FastQCFastQC Report
Thu 26 May 2016
SRR937920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409495
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76980.5461530548175055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63270.44888417482857335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39560.28066789878644477No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21840.15494911298018085No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21160.1501246900485635No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20050.14224952908665872No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17160.12174573162728494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.12025583631016784No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA16240.11521857119039089No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA15630.11089078003114591No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC15350.10890425294165641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.052.0072861
GTATCAA63250.040.9282951
TATCAAC79150.032.2637672
ATCAACG78950.032.16483
TCAACGC80550.031.584934
CAACGCA81200.031.2735335
AACGCAG83850.030.2819296
CGCAGAG94250.026.8328938
ACGCAGA94550.026.7506087
GCAGAGT101250.024.6960779
GAGTACT65200.024.02227812-13
GTACTTT68650.022.7449914-15
TGGTATC17500.022.5544242
TAAGGTG8900.022.4414525
CAGAGTA97100.022.2736210-11
GTGGTAT18450.022.1813181
AGAGTAC89000.021.49683610-11
TACATGG49000.021.3510062
GTACATG51050.021.25321
AGTACTT66450.021.20977612-13
Produced by FastQC (version 0.11.5)