15 0.10287437407993849 1102 16-17 0.05550290081413323 1102 18-19 0.13359004663669793 1102 20-21 0.1696772958420283 1102 22-23 0.1322452311330906 1102 24-25 0.11533624717809943 1102 26-27 0.02328891251877252 1102 28-29 -0.08934636083144198 1102 30-31 -0.052961539192736495 1102 32-33 -0.16499631198300335 1102 34-35 0.02523867052893536 1102 36-37 0.04197305498151849 1102 38-39 0.08532756164682809 1102 40-41 0.008369838294768783 1102 42-43 -0.008698854328699213 1102 44-45 -0.25723827494503126 1102 46-47 -0.04935987541590947 1102 48-49 -0.08834446392695128 1102 50-51 -0.09055747967898498 1102 52-53 -0.10454148117504047 1102 54-55 -0.12166234212507732 1102 56-57 -0.01175136104095742 1102 58-59 -0.06843301498249588 1102 60-61 -0.14377091657734553 1102 62-63 -0.3253121692831442 1102 64-65 -0.16277954536823813 1102 66-67 -0.47280595886410026 1102 68-69 -0.30918503552065246 1102 70-71 -0.489540039292077 1102 72-73 -0.2595858385335177 1102 74-75 -0.35642692957593525 1102 76-77 -0.29424470803428804 1102 78-79 -0.33487401130620853 1102 80-81 -0.4655072567558243 1102 82-83 -0.2344375431860648 1102 84-85 -0.5641488650785149 1102 86-87 -0.6609035406070269 1102 88-89 -0.6821380619262136 1102 90-91 -0.9109325879230958 1102 92-93 -0.697743050308631 1102 94-95 -0.8029400746158437 1102 96-97 -0.8634798423918788 1102 98-99 -1.1227147087308147 1102 100-101 -1.309817023678601 1103 1 0.05742861940515098 1103 2 0.04087699692118463 1103 3 -0.0061720467947665725 1103 4 0.014182812224149188 1103 5 0.06676150639155054 1103 6 0.01814282178180804 1103 7 0.043818540876870316 1103 8 -0.06123339173047526 1103 9 0.044576551746388304 1103 10-11 0.008200948421972498 1103 12-13 0.07532448012700854 1103 14-15 0.0815687274819723 1103 16-17 0.09176706159995263 1103 18-19 0.07309236452726964 1103 20-21 0.09191797260702117 1103 22-23 0.12737273972060592 1103 24-25 0.11364285833350607 1103 26-27 0.12204409209377332 1103 28-29 0.06732212668345028 1103 30-31 0.13454006020097609 1103 32-33 0.12938907792457854 1103 34-35 0.012829343849084296 1103 36-37 0.1037920823533014 1103 38-39 0.16046929253531061 1103 40-41 0.17597005876503147 1103 42-43 0.2551651824425889 1103 44-45 0.1553558432230986 1103 46-47 -0.08880633755703116 1103 48-49 -0.1811932055731944 1103 50-51 -0.2217943769536177 1103 52-53 -0.13697275645775875 1103 54-55 -0.060660481907163444 1103 56-57 -0.25233979028870124 1103 58-59 -0.11075937003805336 1103 60-61 -0.054087252046478795 1103 62-63 -0.14097564700481513 1103 64-65 -0.500885469522526 1103 66-67 -0.6469449929287237 1103 68-69 -0.34911131577379706 1103 70-71 -0.6565002562190756 1103 72-73 -0.777484530475526 1103 74-75 -0.6436836987022225 1103 76-77 -0.5032980145962291 1103 78-79 -0.6155548116920322 1103 80-81 -0.5764308697123894 1103 82-83 -0.602829340510894 1103 84-85 -0.46246753320185974 1103 86-87 -0.6253103869963361 1103 88-89 -0.7950444309580185 1103 90-91 -1.0134615984643283 1103 92-93 -0.73729605667668 1103 94-95 -0.7774740358957537 1103 96-97 -1.0705973904475101 1103 98-99 -1.3432711008923057 1103 100-101 -1.2382771274177369 1104 1 0.16823655216526134 1104 2 0.17302333538555104 1104 3 0.1527018568236116 1104 4 0.10990457146581178 1104 5 0.05520130648156396 1104 6 0.09136649466081082 1104 7 0.07372431623673492 1104 8 0.07387527142664396 1104 9 0.16919177101802063 1104 10-11 0.2554536879387328 1104 12-13 0.22218764015590153 1104 14-15 0.31245992634584496 1104 16-17 0.29853892082945777 1104 18-19 0.23130781733070194 1104 20-21 0.16043510664236038 1104 22-23 0.2555064235393658 1104 24-25 0.3163128928141745 1104 26-27 0.26881597009593605 1104 28-29 0.14914742931034652 1104 30-31 0.13949345943569824 1104 32-33 0.23919924416112082 1104 34-35 0.2463275949417678 1104 36-37 0.1867589969597887 1104 38-39 0.2561816654503133 1104 40-41 0.2763457934210862 1104 42-43 0.2847361005989839 1104 44-45 0.135406613863978 1104 46-47 0.21769524239640248 1104 48-49 0.2539357789811376 1104 50-51 0.3401342597434507 1104 52-53 0.3144565229680225 1104 54-55 0.34752153210755665 1104 56-57 0.20346562928244794 1104 58-59 0.1789104142970075 1104 60-61 -0.09039309423698683 1104 62-63 0.030765971826781424 1104 64-65 0.07937560176709013 1104 66-67 -0.03491683770328535 1104 68-69 0.030470560918967493 1104 70-71 -0.10032121391537174 1104 72-73 -0.27599703235865647 1104 74-75 -0.281510642059029 1104 76-77 -0.18298727086594013 1104 78-79 -0.04356450213566987 1104 80-81 -0.28365773112656356 1104 82-83 -0.24567984589418757 1104 84-85 -0.30375976756529255 1104 86-87 -0.269888680458898 1104 88-89 -0.3904695822773334 1104 90-91 -0.4116415427940261 1104 92-93 -0.46655825854934463 1104 94-95 -0.8545615901428576 1104 96-97 -1.3035298807009816 1104 98-99 -1.5459711716182802 1104 100-101 -1.9792853784681839 1105 1 0.20520928102554592 1105 2 0.17943343028588998 1105 3 0.19163288792100275 1105 4 0.188993396197759 1105 5 0.1895309354901542 1105 6 0.16645053050104508 1105 7 0.2005178699992527 1105 8 0.1887257010976171 1105 9 0.1862044131013576 1105 10-11 0.2760593020280808 1105 12-13 0.2597505095639079 1105 14-15 0.22133045337589152 1105 16-17 0.1432746151883464 1105 18-19 0.21461468085858826 1105 20-21 0.1909949169633265 1105 22-23 0.28074932165292665 1105 24-25 0.14530636542965425 1105 26-27 0.2739152448985607 1105 28-29 0.22914524859224628 1105 30-31 0.20927904532341302 1105 32-33 0.3719878178932774 1105 34-35 0.3625033973113716 1105 36-37 0.22983860533408773 1105 38-39 0.20199255809382066 1105 40-41 0.34183010832373384 1105 42-43 0.09401601184114838 1105 44-45 0.04052104943592383 1105 46-47 0.042464551091384806 1105 48-49 0.13635945167532526 1105 50-51 0.12140888513344095 1105 52-53 0.12114042396863312 1105 54-55 -0.014729412073876347 1105 56-57 -0.037744382559459666 1105 58-59 0.05851066520990855 1105 60-61 -0.0832038921799878 1105 62-63 -0.14358330970913613 1105 64-65 -0.06546252552602283 1105 66-67 -0.36101086748790223 1105 68-69 -0.39665109969352486 1105 70-71 -0.161386968213602 1105 72-73 -0.4011383229093717 1105 74-75 -0.5855001398596293 1105 76-77 -0.308184816993343 1105 78-79 -0.6555215706597401 1105 80-81 -0.7534169920108233 1105 82-83 -0.31307163753222156 1105 84-85 -0.5756592599184458 1105 86-87 -0.8439766233466734 1105 88-89 -0.8365656624582556 1105 90-91 -0.7939500978988612 1105 92-93 -0.7251536328205184 1105 94-95 -0.7639138734242188 1105 96-97 -1.1537618969442924 1105 98-99 -1.2671720088055167 1105 100-101 -2.097887555537284 1106 1 0.14242442504302488 1106 2 0.14196782907369254 1106 3 0.17315891147170248 1106 4 0.17604943001867923 1106 5 0.20852945610092632 1106 6 0.11293093571945434 1106 7 0.21137101510352352 1106 8 0.24579987559566518 1106 9 0.12308487863545281 1106 10-11 0.23348065948034247 1106 12-13 0.2020761664393831 1106 14-15 0.20805295802139057 1106 16-17 0.3595267954255803 1106 18-19 0.3688060691398718 1106 20-21 0.33825367951721574 1106 22-23 0.3636300575042455 1106 24-25 0.39872463721984985 1106 26-27 0.37715964937773805 1106 28-29 0.3306960328340338 1106 30-31 0.33111856331284173 1106 32-33 0.41441498164943624 1106 34-35 0.42165598035806084 1106 36-37 0.4257671128611449 1106 38-39 0.49303825365960563 1106 40-41 0.3803174443289805 1106 42-43 0.39960619987712676 1106 44-45 0.39628059949563976 1106 46-47 0.469043079627788 1106 48-49 0.4040484507751927 1106 50-51 0.5237993078121335 1106 52-53 0.4227130910343391 1106 54-55 0.29693831655335856 1106 56-57 0.2583375805688988 1106 58-59 0.5852723014934753 1106 60-61 0.3030608201114475 1106 62-63 0.4350927978329864 1106 64-65 0.40068782602430275 1106 66-67 0.41128065034338235 1106 68-69 0.4217543320279269 1106 70-71 0.03145633697547723 1106 72-73 0.28906502185268224 1106 74-75 0.4491450595728885 1106 76-77 0.36004996547943335 1106 78-79 0.5534444567158552 1106 80-81 0.46161117522911965 1106 82-83 0.5108743780928542 1106 84-85 0.23189831351543688 1106 86-87 -0.0649637225944737 1106 88-89 -0.15458768503802034 1106 90-91 -0.19436302126944227 1106 92-93 -0.26262612988059075 1106 94-95 -0.6131258159293971 1106 96-97 -0.7895632082587234 1106 98-99 -1.1350549191475423 1106 100-101 -1.81122922824931 1107 1 -0.06673543555032779 1107 2 -0.03949927240370954 1107 3 -0.07576516027460656 1107 4 0.004997237932450105 1107 5 -0.01233021258209277 1107 6 0.015819822635265268 1107 7 0.06497205046381538 1107 8 -0.036123134491994335 1107 9 -0.016183511292190644 1107 10-11 0.03551260902131759 1107 12-13 -0.029694993905742706 1107 14-15 -0.02426678937910509 1107 16-17 -0.1298392831642161 1107 18-19 -0.06758024100228965 1107 20-21 -0.07181355886383756 1107 22-23 -0.0847160846497701 1107 24-25 -0.18296917129346468 1107 26-27 -0.09124406164985288 1107 28-29 -0.10204064072854635 1107 30-31 -0.11311729825634131 1107 32-33 -0.13072052041024307 1107 34-35 0.015462464213115368 1107 36-37 -0.14463933201146517 1107 38-39 -0.05123832385056204 1107 40-41 -0.1608382119100895 1107 42-43 -0.11651655170869901 1107 44-45 -0.3598455006909447 1107 46-47 -0.35339419435752006 1107 48-49 -0.12145080572550881 1107 50-51 -0.3504028098396006 1107 52-53 -0.19208620220795325 1107 54-55 -0.22208169345015705 1107 56-57 -0.15774741469781617 1107 58-59 -0.19238589346696955 1107 60-61 -0.3051985543851501 1107 62-63 -0.1623933416235488 1107 64-65 -0.5717363199239571 1107 66-67 -0.6291448248978995 1107 68-69 -0.6136361921929883 1107 70-71 -0.6472729028625572 1107 72-73 -0.7637230053775319 1107 74-75 -0.6955009633083691 1107 76-77 -0.8140667036606324 1107 78-79 -0.8184472532564193 1107 80-81 -0.7883654648981953 1107 82-83 -0.8833489473636611 1107 84-85 -1.034130892878867 1107 86-87 -1.1666781047078736 1107 88-89 -1.2590951045986678 1107 90-91 -1.6271817186383792 1107 92-93 -1.3805180165845705 1107 94-95 -1.5057232519666854 1107 96-97 -1.3606968745072372 1107 98-99 -1.4283405518322532 1107 100-101 -1.8279335455343038 1108 1 -0.06727740919238556 1108 2 -0.0658614995578759 1108 3 -0.01664498798704983 1108 4 -0.011105027774775067 1108 5 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-0.6097456959282681 2316 44-45 -0.4213963327578014 2316 46-47 -0.47813517129483074 2316 48-49 -0.44626690603598007 2316 50-51 -0.2395791613289049 2316 52-53 -7.105101559048421E-4 2316 54-55 -0.09131212873474226 2316 56-57 0.016860633506972533 2316 58-59 -0.06328930236304586 2316 60-61 0.33906065774431227 2316 62-63 0.2116812614227186 2316 64-65 0.5972929998980732 2316 66-67 0.3975905077196451 2316 68-69 0.5916681534173129 2316 70-71 0.6781272281943842 2316 72-73 0.7763626126176 2316 74-75 1.082020758136803 2316 76-77 0.773846360853927 2316 78-79 0.8058336402083803 2316 80-81 0.8476927789954338 2316 82-83 1.1429991286694978 2316 84-85 1.3024834778952439 2316 86-87 1.6696488908511036 2316 88-89 1.9266545137286677 2316 90-91 1.8043380416714996 2316 92-93 1.7667170311744513 2316 94-95 2.3168443378345316 2316 96-97 2.730148612632288 2316 98-99 3.212570668258781 2316 100-101 3.3490677231950237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 13.0 5 43.0 6 102.0 7 215.0 8 511.0 9 863.0 10 1520.0 11 2521.0 12 3902.0 13 6037.0 14 7979.0 15 10834.0 16 14064.0 17 19090.0 18 19727.0 19 15107.0 20 14065.0 21 15303.0 22 16640.0 23 18147.0 24 20296.0 25 23213.0 26 26127.0 27 31137.0 28 37282.0 29 44699.0 30 53528.0 31 64397.0 32 79306.0 33 101545.0 34 136037.0 35 190993.0 36 233597.0 37 254209.0 38 115753.0 39 4198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55588288623529 18.975737868144353 14.326364622510088 22.142014623110278 2 15.871318042995073 20.668365780339222 39.928328908645895 23.531987268019808 3 19.677342727323268 25.437816200589314 29.625373375720848 25.259467696366567 4 12.860927704541492 16.82888988277983 36.50565633441229 33.804526078266385 5 13.148307598230433 38.232720799349586 33.80571034379941 14.81326125862057 6 31.613605452490646 35.954334316919386 17.44415448555038 14.987905745039587 7 28.236315258836996 33.659232699348834 20.255855323170376 17.848596718643794 8 26.595953010857837 34.83415076651733 19.443602163986952 19.12629405863788 9 26.88521317697239 16.65106342119161 20.264509754871117 36.199213646964886 10-11 25.10489550247504 26.969672849847505 26.505650017308863 21.41978163036859 12-13 26.202823953478017 24.525400767596327 27.41483145698324 21.856943821942416 14-15 22.920914918724147 25.56831821018772 26.582086968826356 24.928679902261774 16-17 21.478755457735968 28.88671504229893 27.374894935720086 22.259634564245008 18-19 21.42347713587584 26.968630527781357 30.3723490275451 21.235543308797702 20-21 22.25256537570341 26.03891083029481 30.653333788164783 21.055190005837005 22-23 21.89672293942403 26.149775995512332 30.489310197573726 21.46419086748991 24-25 21.510105866165656 26.394176160592114 30.733217009846225 21.362500963396005 26-27 21.407693838673907 26.523935439234975 30.626810598378086 21.44156012371303 28-29 21.25824473822946 26.837896308903225 30.637970034180857 21.26588891868645 30-31 21.347741434989818 26.68062581809177 30.53484881411439 21.436783932804026 32-33 21.028531829357348 26.696710810585444 30.509809198207964 21.764948161849244 34-35 21.625704179304567 26.761087921883714 30.3527577421028 21.260450156708917 36-37 21.791985748511127 26.467560648581724 30.282676639339734 21.457776963567422 38-39 21.508489470050115 26.624787344729278 30.37064716060598 21.49607602461463 40-41 21.65642013071598 26.616798507113828 30.347118777693126 21.379662584477067 42-43 21.380020714429886 26.734975642066782 30.11820617712006 21.766797466383274 44-45 21.503546421866275 27.017651250407454 29.954251536951265 21.524550790775006 46-47 21.79310462456483 27.12922843481661 29.300986795350358 21.7766801452682 48-49 21.769977359329676 27.158191576457746 29.269667818915067 21.802163245297514 50-51 21.859382195916556 27.451578687357426 29.10642233479834 21.58261678192768 52-53 21.908198421906683 27.458975354413955 28.974270931816754 21.65855529186261 54-55 21.60412361494817 27.513341162928658 29.05233678041902 21.83019844170416 56-57 21.784049898403776 27.67653607424106 28.718244862061642 21.821169165293515 58-59 21.74009608051403 27.327943152889084 28.887719247253468 22.044241519343423 60-61 21.581304907736524 27.452849587744826 29.072968360445866 21.892877144072784 62-63 21.671732436374597 27.41009482956615 29.10971032390282 21.808462410156434 64-65 21.488075361328036 27.440300985329525 29.095448604682534 21.976175048659904 66-67 21.509856310964224 27.567470920076094 28.847423510212643 22.075249258747036 68-69 21.729974603523658 27.351006601567263 28.915395421051333 22.003623373857742 70-71 21.58626811731554 27.418116683725692 28.605329997346374 22.390285201612397 72-73 21.590022496384847 27.504310069801335 28.522712125241878 22.382955308571937 74-75 21.56155731151127 27.768544496081322 28.249766781098984 22.420131411308425 76-77 21.5269421256799 27.448956026788746 28.70991884332867 22.314183004202683 78-79 21.528811373468457 27.402801284345873 28.759275506596598 22.309111835589068 80-81 21.542015082588435 27.61551624522963 28.516883015961714 22.325585656220216 82-83 21.558481197918788 27.62131626101263 28.43998636287086 22.380216178197717 84-85 21.62032548295958 27.51181789432814 28.338904637656707 22.528951985055574 86-87 21.61794972414059 27.480064884307136 28.361608054045824 22.540377337506452 88-89 21.59631354574801 27.47458549188423 28.189395188729854 22.73970577363791 90-91 21.519280086264285 27.44884376983511 28.395463479378748 22.636412664521856 92-93 21.44104166113788 27.37981051745643 28.370095019290726 22.809052802114966 94-95 21.315988657873657 27.32007515927752 28.37272203450655 22.991214148342277 96-97 21.410143049978565 27.06365460267879 28.265820301579165 23.260382045763485 98-99 21.34089365533145 27.10596559948194 28.07954132642585 23.47359941876076 100-101 21.89979224072657 26.55629132796935 28.04753350224896 23.49638292905512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4750.0 1 3039.5 2 2974.5 3 3836.0 4 2307.0 5 1786.0 6 2926.0 7 5244.0 8 7436.0 9 7760.5 10 5549.0 11 3360.0 12 2953.0 13 2994.5 14 2953.5 15 2901.5 16 2933.5 17 3059.0 18 3211.0 19 3437.0 20 3740.0 21 4230.0 22 4861.5 23 5750.0 24 6954.5 25 8371.5 26 10286.5 27 12668.5 28 14658.0 29 16506.0 30 18902.0 31 21530.5 32 24822.5 33 27048.0 34 28597.0 35 30909.0 36 33985.5 37 37194.0 38 39150.5 39 40409.0 40 41970.0 41 44035.5 42 45035.5 43 46107.0 44 48115.0 45 50885.5 46 53103.0 47 55127.0 48 60336.5 49 61337.5 50 57644.0 51 55040.0 52 53125.5 53 53012.5 54 52781.5 55 49654.5 56 45099.5 57 41044.5 58 37750.5 59 33881.5 60 28552.0 61 23952.0 62 20031.0 63 15392.0 64 11056.0 65 8294.5 66 6389.0 67 5033.5 68 3882.0 69 2900.0 70 2246.0 71 1656.0 72 1257.5 73 1067.0 74 842.0 75 583.5 76 427.5 77 291.0 78 178.0 79 116.5 80 71.5 81 37.0 82 20.0 83 9.5 84 6.5 85 5.5 86 3.5 87 3.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010549562730100492 2 0.013771285480011422 3 0.009349313078172891 4 0.0 5 0.0010739075833036428 6 0.001326591720551559 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.632958602757795E-4 14-15 0.0 16-17 0.0028111110268830655 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003916604127342698 26-27 0.01951984960240151 28-29 0.006348688948353889 30-31 0.0032848937842229076 32-33 0.0 34-35 0.008401747563493206 36-37 7.264668945877585E-4 38-39 0.002716354475415097 40-41 0.0018951310293593697 42-43 0.00312696619844296 44-45 0.0 46-47 6.3171034311979E-5 48-49 7.58052411743748E-4 50-51 0.004737827573398425 52-53 0.009128214458080966 54-55 0.007643695151749458 56-57 0.01692983719561037 58-59 0.023562795798368168 60-61 0.004074531713122645 62-63 0.020372658565613225 64-65 0.021193882011668955 66-67 0.01332908823982757 68-69 0.03164868819030148 70-71 0.016677153058362454 72-73 0.005243195847894257 74-75 0.016645567541206465 76-77 0.020499000634237183 78-79 0.02580536751644342 80-81 0.011718226864872104 82-83 0.03534419369755225 84-85 0.02150973718322885 86-87 0.0312696619844296 88-89 0.021794006837632753 90-91 0.026879275099747066 92-93 0.023878650969928063 94-95 0.014655679960379127 96-97 0.014876778580471053 98-99 0.01130761514184424 100-101 0.010265293075696586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1583004.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.867673814660215 #Duplication Level Percentage of deduplicated Percentage of total 1 78.41284847883088 46.94394835609024 2 13.134785072138094 15.726980566488635 3 3.8787537571057107 6.966358942133775 4 1.5859935052447278 3.7979896737664385 5 0.8229691657772948 2.4634624788139057 6 0.49488979551739276 1.77767405113435 7 0.3076550701417808 1.2893015370672178 8 0.22503983749550902 1.0778089269188222 9 0.16506968573363665 0.8894104291970848 >10 0.8851213764272743 9.484174609873019 >50 0.055638232820845415 2.2481596935377124 >100 0.02797589047386287 3.2059285013379615 >500 0.0016826489254089237 0.6709036048930601 >1k 0.0012619866940566929 1.5800485049170638 >5k 1.0516555783805773E-4 0.44336553919453164 >10k+ 2.1033111567611546E-4 1.4344845846362124 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12511 0.7903328102771692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10273 0.6489560354869602 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7042 0.4448504236249561 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4891 0.30896952881988926 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4341 0.27422545994830083 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2556 0.1614651637014183 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2403 0.15179999545168552 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2171 0.1371443154913064 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1773 0.11200224383513875 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.26342068623958E-4 2 0.0 0.0 0.0 0.0 1.26342068623958E-4 3 0.0 0.0 0.0 0.0 1.26342068623958E-4 4 0.0 0.0 0.0 0.0 1.26342068623958E-4 5 0.0 0.0 0.0 0.0 1.89513102935937E-4 6 1.26342068623958E-4 0.0 0.0 0.0 1.89513102935937E-4 7 1.26342068623958E-4 0.0 0.0 6.3171034311979E-5 1.89513102935937E-4 8 1.26342068623958E-4 0.0 0.0 6.3171034311979E-5 1.89513102935937E-4 9 1.26342068623958E-4 0.0 0.0 6.3171034311979E-5 1.89513102935937E-4 10-11 1.26342068623958E-4 0.0 0.0 6.3171034311979E-5 1.89513102935937E-4 12-13 1.26342068623958E-4 0.0 0.0 6.3171034311979E-5 3.15855171559895E-4 14-15 1.26342068623958E-4 0.0 0.0 1.26342068623958E-4 3.15855171559895E-4 16-17 1.26342068623958E-4 0.0 0.0 1.26342068623958E-4 3.4744068871588453E-4 18-19 1.26342068623958E-4 0.0 0.0 1.89513102935937E-4 3.79026205871874E-4 20-21 1.26342068623958E-4 0.0 0.0 2.842696544039055E-4 3.79026205871874E-4 22-23 1.26342068623958E-4 0.0 0.0 4.1061172302786345E-4 3.79026205871874E-4 24-25 1.26342068623958E-4 0.0 0.0 0.0010107365489916641 3.79026205871874E-4 26-27 1.26342068623958E-4 0.0 0.0 0.002053058615139317 3.79026205871874E-4 28-29 1.26342068623958E-4 0.0 0.0 0.005843320673858057 4.4219724018385297E-4 30-31 1.26342068623958E-4 0.0 0.0 0.016645567541206465 4.4219724018385297E-4 32-33 1.26342068623958E-4 0.0 0.0 0.040082021270950674 4.4219724018385297E-4 34-35 1.26342068623958E-4 0.0 0.0 0.07195180808134408 4.4219724018385297E-4 36-37 1.26342068623958E-4 3.15855171559895E-5 0.0 0.12435218104313064 4.4219724018385297E-4 38-39 1.26342068623958E-4 6.3171034311979E-5 0.0 0.19962046842585363 4.4219724018385297E-4 40-41 1.26342068623958E-4 6.3171034311979E-5 0.0 0.2778262089040836 4.4219724018385297E-4 42-43 1.26342068623958E-4 1.26342068623958E-4 0.0 0.35413681835295424 4.4219724018385297E-4 44-45 1.26342068623958E-4 1.26342068623958E-4 0.0 0.45015679050716234 4.4219724018385297E-4 46-47 1.26342068623958E-4 1.26342068623958E-4 0.0 0.5617484226192733 4.737827573398425E-4 48-49 1.26342068623958E-4 1.26342068623958E-4 0.0 0.6770355602386349 5.05368274495832E-4 50-51 1.26342068623958E-4 1.26342068623958E-4 0.0 0.8007560309386458 5.05368274495832E-4 52-53 1.26342068623958E-4 1.26342068623958E-4 0.0 0.9153482871805756 5.05368274495832E-4 54-55 1.26342068623958E-4 1.26342068623958E-4 0.0 1.0381843634002188 5.05368274495832E-4 56-57 1.26342068623958E-4 1.26342068623958E-4 0.0 1.1796243092247398 5.05368274495832E-4 58-59 1.26342068623958E-4 1.26342068623958E-4 0.0 1.3336037053601886 5.68539308807811E-4 60-61 1.26342068623958E-4 1.26342068623958E-4 0.0 1.506376484203451 5.68539308807811E-4 62-63 1.89513102935937E-4 1.26342068623958E-4 0.0 1.67201093616946 5.68539308807811E-4 64-65 1.89513102935937E-4 1.26342068623958E-4 0.0 1.8389719798560207 5.68539308807811E-4 66-67 1.89513102935937E-4 1.26342068623958E-4 0.0 2.0212204138460796 5.68539308807811E-4 68-69 1.89513102935937E-4 1.26342068623958E-4 0.0 2.2237151643331288 5.68539308807811E-4 70-71 1.89513102935937E-4 1.26342068623958E-4 0.0 2.447877579589186 6.001248259638004E-4 72-73 1.89513102935937E-4 1.26342068623958E-4 0.0 2.6726401196712075 6.632958602757795E-4 74-75 1.89513102935937E-4 1.26342068623958E-4 0.0 2.890043234255883 6.94881377431769E-4 76-77 1.89513102935937E-4 1.26342068623958E-4 0.0 3.122575811558278 6.94881377431769E-4 78-79 1.89513102935937E-4 1.26342068623958E-4 0.0 3.3810085129285836 7.58052411743748E-4 80-81 1.89513102935937E-4 1.26342068623958E-4 0.0 3.654002137707801 8.212234460557269E-4 82-83 1.89513102935937E-4 1.26342068623958E-4 0.0 3.939914239003818 8.843944803677059E-4 84-85 1.89513102935937E-4 1.26342068623958E-4 0.0 4.223141571341576 8.843944803677059E-4 86-87 1.89513102935937E-4 1.26342068623958E-4 0.0 4.503368279549514 8.843944803677059E-4 88-89 1.89513102935937E-4 1.26342068623958E-4 0.0 4.80630497459261 8.843944803677059E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7165 0.0 34.204372 1 GTATCAA 10575 0.0 31.348959 1 GTACCGT 150 3.345849E-8 28.493416 6 GTATACG 105 6.912381E-5 27.140015 1 TACATGG 8970 0.0 25.572628 2 TATCAAC 12930 0.0 25.453962 2 ATCAACG 12965 0.0 25.384443 3 GTACATG 9300 0.0 25.279608 1 TCAACGC 13040 0.0 25.237648 4 CAACGCA 13225 0.0 24.920517 5 AACGCAG 13720 0.0 24.056028 6 ACATGGG 9190 0.0 23.977806 3 CATGGGA 5665 0.0 21.711662 4 ACGCAGA 15230 0.0 21.609282 7 CGCAGAG 15275 0.0 21.452349 8 GAGTACT 8735 0.0 21.097483 12-13 CATGGGG 3375 0.0 20.684109 4 ACCGTCC 185 6.565995E-6 20.536442 8 ATGGGAG 2180 0.0 20.259077 5 GCAGAGT 16055 0.0 20.20307 9 >>END_MODULE