FastQCFastQC Report
Thu 26 May 2016
SRR937922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405549
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78690.5598524135409011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68870.4899864750357334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.30528996143144066No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18710.1331152453596424No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18570.13211919328319396No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18340.13048282201474298No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17550.12486224244049834No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16600.11810331763602692No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16180.11511516140668165No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14690.1045143214501949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42450.048.1259961
GTATCAA68550.036.3172341
TAAGGTG10250.030.5833745
TATCAAC86850.028.6101952
GTAAGGT10250.028.266454
TCAACGC89300.028.0300884
ATCAACG88350.027.9581493
GGACCGA4250.027.9393566
CAACGCA90500.027.6059365
GTACGCT700.00839049327.1439863
AACGCAG93350.026.712246
AAGGTAA10900.024.4090251
GGTAAGG11700.024.3599853
AGGTAAG11400.023.7552132
ACGCAGA106700.023.1475097
AGGACCG5350.023.082615
CGCAGAG106600.023.0355538
GAGTACT74350.022.32705912-13
GGACCGT1503.03536E-522.1652226
GTGTAAG6900.022.0338211
Produced by FastQC (version 0.11.5)