FastQCFastQC Report
Thu 26 May 2016
SRR937922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405549
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77990.554872153158659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64530.45910886066583234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.28245191025001615No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21370.15204023481216236No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20910.14876749227526043No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19350.13766862628054946No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17420.12393733694093909No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.12244325882626646No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA15910.11319420383067398No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC15270.10864082290976693No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14660.1043008817195274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39650.046.6848261
GTATCAA63750.036.819011
CGCGTAA300.00395245231.66578910-11
TATCAAC79700.029.4649642
ATCAACG79550.029.3412483
TCAACGC81150.028.7627414
CAACGCA81950.028.4239465
AACGCAG83200.028.0480466
ACGCAGA95000.024.5010667
CGCAGAG95100.024.37548
GAGTACT62750.022.55874812-13
TAAGGTG8650.022.5322745
GTGGTAT21600.022.5285721
TACATGG49800.022.241492
GCAGAGT102300.022.19479
GTACATG50400.021.8659651
TGGTATC21800.021.5881792
GTACTTT65100.021.34613414-15
GTAAGGT8300.021.191464
AGGTAAG8750.021.188192
Produced by FastQC (version 0.11.5)