##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937923_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1413314 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875903726984944 31.0 31.0 34.0 30.0 34.0 2 32.17572174336347 33.0 31.0 34.0 30.0 34.0 3 32.23466052129958 34.0 31.0 34.0 30.0 34.0 4 35.875282492071825 37.0 35.0 37.0 35.0 37.0 5 35.76865013719527 37.0 35.0 37.0 35.0 37.0 6 35.83488948669581 37.0 35.0 37.0 35.0 37.0 7 35.78726312765599 37.0 35.0 37.0 33.0 37.0 8 35.77361860138653 37.0 35.0 37.0 33.0 37.0 9 37.33832113741178 39.0 37.0 39.0 34.0 39.0 10-11 37.26044813820566 39.0 37.0 39.0 34.0 39.0 12-13 37.13608759270764 39.0 37.0 39.0 33.0 39.0 14-15 38.09201175393437 40.0 37.5 41.0 33.0 41.0 16-17 37.91051776179957 40.0 37.0 41.0 33.0 41.0 18-19 38.24381100024482 40.0 38.0 41.0 33.5 41.0 20-21 38.1892916223854 40.0 38.0 41.0 33.0 41.0 22-23 38.01140015594553 40.0 37.0 41.0 33.0 41.0 24-25 37.805923524425566 40.0 37.0 41.0 32.5 41.0 26-27 37.522804911010574 39.0 37.0 41.0 32.0 41.0 28-29 37.32643665880335 39.0 36.0 40.5 31.5 41.0 30-31 36.94216430319094 39.0 36.0 40.0 31.0 41.0 32-33 36.58074391111954 38.0 35.0 40.0 30.0 41.0 34-35 36.374755715998006 38.0 35.0 40.0 30.0 41.0 36-37 36.21777467710643 38.0 35.0 40.0 30.0 41.0 38-39 35.890981055872935 38.0 35.0 40.0 29.5 41.0 40-41 35.51806746413041 38.0 34.5 40.0 27.5 41.0 42-43 35.08451837312869 38.0 34.0 40.0 26.5 41.0 44-45 34.6338722322145 38.0 33.0 40.0 25.5 41.0 46-47 34.03949122417241 37.5 33.0 40.0 23.5 41.0 48-49 33.363729150068565 37.0 32.0 40.0 22.0 41.0 50-51 32.72047259137035 36.5 31.0 39.0 18.5 40.0 52-53 31.86258220041689 36.0 30.0 39.0 13.5 40.0 54-55 31.15395977114781 35.0 29.5 38.5 8.5 40.0 56-57 30.477336246580734 35.0 28.0 38.0 6.0 40.0 58-59 29.665207094814036 34.0 27.5 38.0 2.0 40.0 60-61 28.84730498671916 34.0 26.0 38.0 2.0 40.0 62-63 27.943432245063732 33.0 24.5 37.0 2.0 39.0 64-65 27.041758236315495 32.5 22.5 36.0 2.0 39.0 66-67 26.158196975335983 31.5 20.0 36.0 2.0 39.0 68-69 25.216919948433258 31.0 16.5 35.0 2.0 38.0 70-71 24.309147507206468 30.0 10.0 35.0 2.0 38.0 72-73 23.379728071751924 29.5 7.0 34.0 2.0 37.0 74-75 22.535927614104153 29.0 3.5 34.0 2.0 36.0 76-77 21.64106171735368 27.5 2.0 32.5 2.0 35.0 78-79 22.110546559363314 29.0 2.0 34.0 2.0 35.5 80-81 21.659411850445125 29.0 2.0 34.0 2.0 35.0 82-83 20.89118660113747 28.0 2.0 34.0 2.0 35.0 84-85 19.991989748916374 26.5 2.0 33.0 2.0 35.0 86-87 19.001374782956937 25.5 2.0 33.0 2.0 35.0 88-89 17.930232064495222 23.0 2.0 32.0 2.0 34.0 90-91 17.01151548771186 19.0 2.0 32.0 2.0 34.0 92-93 15.85765901986395 9.5 2.0 31.0 2.0 34.0 94-95 14.732494336007427 2.0 2.0 31.0 2.0 34.0 96-97 13.543012380829737 2.0 2.0 30.0 2.0 34.0 98-99 12.149139540116352 2.0 2.0 29.0 2.0 34.0 100-101 9.738824139575494 2.0 2.0 22.5 2.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2430565092539787 1101 2 0.2624931182706547 1101 3 0.24777215321405777 1101 4 0.09116268347067802 1101 5 -0.014520875395881205 1101 6 0.1593064043208514 1101 7 0.15657742059133284 1101 8 0.13815621980857173 1101 9 0.10968089259498726 1101 10-11 0.2916713292562463 1101 12-13 0.3959284242886909 1101 14-15 0.599618560775447 1101 16-17 0.576085464043409 1101 18-19 0.4561642058915467 1101 20-21 0.3565947285834028 1101 22-23 0.5063265656127314 1101 24-25 0.5530916694762738 1101 26-27 0.7879197254100063 1101 28-29 0.6633546367559049 1101 30-31 0.7332672500209867 1101 32-33 0.8680219275851044 1101 34-35 0.7575833839779449 1101 36-37 0.912844668707514 1101 38-39 0.9022597381218702 1101 40-41 1.1495716232280913 1101 42-43 1.537182996980853 1101 44-45 1.3448886780305642 1101 46-47 1.6546510802665821 1101 48-49 1.852939838318484 1101 50-51 2.0514082814260988 1101 52-53 2.1166776735023163 1101 54-55 2.252729756380319 1101 56-57 2.3072121267736243 1101 58-59 2.8049726782079496 1101 60-61 2.5894153879532134 1101 62-63 2.8259617552433376 1101 64-65 3.2097295189143367 1101 66-67 3.3589252094646582 1101 68-69 3.7977770814407954 1101 70-71 4.1087037123652586 1101 72-73 4.253816587913072 1101 74-75 4.1863494760683615 1101 76-77 4.045451261616343 1101 78-79 4.532621752664898 1101 80-81 4.635861383419105 1101 82-83 4.747438420859208 1101 84-85 4.689353834270218 1101 86-87 4.8303277160482345 1101 88-89 5.05895448840424 1101 90-91 5.265903589790575 1101 92-93 5.314755535493166 1101 94-95 5.0512535772220435 1101 96-97 4.409610644971096 1101 98-99 4.197059656127166 1101 100-101 3.142691969106629 1102 1 0.31232539507018586 1102 2 0.27991363939597136 1102 3 0.26035642045891905 1102 4 0.19270357023379603 1102 5 0.0161257697858872 1102 6 0.042431434584422334 1102 7 0.076585275670503 1102 8 0.11661985240607464 1102 9 0.18135041972998067 1102 10-11 0.36149733387245675 1102 12-13 0.43016231196105537 1102 14-15 0.4698693314878426 1102 16-17 0.5277075916938188 1102 18-19 0.5995753547237754 1102 20-21 0.5841708986404797 1102 22-23 0.4748424936688167 1102 24-25 0.5436879022663845 1102 26-27 0.5436385996587987 1102 28-29 0.6973103685426096 1102 30-31 0.5964804616185262 1102 32-33 0.7017689079527898 1102 34-35 0.7893996827292042 1102 36-37 0.8887042887584187 1102 38-39 1.1789653932408513 1102 40-41 1.3932160033493588 1102 42-43 1.3554495531742532 1102 44-45 1.316104655046388 1102 46-47 1.4079203534565536 1102 48-49 1.8693494215150679 1102 50-51 2.0096628397931013 1102 52-53 2.0954353335495597 1102 54-55 2.2592774490853174 1102 56-57 2.4095885572319595 1102 58-59 2.769070124231181 1102 60-61 2.6931126553540956 1102 62-63 3.1233674270409146 1102 64-65 2.9126428924556294 1102 66-67 3.2239023167510474 1102 68-69 3.555228377259848 1102 70-71 3.7931883169481075 1102 72-73 4.089087776451112 1102 74-75 4.156398758619865 1102 76-77 3.939597613577689 1102 78-79 4.208754148410243 1102 80-81 4.560870664248949 1102 82-83 4.714999095965599 1102 84-85 5.14368246710983 1102 86-87 5.054865154108786 1102 88-89 5.144784409799648 1102 90-91 5.29849840221021 1102 92-93 5.331518328242819 1102 94-95 5.3875260193446355 1102 96-97 5.041786340495314 1102 98-99 4.467682198052309 1102 100-101 3.4187277904197177 1103 1 0.3574395970235891 1103 2 0.28502652490034563 1103 3 0.3051460670936166 1103 4 0.20420072721021398 1103 5 0.1722991562497569 1103 6 0.14545673572214213 1103 7 0.17293010430428524 1103 8 0.20140777753050543 1103 9 0.22289451317760722 1103 10-11 0.3884627669588667 1103 12-13 0.36079633406726686 1103 14-15 0.6385218952723264 1103 16-17 0.626204814492688 1103 18-19 0.5818035459734432 1103 20-21 0.3492267717753066 1103 22-23 0.5272526080479878 1103 24-25 0.5781082343153372 1103 26-27 0.7307396776280299 1103 28-29 0.7524379375169374 1103 30-31 0.7675920002942789 1103 32-33 0.9671465516102913 1103 34-35 1.2431097905261268 1103 36-37 1.349633347345467 1103 38-39 1.3815714559949583 1103 40-41 1.390945888261335 1103 42-43 1.6418607612187728 1103 44-45 1.7791048423872127 1103 46-47 2.0942829541522414 1103 48-49 2.4338718796292085 1103 50-51 2.446460881322004 1103 52-53 2.5847974127289035 1103 54-55 2.6260338199391313 1103 56-57 2.907867300124405 1103 58-59 3.116293935756193 1103 60-61 3.2055255038966344 1103 62-63 3.240956958233159 1103 64-65 3.320552472660289 1103 66-67 3.556989997319757 1103 68-69 3.8237708150196568 1103 70-71 3.871512983658654 1103 72-73 3.9809513619519485 1103 74-75 3.921541973084601 1103 76-77 3.9921877270454686 1103 78-79 3.9702459889578883 1103 80-81 4.323295411465416 1103 82-83 4.643162497669895 1103 84-85 4.852912546957526 1103 86-87 4.932030079855998 1103 88-89 5.130947264671271 1103 90-91 5.252735849109214 1103 92-93 5.063494622033737 1103 94-95 5.171869642921225 1103 96-97 5.045310120121748 1103 98-99 5.0181191578651685 1103 100-101 4.171737395434377 1104 1 0.2496311772270765 1104 2 0.18961804931279858 1104 3 0.26316622160378245 1104 4 0.24242533260489552 1104 5 0.06127306619373485 1104 6 0.07043620152300889 1104 7 0.1907280245386076 1104 8 0.254744391169865 1104 9 0.17207333893136934 1104 10-11 0.40759153575928764 1104 12-13 0.3680210789395346 1104 14-15 0.5856540812734323 1104 16-17 0.5251353895277546 1104 18-19 0.3826978490892188 1104 20-21 0.4200014728670993 1104 22-23 0.4695689431971033 1104 24-25 0.7355716408227408 1104 26-27 0.5994815898656256 1104 28-29 0.7519618734192477 1104 30-31 0.7855017931700772 1104 32-33 0.8523129173669943 1104 34-35 0.9536027662802766 1104 36-37 1.0805491338827764 1104 38-39 1.1275609913021611 1104 40-41 1.1881401611569657 1104 42-43 1.3009102408899125 1104 44-45 1.339728675231079 1104 46-47 1.3428169866815196 1104 48-49 1.615307995023656 1104 50-51 1.8249347447734579 1104 52-53 2.034397577002494 1104 54-55 2.242158106085128 1104 56-57 2.2544269500356826 1104 58-59 2.43798928572868 1104 60-61 2.420855639577322 1104 62-63 2.744897684136472 1104 64-65 2.9482897201477556 1104 66-67 3.196251579770376 1104 68-69 3.5449251082170257 1104 70-71 3.8404617876384144 1104 72-73 3.947719375868708 1104 74-75 3.8656012936738158 1104 76-77 3.9495792638559237 1104 78-79 4.14327954881314 1104 80-81 4.11046328048047 1104 82-83 4.322402809789718 1104 84-85 4.571637121357192 1104 86-87 4.828173040500072 1104 88-89 4.648471557016272 1104 90-91 4.754119001767425 1104 92-93 4.772450922933366 1104 94-95 4.783806211617803 1104 96-97 4.964734213323766 1104 98-99 4.783082002587882 1104 100-101 3.776409991189608 1105 1 0.20575804944722265 1105 2 0.22373228251695565 1105 3 0.25199927812910516 1105 4 0.19665032667329996 1105 5 0.05730266017098984 1105 6 0.16774033631909901 1105 7 0.2577546357683502 1105 8 0.11581219218444971 1105 9 0.25325481953409223 1105 10-11 0.16920382000909484 1105 12-13 0.2929218423270612 1105 14-15 0.5649593873251675 1105 16-17 0.5467355940267353 1105 18-19 0.3670019405752072 1105 20-21 0.4287103329830231 1105 22-23 0.4932294372334951 1105 24-25 0.5080142737753235 1105 26-27 0.4939537151108979 1105 28-29 0.6064608980005559 1105 30-31 0.6820360910004268 1105 32-33 0.9324144701281014 1105 34-35 0.9877508943425752 1105 36-37 0.9306740740960109 1105 38-39 1.0878491635031082 1105 40-41 0.9726472451823227 1105 42-43 1.0952892023514593 1105 44-45 1.6054547125078926 1105 46-47 1.6120826828254096 1105 48-49 1.720418303956464 1105 50-51 1.8053329246195773 1105 52-53 2.009996103705987 1105 54-55 2.3519762695864017 1105 56-57 2.448505377088299 1105 58-59 2.8817370025333737 1105 60-61 2.753372671743545 1105 62-63 3.0217078107902573 1105 64-65 3.01479566869536 1105 66-67 3.023470186541065 1105 68-69 3.3888228097690316 1105 70-71 3.203469127758254 1105 72-73 3.629029798160982 1105 74-75 3.782975750885324 1105 76-77 3.8175710311714894 1105 78-79 3.679289069502186 1105 80-81 4.255441474788395 1105 82-83 4.368456506777221 1105 84-85 4.2944828677408005 1105 86-87 4.490226004982031 1105 88-89 4.514611582324434 1105 90-91 4.553089539603501 1105 92-93 4.75489691712605 1105 94-95 4.536774836277447 1105 96-97 4.463429025904027 1105 98-99 4.274325830899123 1105 100-101 3.4777485085531925 1106 1 0.2836775801101261 1106 2 0.2016282948787662 1106 3 0.14606632733563885 1106 4 0.2470901923984954 1106 5 0.053871836130142015 1106 6 0.09894471219299561 1106 7 0.2066455090138959 1106 8 0.2321423450350295 1106 9 0.25578579295289927 1106 10-11 0.2532174385753265 1106 12-13 0.32299196544904163 1106 14-15 0.25656739604163903 1106 16-17 0.2870963059698042 1106 18-19 0.1769795798154874 1106 20-21 0.10685181761252238 1106 22-23 0.14807149714269485 1106 24-25 0.24496092765391353 1106 26-27 0.1871664344695816 1106 28-29 0.3821861068632799 1106 30-31 0.5997757801546371 1106 32-33 0.6487139389452281 1106 34-35 0.49626594329922824 1106 36-37 0.6232253412363562 1106 38-39 0.5203911427518761 1106 40-41 0.6410550470112355 1106 42-43 0.7951201186281409 1106 44-45 0.9731881546133749 1106 46-47 1.0589632466031915 1106 48-49 1.2439285665713626 1106 50-51 1.2629503737290975 1106 52-53 1.4320536059521771 1106 54-55 1.445244247765828 1106 56-57 1.7798798472375061 1106 58-59 2.016908438086798 1106 60-61 2.0341301998966053 1106 62-63 2.251277894408844 1106 64-65 2.5315572813158695 1106 66-67 2.7391679175762143 1106 68-69 2.729168455300467 1106 70-71 2.619619953562971 1106 72-73 3.120593755997078 1106 74-75 3.586142499314832 1106 76-77 3.6114774485441643 1106 78-79 3.934747657999896 1106 80-81 3.8562991578315184 1106 82-83 4.218582687974706 1106 84-85 4.014250596956959 1106 86-87 4.091662229045294 1106 88-89 3.9761984337567533 1106 90-91 4.126485012698012 1106 92-93 3.9642844399323707 1106 94-95 4.150853984547469 1106 96-97 3.8448970180474475 1106 98-99 3.5888015748950686 1106 100-101 2.839950556482753 1107 1 0.14857759891537015 1107 2 0.15601729049456026 1107 3 0.10453900995849352 1107 4 0.1524214780501012 1107 5 -0.11633310411712472 1107 6 -0.07437507645084906 1107 7 -0.021144948985892142 1107 8 -0.024735395091290968 1107 9 0.07679309012363689 1107 10-11 0.22539674469882698 1107 12-13 0.2817517967181047 1107 14-15 0.26556164753775846 1107 16-17 0.3314794387753608 1107 18-19 0.235049977785998 1107 20-21 0.12647287358208814 1107 22-23 0.11570758624266375 1107 24-25 0.3555380457323878 1107 26-27 0.39311930706223563 1107 28-29 0.34220238630177136 1107 30-31 0.33635215708539334 1107 32-33 0.5818696057753598 1107 34-35 0.5436500790509413 1107 36-37 0.6500057550253828 1107 38-39 0.7596942795397297 1107 40-41 0.8651761785704863 1107 42-43 0.9473554533061161 1107 44-45 1.0624402222679876 1107 46-47 1.18950065243871 1107 48-49 1.4074728565997958 1107 50-51 1.3743953905247608 1107 52-53 1.496772209497145 1107 54-55 1.8223815002095876 1107 56-57 1.7483243773092987 1107 58-59 2.0073315928686775 1107 60-61 2.090854732623253 1107 62-63 1.79506783249904 1107 64-65 1.9526780552068175 1107 66-67 2.359111243743893 1107 68-69 2.5907876971174204 1107 70-71 2.9461729529988183 1107 72-73 3.174760683847637 1107 74-75 3.172410156882467 1107 76-77 3.4027369756108072 1107 78-79 3.3127684801945065 1107 80-81 3.4352369198604187 1107 82-83 3.785695733280587 1107 84-85 3.573304121833342 1107 86-87 3.9698406834020226 1107 88-89 3.928127538000467 1107 90-91 3.8769772675200826 1107 92-93 3.7086405632504977 1107 94-95 3.610808501676834 1107 96-97 3.574216323654724 1107 98-99 3.289135921007361 1107 100-101 2.322600878568588 1108 1 0.2545393011140682 1108 2 0.17104749329672586 1108 3 0.26327975295429695 1108 4 0.13419078972475518 1108 5 -0.013523767223553307 1108 6 0.051016391343466694 1108 7 0.15343471108895557 1108 8 0.16095758024071216 1108 9 0.2888554539262316 1108 10-11 0.20106313606547133 1108 12-13 0.19680340810707975 1108 14-15 0.3395835550035642 1108 16-17 0.4336511827797267 1108 18-19 0.43855700873908887 1108 20-21 0.37401309570108054 1108 22-23 0.5096926080882582 1108 24-25 0.3281006830345845 1108 26-27 0.5859748295446892 1108 28-29 0.48648963257810607 1108 30-31 0.5783241825439376 1108 32-33 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0.5833570841884139 2311 46-47 0.5648290061948629 2311 48-49 0.43118572806703526 2311 50-51 0.47037746841281347 2311 52-53 0.7015962810109571 2311 54-55 0.6581464098349841 2311 56-57 0.9866823720410878 2311 58-59 1.252825597001749 2311 60-61 1.4232856237080398 2311 62-63 1.4120486333547646 2311 64-65 1.3829060655103973 2311 66-67 2.11640006043336 2311 68-69 2.535752515058448 2311 70-71 2.8705142444386986 2311 72-73 3.1908959510420978 2311 74-75 3.434230437320224 2311 76-77 3.198958451583678 2311 78-79 2.967861510726859 2311 80-81 2.921484718707813 2311 82-83 3.0573343367675 2311 84-85 3.2965364689532883 2311 86-87 3.3720376519964894 2311 88-89 3.3798067809495045 2311 90-91 3.466868428709944 2311 92-93 3.0559196839537037 2311 94-95 2.999769903283495 2311 96-97 3.218225823174471 2311 98-99 2.8072863343146928 2311 100-101 2.450694771370637 2312 1 -0.13692987066725948 2312 2 -0.17971530841285954 2312 3 -0.17830042798825474 2312 4 -0.09467539550603732 2312 5 -0.08749390171816174 2312 6 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68-69 2.878660610028156 2312 70-71 3.2256123492717244 2312 72-73 3.328510876362653 2312 74-75 3.4024216624857857 2312 76-77 3.520678016188441 2312 78-79 3.5091809062460904 2312 80-81 3.4368957198350536 2312 82-83 3.6418788626234964 2312 84-85 3.7002607538624055 2312 86-87 3.8340066177566676 2312 88-89 3.9296984605324283 2312 90-91 3.7695099497751805 2312 92-93 3.529261412853238 2312 94-95 3.3615165039598356 2312 96-97 3.2728655477059654 2312 98-99 3.029617072654837 2312 100-101 2.5048360458528123 2313 1 -0.15067559894917437 2313 2 -0.16005349421580917 2313 3 -0.22996428654619905 2313 4 -0.08469960732261939 2313 5 -0.005085800504971871 2313 6 -0.03391964077452059 2313 7 -0.1628373903319087 2313 8 -0.10658211768834747 2313 9 -0.30813209993200275 2313 10-11 -0.15598276803223854 2313 12-13 -0.16319357766266052 2313 14-15 -0.24254764664609496 2313 16-17 -0.1438884822838915 2313 18-19 -0.1828798494751922 2313 20-21 0.02138688889390039 2313 22-23 -0.017746277454094184 2313 24-25 -0.10061159741840697 2313 26-27 0.0884643865514434 2313 28-29 0.09945612814103555 2313 30-31 0.14209815846859186 2313 32-33 0.165545756510042 2313 34-35 0.23439239876313422 2313 36-37 0.4017530948726957 2313 38-39 0.4140455404784973 2313 40-41 0.419685487427337 2313 42-43 0.8216210203326639 2313 44-45 0.7844613120247104 2313 46-47 0.8920075438289672 2313 48-49 1.0187344753361458 2313 50-51 1.0649015106876973 2313 52-53 1.3650211745584002 2313 54-55 1.6955456973682104 2313 56-57 1.9898665993207665 2313 58-59 2.1479376504924197 2313 60-61 2.253735794519166 2313 62-63 2.521911106045856 2313 64-65 2.5823023995070287 2313 66-67 3.09033114284016 2313 68-69 3.592301521501266 2313 70-71 3.868656108123762 2313 72-73 3.799568659876961 2313 74-75 4.208235097228659 2313 76-77 4.172406627279102 2313 78-79 4.043107551805019 2313 80-81 4.573900817743482 2313 82-83 4.574508508455299 2313 84-85 4.522020636744781 2313 86-87 4.217342694229625 2313 88-89 4.4802439843782516 2313 90-91 4.816467013865029 2313 92-93 4.603407177517845 2313 94-95 4.495675089192796 2313 96-97 4.421806551435193 2313 98-99 4.076033377299261 2313 100-101 3.3305544957767985 2314 1 -0.21109592819079737 2314 2 -0.18433531252730972 2314 3 -0.20418076747429836 2314 4 -0.24823023808448852 2314 5 -0.13709887057526515 2314 6 -0.25608706095722056 2314 7 -0.2520200008618829 2314 8 -0.051787762942012705 2314 9 -0.14378361717282218 2314 10-11 -0.18725177433773865 2314 12-13 -0.18028266430957984 2314 14-15 -0.3403153256711704 2314 16-17 -0.16385172217085398 2314 18-19 -0.3031192224783794 2314 20-21 -0.3979818521284244 2314 22-23 -0.37827095631102026 2314 24-25 -0.25671391018455125 2314 26-27 -0.33072366623891725 2314 28-29 -0.20207665272433672 2314 30-31 -0.1812241966857968 2314 32-33 -0.001842870592241752 2314 34-35 0.17752855858490335 2314 36-37 -0.05551154465644004 2314 38-39 0.1829677697562957 2314 40-41 0.14094125334876395 2314 42-43 0.4954003049023612 2314 44-45 0.5927396427939726 2314 46-47 0.5441364445658579 2314 48-49 0.8454291731739829 2314 50-51 0.9776939349229181 2314 52-53 1.0867751299232182 2314 54-55 1.4147399748249256 2314 56-57 1.6967634864256418 2314 58-59 1.924964049561833 2314 60-61 2.435349729472268 2314 62-63 2.7409400408022755 2314 64-65 3.0077280867166785 2314 66-67 3.008925258714921 2314 68-69 3.2470458627824286 2314 70-71 3.801312134357275 2314 72-73 3.9742388498200647 2314 74-75 4.191469779686383 2314 76-77 4.2541103443991375 2314 78-79 4.171654260793655 2314 80-81 4.077899733614085 2314 82-83 4.201745011627931 2314 84-85 4.574418494940829 2314 86-87 4.790814722351286 2314 88-89 4.923101331019115 2314 90-91 5.04203898073424 2314 92-93 5.116804691195902 2314 94-95 5.365339004758651 2314 96-97 5.069355351306548 2314 98-99 4.751324088670557 2314 100-101 4.104448893447389 2315 1 -0.3786889102252857 2315 2 -0.40233987901171986 2315 3 -0.4204578809754729 2315 4 -0.23179690274830733 2315 5 -0.2441934448707599 2315 6 -0.26418355798300297 2315 7 -0.2605896697917629 2315 8 -0.30411606913681055 2315 9 -0.29652801421661934 2315 10-11 -0.3515137763806422 2315 12-13 -0.356197463324186 2315 14-15 -0.5099249212969639 2315 16-17 -0.2469234340016584 2315 18-19 -0.35619333772202566 2315 20-21 -0.48215012107031896 2315 22-23 -0.29478240256470656 2315 24-25 -0.24443397267328493 2315 26-27 -0.3870386334924163 2315 28-29 -0.29159627058169946 2315 30-31 -0.3363720065758429 2315 32-33 -0.27515221986821103 2315 34-35 -0.23283916358057155 2315 36-37 -0.22764472377077993 2315 38-39 -0.24468950357182706 2315 40-41 -0.2651791876774965 2315 42-43 -0.03751894042601123 2315 44-45 0.06504406507361438 2315 46-47 0.17292933046383752 2315 48-49 0.24064037307784503 2315 50-51 0.20952939127093373 2315 52-53 0.36650234256516967 2315 54-55 0.8643772096984534 2315 56-57 1.0312960226270498 2315 58-59 1.2830124783306829 2315 60-61 1.5442731507304508 2315 62-63 1.4227882652810315 2315 64-65 1.9031195425840117 2315 66-67 2.439845916048334 2315 68-69 2.7239956314539455 2315 70-71 2.9297182210003108 2315 72-73 2.9056477003878705 2315 74-75 3.291767202026108 2315 76-77 3.404353689949822 2315 78-79 3.6725029659549833 2315 80-81 3.807050127172939 2315 82-83 4.134618441898919 2315 84-85 4.483257796749399 2315 86-87 4.3797710403228045 2315 88-89 4.6760755544163395 2315 90-91 4.953398110698792 2315 92-93 4.853717357181601 2315 94-95 5.002232143014973 2315 96-97 4.852244553974337 2315 98-99 4.531021258651933 2315 100-101 4.108223002682504 2316 1 -0.29333035935398044 2316 2 -0.27851432923614183 2316 3 -0.30141476895994757 2316 4 -0.20167164019201778 2316 5 -0.28351272787823234 2316 6 -0.2373045286312987 2316 7 -0.2304002470333657 2316 8 -0.18017676013763406 2316 9 -0.23156831228399 2316 10-11 -0.21701920406071196 2316 12-13 -0.3033121653842201 2316 14-15 -0.3385887418595317 2316 16-17 -0.20371927350831953 2316 18-19 -0.23322576001081785 2316 20-21 -0.2385813850171985 2316 22-23 -0.19092660193638267 2316 24-25 -0.17859695960826372 2316 26-27 -0.27802180440792057 2316 28-29 0.006146655414745794 2316 30-31 -0.003970740481186397 2316 32-33 -0.09073145453256615 2316 34-35 0.14623296158733723 2316 36-37 0.02103134638498716 2316 38-39 0.04673614521977498 2316 40-41 0.03190461737741401 2316 42-43 0.13151224962287955 2316 44-45 0.2698896164509179 2316 46-47 0.3828287491086968 2316 48-49 0.7451361375944288 2316 50-51 0.5664032241013004 2316 52-53 0.8744163442324293 2316 54-55 1.3431995651150288 2316 56-57 1.470129508246064 2316 58-59 1.7904860042841655 2316 60-61 2.277430187603006 2316 62-63 2.308391990031037 2316 64-65 2.740068902438111 2316 66-67 3.019873937220293 2316 68-69 3.6198581629880024 2316 70-71 3.6858154878667087 2316 72-73 3.95786341153773 2316 74-75 4.319707377415526 2316 76-77 4.49843893838176 2316 78-79 4.607159212883484 2316 80-81 4.61606477208035 2316 82-83 4.515902728928911 2316 84-85 4.925214883129961 2316 86-87 5.331697302504143 2316 88-89 5.778856557900099 2316 90-91 5.944846147305597 2316 92-93 5.896728582031713 2316 94-95 5.818943054435673 2316 96-97 5.545465505329073 2316 98-99 5.4970209019030065 2316 100-101 4.562180322041957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 18.0 5 56.0 6 122.0 7 358.0 8 782.0 9 1537.0 10 2605.0 11 4200.0 12 6458.0 13 9525.0 14 13179.0 15 17987.0 16 23398.0 17 29956.0 18 32356.0 19 31269.0 20 33104.0 21 35714.0 22 38724.0 23 41556.0 24 43665.0 25 45594.0 26 48289.0 27 54093.0 28 60610.0 29 67646.0 30 73946.0 31 80152.0 32 87834.0 33 97576.0 34 109318.0 35 119868.0 36 112942.0 37 73235.0 38 15418.0 39 211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.764877444078245 19.41507690435388 15.403300328164866 22.41674532340301 2 16.936014594180726 20.524057329429095 38.67026237540388 23.869665700986296 3 20.32384218000525 23.660616678494936 30.02729110488289 25.988250036616922 4 14.030970179615721 17.23093904669584 35.53334583367238 33.20474494001606 5 13.950875860862396 36.99850759060526 33.912471286879054 15.138145261653296 6 33.056725580250095 35.946672817698555 16.207167131413918 14.789434470637431 7 28.987825776862042 32.91115774696918 19.61460793567459 18.486408540494185 8 27.035592307708633 34.77458252288421 18.72397511941816 19.465850049988997 9 26.34856797569401 16.673789405609792 19.694915637996935 37.282726980699266 10-11 25.68229706915802 26.294510632456763 26.108670826157525 21.914521472227687 12-13 26.532957290453503 23.515156575255038 27.099922593280755 22.851963541010704 14-15 22.949331482363963 25.04355898390555 25.70208237592013 26.30502715781035 16-17 22.28681039274018 28.285090321432577 26.671470979368696 22.756628306458545 18-19 22.250398708284216 26.171608007845393 29.54530981791732 22.03268346595307 20-21 22.614472084759647 25.2488831215144 30.184516674992253 21.9521281187337 22-23 22.517926894286074 25.159351154152716 30.126875960290366 22.195845991270843 24-25 22.47580846017559 25.54821892529845 30.041484351562016 21.934488262963946 26-27 21.789321176676626 25.932420168869974 29.99110186507031 22.287156789383094 28-29 21.958743775268623 26.291574271534845 29.808662476986715 21.941019476209817 30-31 22.109488761874573 25.752026796592975 29.73005291110114 22.408431530431315 32-33 21.881973857189557 25.74700314296752 29.811987994175393 22.55903500566753 34-35 22.735434656080443 25.886746523299774 29.416006354564157 21.961812466055626 36-37 22.838485448626376 25.425191908599924 29.48355731219703 22.252765330576672 38-39 22.21201691470753 25.798290131389013 29.40879898783477 22.58089396606869 40-41 22.653864239215466 25.72232134927905 29.434119544753834 22.18969486675165 42-43 22.42516101367502 25.74725770820685 29.1682410697733 22.659340208344833 44-45 22.449482843713902 26.01054655644849 29.283233947104474 22.256736652733135 46-47 22.935144962529545 26.414238596036903 28.43346504508731 22.217151396346246 48-49 22.783082881794137 26.46963095249888 28.29625971298664 22.451026452720342 50-51 22.895372153675687 26.619031581092383 28.26081111486902 22.224785150362905 52-53 23.271226351681225 26.377365539434265 28.104476429158705 22.246931679725808 54-55 22.701077043035024 26.654197156470538 28.198298467290357 22.446427333204085 56-57 22.839562078605375 26.776906460210864 27.889752659177407 22.49377880200635 58-59 22.827941985998866 26.354228430483246 27.92603059192791 22.89179899158998 60-61 22.53487193928596 26.699622306154186 28.277757101394315 22.487748653165536 62-63 22.520756180155292 26.699091638517697 28.30489190654023 22.47526027478678 64-65 22.234655568401642 26.6785724899067 28.196600330853585 22.890171610838074 66-67 22.263378503826473 26.698740264003984 28.009349669428975 23.02853156274057 68-69 22.83899886612691 26.330761923798775 28.06999234060769 22.76024686946662 70-71 22.689056603506582 26.540113003944278 27.65451543482745 23.116314957721695 72-73 22.703931366912837 26.64534559766506 27.61169238933357 23.03903064608853 74-75 22.68328198829135 27.08018175720328 27.241504718696625 22.995031535808742 76-77 22.66718506998445 26.59921998933004 27.82209049086049 22.91150444982502 78-79 22.69102973578412 26.794788702298288 27.76718407940486 22.746997482512732 80-81 22.897660637686286 26.90505461460222 27.47191438553045 22.725370362181046 82-83 22.853511991081735 26.88703826808497 27.523465811256724 22.735983929576562 84-85 22.761859509702326 26.952538610649903 27.398675458263206 22.88692642138457 86-87 23.026869982597077 26.77520230586049 27.14597403112006 23.05195368042237 88-89 22.668918584263654 27.16300128633835 26.801758137257536 23.36632199214046 90-91 22.74649960234011 26.898401435538055 27.1112768420427 23.243822120079134 92-93 22.817611656008502 26.54993865482122 27.135689591980267 23.49676009719001 94-95 22.64433899485146 26.39573597789875 27.260759909248726 23.699165118001066 96-97 22.865042232344226 26.10501923672224 27.23141555742272 23.798522973510813 98-99 22.563351102444326 26.425019493191183 26.743526208613233 24.268103195751262 100-101 23.234185750654134 25.64805131768312 26.959118780398413 24.158644151264333 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 638.0 1 457.0 2 421.5 3 570.5 4 443.5 5 379.5 6 832.0 7 1847.0 8 2845.0 9 3237.0 10 2624.0 11 1801.5 12 1625.5 13 1760.0 14 1932.5 15 2119.0 16 2293.0 17 2406.5 18 2628.0 19 2852.5 20 3154.0 21 3650.5 22 4261.5 23 5112.5 24 6296.5 25 7701.0 26 9539.0 27 11629.0 28 13253.0 29 14866.5 30 16769.0 31 19449.5 32 23068.0 33 24428.0 34 24301.0 35 25499.5 36 27776.5 37 30113.5 38 31942.5 39 33433.5 40 35103.5 41 37915.5 42 39987.0 43 41169.0 44 43080.0 45 45426.5 46 46809.5 47 48463.0 48 49868.5 49 49448.0 50 48579.5 51 47915.5 52 47901.5 53 49003.0 54 50478.0 55 48604.5 56 44744.0 57 41523.0 58 38511.5 59 34847.0 60 29691.0 61 25242.0 62 21753.5 63 17625.5 64 13678.5 65 10727.5 66 8277.0 67 6463.5 68 5051.5 69 3865.5 70 3050.0 71 2283.0 72 1705.5 73 1352.5 74 1058.0 75 826.5 76 595.0 77 403.5 78 264.0 79 163.0 80 103.0 81 51.0 82 27.5 83 17.0 84 11.0 85 5.5 86 3.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010471841360094077 3 0.0023349376005615172 4 0.0013443580124445098 5 0.10634579435284729 6 7.075568486550052E-5 7 0.0 8 2.1226705459650156E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.537784243275026E-5 16-17 3.537784243275026E-5 18-19 0.0 20-21 0.0 22-23 3.184005818947523E-4 24-25 7.075568486550052E-4 26-27 0.006827923589520801 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01107326468145083 36-37 7.783125335205057E-4 38-39 0.007500102595743055 40-41 0.010224196463064826 42-43 0.005660454789240042 44-45 0.003997696194900779 46-47 3.537784243275026E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 7.075568486550052E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4151136973100103E-4 68-69 0.0011674688002807586 70-71 3.537784243275026E-5 72-73 1.0613352729825078E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0613352729825078E-4 82-83 0.002441071127859768 84-85 0.004988275783017786 86-87 0.003219383661380274 88-89 0.0 90-91 0.003254761503813024 92-93 0.0 94-95 3.537784243275026E-5 96-97 1.0613352729825078E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1413314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.64287378730144 #Duplication Level Percentage of deduplicated Percentage of total 1 81.84084825960265 49.63064231652773 2 10.946085713596512 13.276041887892335 3 3.0231366824437567 5.499950888255938 4 1.326629742388418 3.218025601205642 5 0.6900615090050497 2.0923656498034005 6 0.45506087172365933 1.6557719405686349 7 0.29021002414493335 1.2319418906221629 8 0.22287521120327997 1.0812634642654928 9 0.1673061336785021 0.9131332273656109 >10 0.9343376466564141 10.349894037613026 >50 0.0633039435414181 2.6120323281455327 >100 0.03382424312407409 3.8888820578541807 >500 0.004681495475104176 1.9743779546928761 >1k 0.001404448642531253 1.5348308027260567 >5k 2.3407477375520883E-4 1.0408459524613929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7756 0.548781091816822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6909 0.4888510267357431 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4319 0.3055938029340967 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1910 0.135143358093106 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1863 0.13181784090442747 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1805 0.12771401118222844 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1785 0.12629889748491843 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1752 0.12396395988435689 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1667 0.11794972667078937 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1518 0.10740712962582978 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.075568486550052E-5 7 0.0 0.0 0.0 0.0 7.075568486550052E-5 8 0.0 0.0 0.0 0.0 7.075568486550052E-5 9 0.0 0.0 0.0 0.0 7.075568486550052E-5 10-11 1.0613352729825078E-4 0.0 0.0 0.0 7.075568486550052E-5 12-13 1.4151136973100103E-4 0.0 0.0 0.0 1.4151136973100103E-4 14-15 1.4151136973100103E-4 0.0 0.0 0.0 1.4151136973100103E-4 16-17 1.4151136973100103E-4 0.0 0.0 7.075568486550052E-5 1.4151136973100103E-4 18-19 1.768892121637513E-4 0.0 0.0 7.075568486550052E-5 1.4151136973100103E-4 20-21 2.1226705459650156E-4 0.0 0.0 7.075568486550052E-5 1.768892121637513E-4 22-23 2.1226705459650156E-4 0.0 0.0 1.0613352729825078E-4 2.1226705459650156E-4 24-25 4.245341091930031E-4 0.0 0.0 3.537784243275026E-4 2.1226705459650156E-4 26-27 4.245341091930031E-4 0.0 0.0 7.075568486550052E-4 2.1226705459650156E-4 28-29 8.844460608187565E-4 0.0 0.0 0.0019457813338012641 2.1226705459650156E-4 30-31 9.198239032515067E-4 0.0 0.0 0.005872721843836543 2.1226705459650156E-4 32-33 9.198239032515067E-4 0.0 0.0 0.015672384197708365 2.1226705459650156E-4 34-35 0.0010259574305497574 0.0 0.0 0.030460322334597972 2.1226705459650156E-4 36-37 0.0010613352729825077 0.0 0.0 0.05745361611078642 2.1226705459650156E-4 38-39 0.0011320909578480083 0.0 0.0 0.09955324860575923 2.1226705459650156E-4 40-41 0.0011320909578480083 0.0 0.0 0.14497839828941056 2.1226705459650156E-4 42-43 0.0012028466427135088 0.0 0.0 0.1908988377671204 2.1226705459650156E-4 44-45 0.0013443580124445098 0.0 0.0 0.24955530052062036 2.1226705459650156E-4 46-47 0.0014504915397427606 0.0 0.0 0.3215138320288343 2.1226705459650156E-4 48-49 0.0015566250670410115 0.0 0.0 0.39467521017976187 2.1226705459650156E-4 50-51 0.0015566250670410115 0.0 0.0 0.47480602328994126 2.8302273946200206E-4 52-53 0.0015566250670410115 0.0 0.0 0.5500546941444011 3.1840058189475237E-4 54-55 0.0015566250670410115 0.0 0.0 0.6352445387224637 3.537784243275026E-4 56-57 0.001627380751906512 0.0 0.0 0.7337364520552404 3.8915626676025287E-4 58-59 0.001627380751906512 0.0 0.0 0.8352354819948009 4.245341091930031E-4 60-61 0.001627380751906512 0.0 0.0 0.9464987964458005 4.245341091930031E-4 62-63 0.001627380751906512 0.0 0.0 1.0534460141200044 4.245341091930031E-4 64-65 0.001627380751906512 0.0 0.0 1.1657706638439866 4.245341091930031E-4 66-67 0.0016981364367720125 0.0 0.0 1.2928478738624256 4.245341091930031E-4 68-69 0.0016981364367720125 0.0 0.0 1.4309275928774496 4.245341091930031E-4 70-71 0.0016981364367720125 0.0 0.0 1.5760121246941585 5.660454789240041E-4 72-73 0.0017335142792047628 0.0 0.0 1.727252401094166 5.660454789240041E-4 74-75 0.001768892121637513 0.0 0.0 1.8771836973241616 5.660454789240041E-4 76-77 0.0018042699640702631 0.0 0.0 2.0384005252902044 5.660454789240041E-4 78-79 0.0018396478065030135 0.0 0.0 2.212459510059336 5.660454789240041E-4 80-81 0.0019811591762340145 0.0 0.0 2.4079928451851464 6.014233213567544E-4 82-83 0.0019811591762340145 0.0 0.0 2.6011558648679625 6.368011637895046E-4 84-85 0.002016537018666765 0.0 0.0 2.7884461627069426 6.368011637895046E-4 86-87 0.0020872927035322656 0.0 0.0 2.9814322931775954 6.368011637895046E-4 88-89 0.002193426230830516 0.0 7.075568486550052E-5 3.1818831484015586 6.368011637895046E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4160 0.0 46.74762 1 GTATCAA 6725 0.0 38.745193 1 TATCAAC 8440 0.0 30.7032 2 ATCAACG 8540 0.0 30.286928 3 TCAACGC 8695 0.0 29.747023 4 CAACGCA 8825 0.0 29.307785 5 AACGCAG 9040 0.0 28.633936 6 GGACCGA 525 0.0 27.14032 6 ACGCAGA 10315 0.0 24.956465 7 CGCAGAG 10295 0.0 24.866543 8 ACCGAGT 540 0.0 24.627329 8 TGGTATC 2055 0.0 24.29445 2 AGGACCG 665 0.0 23.593431 5 GTGTAGG 610 0.0 23.384117 1 GTGTAAG 615 0.0 23.194004 1 GTGGTAT 2260 0.0 22.9323 1 GAGTACT 6995 0.0 22.916016 12-13 GCAGAGT 11055 0.0 22.856298 9 TAAGGTG 925 0.0 22.101692 5 GTACTTT 7200 0.0 22.032663 14-15 >>END_MODULE