##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937922_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1405549 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.381725574846556 33.0 31.0 34.0 31.0 34.0 2 32.592001417239814 34.0 31.0 34.0 31.0 34.0 3 32.677336755957995 34.0 31.0 34.0 31.0 34.0 4 36.167560860560535 37.0 37.0 37.0 35.0 37.0 5 36.17934344515915 37.0 37.0 37.0 35.0 37.0 6 36.22572318716743 37.0 37.0 37.0 35.0 37.0 7 36.19027155936933 37.0 37.0 37.0 35.0 37.0 8 36.1714931318652 37.0 37.0 37.0 35.0 37.0 9 37.90335448995375 39.0 38.0 39.0 35.0 39.0 10-11 37.83414701301769 39.0 38.0 39.0 35.0 39.0 12-13 37.75557131056975 39.0 38.0 39.0 35.0 39.0 14-15 39.06394156304761 40.0 39.0 41.0 36.0 41.0 16-17 38.92681329501853 40.0 38.0 41.0 36.0 41.0 18-19 39.06519480999951 40.0 39.0 41.0 36.0 41.0 20-21 39.051385259425324 40.0 39.0 41.0 36.0 41.0 22-23 38.979260417103916 40.0 39.0 41.0 36.0 41.0 24-25 38.87880963239275 40.0 39.0 41.0 35.5 41.0 26-27 38.718306512259616 40.0 38.0 41.0 35.0 41.0 28-29 38.52705313012922 40.0 38.0 41.0 35.0 41.0 30-31 38.308916658188366 40.0 38.0 41.0 34.0 41.0 32-33 38.05560852022946 40.0 38.0 41.0 34.0 41.0 34-35 37.826860536345585 40.0 37.5 41.0 33.5 41.0 36-37 37.577863169480395 40.0 37.0 41.0 33.0 41.0 38-39 37.291367999265766 40.0 37.0 41.0 32.5 41.0 40-41 37.079353690266224 40.0 36.5 41.0 32.0 41.0 42-43 36.90857309136857 39.5 36.0 41.0 31.5 41.0 44-45 36.64150093664468 39.0 36.0 41.0 31.0 41.0 46-47 36.25254758105196 39.0 35.5 41.0 30.0 41.0 48-49 35.83552974673953 39.0 35.0 40.0 28.5 41.0 50-51 35.11865932813441 38.5 34.5 39.5 27.5 40.5 52-53 35.22885612668075 38.0 35.0 40.0 28.0 40.5 54-55 35.33311645485145 38.5 35.0 40.0 28.0 41.0 56-57 35.03480134808534 38.0 35.0 40.0 27.0 41.0 58-59 34.72095743371452 38.0 34.5 40.0 26.0 41.0 60-61 34.30692241963816 37.5 34.0 40.0 26.0 41.0 62-63 33.789005221447276 37.0 33.5 40.0 24.0 41.0 64-65 33.28495840415383 36.0 33.0 39.0 22.5 41.0 66-67 32.774064796033436 35.5 32.5 39.0 20.5 40.0 68-69 32.19718487224565 35.0 32.0 38.5 18.5 40.0 70-71 31.65313624782914 35.0 31.0 37.5 14.5 40.0 72-73 31.04684432915537 35.0 31.0 37.0 7.5 39.0 74-75 30.390438184652403 34.5 30.0 36.5 4.5 39.0 76-77 29.765015662918902 34.0 29.0 36.0 2.0 38.5 78-79 29.077894829707112 34.0 29.0 35.0 2.0 37.5 80-81 28.466078379337894 34.0 27.5 35.0 2.0 37.0 82-83 27.762613043017353 33.0 26.0 35.0 2.0 36.0 84-85 27.144227273471078 33.0 25.5 35.0 2.0 36.0 86-87 26.49666002394794 32.5 24.5 35.0 2.0 35.5 88-89 25.715802864218894 32.0 21.5 34.5 2.0 35.0 90-91 24.920052235816755 31.0 19.0 34.0 2.0 35.0 92-93 24.062873297195615 31.0 11.5 34.0 2.0 35.0 94-95 23.12907020673061 30.5 2.0 34.0 2.0 35.0 96-97 21.946147377288163 29.5 2.0 34.0 2.0 35.0 98-99 20.4539340143958 29.0 2.0 34.0 2.0 35.0 100-101 16.57906127783521 20.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2033984159488611 1101 2 0.17020653561510812 1101 3 0.09703569610564244 1101 4 0.03131377642408495 1101 5 0.06023622449542643 1101 6 0.069822342117412 1101 7 0.04316888243623396 1101 8 0.08167438242512759 1101 9 0.06567647052427361 1101 10-11 0.16862546415025292 1101 12-13 0.2354516179200843 1101 14-15 0.34964559083543634 1101 16-17 0.43043156536290184 1101 18-19 0.2822444336472998 1101 20-21 0.4037806970341009 1101 22-23 0.3672937194227117 1101 24-25 0.5309758554747503 1101 26-27 0.5014602610576802 1101 28-29 0.4633327693335261 1101 30-31 0.480232812990252 1101 32-33 0.47628818392289674 1101 34-35 0.5272456028555439 1101 36-37 0.6865771394828926 1101 38-39 0.680913971776576 1101 40-41 0.6433282288363174 1101 42-43 0.8522615236203066 1101 44-45 0.9101341632556341 1101 46-47 0.8367394849650438 1101 48-49 0.9425999271637551 1101 50-51 0.7941801230129357 1101 52-53 0.9034393017275661 1101 54-55 0.912242312439588 1101 56-57 1.0875085453441216 1101 58-59 1.2310592492910004 1101 60-61 1.0626627182429829 1101 62-63 1.097414840663582 1101 64-65 1.102627595688098 1101 66-67 1.2458326092951708 1101 68-69 1.5232370011166765 1101 70-71 1.6671460747327806 1101 72-73 1.8145662878009432 1101 74-75 1.8948904631859627 1101 76-77 1.995837726289313 1101 78-79 1.9309071515490182 1101 80-81 2.0958865057345335 1101 82-83 2.0039333314757783 1101 84-85 2.2845224770814276 1101 86-87 2.3889853887615224 1101 88-89 2.9017109653574487 1101 90-91 3.137004432849441 1101 92-93 3.568761711506287 1101 94-95 3.505999362264067 1101 96-97 3.8350673351976 1101 98-99 4.26172558846805 1101 100-101 4.6454491997304785 1102 1 0.17933512825123188 1102 2 0.06707902450379777 1102 3 0.0794407032710609 1102 4 -0.009720525261869284 1102 5 0.15336494119238608 1102 6 0.17388102942128825 1102 7 0.08820835149429485 1102 8 0.08201688981122857 1102 9 0.17906205262794117 1102 10-11 0.17105454267422715 1102 12-13 0.15304075937664408 1102 14-15 0.21721831312348172 1102 16-17 0.21771329256863226 1102 18-19 0.25778567350386083 1102 20-21 0.36475477996931005 1102 22-23 0.2816227353470282 1102 24-25 0.18061551651854302 1102 26-27 0.2022876257332129 1102 28-29 0.29207352756115057 1102 30-31 0.35140816819664167 1102 32-33 0.24704254256899816 1102 34-35 0.11948904641169378 1102 36-37 0.2210420082632183 1102 38-39 0.45993239302434574 1102 40-41 0.5260028029341797 1102 42-43 0.685068232931414 1102 44-45 0.5681955210073681 1102 46-47 0.5412552355127289 1102 48-49 0.8065057018181108 1102 50-51 0.7225658135688633 1102 52-53 0.647640762655243 1102 54-55 0.7848323391692915 1102 56-57 0.6924921034762974 1102 58-59 0.6758845377362022 1102 60-61 0.6662497643008933 1102 62-63 0.7682322025092674 1102 64-65 0.747108207701892 1102 66-67 0.8378455287342987 1102 68-69 1.0696306102012372 1102 70-71 1.2090217642243637 1102 72-73 1.3528865084987842 1102 74-75 1.2408403259560181 1102 76-77 1.575886343169831 1102 78-79 1.6905877772832767 1102 80-81 1.6252346975326155 1102 82-83 2.006272873827399 1102 84-85 2.1102139611573563 1102 86-87 2.2901712512269015 1102 88-89 2.6711338832883413 1102 90-91 2.9481918136465275 1102 92-93 3.0256330200307886 1102 94-95 3.353074102016464 1102 96-97 3.5331200873882125 1102 98-99 3.5962294496209033 1102 100-101 3.9096800285226934 1103 1 0.28809402441016374 1103 2 0.2543369157569586 1103 3 0.23079506547840367 1103 4 0.10403577273037001 1103 5 0.11063564846038076 1103 6 0.07229271164680995 1103 7 0.18629185693356476 1103 8 0.2619275755705033 1103 9 0.11005751819408971 1103 10-11 0.22999736009966654 1103 12-13 0.2855162154423141 1103 14-15 0.3993407560994484 1103 16-17 0.3872237762616706 1103 18-19 0.3342284396893618 1103 20-21 0.3870336072617562 1103 22-23 0.38847429985329995 1103 24-25 0.41977887067337605 1103 26-27 0.3750449135606502 1103 28-29 0.475901055107137 1103 30-31 0.5286733656633373 1103 32-33 0.41854686300070654 1103 34-35 0.4793872778498667 1103 36-37 0.5214545802553445 1103 38-39 0.35527992645388906 1103 40-41 0.5225709321697636 1103 42-43 0.6715463124823344 1103 44-45 0.670865001651336 1103 46-47 0.6445254291756086 1103 48-49 0.5943427853126977 1103 50-51 0.710257074204165 1103 52-53 0.538492442903511 1103 54-55 0.6123647077448524 1103 56-57 0.6488573707498873 1103 58-59 0.7584670551487349 1103 60-61 0.6369058526313296 1103 62-63 0.7172981887094494 1103 64-65 0.9085387546672195 1103 66-67 0.8234932197417564 1103 68-69 1.0556556683435119 1103 70-71 1.2828181378829306 1103 72-73 1.504767135097012 1103 74-75 1.5023931254052805 1103 76-77 1.514799284692394 1103 78-79 1.6908153429276744 1103 80-81 1.7386974207730042 1103 82-83 1.8678156061342435 1103 84-85 1.7738092451532559 1103 86-87 1.7164966477939423 1103 88-89 2.137608791990811 1103 90-91 2.356077761965313 1103 92-93 2.5219518819976052 1103 94-95 2.6911013158534196 1103 96-97 2.7581665348925846 1103 98-99 2.9570189547153625 1103 100-101 3.322236347252037 1104 1 0.1548431658611804 1104 2 0.06826276056014535 1104 3 0.14750342021912388 1104 4 0.05009816513006626 1104 5 0.07792177737342598 1104 6 0.05241538725501016 1104 7 0.05261623071109511 1104 8 0.09528231629321482 1104 9 0.19750895722133777 1104 10-11 0.09538600562034105 1104 12-13 0.17763593007053657 1104 14-15 0.25149400145968315 1104 16-17 0.33094543963836287 1104 18-19 0.27708141373909 1104 20-21 0.266799568160593 1104 22-23 0.29935139439734115 1104 24-25 0.2699012308042512 1104 26-27 0.3269780008216756 1104 28-29 0.4257114307054124 1104 30-31 0.4491359004785096 1104 32-33 0.48480498654162574 1104 34-35 0.6129637540583701 1104 36-37 0.48450542618523684 1104 38-39 0.5564153067843236 1104 40-41 0.5187508398701155 1104 42-43 0.458471242401302 1104 44-45 0.45762993950229713 1104 46-47 0.5989023389400856 1104 48-49 0.7203562561941368 1104 50-51 0.7107263467134786 1104 52-53 0.5408517625541407 1104 54-55 0.6936046237377482 1104 56-57 0.8939736692094513 1104 58-59 0.9776635118877763 1104 60-61 0.9514964532100052 1104 62-63 1.03417261972357 1104 64-65 1.1916137346118703 1104 66-67 1.1579724117592747 1104 68-69 1.29227830339034 1104 70-71 1.3921175078296564 1104 72-73 1.6581155888074761 1104 74-75 1.6775399350282392 1104 76-77 1.6518792491786627 1104 78-79 1.8936869752073164 1104 80-81 1.8718203983380626 1104 82-83 2.1865609303097244 1104 84-85 2.201620711401688 1104 86-87 2.3922884958600683 1104 88-89 2.6227565705798206 1104 90-91 2.740056471753224 1104 92-93 3.0983548546952946 1104 94-95 3.054406121023643 1104 96-97 3.2574212197307446 1104 98-99 3.32216085190581 1104 100-101 3.6253790646835107 1105 1 0.19029774909063235 1105 2 0.09789654542953485 1105 3 0.06740771119671507 1105 4 0.01117741940304029 1105 5 0.03202022885407274 1105 6 0.12148239742149514 1105 7 0.14232439437213174 1105 8 0.08072512634335283 1105 9 0.14203304376113124 1105 10-11 0.18116054231110468 1105 12-13 0.21298316960626096 1105 14-15 0.2498440250060412 1105 16-17 0.2696746980665736 1105 18-19 0.25573733218558914 1105 20-21 0.3186725424812167 1105 22-23 0.20013811979139717 1105 24-25 0.26347619132947386 1105 26-27 0.31501610437025107 1105 28-29 0.21014798019432845 1105 30-31 0.18581835705626304 1105 32-33 0.15344712344292333 1105 34-35 0.3493986276005714 1105 36-37 0.23565157981829543 1105 38-39 0.32205459042098994 1105 40-41 0.4335701561946692 1105 42-43 0.37006159116442205 1105 44-45 0.30493699786956796 1105 46-47 0.24430456614859253 1105 48-49 0.25083667116408037 1105 50-51 0.24647872212553779 1105 52-53 0.09385703970863091 1105 54-55 0.3620926704980505 1105 56-57 0.3643554664891653 1105 58-59 0.2354615118323764 1105 60-61 0.25939452517875594 1105 62-63 0.3546541427574468 1105 64-65 0.3885495915608246 1105 66-67 0.5587764506523243 1105 68-69 0.755682760577514 1105 70-71 0.655777858531895 1105 72-73 0.6179569279027461 1105 74-75 0.799726491442545 1105 76-77 0.8702544119255542 1105 78-79 1.197994227485772 1105 80-81 1.2554823418822032 1105 82-83 1.2983195662830198 1105 84-85 1.2935380775337144 1105 86-87 1.4483409488366057 1105 88-89 1.849310630249061 1105 90-91 1.9418781981168358 1105 92-93 2.342805878269356 1105 94-95 2.3760632306388665 1105 96-97 2.3634421621391226 1105 98-99 2.662854153659321 1105 100-101 2.689445936342004 1106 1 0.068279182062021 1106 2 0.10714997508026869 1106 3 0.06702576687338535 1106 4 0.09965595552729667 1106 5 0.08514667858566582 1106 6 0.08310832917379685 1106 7 0.0943877657371246 1106 8 0.11521391392301439 1106 9 -0.03485949908115771 1106 10-11 0.03493886984841765 1106 12-13 0.14352881805703532 1106 14-15 0.24113560723219507 1106 16-17 0.07367273079169934 1106 18-19 0.052137681341996256 1106 20-21 0.14328270638590368 1106 22-23 0.09659392935640199 1106 24-25 0.08975016749197096 1106 26-27 0.06517568384780503 1106 28-29 0.12362908877464207 1106 30-31 0.05439244902287044 1106 32-33 0.04946866754441004 1106 34-35 0.06863924729216109 1106 36-37 0.13431119940872804 1106 38-39 -0.18119150031782283 1106 40-41 0.07447273074357241 1106 42-43 0.23807606493583933 1106 44-45 0.1959695032499269 1106 46-47 0.40503489078383126 1106 48-49 0.5069229277957916 1106 50-51 0.6588889913701266 1106 52-53 0.599878548929361 1106 54-55 0.2005188854821256 1106 56-57 0.683210958988056 1106 58-59 0.8614880962037077 1106 60-61 0.9350219864523908 1106 62-63 0.7578920801185802 1106 64-65 0.8013489992855156 1106 66-67 0.7789036956652424 1106 68-69 0.5604994011417475 1106 70-71 0.9051670092202677 1106 72-73 1.1181208674753123 1106 74-75 1.1016233943798532 1106 76-77 1.2007445999677309 1106 78-79 1.4317742380197593 1106 80-81 1.6483822759575695 1106 82-83 1.8361095998034287 1106 84-85 2.000631908522479 1106 86-87 1.79003876111533 1106 88-89 2.066672266993848 1106 90-91 2.4438905999710947 1106 92-93 2.593598325538366 1106 94-95 2.584202394407523 1106 96-97 2.8204208866194875 1106 98-99 3.211031895548672 1106 100-101 3.804683029588741 1107 1 0.1718068714213743 1107 2 0.040914296808175266 1107 3 -0.019871034436029333 1107 4 -0.003740134322264055 1107 5 0.051942477271317955 1107 6 0.05076774964246056 1107 7 0.07365261797554723 1107 8 0.09582766113680918 1107 9 -0.005208484531188162 1107 10-11 0.0868055266451222 1107 12-13 0.0637342777916956 1107 14-15 0.13357768288547334 1107 16-17 0.1337684694350756 1107 18-19 0.09516249607455762 1107 20-21 0.12135493806684394 1107 22-23 0.17832544990648813 1107 24-25 0.14975131898768268 1107 26-27 0.292337000171905 1107 28-29 0.36108806673994565 1107 30-31 0.32740796954006157 1107 32-33 0.3464846486598887 1107 34-35 0.31556516499311726 1107 36-37 0.354156405485341 1107 38-39 0.4232409356713589 1107 40-41 0.41784463918321535 1107 42-43 0.43197560516004785 1107 44-45 0.5336127205289358 1107 46-47 0.629876765747845 1107 48-49 0.6062805576886205 1107 50-51 0.5576642934321185 1107 52-53 0.5528029367860157 1107 54-55 0.7865685149472554 1107 56-57 0.9037840748531991 1107 58-59 0.9096288418794316 1107 60-61 0.9733914685260743 1107 62-63 1.0399509544492744 1107 64-65 1.0627673130022899 1107 66-67 1.0263354489819605 1107 68-69 1.1294986691194708 1107 70-71 1.2987359056761285 1107 72-73 1.4181348499232342 1107 74-75 1.5229926872792383 1107 76-77 1.50524530320261 1107 78-79 1.5875222578583887 1107 80-81 1.7302453960174446 1107 82-83 1.989871289541135 1107 84-85 2.0441378839627227 1107 86-87 2.3124476901420685 1107 88-89 2.4043401591809506 1107 90-91 2.75592948762624 1107 92-93 3.1257497307743805 1107 94-95 3.2241708105251767 1107 96-97 3.21806936787889 1107 98-99 3.2931705520821772 1107 100-101 3.364663618661048 1108 1 0.09816680574970604 1108 2 0.03696606037031813 1108 3 0.10730086761199686 1108 4 0.10415931461106709 1108 5 0.1163976851241415 1108 6 0.1384548072638907 1108 7 0.10113899011171412 1108 8 0.0476279279899714 1108 9 0.13031621922707615 1108 10-11 0.18825375068274752 1108 12-13 0.22998305636805583 1108 14-15 0.32253130102751015 1108 16-17 0.29057880528318236 1108 18-19 0.20198498415793154 1108 20-21 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2316 72-73 -0.8175123120421119 2316 74-75 -1.0534989751546 2316 76-77 -0.7726242228179885 2316 78-79 -1.8593036518453836 2316 80-81 -1.8695605143423073 2316 82-83 -1.4070309440631874 2316 84-85 -1.1213963345860272 2316 86-87 -1.3124699802149138 2316 88-89 -1.629717497719625 2316 90-91 -1.5661613617497387 2316 92-93 -2.1146811934362475 2316 94-95 -2.1054094878028806 2316 96-97 -2.62073362337976 2316 98-99 -2.59272876242105 2316 100-101 -2.6819679797883627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2866.0 3 583.0 4 634.0 5 621.0 6 749.0 7 868.0 8 1133.0 9 1532.0 10 1944.0 11 2697.0 12 3702.0 13 5035.0 14 6659.0 15 8767.0 16 10916.0 17 14626.0 18 13971.0 19 10605.0 20 10307.0 21 10629.0 22 11621.0 23 13272.0 24 14872.0 25 16644.0 26 19711.0 27 22785.0 28 27312.0 29 33199.0 30 40400.0 31 50389.0 32 64792.0 33 85668.0 34 117701.0 35 167410.0 36 223586.0 37 249786.0 38 129721.0 39 7835.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00154328705854 17.998656768671275 14.440737062467402 23.559062881802788 2 15.77376652234783 19.221261915556752 40.33534734386199 24.669624218233427 3 19.526118842566593 23.017267826776486 29.44634616657662 28.0102671640803 4 12.904725261974741 16.01959267118495 35.973451232941656 35.10223083389865 5 14.061040351796578 36.66095149126205 33.80310271366402 15.47490544327736 6 33.15817430174917 35.212020751361045 16.67528221252914 14.954522734360648 7 29.220684010107124 32.5754653190505 19.660272607566107 18.54357806327627 8 26.985178774273816 34.28191360643183 19.006599231892682 19.726308387401673 9 26.362920061525628 16.655622327738563 19.807992210360137 37.17346540037567 10-11 25.517412698062458 25.948575117639898 26.357565503589875 22.176446680707766 12-13 26.42297674289495 23.366751638915748 27.07427248368914 23.135999134500167 14-15 22.91076824968435 24.8888642157721 25.64492917629274 26.55543835825081 16-17 22.34375322500404 28.010329977075337 26.595171515056187 23.050745282864437 18-19 22.309086845484334 26.043182161129508 29.30301964792602 22.344711345460137 20-21 22.614266865838868 25.17741666248462 29.89387566828218 22.314440803394326 22-23 22.415220955612426 25.075991249395436 29.85440927191112 22.654378523081018 24-25 22.342978574962714 25.43980237280775 29.826862632727373 22.390356419502158 26-27 21.733505314987674 25.854551641122125 29.67875877081523 22.733184273074976 28-29 21.927564717827387 26.30457922602315 29.49914392854766 22.268712127601802 30-31 22.035918426032264 25.67061003243214 29.47036283211981 22.823108709415784 32-33 21.68626461482834 25.735621478748556 29.59319236286804 22.98492154355506 34-35 22.65085145335015 25.916155832619165 29.160248616975537 22.272744097055146 36-37 22.73507625489427 25.41077711164525 29.190389185734244 22.663757447726233 38-39 22.104288215688527 25.80426505191567 29.137855760502955 22.953590971892844 40-41 22.51241090337713 25.66882956072234 29.258348894404847 22.560410641495686 42-43 22.241768063684336 25.714724885758233 29.028100823807407 23.015406226750027 44-45 22.214141977645394 26.122284302535743 29.114141123764366 22.5494325960545 46-47 22.71198997138086 26.47240190481138 28.321668741729255 22.493939382078505 48-49 22.582063884774126 26.437894903145203 28.226112062557533 22.753929149523135 50-51 22.65684983217596 26.744896790174057 28.11822895653083 22.480024421119143 52-53 22.96637456549506 26.324257205577318 28.165668683979266 22.543699544948357 54-55 22.38045749510879 26.57766718267961 28.29388757701502 22.74798774519658 56-57 22.539613980496583 26.750043672596767 27.99927562193869 22.711066724967953 58-59 22.53086672314107 26.312184844201454 28.000475324232678 23.1564731084248 60-61 22.198135310096756 26.630411772344882 28.383917627741578 22.787535289816788 62-63 22.216936792826743 26.600957354530713 28.533491398111906 22.64861445453064 64-65 21.94975989309132 26.686325416108815 28.36445869830283 22.999455992497037 66-67 21.975297046645874 26.576736190417233 28.289499430418967 23.15846733251792 68-69 22.493712601586534 26.274192119556805 28.401643528969352 22.83045174988731 70-71 22.35237985788845 26.49343967801996 28.001831631507617 23.15234883258397 72-73 22.263094190219142 26.616783020727414 28.058287798584963 23.061834990468476 74-75 22.271659297356297 27.05300637977825 27.785976441925232 22.889357880940217 76-77 22.49576726597935 26.677519922044407 27.79854071625082 23.028172095725427 78-79 22.530483379300335 26.87393799151247 27.766736684512676 22.828841944674515 80-81 22.77313429862038 26.975214115544123 27.475848848230576 22.775802737604923 82-83 22.66229526860794 27.083194725859617 27.497789692584927 22.756720312947508 84-85 22.581107673106608 27.10255203346878 27.470996061889775 22.84534423153484 86-87 22.741534869060924 27.080379167286257 27.194110854421304 22.983975109231515 88-89 22.636596315823372 27.211625057866602 27.03275009491724 23.11902853139279 90-91 22.674694379355685 27.206829249198922 26.995176611458348 23.12329975998705 92-93 22.791381455520582 26.7632924493629 27.25930100543044 23.186025089686073 94-95 22.66449080912508 26.523103244464068 27.45754533520886 23.35486061120199 96-97 22.980199985624832 26.23183444930654 27.341675674867865 23.44628989020076 98-99 22.67108723869381 26.501336418909688 26.991285523978686 23.836290818417815 100-101 23.47305640918594 25.532408289699926 27.042404937713975 23.95213036340016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 727.0 1 519.0 2 461.5 3 633.5 4 507.5 5 410.5 6 810.5 7 1802.0 8 2933.0 9 3335.5 10 2621.0 11 1789.0 12 1659.0 13 1762.0 14 1843.5 15 1959.0 16 2040.5 17 2173.5 18 2358.5 19 2544.0 20 2816.0 21 3260.0 22 3929.5 23 4700.0 24 5641.5 25 7005.0 26 8940.5 27 10963.5 28 12501.0 29 14157.5 30 16089.0 31 18743.5 32 22602.5 33 24069.5 34 24021.5 35 25372.0 36 27377.5 37 29860.0 38 32037.0 39 33464.0 40 35168.0 41 37992.5 42 39894.0 43 41354.5 44 43173.0 45 45859.0 46 47553.0 47 49127.0 48 51071.0 49 50543.5 50 49325.5 51 48389.0 52 48370.5 53 50109.5 54 51903.0 55 49568.0 56 45327.0 57 41609.0 58 39191.0 59 35416.0 60 28939.5 61 24471.5 62 21192.5 63 16565.5 64 12268.5 65 9643.5 66 7352.5 67 5769.5 68 4559.0 69 3456.0 70 2722.0 71 1992.5 72 1489.0 73 1183.0 74 914.5 75 706.0 76 489.0 77 287.5 78 190.5 79 122.5 80 65.0 81 39.5 82 25.5 83 18.5 84 12.5 85 10.0 86 10.5 87 5.0 88 4.0 89 4.5 90 3.0 91 4.0 92 4.0 93 2.5 94 1.5 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4225395201447975 2 0.03969978990415845 3 0.04852196543841588 4 0.052506173744209565 5 0.0783323811549793 6 0.07904384692387102 7 0.042616799556614536 8 0.03820571178948581 9 0.04311482559483874 10-11 0.0445377571326222 12-13 0.04130058788416484 14-15 0.036355900790367324 16-17 0.03642704736725649 18-19 0.04389743794061964 20-21 0.05225716072509745 22-23 0.043933011229064234 24-25 0.0628935739700288 26-27 0.05321763951310129 28-29 0.04158517419172153 30-31 0.04379071807528588 32-33 0.042510079691280775 34-35 0.04674330101618655 36-37 0.041976480364611976 38-39 0.024011969700095833 40-41 0.023691810104094557 42-43 0.021735279239642302 44-45 0.014300461954723741 46-47 0.02635980673743854 48-49 0.02692897935255192 50-51 0.014798487992947952 52-53 0.015225367454282988 54-55 0.015830113357840958 56-57 0.01462062155072502 58-59 0.013695716051165773 60-61 0.01575896678095178 62-63 0.015509953761839682 64-65 0.01689731201117855 66-67 0.00924905499559247 68-69 0.011276732436933895 70-71 0.01739533804940276 72-73 0.015083074300504642 74-75 0.012166064648048555 76-77 0.009498068014704574 78-79 0.01373128933961036 80-81 0.016577152415177272 82-83 0.014336035243168326 84-85 0.012770810551606527 86-87 0.0034150356906802962 88-89 0.02685783277566275 90-91 0.02963254927434049 92-93 0.01639928597295434 94-95 0.030770894504567253 96-97 0.02475900875743215 98-99 0.021415119643641026 100-101 0.1354275091085405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1405549.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.862658811256075 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43408785325957 48.7486101676553 2 11.067318624166727 13.250382375079056 3 3.1570192539744175 5.6696269938371024 4 1.3665644264910963 3.2722472002654532 5 0.7282960063349931 2.1798867670416047 6 0.4558545575878123 1.6373199509061174 7 0.32124258621902674 1.346130473413258 8 0.2188987562064342 1.0483089245595276 9 0.1641349104554582 0.8842996929250045 >10 0.9801520969866534 10.70435715499379 >50 0.06484498306056281 2.6763841518554283 >100 0.03561101369198959 4.1111473508097784 >500 0.004062953464351297 1.7267847636596136 >1k 0.001672980838262299 1.724996387211066 >5k 2.3899726260889987E-4 1.0195176457880115 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7799 0.554872153158659 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6453 0.45910886066583234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3970 0.28245191025001615 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2137 0.15204023481216236 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2091 0.14876749227526043 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1935 0.13766862628054946 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1742 0.12393733694093909 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.12244325882626646 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1591 0.11319420383067398 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1527 0.10864082290976693 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1466 0.1043008817195274 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 7.114657688917284E-5 6 0.0 0.0 0.0 0.0 7.114657688917284E-5 7 0.0 0.0 0.0 0.0 7.114657688917284E-5 8 0.0 0.0 0.0 0.0 7.114657688917284E-5 9 0.0 0.0 0.0 0.0 7.114657688917284E-5 10-11 0.0 0.0 0.0 7.114657688917284E-5 7.114657688917284E-5 12-13 1.4229315377834568E-4 0.0 0.0 7.114657688917284E-5 7.114657688917284E-5 14-15 2.1343973066751854E-4 0.0 0.0 7.114657688917284E-5 7.114657688917284E-5 16-17 3.2015959600127777E-4 0.0 0.0 7.114657688917284E-5 1.4229315377834568E-4 18-19 4.2687946133503703E-4 0.0 0.0 7.114657688917284E-5 1.4229315377834568E-4 20-21 4.980260382242099E-4 0.0 0.0 1.0671986533375926E-4 1.4229315377834568E-4 22-23 4.980260382242099E-4 0.0 0.0 1.4229315377834568E-4 1.4229315377834568E-4 24-25 4.980260382242099E-4 0.0 0.0 3.557328844458642E-4 1.4229315377834568E-4 26-27 4.980260382242099E-4 0.0 0.0 9.604787880038334E-4 1.4229315377834568E-4 28-29 7.826123457809013E-4 0.0 0.0 0.0023122637488981173 1.4229315377834568E-4 30-31 9.24905499559247E-4 0.0 0.0 0.006936791246694353 1.4229315377834568E-4 32-33 9.24905499559247E-4 0.0 0.0 0.017857790799182385 2.1343973066751854E-4 34-35 9.604787880038334E-4 0.0 0.0 0.036640487097924015 2.1343973066751854E-4 36-37 9.960520764484197E-4 0.0 0.0 0.06677106241048872 2.1343973066751854E-4 38-39 0.0011383452302267654 0.0 0.0 0.11187799215822429 2.1343973066751854E-4 40-41 0.0011383452302267654 0.0 0.0 0.15833670686685417 2.1343973066751854E-4 42-43 0.0012094918071159384 0.0 0.0 0.20728555176660507 2.4901301911210493E-4 44-45 0.0012094918071159384 0.0 0.0 0.26875619419885044 3.2015959600127777E-4 46-47 0.0012806383840051113 0.0 0.0 0.3429620738942577 3.557328844458642E-4 48-49 0.001351784960894284 0.0 0.0 0.4236067187981351 3.557328844458642E-4 50-51 0.001351784960894284 0.0 0.0 0.5135359919860496 3.557328844458642E-4 52-53 0.001351784960894284 0.0 0.0 0.5918327998525843 3.557328844458642E-4 54-55 0.001351784960894284 0.0 0.0 0.6778134380231496 3.557328844458642E-4 56-57 0.001351784960894284 0.0 0.0 0.7792684566671102 4.2687946133503703E-4 58-59 0.001351784960894284 0.0 0.0 0.88894090494177 4.980260382242099E-4 60-61 0.001351784960894284 0.0 0.0 1.0082537143849128 4.980260382242099E-4 62-63 0.001351784960894284 0.0 0.0 1.1286337224813934 5.691726151133827E-4 64-65 0.001351784960894284 0.0 0.0 1.2493694634623198 5.691726151133827E-4 66-67 0.001351784960894284 0.0 0.0 1.3896705130877685 5.691726151133827E-4 68-69 0.001351784960894284 0.0 0.0 1.5435249856106048 5.691726151133827E-4 70-71 0.0014229315377834568 0.0 0.0 1.705810327494808 5.691726151133827E-4 72-73 0.0014229315377834568 0.0 0.0 1.871830864665693 5.691726151133827E-4 74-75 0.0014229315377834568 0.0 0.0 2.032266395550778 6.047459035579692E-4 76-77 0.0014229315377834568 0.0 0.0 2.2128719809839428 7.114657688917284E-4 78-79 0.0014229315377834568 0.0 0.0 2.414857112772305 7.114657688917284E-4 80-81 0.0017430911337847346 0.0 0.0 2.6390399765500883 7.470390573363149E-4 82-83 0.001778664422229321 0.0 0.0 2.8638987328083187 7.826123457809012E-4 84-85 0.001778664422229321 0.0 0.0 3.0929551371030106 7.826123457809012E-4 86-87 0.001849810999118494 0.0 0.0 3.3245728181657133 8.181856342254877E-4 88-89 0.0019209575760076667 0.0 0.0 3.5764672736418297 8.537589226700742E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3965 0.0 46.684826 1 GTATCAA 6375 0.0 36.81901 1 CGCGTAA 30 0.003952452 31.665789 10-11 TATCAAC 7970 0.0 29.464964 2 ATCAACG 7955 0.0 29.341248 3 TCAACGC 8115 0.0 28.762741 4 CAACGCA 8195 0.0 28.423946 5 AACGCAG 8320 0.0 28.048046 6 ACGCAGA 9500 0.0 24.501066 7 CGCAGAG 9510 0.0 24.3754 8 GAGTACT 6275 0.0 22.558748 12-13 TAAGGTG 865 0.0 22.532274 5 GTGGTAT 2160 0.0 22.528572 1 TACATGG 4980 0.0 22.24149 2 GCAGAGT 10230 0.0 22.1947 9 GTACATG 5040 0.0 21.865965 1 TGGTATC 2180 0.0 21.588179 2 GTACTTT 6510 0.0 21.346134 14-15 GTAAGGT 830 0.0 21.19146 4 AGGTAAG 875 0.0 21.18819 2 >>END_MODULE