FastQCFastQC Report
Thu 26 May 2016
SRR937921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416244
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80240.5665690375387291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71000.5013260426875595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45110.31851856036106774No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18820.13288670596309676No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18760.1324630501523749No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.13027416179697848No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17690.124907854861168No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17580.12413115254151122No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16810.11869423630391372No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14620.10323079921256506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.051.9623951
GTATCAA62950.036.6785051
TAAGGTG9800.031.0193715
TACGCTC804.4950427E-429.6865064
TATCAAC78800.028.999462
ATCAACG79850.028.6120683
TCAACGC81450.028.108334
GTAAGGT10000.028.0240634
CAACGCA82750.027.666755
GTACCGT700.00839358727.1419496
AACGCAG85500.026.8324386
GGACCGA4750.024.9991636
AGGTAAG9500.024.504372
ACCGAGT5100.024.2148768
ACGCAGA95850.023.885487
CGCAGAG96300.023.7738678
GGTAAGG11050.023.6417433
AAGGTAA9950.022.9186611
GAGTACT68000.022.03786512-13
GCAGAGT103950.021.978589