##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937920_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1409495 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53563581282658 33.0 31.0 34.0 31.0 34.0 2 32.68208258986375 34.0 31.0 34.0 31.0 34.0 3 32.711239131745764 34.0 31.0 34.0 31.0 34.0 4 36.190485244715305 37.0 37.0 37.0 35.0 37.0 5 36.195806299419296 37.0 37.0 37.0 35.0 37.0 6 36.24389586341207 37.0 37.0 37.0 35.0 37.0 7 36.203875856246384 37.0 37.0 37.0 35.0 37.0 8 36.20392126257986 37.0 37.0 37.0 35.0 37.0 9 37.947193853117604 39.0 38.0 39.0 37.0 39.0 10-11 37.881672159177576 39.0 38.0 39.0 35.0 39.0 12-13 37.76130351650769 39.0 38.0 39.0 35.0 39.0 14-15 39.084461456053404 40.0 39.0 41.0 36.0 41.0 16-17 38.9259876764373 40.0 38.0 41.0 36.0 41.0 18-19 39.0919935863554 40.0 39.0 41.0 36.0 41.0 20-21 39.09446291047503 40.0 39.0 41.0 36.0 41.0 22-23 39.00031110433169 40.0 39.0 41.0 36.0 41.0 24-25 38.897299032632255 40.0 39.0 41.0 35.5 41.0 26-27 38.741149134973874 40.0 38.0 41.0 35.0 41.0 28-29 38.57537770619974 40.0 38.0 41.0 35.0 41.0 30-31 38.35877778920819 40.0 38.0 41.0 34.5 41.0 32-33 38.07262530196985 40.0 38.0 41.0 34.0 41.0 34-35 37.84729459842 40.0 38.0 41.0 33.5 41.0 36-37 37.60502875143225 40.0 37.0 41.0 33.0 41.0 38-39 37.32015225311193 40.0 37.0 41.0 33.0 41.0 40-41 37.22050308798541 40.0 37.0 41.0 32.5 41.0 42-43 37.00817278528835 40.0 36.5 41.0 31.5 41.0 44-45 36.68636320100462 39.0 36.0 41.0 31.0 41.0 46-47 36.298851361657896 39.0 35.5 41.0 30.0 41.0 48-49 35.877280870098865 39.0 35.0 40.5 29.0 41.0 50-51 35.139300600569705 38.5 34.5 39.5 27.5 40.5 52-53 35.19569420253353 38.0 35.0 40.0 28.0 40.5 54-55 35.27490874391182 38.5 35.0 40.0 27.5 41.0 56-57 34.96501371058429 38.0 35.0 40.0 26.5 41.0 58-59 34.579965164828536 38.0 34.0 40.0 26.0 41.0 60-61 34.10581378436958 37.5 34.0 40.0 24.5 41.0 62-63 33.58439831287092 37.0 33.0 39.5 22.5 41.0 64-65 33.07145999098968 36.0 33.0 39.0 21.5 41.0 66-67 32.54331303055349 35.5 32.0 39.0 19.0 40.0 68-69 31.90658852993448 35.0 31.5 38.5 14.0 40.0 70-71 31.33743787668633 35.0 31.0 37.5 9.0 40.0 72-73 30.68613013880858 35.0 30.0 37.0 6.0 39.0 74-75 30.052325123537152 34.0 29.5 36.0 2.0 39.0 76-77 29.356227585057063 34.0 29.0 36.0 2.0 38.5 78-79 28.648285378805884 34.0 27.5 35.0 2.0 37.0 80-81 27.97317762744813 33.5 26.5 35.0 2.0 37.0 82-83 27.2790077297188 33.0 25.5 35.0 2.0 36.0 84-85 26.603344105512967 32.5 24.5 35.0 2.0 36.0 86-87 25.904575752308453 32.0 23.0 35.0 2.0 35.0 88-89 25.0546773844533 31.5 19.0 34.0 2.0 35.0 90-91 24.19128765976467 31.0 13.5 34.0 2.0 35.0 92-93 23.302019872365634 30.5 4.5 34.0 2.0 35.0 94-95 22.239755373378408 29.5 2.0 34.0 2.0 35.0 96-97 20.97949939517345 29.0 2.0 34.0 2.0 35.0 98-99 19.399382402917357 28.0 2.0 34.0 2.0 35.0 100-101 15.12579328057212 12.5 2.0 29.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04462315733652389 1101 2 0.047779860814536335 1101 3 -0.09364849542445342 1101 4 -0.16292784313046837 1101 5 -0.149448723990524 1101 6 -0.05358523477278965 1101 7 -0.002661168168415884 1101 8 0.03623043858064534 1101 9 -0.07343913171306582 1101 10-11 0.06308002467266505 1101 12-13 0.0667743204056066 1101 14-15 0.0836156148476519 1101 16-17 0.11788140349256082 1101 18-19 0.11619609330940506 1101 20-21 0.10401658059104335 1101 22-23 0.12472037125397861 1101 24-25 0.1659378461373393 1101 26-27 0.12442128888911697 1101 28-29 0.0398602560604715 1101 30-31 -0.013552893897347928 1101 32-33 0.01755755809519144 1101 34-35 0.002663991758332429 1101 36-37 -0.14041491812577078 1101 38-39 -0.05981925411881406 1101 40-41 0.10950176110512899 1101 42-43 0.08012334254940612 1101 44-45 0.07088395460779395 1101 46-47 0.08872802561713655 1101 48-49 0.09149542703523394 1101 50-51 0.09335304311299097 1101 52-53 -0.004082876843249039 1101 54-55 -0.0013165799422054647 1101 56-57 0.1701487768547949 1101 58-59 0.10229037492274529 1101 60-61 0.06921658928111896 1101 62-63 -0.09970301704056084 1101 64-65 -0.22612926967603642 1101 66-67 -0.08714584245753798 1101 68-69 -0.027389953253656785 1101 70-71 -0.2818083663027622 1101 72-73 -0.4810229606104137 1101 74-75 -0.4588579540836619 1101 76-77 -0.38725241278672584 1101 78-79 -0.6014273514592077 1101 80-81 -0.6606719329523401 1101 82-83 -0.8459537359639455 1101 84-85 -0.6574794365080443 1101 86-87 -0.5552092932692929 1101 88-89 -0.4645648672898197 1101 90-91 -0.5070863123776057 1101 92-93 -0.5581070359639675 1101 94-95 -0.7344246444411269 1101 96-97 -0.8315849625471294 1101 98-99 -1.073623704675473 1101 100-101 -1.6692750128350493 1102 1 0.27309367012076535 1102 2 0.2774858846382031 1102 3 0.1390001195771049 1102 4 0.13413711079723356 1102 5 0.08181347785991022 1102 6 0.08589075764438547 1102 7 0.13382979185195154 1102 8 0.11477970340441601 1102 9 0.23262807631492421 1102 10-11 0.23663160018502083 1102 12-13 0.2110956853152075 1102 14-15 0.22069839408728598 1102 16-17 0.157335232162076 1102 18-19 0.2004089030124092 1102 20-21 0.14929664547894816 1102 22-23 0.18778330686593137 1102 24-25 0.21102220984916187 1102 26-27 0.1347557256941414 1102 28-29 0.11455680134525181 1102 30-31 0.03657254348299688 1102 32-33 0.06548312562860303 1102 34-35 0.07260240483400793 1102 36-37 0.058990433240360574 1102 38-39 -0.10841680761832606 1102 40-41 0.22831831129422397 1102 42-43 0.26904807068981995 1102 44-45 0.32182237711484163 1102 46-47 0.17468328314102877 1102 48-49 0.11742701179034754 1102 50-51 0.07323118965781106 1102 52-53 0.09798118110315812 1102 54-55 0.056566169117012066 1102 56-57 0.20797097644170748 1102 58-59 0.05791808055045067 1102 60-61 0.1378723344074544 1102 62-63 -0.06272021044990339 1102 64-65 -0.0049633622356139995 1102 66-67 -0.013194376349211723 1102 68-69 -0.40737090186205904 1102 70-71 -0.4511818395978011 1102 72-73 -0.4580363248786803 1102 74-75 -0.2858816928721062 1102 76-77 -0.34870635463282795 1102 78-79 -0.5208833493465796 1102 80-81 -0.6694596231125765 1102 82-83 -0.6480856998606193 1102 84-85 -0.8908118267031782 1102 86-87 -0.8390962487248785 1102 88-89 -0.6918069425711089 1102 90-91 -0.6114987844371349 1102 92-93 -0.983584971245822 1102 94-95 -1.1085982195950947 1102 96-97 -1.2138883889682042 1102 98-99 -1.6380164651466664 1102 100-101 -1.8198706046071127 1103 1 0.0943120784790139 1103 2 0.07527554715028373 1103 3 0.09232363685002554 1103 4 0.01460578711973426 1103 5 0.02454619606697861 1103 6 0.057012982807364665 1103 7 0.030142583456381544 1103 8 0.011711576091741449 1103 9 0.034909499116373865 1103 10-11 0.15181698480118655 1103 12-13 0.07297038046466753 1103 14-15 0.1516526003401637 1103 16-17 0.004304320698274466 1103 18-19 0.1203400689028129 1103 20-21 0.1385192601407752 1103 22-23 0.08038059791719121 1103 24-25 0.008086568930380622 1103 26-27 0.056441461680648786 1103 28-29 0.011778186971419302 1103 30-31 0.07325880170825627 1103 32-33 0.04184447022606719 1103 34-35 0.026088171039376107 1103 36-37 -0.13344313849749767 1103 38-39 -0.1487460614050633 1103 40-41 -0.05733918729369947 1103 42-43 0.03843402946439767 1103 44-45 0.05665970734921899 1103 46-47 0.07558391682451315 1103 48-49 0.03580667226417944 1103 50-51 0.018402712130807686 1103 52-53 0.13585591275863607 1103 54-55 0.1092770445615443 1103 56-57 0.11954302662935135 1103 58-59 0.17279869068755715 1103 60-61 0.06931151285998283 1103 62-63 -0.013744102619263288 1103 64-65 -0.08252392925507479 1103 66-67 -0.24312697205832734 1103 68-69 -0.11601846574503938 1103 70-71 -0.31117317956103463 1103 72-73 -0.40469470670923613 1103 74-75 -0.6393627944206699 1103 76-77 -0.6977433565159288 1103 78-79 -0.7190455759698047 1103 80-81 -0.665377794891608 1103 82-83 -0.8243035048556564 1103 84-85 -0.7969219186881809 1103 86-87 -1.0005123878289979 1103 88-89 -0.7258708524256043 1103 90-91 -0.7795483697390004 1103 92-93 -1.1373427072242208 1103 94-95 -1.3320908002556315 1103 96-97 -1.4316971867137553 1103 98-99 -1.5880240198625835 1103 100-101 -1.7220096539703373 1104 1 0.21471562756115503 1104 2 0.12157523560513539 1104 3 0.1666851046211022 1104 4 0.07689838437006102 1104 5 0.17515502856047505 1104 6 0.1393810546009817 1104 7 0.15098304972048027 1104 8 0.2238305180948501 1104 9 0.24935657172292736 1104 10-11 0.24852792638671417 1104 12-13 0.146612674821192 1104 14-15 0.15764498715493147 1104 16-17 0.31030777543723076 1104 18-19 0.2855648444269505 1104 20-21 0.2340700029483216 1104 22-23 0.2554862748439959 1104 24-25 0.3303879045243434 1104 26-27 0.1803521894474187 1104 28-29 0.1974916717629256 1104 30-31 0.07386777919638376 1104 32-33 0.007639509669445488 1104 34-35 0.13971636225057438 1104 36-37 0.11385971954018714 1104 38-39 -0.004219995718628411 1104 40-41 0.027226036611857296 1104 42-43 -0.0017638012224665545 1104 44-45 -0.20787576365619742 1104 46-47 -0.2911193690440186 1104 48-49 -0.2903652952454294 1104 50-51 -0.22415761288917935 1104 52-53 -0.16891702080192061 1104 54-55 -0.21827648020982338 1104 56-57 -0.014670053627114044 1104 58-59 -0.11389486120747705 1104 60-61 -0.11956696326568306 1104 62-63 0.007730811858060349 1104 64-65 -0.09845328206666437 1104 66-67 -0.26922987378772945 1104 68-69 -0.180058973380941 1104 70-71 -0.29738805581777683 1104 72-73 -0.2812537615761741 1104 74-75 -0.4344140790248474 1104 76-77 -0.6227168466625734 1104 78-79 -0.274144989128164 1104 80-81 -0.4983106208081445 1104 82-83 -0.4361361791077982 1104 84-85 -0.5449316255960177 1104 86-87 -0.447841393233432 1104 88-89 -0.6302555870032478 1104 90-91 -0.7381047934365377 1104 92-93 -1.2476879781273702 1104 94-95 -1.398535319023253 1104 96-97 -1.2796352662694126 1104 98-99 -1.7536347227869307 1104 100-101 -1.6086654105958669 1105 1 0.17894662475420375 1105 2 0.08985170310158708 1105 3 0.17529512538430936 1105 4 0.07558996688423036 1105 5 0.08959160758926288 1105 6 0.0049858901541455225 1105 7 0.12873529285501206 1105 8 0.14636735694329417 1105 9 0.11307892627797145 1105 10-11 0.10370829043244356 1105 12-13 0.15540814792035462 1105 14-15 0.10527147683110627 1105 16-17 0.17310706195829795 1105 18-19 0.08022624014682123 1105 20-21 0.19401649202806226 1105 22-23 0.1893934853053736 1105 24-25 0.10350980355626405 1105 26-27 0.14444759209332858 1105 28-29 0.1603369020034009 1105 30-31 0.16613031633345088 1105 32-33 0.08949062219707571 1105 34-35 0.027922152836680425 1105 36-37 -0.00516153944819564 1105 38-39 0.07810872646818012 1105 40-41 0.12574642518575985 1105 42-43 0.23482957915425118 1105 44-45 0.22251484183170334 1105 46-47 0.11140678998648212 1105 48-49 -0.11253374560943286 1105 50-51 0.01321904732859025 1105 52-53 0.05795548863844857 1105 54-55 0.2815292142020027 1105 56-57 0.0381279468425717 1105 58-59 0.04842858630485125 1105 60-61 0.04396285635163366 1105 62-63 0.07498524127993988 1105 64-65 0.08172670921055669 1105 66-67 -0.04042222979656884 1105 68-69 -0.36559425032930903 1105 70-71 -0.4452690531972152 1105 72-73 -0.5047666947797822 1105 74-75 -0.632505597422842 1105 76-77 -0.5270402158935177 1105 78-79 -0.5519870694746913 1105 80-81 -0.4184477623446199 1105 82-83 -0.5992084253735896 1105 84-85 -1.0161417813014246 1105 86-87 -0.6464291597163268 1105 88-89 -0.4235978481425029 1105 90-91 -0.47075335100877425 1105 92-93 -0.6626711289905209 1105 94-95 -0.6969248658485689 1105 96-97 -0.8665429836959682 1105 98-99 -0.595461120762053 1105 100-101 -0.4666872026432234 1106 1 0.18366631152162682 1106 2 0.11200991548125216 1106 3 0.18549929184315062 1106 4 0.14395372567519615 1106 5 0.1487238170095182 1106 6 0.06349878356529359 1106 7 0.14595144661164738 1106 8 -0.025669486124527907 1106 9 0.17517470827873893 1106 10-11 0.09804923844315283 1106 12-13 0.10634086276897392 1106 14-15 0.2513251037784201 1106 16-17 -0.1397327305500582 1106 18-19 0.23770396139642713 1106 20-21 0.31232039745975726 1106 22-23 0.280686484124935 1106 24-25 0.2922120663457619 1106 26-27 0.22661814922834367 1106 28-29 0.14252325377488972 1106 30-31 0.15826105435862559 1106 32-33 0.21860450152605893 1106 34-35 0.14596419982157016 1106 36-37 0.08769221884399059 1106 38-39 0.16572635494507892 1106 40-41 0.08755042206008312 1106 42-43 -0.013224147548655196 1106 44-45 0.27628207045490427 1106 46-47 0.39340755477940803 1106 48-49 0.40661932935689293 1106 50-51 0.24390594775588426 1106 52-53 0.3720557347022506 1106 54-55 0.2540122557502116 1106 56-57 0.2044641730882759 1106 58-59 0.3915286162315397 1106 60-61 0.45218770547128884 1106 62-63 0.21847639628351345 1106 64-65 0.04767575903847643 1106 66-67 -0.017766278543810188 1106 68-69 -0.049568513384823376 1106 70-71 -0.038678741128450156 1106 72-73 -0.09850266664870233 1106 74-75 0.14399717910799126 1106 76-77 -0.17056981836745777 1106 78-79 -0.3871108079820118 1106 80-81 -0.35834397495705517 1106 82-83 -0.25856383794612015 1106 84-85 -0.4162043363748289 1106 86-87 -0.7499422193867993 1106 88-89 -0.5545104456120349 1106 90-91 -0.6782055098861903 1106 92-93 -0.5946180673249337 1106 94-95 -1.0052891649823614 1106 96-97 -0.9576759579091458 1106 98-99 -1.4058808008790997 1106 100-101 -1.6806110059916861 1107 1 -0.018973062500791116 1107 2 -0.04791665939049494 1107 3 0.020969463897834828 1107 4 -0.01833019292502769 1107 5 0.01755035217010459 1107 6 -0.11743519865382979 1107 7 -0.019674490450213966 1107 8 -0.049261158126533644 1107 9 -0.09496756303764897 1107 10-11 -0.060593814939998936 1107 12-13 -0.15463846311379115 1107 14-15 -0.0718553753489104 1107 16-17 -0.06760429330585538 1107 18-19 -0.03645349336121484 1107 20-21 -0.040973197697105945 1107 22-23 0.13253751053691332 1107 24-25 -0.05355362969365274 1107 26-27 -0.05744987437142868 1107 28-29 -0.06798288119443185 1107 30-31 -0.11399188548302419 1107 32-33 -0.17778938052369142 1107 34-35 0.030628599980410343 1107 36-37 0.03377317288439485 1107 38-39 0.010281974884946976 1107 40-41 0.03425034344818556 1107 42-43 0.14095595878000466 1107 44-45 0.2901845228217681 1107 46-47 0.18335272906278277 1107 48-49 0.30795228719012613 1107 50-51 0.09681863992953765 1107 52-53 0.09569897043257214 1107 54-55 0.04847001604067458 1107 56-57 -0.09729912407278363 1107 58-59 -0.08914511605039621 1107 60-61 -0.14739208559937111 1107 62-63 -0.17570703281585764 1107 64-65 -0.4144512396891429 1107 66-67 -0.2935121797845781 1107 68-69 -0.35070879103878383 1107 70-71 -0.4488033652906651 1107 72-73 -0.5480456843728483 1107 74-75 -0.685404377731647 1107 76-77 -0.7743283988149763 1107 78-79 -1.0116463292112776 1107 80-81 -0.8342462895504994 1107 82-83 -0.60959835423985 1107 84-85 -0.6699277643395938 1107 86-87 -0.7346473437475716 1107 88-89 -0.49954796860803796 1107 90-91 -0.6299432784430543 1107 92-93 -0.7292211785729172 1107 94-95 -0.7175691327271707 1107 96-97 -0.8215335326107045 1107 98-99 -1.003428940532718 1107 100-101 -1.4405499399495927 1108 1 -0.0513802154269456 1108 2 -0.09531499084723549 1108 3 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-0.2205929463003713 2316 68-69 -0.2427963849032473 2316 70-71 -0.24840724937580916 2316 72-73 -0.15383623947242242 2316 74-75 -0.12092669306437287 2316 76-77 -0.05182955214592866 2316 78-79 0.1552397328073596 2316 80-81 0.17236475167112175 2316 82-83 0.06011684249339666 2316 84-85 0.06711648466131948 2316 86-87 0.006392803285912407 2316 88-89 -0.010841811395597034 2316 90-91 -0.0755426492523874 2316 92-93 -0.10076002776169446 2316 94-95 0.3245885873645058 2316 96-97 0.10863204680608973 2316 98-99 0.2081146516716892 2316 100-101 0.3038784062386721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3224.0 3 683.0 4 564.0 5 623.0 6 722.0 7 888.0 8 1092.0 9 1413.0 10 1936.0 11 2645.0 12 3808.0 13 5141.0 14 6423.0 15 8543.0 16 11073.0 17 15037.0 18 14731.0 19 11478.0 20 11215.0 21 11631.0 22 12698.0 23 13978.0 24 15955.0 25 18444.0 26 21284.0 27 25106.0 28 30189.0 29 35899.0 30 43519.0 31 54067.0 32 68608.0 33 90096.0 34 121132.0 35 168322.0 36 216745.0 37 233137.0 38 119859.0 39 7587.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89523133654564 18.010088718494067 14.467031940504835 23.62764800445546 2 15.727785676613996 19.190125973621278 40.35968531793664 24.72240303182809 3 19.43965446803552 23.058816173612904 29.415191839443306 28.086337518908266 4 12.924311380818466 15.998298030730632 35.85316181991375 35.22422876853715 5 13.932184957868813 36.705771137559836 33.81739479641627 15.544649108155076 6 33.10793953390183 35.18986460023698 16.69755080600851 15.004645059852681 7 29.204080008413563 32.49481969114274 19.728023125922007 18.573077174521693 8 26.888665804304335 34.29352788076113 19.017716292258477 19.80009002267606 9 26.35755662183328 16.611178394735067 19.778591495271005 37.252673488160646 10-11 25.513696198072903 25.94884840014544 26.378035235433146 22.159420166348514 12-13 26.43941450050516 23.33219891977304 27.05690019481599 23.17148638490581 14-15 22.845600479679 24.89166017811777 25.700905365698407 26.561833976504822 16-17 22.417161958596164 27.996766290999002 26.559143948002074 23.026927802402763 18-19 22.311095451742784 26.010514665977148 29.30488979561683 22.37350008666324 20-21 22.6642915877095 25.184657652969705 29.85879172890001 22.292259030420784 22-23 22.398548888477432 25.085763491424363 29.815487218014653 22.700200402083556 24-25 22.27934814831666 25.37353222824051 29.88520864272923 22.4619109807136 26-27 21.697483827651254 25.773732766368617 29.720105577639703 22.80867782834042 28-29 21.857118248864786 26.293204335217517 29.54589608761359 22.30378132830411 30-31 22.00273338231028 25.653101898915985 29.46187023997165 22.882294478802084 32-33 21.644260441864272 25.729198223791855 29.54187311611732 23.084668218226557 34-35 22.65043814176762 25.87958557611737 29.136179006121203 22.33379727599381 36-37 22.670213260744607 25.440611176812887 29.198050556717515 22.69112500572499 38-39 22.071555375419415 25.80956565500979 29.079344212956883 23.03953475661391 40-41 22.57057430533077 25.72231828997507 29.230731553571733 22.476375851122434 42-43 22.28801758834013 25.765079662558865 28.92109205873239 23.025810690368615 44-45 22.22894105923885 26.107756424178163 29.07495099270817 22.58835152387482 46-47 22.72278468163882 26.42066648542405 28.273884097947676 22.582664734989454 48-49 22.53583906622353 26.468332620603626 28.170224696173086 22.825603616999757 50-51 22.64477669574871 26.72460360895616 28.08809510119799 22.54252459409713 52-53 22.981286458407254 26.358559121166103 28.078437912485633 22.581716507941014 54-55 22.370319789953513 26.635984017955312 28.21821779901209 22.775478393079084 56-57 22.55485055852938 26.746925622380065 27.97547026676422 22.722753552326335 58-59 22.60769697879546 26.287109368761424 27.948777190645686 23.156416461797434 60-61 22.19082104689405 26.588069887566128 28.36119931355864 22.859909751981185 62-63 22.20799810334566 26.628792731952416 28.46976124656755 22.69344791813438 64-65 22.014040967430677 26.681564301321163 28.26806657588975 23.03632815535841 66-67 21.953192895222163 26.712150606584622 28.16325531202903 23.171401186164186 68-69 22.48145417577881 26.276732555708467 28.371641314761302 22.870171953751424 70-71 22.332769260691 26.504221317119086 27.910096663690144 23.252912758499775 72-73 22.255039330843303 26.679528512554494 27.922023994582695 23.143408162019508 74-75 22.189904427100245 27.101026350240907 27.785129721287404 22.923939501371436 76-77 22.41614829259431 26.681398283014502 27.776476816154254 23.125976608236932 78-79 22.512008765387506 26.921278276216153 27.705422802922836 22.861290155473508 80-81 22.736632121796834 27.014438305526056 27.358763381893908 22.8901661907832 82-83 22.605737423683806 27.077643732749358 27.44136421971116 22.875254623855678 84-85 22.610263267977288 27.157428935457283 27.366727704749298 22.86558009181613 86-87 22.785780256127406 27.128194629293674 27.093777487144134 22.992247627434793 88-89 22.60835365764919 27.22760240278316 26.955597921790638 23.208446017777018 90-91 22.6614029842054 27.157916865824394 26.96257065808341 23.218109491886793 92-93 22.823974637977003 26.697256825984 27.223951917239713 23.25481661879928 94-95 22.62495740088606 26.562925991139387 27.39243723730546 23.41967937066909 96-97 22.860572861424785 26.302874246225628 27.340232489787574 23.496320402562016 98-99 22.64514981804475 26.54878469936123 26.898604447779217 23.9074610348148 100-101 23.565930625363393 25.42537348155741 26.998043308623398 24.010652584455794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 657.0 1 477.5 2 440.5 3 606.0 4 492.5 5 433.5 6 827.0 7 1762.5 8 2877.5 9 3283.0 10 2572.5 11 1778.0 12 1629.0 13 1742.0 14 1883.5 15 1942.0 16 2024.0 17 2178.5 18 2338.5 19 2513.0 20 2815.0 21 3331.5 22 3993.0 23 4752.5 24 5715.0 25 7092.5 26 8946.5 27 11156.0 28 12848.0 29 14384.0 30 16147.5 31 18738.5 32 22782.0 33 24221.0 34 24037.5 35 25563.5 36 27716.0 37 30031.0 38 32061.0 39 33375.5 40 35064.0 41 38074.5 42 39991.5 43 41491.0 44 43441.0 45 45895.0 46 47454.5 47 49069.0 48 50743.5 49 50089.5 50 49159.0 51 48454.0 52 48517.5 53 50132.5 54 51964.5 55 49850.0 56 45523.5 57 41834.0 58 39328.0 59 35481.5 60 29070.0 61 24685.0 62 21515.5 63 16770.5 64 12331.0 65 9667.5 66 7494.5 67 5931.0 68 4668.0 69 3521.0 70 2765.5 71 2007.5 72 1465.0 73 1156.5 74 917.0 75 734.0 76 522.0 77 313.5 78 190.0 79 135.5 80 81.0 81 45.5 82 30.0 83 19.0 84 14.5 85 10.5 86 6.5 87 5.5 88 4.5 89 3.0 90 3.5 91 5.0 92 4.0 93 1.5 94 1.0 95 1.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1265701545589023 2 0.05023075640566302 3 0.14487458274062695 4 0.12160383683517854 5 0.1830442818172466 6 0.18616596724358725 7 0.15913500934731942 8 0.0681804476071217 9 0.11990109932990185 10-11 0.09698509040471942 12-13 0.14281710825508426 14-15 0.1338777363523815 16-17 0.12937257670300356 18-19 0.12394510090493405 20-21 0.12930162930695038 22-23 0.18258312374290084 24-25 0.2026257631279288 26-27 0.14189479210639272 28-29 0.12220688970163074 30-31 0.09574351097378848 32-33 0.11809194073054534 34-35 0.09045792996782535 36-37 0.08481761198159625 38-39 0.048528018900386306 40-41 0.0544166527728016 42-43 0.05920560200639236 44-45 0.0369635933437153 46-47 0.04980507202934384 48-49 0.04203633216151884 50-51 0.05122401995040777 52-53 0.025186325598884705 54-55 0.04710907097932238 56-57 0.024689693826512333 58-59 0.04749928165761496 60-61 0.03469327667001302 62-63 0.04955675614315766 64-65 0.03877275194307181 66-67 0.035438224328571584 68-69 0.06310770878931816 70-71 0.04916654546486507 72-73 0.048031387128013935 74-75 0.04047548944834852 76-77 0.019936218290948177 78-79 0.02241937715281005 80-81 0.025647483673230484 82-83 0.021887271682411075 84-85 0.018836533652123632 86-87 0.02224200866267706 88-89 0.062327287432733 90-91 0.105144040950837 92-93 0.07733266169798403 94-95 0.07201160699399431 96-97 0.06505876218078106 98-99 0.0545940212629346 100-101 0.12497383814770538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1409495.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.94317489772851 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57645821355537 48.899519022323915 2 11.00268088771358 13.190712495922199 3 3.0946821591954388 5.56515221764597 4 1.354788460424175 3.2484128653052267 5 0.7485255928258978 2.2434500263094357 6 0.45089372407084344 1.6216800817360024 7 0.31773481460822584 1.3332223494210758 8 0.22703193738878133 1.0887212104212693 9 0.1648082408623858 0.8891216285940864 >10 0.9540375424461582 10.510602438942437 >50 0.06685270842934257 2.7527020653592884 >100 0.035102044751950494 4.024976920473188 >500 0.004387702369023303 1.8314221756391276 >1k 0.0017787982577121494 1.8033437810040762 >5k 2.371731010282866E-4 0.9969607209027622 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7698 0.5461530548175055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6327 0.44888417482857335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3956 0.28066789878644477 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2184 0.15494911298018085 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2116 0.1501246900485635 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2005 0.14224952908665872 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1716 0.12174573162728494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1695 0.12025583631016784 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1624 0.11521857119039089 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1563 0.11089078003114591 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1535 0.10890425294165641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.094739605319635E-5 0.0 0.0 0.0 0.0 7 7.094739605319635E-5 0.0 0.0 0.0 0.0 8 7.094739605319635E-5 0.0 0.0 0.0 0.0 9 7.094739605319635E-5 0.0 0.0 0.0 0.0 10-11 7.094739605319635E-5 0.0 0.0 0.0 0.0 12-13 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 14-15 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 16-17 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 18-19 1.0642109407979454E-4 0.0 0.0 0.0 7.094739605319635E-5 20-21 1.418947921063927E-4 0.0 0.0 3.5473698026598176E-5 7.094739605319635E-5 22-23 2.1284218815958907E-4 0.0 0.0 7.094739605319635E-5 1.418947921063927E-4 24-25 3.547369802659818E-4 0.0 0.0 7.094739605319635E-5 1.418947921063927E-4 26-27 3.547369802659818E-4 0.0 0.0 2.4831588618618724E-4 1.418947921063927E-4 28-29 4.2568437631917814E-4 0.0 0.0 0.0016672638072501144 1.418947921063927E-4 30-31 4.966317723723745E-4 0.0 0.0 0.006278844550707877 1.418947921063927E-4 32-33 4.966317723723745E-4 0.0 0.0 0.017027375052767125 2.1284218815958907E-4 34-35 5.321054703989727E-4 0.0 0.0 0.03377096052132147 2.1284218815958907E-4 36-37 5.675791684255708E-4 0.0 0.0 0.06243370852681279 2.1284218815958907E-4 38-39 7.094739605319636E-4 0.0 0.0 0.10581804121334237 2.1284218815958907E-4 40-41 7.094739605319636E-4 0.0 0.0 0.1520757434400264 2.1284218815958907E-4 42-43 7.8042135658516E-4 0.0 0.0 0.19964597249369453 2.8378958421278546E-4 44-45 8.513687526383563E-4 0.0 0.0 0.25792925835139535 3.547369802659818E-4 46-47 9.223161486915526E-4 0.0 0.0 0.32582591637430425 3.9021067829258E-4 48-49 9.223161486915526E-4 0.0 0.0 0.3997531030617349 4.2568437631917814E-4 50-51 9.223161486915526E-4 0.0 0.0 0.48393218847885233 4.2568437631917814E-4 52-53 9.223161486915526E-4 0.0 0.0 0.560058744443932 4.2568437631917814E-4 54-55 9.223161486915526E-4 0.0 0.0 0.6427479345439324 4.2568437631917814E-4 56-57 9.223161486915526E-4 0.0 0.0 0.7459409221033064 4.2568437631917814E-4 58-59 9.223161486915526E-4 0.0 0.0 0.8583215974515696 4.2568437631917814E-4 60-61 9.223161486915526E-4 0.0 0.0 0.9762716433900085 4.2568437631917814E-4 62-63 9.93263544744749E-4 0.0 0.0 1.0875171604014202 4.2568437631917814E-4 64-65 0.0010642109407979453 0.0 0.0 1.2069216279589499 4.2568437631917814E-4 66-67 0.0010642109407979453 0.0 0.0 1.345410945054789 4.2568437631917814E-4 68-69 0.0010642109407979453 0.0 0.0 1.4968481619303367 4.2568437631917814E-4 70-71 0.0012061057329043382 0.0 0.0 1.6591403304020234 4.2568437631917814E-4 72-73 0.0013125268269841327 0.0 0.0 1.8247670264882103 5.321054703989727E-4 74-75 0.001383474223037329 0.0 0.0 1.9926640392480994 5.675791684255708E-4 76-77 0.0014189479210639272 0.0 0.0 2.172551161940979 5.675791684255708E-4 78-79 0.0014189479210639272 0.0 0.0 2.369572080780705 5.675791684255708E-4 80-81 0.00156084271317032 0.0 0.0 2.586848481193619 5.675791684255708E-4 82-83 0.00156084271317032 0.0 0.0 2.813277095697395 6.385265644787673E-4 84-85 0.00156084271317032 0.0 0.0 3.0431111852117247 6.385265644787673E-4 86-87 0.00156084271317032 0.0 0.0 3.279756224747161 6.385265644787673E-4 88-89 0.00156084271317032 0.0 0.0 3.527007899992551 6.385265644787673E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3660 0.0 52.007286 1 GTATCAA 6325 0.0 40.928295 1 TATCAAC 7915 0.0 32.263767 2 ATCAACG 7895 0.0 32.1648 3 TCAACGC 8055 0.0 31.58493 4 CAACGCA 8120 0.0 31.273533 5 AACGCAG 8385 0.0 30.281929 6 CGCAGAG 9425 0.0 26.832893 8 ACGCAGA 9455 0.0 26.750608 7 GCAGAGT 10125 0.0 24.696077 9 GAGTACT 6520 0.0 24.022278 12-13 GTACTTT 6865 0.0 22.74499 14-15 TGGTATC 1750 0.0 22.554424 2 TAAGGTG 890 0.0 22.441452 5 CAGAGTA 9710 0.0 22.27362 10-11 GTGGTAT 1845 0.0 22.181318 1 AGAGTAC 8900 0.0 21.496836 10-11 TACATGG 4900 0.0 21.351006 2 GTACATG 5105 0.0 21.2532 1 AGTACTT 6645 0.0 21.209776 12-13 >>END_MODULE