FastQCFastQC Report
Thu 26 May 2016
SRR937923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413314
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77810.5505499839384596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64120.45368545135758936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40600.2872680805539321No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20870.14766711431429957No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20780.14703031315051007No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20660.14618124493212406No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17240.1219828007081229No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.11504874359130385No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA15990.11313834009993531No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC15260.1079731751047538No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14570.10309103284903424No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14420.10202969757605175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35650.048.9227261
GTATCAA60250.040.4950521
TATCAAC76800.031.5559792
ATCAACG77000.031.2271653
TCAACGC79100.030.3981254
CAACGCA79150.030.2588465
AACGCAG82350.029.021226
CGCAGAG90450.026.2075468
ACGCAGA90900.026.1300667
TACATGG48100.024.1055762
GTACATG49800.023.9221251
GACCGGT1000.001652929523.752237
GCAGAGT98850.023.7385359
GAGTACT62350.022.89266212-13
ACATGGG48600.022.6842523
AGAGTAC85000.022.32551610-11
AGTACTT64100.022.00830812-13
TAAGGTG8500.021.8031085
GTACTTT65250.021.73077214-15
TGGTATC17250.021.4871222
Produced by FastQC (version 0.11.5)