FastQCFastQC Report
Thu 26 May 2016
SRR937923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413314
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.548781091816822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69090.4888510267357431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.3055938029340967No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19100.135143358093106No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18630.13181784090442747No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18050.12771401118222844No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17850.12629889748491843No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17520.12396395988435689No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16670.11794972667078937No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG15180.10740712962582978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.046.747621
GTATCAA67250.038.7451931
TATCAAC84400.030.70322
ATCAACG85400.030.2869283
TCAACGC86950.029.7470234
CAACGCA88250.029.3077855
AACGCAG90400.028.6339366
GGACCGA5250.027.140326
ACGCAGA103150.024.9564657
CGCAGAG102950.024.8665438
ACCGAGT5400.024.6273298
TGGTATC20550.024.294452
AGGACCG6650.023.5934315
GTGTAGG6100.023.3841171
GTGTAAG6150.023.1940041
GTGGTAT22600.022.93231
GAGTACT69950.022.91601612-13
GCAGAGT110550.022.8562989
TAAGGTG9250.022.1016925
GTACTTT72000.022.03266314-15
Produced by FastQC (version 0.11.5)