FastQCFastQC Report
Thu 26 May 2016
SRR937920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1409495
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77490.5497713720162185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68270.4843578728551715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.3120975952380108No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18570.13174931447078564No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18530.13146552488657284No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18170.1289114186286578No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17610.12493836444967879No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17020.12075246808254021No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16020.11365772847722055No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG14670.10407983001003906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41900.047.8387031
GTATCAA70400.038.7275661
TATCAAC86150.031.3716952
ATCAACG87550.030.8124983
TCAACGC89550.030.1221984
CAACGCA90750.029.7238885
AACGCAG93650.028.8541566
ACGCAGA105100.025.5290267
CGCAGAG105250.025.4475238
GTACCGT1351.3451592E-524.6245446
GAGTACT73350.024.02111412-13
GCAGAGT111750.023.5848949
TACATGG48250.023.134042
GTATAGG2503.8380676E-1022.7993351
GTACATG50250.022.7804321
GTACTTT77000.022.69741614-15
ACATGGG49500.021.7798773
AGAGTAC96300.021.15644310-11
CAGAGTA107350.021.1464510-11
GTATAGC5400.021.1104971
Produced by FastQC (version 0.11.5)