FastQCFastQC Report
Thu 26 May 2016
SRR937921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416244
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79200.5592256701528833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64320.45415902909385675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41310.29168702568201527No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22160.15647021275994813No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21530.1520218267473684No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20210.142701398911487No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17820.1258257757843987No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.12053007815037522No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA16430.11601108283600847No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14980.10577273407689634No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14630.10330140851435206No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14220.10040642714108587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35750.046.9615551
GTATCAA60350.040.7800941
TATCAAC76400.031.7825362
ATCAACG77100.031.3707123
TCAACGC77650.031.148514
CAACGCA79200.030.7189085
AACGCAG81250.029.885366
CGCAGAG92200.026.2487668
ACGCAGA93200.026.0190647
GCAGAGT99700.024.177959
TAAGGTG9450.023.6334445
GAGTACT62300.023.19981612-13
GTAAGGT9350.022.8697744
AGAGTAC86300.022.2024510-11
AGTACTT63100.021.96383512-13
GTACTTT66000.021.81098414-15
GTGTAAG6000.021.4628961
GTACATG51350.021.3630491
CAGAGTA95700.020.8165510-11
TACATGG49600.020.6934572
Produced by FastQC (version 0.11.5)