##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937921_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1416244 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.911609863837022 31.0 31.0 34.0 30.0 34.0 2 32.19180663783924 33.0 31.0 34.0 30.0 34.0 3 32.24509547789788 34.0 31.0 34.0 30.0 34.0 4 35.88718398806985 37.0 35.0 37.0 35.0 37.0 5 35.81097325037211 37.0 35.0 37.0 35.0 37.0 6 35.87458234597993 37.0 35.0 37.0 35.0 37.0 7 35.82098706155154 37.0 35.0 37.0 35.0 37.0 8 35.81239955826821 37.0 35.0 37.0 35.0 37.0 9 37.39903434718876 39.0 37.0 39.0 34.0 39.0 10-11 37.35116441799577 39.0 37.0 39.0 34.0 39.0 12-13 37.28549564905482 39.0 37.0 39.0 34.0 39.0 14-15 38.36476023905485 40.0 38.0 41.0 33.5 41.0 16-17 38.254024377155346 40.0 38.0 41.0 33.0 41.0 18-19 38.2801367560957 40.0 38.0 41.0 33.5 41.0 20-21 38.172570898799925 40.0 38.0 41.0 33.0 41.0 22-23 37.96834514391588 40.0 37.0 41.0 33.0 41.0 24-25 37.882447516105984 40.0 37.0 41.0 33.0 41.0 26-27 37.794412897777505 40.0 37.0 41.0 33.0 41.0 28-29 37.706093017869804 39.5 37.0 41.0 32.5 41.0 30-31 37.41100332993467 39.0 36.5 40.5 32.0 41.0 32-33 37.14430069959696 39.0 36.0 40.0 31.5 41.0 34-35 36.85700874990468 39.0 35.0 40.0 31.0 41.0 36-37 36.65395016677917 39.0 35.0 40.0 30.5 41.0 38-39 36.59865531645677 39.0 35.0 40.0 30.0 41.0 40-41 36.481913427347266 39.0 35.0 40.0 30.0 41.0 42-43 36.23992758310008 39.0 35.0 40.0 30.0 41.0 44-45 35.93407350710753 38.0 35.0 40.0 29.5 41.0 46-47 35.46871902016884 38.0 35.0 40.0 27.5 41.0 48-49 34.968625109797465 38.0 34.0 40.0 26.0 41.0 50-51 34.52922836742821 38.0 33.5 40.0 24.5 41.0 52-53 33.92033223088677 37.5 33.0 40.0 23.5 41.0 54-55 33.56139831836887 37.0 33.0 40.0 22.5 41.0 56-57 33.21161537136256 37.0 32.0 39.0 22.0 40.5 58-59 32.74799081231765 36.0 31.5 39.0 19.5 40.0 60-61 32.23583224359644 35.5 31.0 39.0 18.0 40.0 62-63 31.732934437851107 35.0 30.5 38.5 15.5 40.0 64-65 31.211757649105664 35.0 29.5 38.0 12.0 40.0 66-67 30.66627113689449 34.0 29.0 37.5 8.0 40.0 68-69 30.082427533673577 34.0 28.5 37.0 7.5 39.0 70-71 29.410798916006 34.0 27.5 36.0 6.5 39.0 72-73 28.88026745391331 33.0 26.5 36.0 2.0 38.5 74-75 28.329350733348207 33.0 26.0 35.0 2.0 38.0 76-77 27.3575076046218 31.5 25.5 34.0 2.0 36.0 78-79 28.15979379259506 33.0 26.0 35.0 2.0 37.0 80-81 27.960187651280428 33.0 26.0 35.0 2.0 36.5 82-83 27.45463140532281 33.0 26.0 35.0 2.0 36.0 84-85 26.896267874744748 32.0 25.0 35.0 2.0 35.5 86-87 26.239904282030498 32.0 24.0 34.0 2.0 35.0 88-89 25.463676103835216 31.0 21.0 34.0 2.0 35.0 90-91 24.833386407991846 31.0 19.5 34.0 2.0 35.0 92-93 24.10214871166268 31.0 16.5 34.0 2.0 35.0 94-95 23.254784839335592 30.0 4.5 34.0 2.0 35.0 96-97 22.181887796170717 29.5 2.0 34.0 2.0 35.0 98-99 20.95168876267084 29.0 2.0 34.0 2.0 35.0 100-101 18.412813399385982 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2580088299247869 1101 2 0.2688719652073672 1101 3 0.2201061595303173 1101 4 0.14805589733208535 1101 5 0.1546487612122931 1101 6 0.11261088326704538 1101 7 0.1909780926435971 1101 8 0.1382765502093264 1101 9 0.19872205059767367 1101 10-11 0.18806224726451148 1101 12-13 0.26022008879099445 1101 14-15 0.3152368309352198 1101 16-17 0.4101661051917631 1101 18-19 0.37050634911700087 1101 20-21 0.41406010750329614 1101 22-23 0.3307672222561351 1101 24-25 0.420553766961973 1101 26-27 0.35228202667225617 1101 28-29 0.4946447013600377 1101 30-31 0.5051825824445615 1101 32-33 0.5952985472314154 1101 34-35 0.4816734083157357 1101 36-37 0.3849829748243465 1101 38-39 0.6401277102383318 1101 40-41 0.6134623240844803 1101 42-43 0.895129801797161 1101 44-45 0.9879285354920171 1101 46-47 0.8648175218975425 1101 48-49 0.9538399043294774 1101 50-51 0.95535067653487 1101 52-53 0.8038433543776122 1101 54-55 1.1792401187983828 1101 56-57 1.050106711591276 1101 58-59 1.0020084078078852 1101 60-61 1.0900239101338656 1101 62-63 1.0742396663437113 1101 64-65 1.0927671911524364 1101 66-67 1.0342810446976642 1101 68-69 1.0532120434347512 1101 70-71 1.0807353794956498 1101 72-73 1.1010516771007133 1101 74-75 1.162136713188179 1101 76-77 1.194150476005131 1101 78-79 1.0488926920799457 1101 80-81 1.2833215599451258 1101 82-83 1.4837128786537157 1101 84-85 1.520689934114447 1101 86-87 1.7054859330070258 1101 88-89 1.2475949236552317 1101 90-91 1.6169925408575772 1101 92-93 1.9422537920218517 1101 94-95 1.6175100646502152 1101 96-97 1.895238323893789 1101 98-99 1.9315983566434625 1101 100-101 2.0878447875851442 1102 1 0.16275325768664217 1102 2 0.16119645676749883 1102 3 0.12784081533813918 1102 4 0.021817720486509984 1102 5 0.06611677866329302 1102 6 0.1252699366058394 1102 7 0.035516060704019026 1102 8 0.1431589884963742 1102 9 0.10198071669814368 1102 10-11 0.16572173030640158 1102 12-13 0.19836988834713765 1102 14-15 0.2780222675388515 1102 16-17 0.2256596823942445 1102 18-19 0.22066609756375755 1102 20-21 0.2565673492643725 1102 22-23 0.32622854644943544 1102 24-25 0.08350451287600436 1102 26-27 0.05394093340645867 1102 28-29 0.12409604633074167 1102 30-31 0.15925566230361454 1102 32-33 0.04264247653767228 1102 34-35 0.3194910726518927 1102 36-37 0.17914609012127158 1102 38-39 0.332380163337497 1102 40-41 0.5479636915514092 1102 42-43 0.5154833210655525 1102 44-45 0.31765337384781844 1102 46-47 0.3584905228934403 1102 48-49 0.511181760950187 1102 50-51 0.7200381812742407 1102 52-53 0.4262579319289088 1102 54-55 0.557114200623964 1102 56-57 0.7854219872837334 1102 58-59 0.8283840896455956 1102 60-61 0.8429132843345002 1102 62-63 0.6922192023654681 1102 64-65 0.6600698184077451 1102 66-67 0.7158043169120489 1102 68-69 1.0000236464295327 1102 70-71 1.1493642727321252 1102 72-73 1.0978549388000118 1102 74-75 1.357088965590723 1102 76-77 1.2905986007302452 1102 78-79 1.2274730035894166 1102 80-81 1.3604742460950376 1102 82-83 1.593219680601031 1102 84-85 1.350833353843381 1102 86-87 1.1667843441166177 1102 88-89 1.1005191796369544 1102 90-91 1.1557429136882114 1102 92-93 1.2107171231813894 1102 94-95 1.3266718402845292 1102 96-97 1.5356172714750684 1102 98-99 1.5570036677737846 1102 100-101 1.214524547558046 1103 1 0.13324471781313107 1103 2 0.13439117186415928 1103 3 0.11733629745349106 1103 4 0.17358624929581623 1103 5 0.14574518078243415 1103 6 0.04190910888352306 1103 7 0.10519215852756503 1103 8 0.05121143881957835 1103 9 -0.061581659016418655 1103 10-11 -0.04658445917064569 1103 12-13 0.06548751283192189 1103 14-15 0.19635820079476218 1103 16-17 0.19667395123454412 1103 18-19 0.1401493193413046 1103 20-21 0.31722512137160663 1103 22-23 0.21453686902589908 1103 24-25 0.2202244738024035 1103 26-27 0.2056249854979484 1103 28-29 0.4135182654518914 1103 30-31 0.2622731507285678 1103 32-33 0.2713678001158968 1103 34-35 0.24537010398552894 1103 36-37 0.3000380047773632 1103 38-39 0.412835639870913 1103 40-41 0.2745917996217955 1103 42-43 0.29272469522016564 1103 44-45 0.20230851446790865 1103 46-47 0.25249815819324795 1103 48-49 0.364539398991127 1103 50-51 0.3220287418109109 1103 52-53 0.3669460558480324 1103 54-55 0.4740322208286045 1103 56-57 0.664948967502724 1103 58-59 0.5826510360738979 1103 60-61 0.46886682463163254 1103 62-63 0.5528508091176327 1103 64-65 0.5103337147032292 1103 66-67 0.37244358886727014 1103 68-69 0.5118870184211382 1103 70-71 0.5859686077084767 1103 72-73 0.5851303528356091 1103 74-75 0.6023839363602832 1103 76-77 0.4004577065230066 1103 78-79 0.4834152803241807 1103 80-81 0.6540866599362616 1103 82-83 0.732863682255676 1103 84-85 0.8096396155866437 1103 86-87 1.0471643149111003 1103 88-89 1.0027933227838766 1103 90-91 0.7408966912179018 1103 92-93 0.9297824468592921 1103 94-95 0.9427313876488093 1103 96-97 0.8816309173307282 1103 98-99 1.0086453569094331 1103 100-101 1.1059470457763076 1104 1 0.1986754818718275 1104 2 0.1643832146829851 1104 3 0.19198578241345388 1104 4 0.05367805920383262 1104 5 0.12064789275424914 1104 6 0.024902508534758283 1104 7 0.18462804517280063 1104 8 0.1257162917326582 1104 9 0.12912029013018156 1104 10-11 0.27096245554724163 1104 12-13 0.20207240676439397 1104 14-15 0.35818428922347323 1104 16-17 0.33771462476519076 1104 18-19 0.31170953150055425 1104 20-21 0.37253309330621676 1104 22-23 0.3031576162269758 1104 24-25 0.3248401226029074 1104 26-27 0.30668650732698666 1104 28-29 0.26797126366454194 1104 30-31 0.3027420681407662 1104 32-33 0.3822974358995097 1104 34-35 0.46331729635824814 1104 36-37 0.45517425638875153 1104 38-39 0.503652472332341 1104 40-41 0.5496321297117959 1104 42-43 0.5504476353623318 1104 44-45 0.6128069145665975 1104 46-47 0.5089822934121102 1104 48-49 0.4423828819629989 1104 50-51 0.5186656839314807 1104 52-53 0.7610357325542978 1104 54-55 0.6185157659100184 1104 56-57 0.6557575767374075 1104 58-59 0.6766906478408572 1104 60-61 0.698002684430449 1104 62-63 0.7442086815270095 1104 64-65 1.0751264531102898 1104 66-67 0.8797978140337328 1104 68-69 1.0167184059939096 1104 70-71 1.0691245370177462 1104 72-73 1.0171402303820862 1104 74-75 0.8304011954620627 1104 76-77 0.8806334417157551 1104 78-79 0.9163144756572557 1104 80-81 1.0365300708339689 1104 82-83 0.9985038967367785 1104 84-85 1.2805465649388204 1104 86-87 1.289823512953003 1104 88-89 1.2575188967771957 1104 90-91 1.252667031006471 1104 92-93 1.3465762107511026 1104 94-95 1.326867332098491 1104 96-97 1.646682687380281 1104 98-99 1.6391075748104278 1104 100-101 1.5545651808936007 1105 1 0.08689241585870278 1105 2 0.05204407923984178 1105 3 0.11637751931213813 1105 4 0.05611165411584551 1105 5 0.07300314064818991 1105 6 0.10913934166962491 1105 7 0.07849247454158359 1105 8 0.09321516377068662 1105 9 0.022128915083499123 1105 10-11 0.10873643276376299 1105 12-13 0.190387037177139 1105 14-15 0.22633728878857085 1105 16-17 0.3663293699181125 1105 18-19 0.15580461666055356 1105 20-21 0.26047493115636655 1105 22-23 0.2772847906618878 1105 24-25 0.26205152030827605 1105 26-27 0.242016310749662 1105 28-29 0.25650154051478324 1105 30-31 0.43492813530129126 1105 32-33 0.27171602236197145 1105 34-35 0.32127275112809883 1105 36-37 0.5120484047661549 1105 38-39 0.5031040884147799 1105 40-41 0.3925999310313486 1105 42-43 0.3153582076436763 1105 44-45 0.4235419027263774 1105 46-47 0.49939823334576516 1105 48-49 0.6620841054061657 1105 50-51 0.6908885240086278 1105 52-53 0.6697465375710365 1105 54-55 0.7466465950321464 1105 56-57 0.7933601218174573 1105 58-59 0.7612061938241794 1105 60-61 0.5637347475746282 1105 62-63 0.6183095895929149 1105 64-65 0.6426000533750376 1105 66-67 0.6153246378284436 1105 68-69 0.32190377603324904 1105 70-71 0.4367194327524686 1105 72-73 0.5920222125571222 1105 74-75 0.892454701095545 1105 76-77 0.5614570483549564 1105 78-79 0.6009430890913521 1105 80-81 0.6142588572373029 1105 82-83 0.7500358002031255 1105 84-85 0.8186355125390001 1105 86-87 0.856609259853709 1105 88-89 0.8039714902759734 1105 90-91 0.8223459212546409 1105 92-93 0.9489748139770313 1105 94-95 0.8516544660081493 1105 96-97 0.9299383518289979 1105 98-99 0.8625943652228898 1105 100-101 0.4555346404754914 1106 1 -0.08852083095938568 1106 2 -0.056437707873307374 1106 3 -0.05920051986313268 1106 4 0.01824894436093416 1106 5 -0.005508202485032143 1106 6 -0.0395060296195453 1106 7 -0.06307748581053119 1106 8 -0.15581007389010182 1106 9 -0.20122202867477768 1106 10-11 -0.11874031641160343 1106 12-13 -0.2578124175422758 1106 14-15 -0.3926185850595516 1106 16-17 -0.18635066017980506 1106 18-19 -0.1654007839092415 1106 20-21 -0.14969169123993709 1106 22-23 -0.18015636158759918 1106 24-25 -0.19359779267193744 1106 26-27 -0.3603546115278675 1106 28-29 -0.18844337619622564 1106 30-31 -0.0550712783755003 1106 32-33 -0.07260006270463748 1106 34-35 -0.10332874897095223 1106 36-37 -0.25523680327642495 1106 38-39 -0.25768270403302296 1106 40-41 -0.2856689763375684 1106 42-43 -0.47296356653482974 1106 44-45 -0.2533631368992957 1106 46-47 -0.3627458529183727 1106 48-49 -0.35198187727598196 1106 50-51 -0.49804036120438866 1106 52-53 -0.4608940471886527 1106 54-55 -0.7936571292751182 1106 56-57 -0.6101432926268799 1106 58-59 -0.3811772899839667 1106 60-61 -0.31455676877268957 1106 62-63 -0.3513286401018725 1106 64-65 -0.30855056150922167 1106 66-67 -0.40714464460966937 1106 68-69 -0.37226515079094824 1106 70-71 -0.7718113034651601 1106 72-73 -0.7571405889662515 1106 74-75 -0.49168361255005877 1106 76-77 -0.30278714104876414 1106 78-79 -0.5456550369841295 1106 80-81 -0.789570144263152 1106 82-83 -0.5817729447187396 1106 84-85 -0.25397484281728566 1106 86-87 -0.30158562865160476 1106 88-89 -0.04575653570702798 1106 90-91 -0.5021788682242381 1106 92-93 -0.25699031400577965 1106 94-95 -0.44646734530416055 1106 96-97 -0.2088441302548283 1106 98-99 -0.1522624875184846 1106 100-101 -0.18449710981478162 1107 1 0.10031523178886559 1107 2 0.1161699097709814 1107 3 0.12421911481884962 1107 4 0.09787252084453968 1107 5 0.0811116659421316 1107 6 -0.004452501365427963 1107 7 -0.001391594355297343 1107 8 0.06415265017901106 1107 9 0.001214582488984206 1107 10-11 0.09290847711334038 1107 12-13 0.13042100463217565 1107 14-15 0.23480292861415109 1107 16-17 0.2247860125895258 1107 18-19 0.11834042405493506 1107 20-21 0.08380472248208548 1107 22-23 0.12819277834388743 1107 24-25 0.0552937194344878 1107 26-27 0.1475681518403107 1107 28-29 0.1392134021752227 1107 30-31 0.1326069121659046 1107 32-33 0.060897349557343716 1107 34-35 0.2065006179156441 1107 36-37 0.11273125039119947 1107 38-39 0.09241256970368283 1107 40-41 0.18236884488296567 1107 42-43 0.1854107322621914 1107 44-45 0.2547955858031159 1107 46-47 0.4262277044410254 1107 48-49 0.3349092032875234 1107 50-51 0.2454351569835822 1107 52-53 0.13646794047894417 1107 54-55 0.2684314342730474 1107 56-57 0.3449187188754266 1107 58-59 0.16694821172809782 1107 60-61 0.2978566892884231 1107 62-63 0.6228243700836664 1107 64-65 0.53640570461123 1107 66-67 0.40109834618248996 1107 68-69 0.3757383119719755 1107 70-71 0.41121868355816105 1107 72-73 0.5176296057446983 1107 74-75 0.3623391793416211 1107 76-77 0.23560975948320007 1107 78-79 0.4453038348914724 1107 80-81 0.537047284253326 1107 82-83 0.33969905079391083 1107 84-85 0.6584247659287108 1107 86-87 0.7242120853865224 1107 88-89 0.9484755935752425 1107 90-91 0.9109548844669355 1107 92-93 1.0572753922680462 1107 94-95 0.9317120819072713 1107 96-97 1.01521282472795 1107 98-99 1.215372140631743 1107 100-101 1.2859617366631717 1108 1 -0.03237218149771337 1108 2 -0.044550724461068114 1108 3 -0.009642231841880289 1108 4 -0.04345168798040788 1108 5 -0.09366921622773106 1108 6 0.02431057757431887 1108 7 0.04637474498760241 1108 8 -0.10918776782511941 1108 9 0.010332673598021813 1108 10-11 0.05595952310765995 1108 12-13 0.018692195220516794 1108 14-15 0.0536956835542739 1108 16-17 -0.0659577965590259 1108 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44-45 -0.5223150706072488 2316 46-47 -0.7370316974694475 2316 48-49 -0.6759505093756459 2316 50-51 -0.5786168831516605 2316 52-53 -0.4058778047257192 2316 54-55 -0.581761035444984 2316 56-57 -0.6583630047078586 2316 58-59 -0.49633920169335966 2316 60-61 -0.23387378745437104 2316 62-63 -0.42546266036790925 2316 64-65 -0.6133843184763812 2316 66-67 -0.3663969214040321 2316 68-69 -0.32875381526156033 2316 70-71 -0.5656238939385254 2316 72-73 -0.3864837095708147 2316 74-75 -0.17020275585987577 2316 76-77 -0.08740957502457647 2316 78-79 -0.1511765625218402 2316 80-81 -0.348183208902455 2316 82-83 -0.2598847156601174 2316 84-85 0.01964261013656099 2316 86-87 0.06880772390672618 2316 88-89 -0.02194884646808859 2316 90-91 -0.2838227194546725 2316 92-93 -0.39712777653413056 2316 94-95 -0.10028472302975544 2316 96-97 -0.10714670739227827 2316 98-99 0.18860582885049837 2316 100-101 0.312901588508538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 22.0 5 42.0 6 102.0 7 230.0 8 634.0 9 1129.0 10 1774.0 11 2970.0 12 4236.0 13 6249.0 14 8417.0 15 11247.0 16 14139.0 17 18785.0 18 18383.0 19 14865.0 20 13882.0 21 14007.0 22 14547.0 23 15896.0 24 18131.0 25 21263.0 26 25433.0 27 30330.0 28 36528.0 29 44315.0 30 53312.0 31 65200.0 32 80427.0 33 102701.0 34 133677.0 35 177056.0 36 209833.0 37 187303.0 38 66756.0 39 2411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.850455147559316 19.415792758874883 15.40278370111365 22.330968392452146 2 16.919222235247428 20.592729958801122 38.64297931299424 23.845068492957207 3 20.336926871602152 23.709867507253808 30.157193467090064 25.796012154053976 4 14.074481515896977 17.23389472435541 35.498261598990005 33.19336216075761 5 13.969344265536165 37.03126015008713 33.87262364394836 15.126771940428343 6 33.1331558214233 35.940866080185394 16.13543720957491 14.790540888816398 7 29.01336210427017 32.96303461832848 19.573816376274145 18.449786901127208 8 26.966031911235444 34.88700016452002 18.687214958471053 19.45975296577348 9 26.40631079032453 16.651615085285627 19.629215649031487 37.31285847535836 10-11 25.73500752695157 26.281382304179225 26.162652763224415 21.82095740564479 12-13 26.646467699068804 23.553603757544604 27.021544310161243 22.77838423322535 14-15 22.989258913012165 25.0618184437145 25.71071792713685 26.238204716136487 16-17 22.28675930633226 28.226783596000676 26.68025899565045 22.806198102016605 18-19 22.248673251219422 26.11746281008075 29.653223597063782 21.980640341636047 20-21 22.597306678792638 25.228880051742497 30.235679727504582 21.938133541960283 22-23 22.454358147324896 25.194669845026706 30.16849497685427 22.18247703079413 24-25 22.461083277725404 25.43951446335702 30.13748397962102 21.96191827929655 26-27 21.80188305137235 25.875447977730797 30.064624616819913 22.258044354076937 28-29 21.94018895761922 26.241108961841658 29.915406496128664 21.903295584410447 30-31 22.14558367061043 25.722227243328128 29.80309184010665 22.32909724595479 32-33 21.820280849786847 25.768771127723987 29.84898366269782 22.561964359791347 34-35 22.70126449079616 25.875768457999204 29.501830880976847 21.921136170227793 36-37 22.827509945727467 25.47906842745233 29.535527112963976 22.15789451385623 38-39 22.166242937853106 25.84343220338983 29.450494350282487 22.539830508474576 40-41 22.62699906923139 25.68405843362518 29.481848599680514 22.207093897462915 42-43 22.3913301377563 25.747109716380766 29.255877310179052 22.60568283568388 44-45 22.39417340743322 25.998969775733805 29.403864506090788 22.20299231074219 46-47 22.90781917092647 26.419574035114724 28.54226691949513 22.13033987446368 48-49 22.70950485933215 26.4355577146311 28.37254738590243 22.48239004013433 50-51 22.812559135290247 26.66097791058603 28.36732229757019 22.15914065655353 52-53 23.213902406647442 26.410632631100288 28.190551910546485 22.184913051705777 54-55 22.616229971671547 26.626449962012195 28.34772821632431 22.40959184999195 56-57 22.749257896238216 26.819707621003158 28.02161915602114 22.409415326737484 58-59 22.789681721511265 26.330067417761345 28.062254809199544 22.817996051527846 60-61 22.45972445426071 26.621507310887104 28.462644854982617 22.45612337986957 62-63 22.435505463747774 26.720819293850496 28.520085521986328 22.32358972041541 64-65 22.08669551292009 26.71601786132898 28.41141780653616 22.78586881921477 66-67 22.12150589870107 26.74366140297858 28.207992408087872 22.926840290232477 68-69 22.644203917271142 26.361403179261156 28.404522959016155 22.589869944451543 70-71 22.531216372320024 26.57088044150584 27.975370063350663 22.92253312282347 72-73 22.472081082073426 26.69977772191797 27.974169705220287 22.85397149078831 74-75 22.422407438266287 27.114854502472735 27.691061709705387 22.771676349555587 76-77 22.562884644171486 26.654481854821626 27.993657872513495 22.788975628493397 78-79 22.527085728165485 26.900061006436744 27.959800712306638 22.613052553091133 80-81 22.705021168668676 27.017978540421 27.653356342551145 22.62364394835918 82-83 22.639080073447822 27.042065859437308 27.731106974189114 22.58774709292576 84-85 22.642365433622583 27.142163131259284 27.58006124854099 22.63541018657715 86-87 22.808937631532586 27.025818074876618 27.32996925301671 22.835275040574086 88-89 22.537320599163138 27.312493203845108 27.158670622676066 22.991515574315688 90-91 22.560417838341838 27.244788406497054 27.260040042605727 22.93475371255538 92-93 22.677630933240696 26.760987038186407 27.482370535440044 23.07901149313286 94-95 22.519601142176064 26.580659829803338 27.68308285860346 23.216656169417135 96-97 22.68073957620988 26.321215243000236 27.631653737510536 23.36639144327935 98-99 22.44337840089702 26.657376836194892 27.24103332476607 23.658211438142015 100-101 22.701208266372177 26.244241811439274 27.44756553249299 23.606984389695562 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 715.0 1 505.5 2 442.0 3 591.5 4 459.0 5 394.5 6 851.0 7 1883.0 8 3022.5 9 3477.5 10 2794.5 11 1940.5 12 1753.5 13 1854.5 14 1979.0 15 2099.5 16 2269.0 17 2417.0 18 2564.0 19 2812.0 20 3122.5 21 3571.5 22 4243.5 23 5019.0 24 6006.0 25 7419.5 26 9239.5 27 11286.5 28 13147.0 29 14686.5 30 16363.0 31 19279.0 32 23372.5 33 24842.0 34 24511.5 35 25784.5 36 27982.5 37 30212.5 38 32265.5 39 33914.5 40 35924.0 41 38670.0 42 40572.0 43 42306.0 44 44141.0 45 46731.0 46 48117.0 47 49668.5 48 51645.0 49 50883.0 50 49756.0 51 48671.5 52 48340.0 53 49907.0 54 51630.0 55 49398.5 56 45117.5 57 41352.0 58 38345.0 59 34306.5 60 28275.0 61 24102.5 62 20843.0 63 16043.5 64 11802.0 65 9199.5 66 6971.0 67 5641.5 68 4426.0 69 3236.5 70 2563.0 71 1854.0 72 1369.5 73 1088.0 74 821.5 75 629.5 76 452.5 77 277.5 78 171.5 79 111.0 80 60.0 81 35.5 82 24.0 83 10.0 84 3.0 85 5.0 86 5.5 87 2.5 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01313333013237832 3 0.006425446462615199 4 0.0 5 0.0 6 7.060930178698021E-5 7 0.0 8 2.118279053609406E-4 9 2.118279053609406E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8243720714792085E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0055781348411714365 26-27 0.021006267281626613 28-29 3.5304650893490107E-5 30-31 0.0 32-33 4.5896046161537137E-4 34-35 0.018287809162827875 36-37 0.0040953395036448514 38-39 0.017228669636023174 40-41 0.014827953375265844 42-43 0.008473116214437625 44-45 0.0033539418348815598 46-47 3.5304650893490107E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.118279053609406E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5304650893490107E-5 84-85 0.00331863718398807 86-87 5.295697634023516E-4 88-89 1.4121860357396043E-4 90-91 1.7652325446745052E-4 92-93 4.942651125088614E-4 94-95 0.0 96-97 3.5304650893490107E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1416244.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.829233269919115 #Duplication Level Percentage of deduplicated Percentage of total 1 81.49956135471682 48.76056267687438 2 10.983342496337126 13.142499205935392 3 3.182196736613848 5.711651725969357 4 1.3264311217527656 3.1743742799930676 5 0.7444993742690992 2.2271413366227377 6 0.4707230623498474 1.6897799941715819 7 0.3035201659513579 1.2711565167579848 8 0.2216967741506409 1.0611158412677815 9 0.17538005091822634 0.944356857954917 >10 0.9851864585060419 10.728197222934845 >50 0.06581764311450801 2.7080448480305543 >100 0.03527423960127449 3.97768326298923 >500 0.00471890497668606 1.9993090364313688 >1k 0.0014156714930058177 1.5366432617435337 >5k 2.3594524883430298E-4 1.0674839323232375 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8024 0.5665690375387291 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7100 0.5013260426875595 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4511 0.31851856036106774 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1882 0.13288670596309676 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1876 0.1324630501523749 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1845 0.13027416179697848 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1769 0.124907854861168 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1758 0.12413115254151122 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1681 0.11869423630391372 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1462 0.10323079921256506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.060930178698021E-5 0.0 0.0 0.0 0.0 6 1.4121860357396043E-4 0.0 0.0 0.0 0.0 7 2.1182790536094064E-4 0.0 0.0 0.0 0.0 8 2.1182790536094064E-4 0.0 0.0 0.0 0.0 9 2.1182790536094064E-4 0.0 0.0 0.0 0.0 10-11 2.1182790536094064E-4 0.0 0.0 0.0 0.0 12-13 2.4713255625443076E-4 0.0 0.0 0.0 0.0 14-15 2.8243720714792085E-4 0.0 0.0 0.0 0.0 16-17 3.1774185804141095E-4 0.0 0.0 0.0 0.0 18-19 3.5304650893490104E-4 0.0 0.0 0.0 0.0 20-21 3.5304650893490104E-4 0.0 0.0 0.0 0.0 22-23 3.5304650893490104E-4 0.0 0.0 0.0 3.5304650893490107E-5 24-25 5.648744142958417E-4 0.0 0.0 1.4121860357396043E-4 7.060930178698021E-5 26-27 5.648744142958417E-4 0.0 0.0 3.5304650893490104E-4 7.060930178698021E-5 28-29 5.648744142958417E-4 0.0 0.0 0.0017299278937810152 7.060930178698021E-5 30-31 5.648744142958417E-4 0.0 0.0 0.0067431883206566096 7.060930178698021E-5 32-33 5.648744142958417E-4 0.0 0.0 0.018252504511934383 7.060930178698021E-5 34-35 6.001790651893317E-4 0.0 0.0 0.0356930020533185 7.060930178698021E-5 36-37 6.354837160828219E-4 0.0 0.0 0.06404263672079105 7.060930178698021E-5 38-39 7.060930178698021E-4 0.0 0.0 0.10905606660999093 7.060930178698021E-5 40-41 7.413976687632921E-4 0.0 0.0 0.15537576858224994 7.060930178698021E-5 42-43 0.0010944441776981934 0.0 0.0 0.2054730682001124 7.060930178698021E-5 44-45 0.0014121860357396042 0.0 0.0 0.2684565653940988 7.060930178698021E-5 46-47 0.0016240139411005448 0.0 0.0 0.3472212415374752 7.060930178698021E-5 48-49 0.0016946232428875251 0.0 0.0 0.4228791084022245 7.060930178698021E-5 50-51 0.0016946232428875251 0.0 0.0 0.5036561496465298 7.060930178698021E-5 52-53 0.0016946232428875251 0.0 0.0 0.5785373141916224 1.4121860357396043E-4 54-55 0.0017652325446745052 0.0 0.0 0.6626682972708092 1.7652325446745052E-4 56-57 0.0018358418464614855 0.0 0.0 0.7607093128020312 2.1182790536094064E-4 58-59 0.0018358418464614855 0.0 0.0 0.8681060608200282 2.1182790536094064E-4 60-61 0.0018358418464614855 0.0 0.0 0.9878594366507466 2.1182790536094064E-4 62-63 0.0018358418464614855 0.0 0.0 1.1051767915698143 2.1182790536094064E-4 64-65 0.0018358418464614855 0.0 0.0 1.2232002395067516 2.1182790536094064E-4 66-67 0.0019064511482484656 0.0 0.0 1.3573932175529075 2.1182790536094064E-4 68-69 0.0019064511482484656 0.0 0.0 1.5117098466083527 2.1182790536094064E-4 70-71 0.0020123651009289357 0.0 0.0 1.6683918872736618 2.1182790536094064E-4 72-73 0.0021182790536094062 0.0 0.0 1.8309344999872903 2.8243720714792085E-4 74-75 0.0021182790536094062 0.0 0.0 1.9908292638839071 3.883511598283912E-4 76-77 0.0021182790536094062 0.0 0.0 2.163398397451287 4.942651125088615E-4 78-79 0.0021535837045028963 0.0 0.0 2.3594451238628373 4.942651125088615E-4 80-81 0.0024713255625443075 0.0 0.0 2.570425717602334 4.942651125088615E-4 82-83 0.0024713255625443075 0.0 0.0 2.780594304371281 4.942651125088615E-4 84-85 0.0025066302134377975 0.0 0.0 2.994363965531363 4.942651125088615E-4 86-87 0.0025419348643312876 0.0 0.0 3.213394019674576 4.942651125088615E-4 88-89 0.0026125441661182676 0.0 0.0 3.451488585300273 4.942651125088615E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3840 0.0 51.962395 1 GTATCAA 6295 0.0 36.678505 1 TAAGGTG 980 0.0 31.019371 5 TACGCTC 80 4.4950427E-4 29.686506 4 TATCAAC 7880 0.0 28.99946 2 ATCAACG 7985 0.0 28.612068 3 TCAACGC 8145 0.0 28.10833 4 GTAAGGT 1000 0.0 28.024063 4 CAACGCA 8275 0.0 27.66675 5 GTACCGT 70 0.008393587 27.141949 6 AACGCAG 8550 0.0 26.832438 6 GGACCGA 475 0.0 24.999163 6 AGGTAAG 950 0.0 24.50437 2 ACCGAGT 510 0.0 24.214876 8 ACGCAGA 9585 0.0 23.88548 7 CGCAGAG 9630 0.0 23.773867 8 GGTAAGG 1105 0.0 23.641743 3 AAGGTAA 995 0.0 22.918661 1 GAGTACT 6800 0.0 22.037865 12-13 GCAGAGT 10395 0.0 21.97858 9 >>END_MODULE