##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937920_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1409495 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88810318589282 31.0 31.0 34.0 30.0 34.0 2 32.17323225694309 33.0 31.0 34.0 30.0 34.0 3 32.22218383179791 34.0 31.0 34.0 30.0 34.0 4 35.86543904022363 37.0 35.0 37.0 35.0 37.0 5 35.806864160568146 37.0 35.0 37.0 35.0 37.0 6 35.85155037797225 37.0 35.0 37.0 35.0 37.0 7 35.79522878761542 37.0 35.0 37.0 35.0 37.0 8 35.773030056864336 37.0 35.0 37.0 33.0 37.0 9 37.32919307979099 39.0 37.0 39.0 34.0 39.0 10-11 37.2611917743589 39.0 37.0 39.0 34.0 39.0 12-13 37.13986285868343 39.0 37.0 39.0 33.0 39.0 14-15 38.13478550828488 40.0 37.5 41.0 33.0 41.0 16-17 37.92101142607814 40.0 37.0 41.0 33.0 41.0 18-19 38.244528359447884 40.0 38.0 41.0 33.5 41.0 20-21 38.204672595504064 40.0 38.0 41.0 33.0 41.0 22-23 38.044542194190115 40.0 37.0 41.0 33.0 41.0 24-25 37.857258450721716 40.0 37.0 41.0 33.0 41.0 26-27 37.56053302778655 39.0 37.0 41.0 32.0 41.0 28-29 37.36993604092246 39.0 36.5 40.5 32.0 41.0 30-31 36.98375304630382 39.0 36.0 40.0 31.0 41.0 32-33 36.643193484191144 38.0 35.0 40.0 30.0 41.0 34-35 36.36755504631091 38.0 35.0 40.0 30.0 41.0 36-37 36.275854827438195 38.0 35.0 40.0 30.0 41.0 38-39 36.00304293381672 38.0 35.0 40.0 30.0 41.0 40-41 35.69618338482932 38.0 35.0 40.0 29.0 41.0 42-43 35.311150802237684 38.0 34.0 40.0 27.5 41.0 44-45 34.85484162767516 38.0 33.5 40.0 26.0 41.0 46-47 34.23326404137652 38.0 33.0 40.0 24.0 41.0 48-49 33.583214200830795 37.0 32.5 40.0 22.5 41.0 50-51 32.941421218237736 37.0 31.5 39.0 19.5 40.0 52-53 32.09834195935424 36.0 30.5 39.0 16.0 40.0 54-55 31.50872120865984 35.0 30.0 39.0 11.0 40.0 56-57 30.92076807650967 35.0 29.0 38.0 8.0 40.0 58-59 30.22134133146978 34.5 28.0 38.0 6.5 40.0 60-61 29.41902064214488 34.0 26.5 38.0 2.0 40.0 62-63 28.661463857622763 33.5 26.0 37.0 2.0 39.0 64-65 27.89430327883391 33.0 24.5 36.5 2.0 39.0 66-67 27.033976353232894 32.0 22.5 36.0 2.0 39.0 68-69 26.138378993894975 31.0 21.0 35.0 2.0 38.0 70-71 25.175265964050954 30.5 18.5 35.0 2.0 38.0 72-73 24.296822266130775 30.0 13.5 34.0 2.0 37.0 74-75 23.45855998070231 29.0 8.0 34.0 2.0 36.0 76-77 22.509755621694296 28.5 7.5 32.5 2.0 35.0 78-79 22.998449799396237 29.0 4.5 34.0 2.0 35.5 80-81 22.43061025402715 29.0 2.0 34.0 2.0 35.0 82-83 21.542687274520308 28.5 2.0 34.0 2.0 35.0 84-85 20.579402551977836 26.5 2.0 33.0 2.0 35.0 86-87 19.548553205225986 25.5 2.0 32.5 2.0 35.0 88-89 18.38973426652808 24.5 2.0 32.0 2.0 34.0 90-91 17.450065448972857 21.5 2.0 31.5 2.0 34.0 92-93 16.253998063136088 14.5 2.0 31.0 2.0 34.0 94-95 15.027000450515965 3.5 2.0 31.0 2.0 34.0 96-97 13.652137112937613 2.0 2.0 30.0 2.0 34.0 98-99 12.104901755593316 2.0 2.0 29.0 2.0 34.0 100-101 9.483633854678448 2.0 2.0 22.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13723931261442246 1101 2 0.07746471528751186 1101 3 0.04102207202600283 1101 4 0.11786034984879024 1101 5 0.18229263536359497 1101 6 0.06127125086256058 1101 7 0.005116126285855671 1101 8 0.16036184241851714 1101 9 0.03394794473607732 1101 10-11 0.029613289557659073 1101 12-13 0.2564923138768975 1101 14-15 0.23005684283756977 1101 16-17 0.20638317031244924 1101 18-19 0.18238809988752536 1101 20-21 0.33272065442075416 1101 22-23 0.3252188969929435 1101 24-25 0.2767749378991553 1101 26-27 0.36157125733497963 1101 28-29 0.2570720425716573 1101 30-31 0.37487051024511686 1101 32-33 0.3398028010994736 1101 34-35 0.37725162352835895 1101 36-37 0.5535399181633878 1101 38-39 0.5672276927060338 1101 40-41 0.42995482711558 1101 42-43 0.4120240462364606 1101 44-45 0.20593062410993923 1101 46-47 0.37688335354283 1101 48-49 0.50518201649475 1101 50-51 0.37455172648110846 1101 52-53 0.22775507079953883 1101 54-55 0.07816418027709915 1101 56-57 0.25105695643430437 1101 58-59 0.21945650262345495 1101 60-61 0.27305516241761296 1101 62-63 0.12536632403628545 1101 64-65 0.11896858256484677 1101 66-67 -0.15751565792977473 1101 68-69 -0.1294906150275139 1101 70-71 -0.4058919050449923 1101 72-73 -0.7788462843141097 1101 74-75 -0.47612852031758734 1101 76-77 -0.5082330436565776 1101 78-79 -0.5138653696162372 1101 80-81 -0.8633890767298773 1101 82-83 -0.9756712251880089 1101 84-85 -0.8557559397443448 1101 86-87 -1.0366811874830724 1101 88-89 -1.039637880077965 1101 90-91 -1.0630256087551118 1101 92-93 -1.1788082276770862 1101 94-95 -0.9604034318628969 1101 96-97 -1.1080633438660445 1101 98-99 -0.9773667462797668 1101 100-101 -0.9037691236531504 1102 1 0.2726833892349738 1102 2 0.22014266188702436 1102 3 0.21751623871624304 1102 4 0.13093696096461116 1102 5 0.19320002862392727 1102 6 0.17946532984095853 1102 7 0.1239798333848583 1102 8 0.14348438436458366 1102 9 0.2625740753229948 1102 10-11 0.33257049937761707 1102 12-13 0.25066843550400364 1102 14-15 0.309190853344127 1102 16-17 0.28818390407396777 1102 18-19 0.30903033927486234 1102 20-21 0.2613722497390185 1102 22-23 0.3624987921159786 1102 24-25 0.25170160890426274 1102 26-27 0.033776503069638864 1102 28-29 0.07151667564374264 1102 30-31 0.26468367456808295 1102 32-33 0.13348896243269337 1102 34-35 0.3301984588281357 1102 36-37 0.29316775583043153 1102 38-39 0.20640423874532132 1102 40-41 0.13329778045852692 1102 42-43 0.011513336902218896 1102 44-45 0.23620300242153291 1102 46-47 0.1993234406888007 1102 48-49 0.20841415109943284 1102 50-51 0.305471569165654 1102 52-53 0.007881262690432322 1102 54-55 0.12676880731309836 1102 56-57 0.23342970351293602 1102 58-59 0.3344226911190553 1102 60-61 -0.07716034466063704 1102 62-63 -0.038541663401115756 1102 64-65 -0.06695460826030697 1102 66-67 -0.4412965103380806 1102 68-69 -0.25167888427460383 1102 70-71 -0.20741082911293418 1102 72-73 -0.07046461514812918 1102 74-75 -0.5109538982802206 1102 76-77 -0.33446926204965877 1102 78-79 -0.4832982606177545 1102 80-81 -0.8428663895405251 1102 82-83 -0.737957958062978 1102 84-85 -1.0200223196970022 1102 86-87 -1.2078466233543885 1102 88-89 -1.0957569607068542 1102 90-91 -1.4487974258994782 1102 92-93 -1.794932118310781 1102 94-95 -1.8014044787462975 1102 96-97 -2.016286565814756 1102 98-99 -1.6513356604447598 1102 100-101 -1.6760649106548193 1103 1 0.13836155428066732 1103 2 0.12631106208213083 1103 3 0.09572357054269531 1103 4 0.03889669077958047 1103 5 0.016868395862218222 1103 6 -0.006771093219079205 1103 7 0.03949377208923721 1103 8 0.08594634966562609 1103 9 0.0445115101065241 1103 10-11 0.23917367600889605 1103 12-13 0.26720464388841947 1103 14-15 0.13239681112492718 1103 16-17 0.18407408791214408 1103 18-19 0.30655477548517496 1103 20-21 0.21010108005604167 1103 22-23 0.22506405928420037 1103 24-25 0.24241285752133734 1103 26-27 0.36435236509314706 1103 28-29 0.16745168074951522 1103 30-31 0.33730731078975396 1103 32-33 0.31573431526835094 1103 34-35 0.18552666306709398 1103 36-37 0.1219175568219768 1103 38-39 0.11777347776740754 1103 40-41 0.08634065778109346 1103 42-43 -0.002433733439794139 1103 44-45 -0.36594055949012017 1103 46-47 -0.5111083544232429 1103 48-49 -0.48195252134735256 1103 50-51 -0.5720614155352877 1103 52-53 -0.8137280641894478 1103 54-55 -0.6971277224864565 1103 56-57 -0.7646253374092886 1103 58-59 -0.5167983335032353 1103 60-61 -0.7808091203403329 1103 62-63 -1.074650670346383 1103 64-65 -1.226718850420891 1103 66-67 -1.2059448002850353 1103 68-69 -1.1960741470114016 1103 70-71 -1.107081920105177 1103 72-73 -1.3139378983262588 1103 74-75 -1.3066373668378972 1103 76-77 -1.3018237627843412 1103 78-79 -1.3010257817887059 1103 80-81 -1.3671023238256517 1103 82-83 -1.4083137598312874 1103 84-85 -1.7338669196976078 1103 86-87 -1.6355195984623627 1103 88-89 -1.7397181568465463 1103 90-91 -1.8284873073462116 1103 92-93 -1.900022779972069 1103 94-95 -1.6126922383826177 1103 96-97 -1.4148236888775294 1103 98-99 -1.4996640908181664 1103 100-101 -1.3869806639107 1104 1 0.17757728793904803 1104 2 0.2492418677400181 1104 3 0.23197503378026596 1104 4 0.09451953458844997 1104 5 0.15134802459861163 1104 6 0.1520483024239283 1104 7 0.13230339122698354 1104 8 0.1742548518613276 1104 9 0.2404616164842608 1104 10-11 0.21822702533675198 1104 12-13 0.3094087170778508 1104 14-15 0.315403004329319 1104 16-17 0.3713639060985372 1104 18-19 0.3288815334726891 1104 20-21 0.44356854954192215 1104 22-23 0.28808646288934625 1104 24-25 0.22986405474208738 1104 26-27 0.28955446345557334 1104 28-29 0.20955256608678496 1104 30-31 0.2568925584550925 1104 32-33 0.1530210885673 1104 34-35 0.13759795983148848 1104 36-37 0.033867145085530126 1104 38-39 0.1489714911475133 1104 40-41 0.015593423070541235 1104 42-43 0.1018220042397715 1104 44-45 0.16284860960359282 1104 46-47 0.009005543699657892 1104 48-49 0.11108449771751339 1104 50-51 0.34773890751277037 1104 52-53 -0.09960795449617166 1104 54-55 -0.01635033855708201 1104 56-57 0.2041201013009939 1104 58-59 -0.05309266682271385 1104 60-61 -0.11619283898886579 1104 62-63 0.048254277934031364 1104 64-65 -0.24234081459561807 1104 66-67 -0.3058161417077798 1104 68-69 -0.17161016802982232 1104 70-71 -0.3553953907838796 1104 72-73 -0.7757965749505153 1104 74-75 -0.7154785116769098 1104 76-77 -0.6386458282619891 1104 78-79 -0.5674200840793304 1104 80-81 -0.6721753962910739 1104 82-83 -0.7763944947801207 1104 84-85 -1.0942808213970459 1104 86-87 -1.0681359977428002 1104 88-89 -1.3648052744594317 1104 90-91 -1.365091929195355 1104 92-93 -1.1584027451926886 1104 94-95 -1.1776442789888488 1104 96-97 -1.4017664382272308 1104 98-99 -1.588501581792249 1104 100-101 -1.3966967547392084 1105 1 0.143323589143467 1105 2 0.1528495214231569 1105 3 0.13304786156493975 1105 4 0.1126882719842186 1105 5 0.07747835192664354 1105 6 0.1479832617735184 1105 7 0.08248474310927634 1105 8 0.0659758495389795 1105 9 0.16378126921311065 1105 10-11 0.17282847987949168 1105 12-13 0.13976409594112482 1105 14-15 0.07794107331385902 1105 16-17 0.07605389316346844 1105 18-19 0.13820624467027898 1105 20-21 0.09438882665803305 1105 22-23 0.18793521206459474 1105 24-25 0.08621398000200031 1105 26-27 0.19147720345326036 1105 28-29 0.2232122023392762 1105 30-31 0.0792007970120352 1105 32-33 0.1867128388273045 1105 34-35 0.05348973394867329 1105 36-37 -0.01972159059549483 1105 38-39 0.032258601185048974 1105 40-41 0.10384823271630239 1105 42-43 -0.0020587955723527784 1105 44-45 -0.09994154408886402 1105 46-47 -0.15941639670957386 1105 48-49 0.01194158572442916 1105 50-51 -0.0029523916973275277 1105 52-53 -0.24019620185044488 1105 54-55 0.03109517200531542 1105 56-57 -0.1549356574134002 1105 58-59 -0.06999166146603031 1105 60-61 -0.272599052294467 1105 62-63 -0.5368566952527303 1105 64-65 -0.752362696107415 1105 66-67 -0.42650134615267277 1105 68-69 -0.5260911251502591 1105 70-71 -0.5657547209934108 1105 72-73 -0.564424246302881 1105 74-75 -1.0360524447202302 1105 76-77 -0.991496855052862 1105 78-79 -1.2211647301915427 1105 80-81 -1.0636676072929632 1105 82-83 -1.0866627762799936 1105 84-85 -1.4139573142417525 1105 86-87 -1.4063209132920953 1105 88-89 -1.8218814459776773 1105 90-91 -1.7886198663316755 1105 92-93 -1.8593128595207915 1105 94-95 -1.5907570638305906 1105 96-97 -1.6516075456479324 1105 98-99 -1.8705442973363233 1105 100-101 -1.538201080717819 1106 1 0.2225357693020733 1106 2 0.25862786448265496 1106 3 0.12790629174433832 1106 4 0.13472594634869495 1106 5 0.17389213815039284 1106 6 0.1482139465957104 1106 7 0.0954883010589711 1106 8 0.16424966203170754 1106 9 0.0679514688905698 1106 10-11 0.08298713284639092 1106 12-13 -0.0104002929726974 1106 14-15 0.259145040261501 1106 16-17 0.22717946883640394 1106 18-19 0.18179944133844828 1106 20-21 0.3493267287977915 1106 22-23 0.4038312667306556 1106 24-25 0.29146216361880306 1106 26-27 0.21971011615048752 1106 28-29 0.1773703482152058 1106 30-31 0.2135279454982424 1106 32-33 0.3030715100827308 1106 34-35 0.18397837012338414 1106 36-37 0.15425009544038204 1106 38-39 0.19520105883048444 1106 40-41 0.29112274349822087 1106 42-43 0.31432232069280275 1106 44-45 0.11843073549282224 1106 46-47 -0.12855658657773006 1106 48-49 -0.1323538487943381 1106 50-51 -0.32846530173440414 1106 52-53 -0.2632220801153622 1106 54-55 -0.18207942523145704 1106 56-57 -0.13727891642881218 1106 58-59 -0.11477569059466575 1106 60-61 -0.4950852112051365 1106 62-63 -0.420911594083929 1106 64-65 -0.683959450725272 1106 66-67 -0.8048504325576467 1106 68-69 -0.8589329543354225 1106 70-71 -1.671222366927541 1106 72-73 -1.6826159266859442 1106 74-75 -1.3947496781649562 1106 76-77 -1.5191457818488807 1106 78-79 -1.5564027719891378 1106 80-81 -1.6421846156225541 1106 82-83 -1.742359444442993 1106 84-85 -1.7770967891947826 1106 86-87 -2.0574994841268825 1106 88-89 -2.4291644359854914 1106 90-91 -2.5934771326957495 1106 92-93 -2.339750370951119 1106 94-95 -2.441546587830125 1106 96-97 -2.680983491247879 1106 98-99 -2.6759159285066225 1106 100-101 -2.400459381214935 1107 1 -0.024701671029877303 1107 2 -0.07592044130949915 1107 3 -0.08857829789348415 1107 4 -0.07507865852524276 1107 5 -0.09063282720364185 1107 6 -0.0731828732696016 1107 7 -0.07443191192253096 1107 8 -0.12348708538099373 1107 9 5.549407807379225E-4 1107 10-11 -0.002155320817159634 1107 12-13 -0.13311943375281743 1107 14-15 -0.1319258239020087 1107 16-17 -0.1338496986069302 1107 18-19 -0.058028625538391054 1107 20-21 -0.11570718777635136 1107 22-23 -0.08045547217249549 1107 24-25 0.03763844470899613 1107 26-27 -0.05467881821262921 1107 28-29 -0.14217382437436044 1107 30-31 -0.21264576247073563 1107 32-33 -0.15508082679760093 1107 34-35 -0.06312332286881173 1107 36-37 -0.2022568621173022 1107 38-39 -0.15282067695250845 1107 40-41 -0.22015036394073206 1107 42-43 -0.19140135445368855 1107 44-45 -0.33651178462613984 1107 46-47 -0.5446586523685895 1107 48-49 -0.8623782225330956 1107 50-51 -0.8336956264744586 1107 52-53 -0.7638476322220811 1107 54-55 -0.8149340297001757 1107 56-57 -1.0638629055366096 1107 58-59 -0.7383807347226785 1107 60-61 -1.1571239741619124 1107 62-63 -1.0345614956164546 1107 64-65 -1.6028109066661393 1107 66-67 -1.8701514877399497 1107 68-69 -1.1831639557860996 1107 70-71 -1.56646847482844 1107 72-73 -1.5818751919484022 1107 74-75 -1.8998194360889542 1107 76-77 -2.0986400318346945 1107 78-79 -1.996645070860204 1107 80-81 -2.0786107933826834 1107 82-83 -1.9858744698500317 1107 84-85 -2.077664699334097 1107 86-87 -2.3745085244944946 1107 88-89 -2.456635037569079 1107 90-91 -2.8588662210409304 1107 92-93 -2.571354580334301 1107 94-95 -2.674439890421075 1107 96-97 -2.8570903385868256 1107 98-99 -2.6289934422637042 1107 100-101 -2.028425561892618 1108 1 -0.07379868946187074 1108 2 -0.08376312658251805 1108 3 -0.05553546722136815 1108 4 0.016893361014972186 1108 5 0.006773926761589166 1108 6 -0.033857737844527946 1108 7 -0.0486350982904753 1108 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#Quality Count 2 3.0 3 10.0 4 38.0 5 44.0 6 136.0 7 342.0 8 776.0 9 1451.0 10 2391.0 11 4008.0 12 5970.0 13 8680.0 14 12021.0 15 16552.0 16 21342.0 17 27089.0 18 28576.0 19 26700.0 20 27777.0 21 30494.0 22 32761.0 23 35762.0 24 39407.0 25 42887.0 26 47751.0 27 54974.0 28 63341.0 29 71369.0 30 80502.0 31 90533.0 32 99939.0 33 111206.0 34 121377.0 35 123827.0 36 105622.0 37 61038.0 38 12589.0 39 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70230614065024 19.48906316184562 15.481644459990735 22.326986237513403 2 17.00325543376455 20.504975392667184 38.59760908683238 23.89416008673588 3 20.346690469027603 23.72532964756234 30.09939093958779 25.828588943822268 4 14.127754976072989 17.24944040241363 35.49100919123516 33.131795430278224 5 13.917362666053675 37.06420221868429 33.8561487750127 15.16228634024934 6 33.096766869816314 35.862983958509226 16.235251548454382 14.804997623220077 7 28.936604954256666 32.99962043143111 19.614329955054824 18.449444659257395 8 27.0273395790691 34.81622850737321 18.676547274023676 19.479884639534017 9 26.42222923813139 16.59090667224786 19.748562428387473 37.23830166123328 10-11 25.72439065055215 26.317581828952925 26.1644418745721 21.793585645922832 12-13 26.57351687068903 23.571642428516494 27.03478431144403 22.820056389350444 14-15 22.915228503825837 25.102040092373514 25.701652010117098 26.281079393683555 16-17 22.285295889285493 28.24433844662825 26.683282924063988 22.787082740022264 18-19 22.19975948832738 26.113005012433533 29.647568810105746 22.03966668913334 20-21 22.56464194622897 25.229958247457425 30.232530090564353 21.97286971574926 22-23 22.482094650921074 25.191965916870934 30.204825132405578 22.12111429980241 24-25 22.500032814136826 25.488105850955705 30.1195108600603 21.892350474847166 26-27 21.80479618519136 25.91274050998939 30.021678274537074 22.260785030282175 28-29 21.89758027438315 26.295430163982815 29.907990259795547 21.898999301838494 30-31 22.089514963799747 25.74625661117108 29.78233021852827 22.381898206500896 32-33 21.844419455194945 25.710946119000067 29.848562783124454 22.596071642680535 34-35 22.748539090504018 25.930008315618892 29.45999562933342 21.861456964543677 36-37 22.816124981376245 25.42511830520259 29.53642806972735 22.222328643693817 38-39 22.160508476800942 25.825304574893515 29.44338080171583 22.570806146589714 40-41 22.686008154102826 25.686807388143595 29.464030743372792 22.163153714380783 42-43 22.397238090204084 25.77395803142978 29.220350070363853 22.608453808002288 44-45 22.42672816389138 25.988962709680667 29.364676485080288 22.219632641347662 46-47 22.89699203898139 26.48526810704275 28.44469607156616 22.173043782409703 48-49 22.825071320477083 26.406632786631697 28.308393928837738 22.459901964053486 50-51 22.891087563456278 26.69601648887675 28.216945087925303 22.19595085974167 52-53 23.269221559010564 26.412218073453893 28.095289620491172 22.22327074704437 54-55 22.759762536070493 26.626852836646698 28.183232072246707 22.430152555036102 56-57 22.81085242714313 26.77325849553315 27.93116294251634 22.48472613480738 58-59 22.8903966397074 26.418696772481255 27.911959084931976 22.77894750287937 60-61 22.52901217246567 26.663776653542175 28.28316547252602 22.52404570146614 62-63 22.518058002751896 26.698452819986247 28.360200651571198 22.423288525690655 64-65 22.25079637439425 26.71397013480708 28.206094384114667 22.82913910668401 66-67 22.207396185715325 26.722982741523765 28.077406811501394 22.99221426125952 68-69 22.78916126944384 26.30509177053038 28.206397465540807 22.69934949448497 70-71 22.711685926726975 26.564443058377655 27.659270250027763 23.064600764867606 72-73 22.67470302658349 26.657667740979935 27.67095150335863 22.99667772907794 74-75 22.634813182758386 27.077448173338585 27.353691463280505 22.934047180622525 76-77 22.643768820311728 26.621052635313763 27.81210538771394 22.923073156660568 78-79 22.673925555114778 26.83697590629065 27.768654688057747 22.720443850536824 80-81 22.812593794635603 26.873675344791742 27.52480080946323 22.78893005110943 82-83 22.77580298486489 26.961157438014038 27.491743307439236 22.771296269681837 84-85 22.769326296181312 27.00109105424225 27.353528185567928 22.876054464008515 86-87 22.95356421881781 26.915656502751673 27.116074726758065 23.01470455167245 88-89 22.694749092560592 27.22144360037894 26.798468618385808 23.28533868867466 90-91 22.729930778412857 27.07760567398435 26.936735415137914 23.25572813246488 92-93 22.82346203712443 26.600198842850993 27.12551440475236 23.450824715272223 94-95 22.6980300329067 26.404903186894085 27.243128176472155 23.65393860372706 96-97 22.838533563758247 26.17566197786743 27.136861682255592 23.848942776118736 98-99 22.575159827719553 26.515188068089092 26.71400594608786 24.195646158103497 100-101 23.376898441937453 25.61045136877269 26.68332450536472 24.329325683925138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 694.0 1 485.0 2 434.5 3 592.5 4 471.5 5 419.0 6 841.5 7 1880.5 8 2983.5 9 3323.0 10 2614.5 11 1779.0 12 1578.0 13 1675.0 14 1881.5 15 2039.5 16 2171.0 17 2339.0 18 2548.5 19 2810.0 20 3103.5 21 3602.5 22 4262.0 23 5035.5 24 6089.0 25 7578.0 26 9543.5 27 11492.5 28 13096.0 29 14871.5 30 16697.0 31 19400.5 32 23114.0 33 24556.0 34 24620.0 35 25763.5 36 27768.5 37 30013.5 38 31994.5 39 33640.5 40 35378.5 41 38010.0 42 40059.5 43 41295.5 44 42910.5 45 45345.5 46 46799.5 47 48680.5 48 50155.0 49 49194.5 50 48429.0 51 47883.5 52 47675.0 53 48941.5 54 50486.5 55 48257.0 56 44376.5 57 41274.5 58 38539.0 59 34770.0 60 29266.0 61 24992.5 62 21613.0 63 17442.5 64 13476.5 65 10447.5 66 7962.5 67 6322.5 68 4987.5 69 3821.5 70 2973.0 71 2190.5 72 1619.0 73 1267.5 74 995.5 75 724.0 76 524.0 77 371.5 78 236.5 79 144.0 80 90.0 81 52.5 82 20.5 83 14.0 84 10.0 85 5.0 86 4.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005959581268468494 2 0.01128063597245822 3 0.007875160961904796 4 0.0 5 7.8042135658516E-4 6 0.001773684901329909 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.385265644787672E-4 14-15 0.0 16-17 0.0022703166737022837 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002944316936207649 26-27 0.01773684901329909 28-29 0.0056048442882025125 30-31 0.0030862117283140416 32-33 0.0 34-35 0.0070237922092664384 36-37 3.547369802659818E-4 38-39 0.0021638955796224888 40-41 0.0014544216190905254 42-43 0.0025186325598884705 44-45 3.547369802659818E-5 46-47 1.4189479210639273E-4 48-49 8.513687526383562E-4 50-51 0.004256843763191781 52-53 0.007626845075718609 54-55 0.007236634397426028 56-57 0.015963164111969182 58-59 0.02355453548966119 60-61 0.0030862117283140416 62-63 0.020290955271214158 64-65 0.021000429231746123 66-67 0.013302636759974316 68-69 0.031004012075246806 70-71 0.016424322186314958 72-73 0.004788949233590754 74-75 0.016317901092235163 76-77 0.019829797196868382 78-79 0.02639243133178905 80-81 0.011777267744830596 82-83 0.034515908179880025 84-85 0.02181632428635788 86-87 0.03132327535748619 88-89 0.02199369277649087 90-91 0.028166116233118956 92-93 0.024264009450193153 94-95 0.014721584681038244 96-97 0.015218216453410618 98-99 0.0117063203487774 100-101 0.01053568831389966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1409495.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.77127979322964 #Duplication Level Percentage of deduplicated Percentage of total 1 82.13266753604522 49.91307318997313 2 10.666178094623563 12.96394586625571 3 3.0381420306331157 5.538953381855278 4 1.2975758334820717 3.154213761178885 5 0.6982913954939244 2.1218030886383024 6 0.45008018982441617 1.6411169487125705 7 0.3102588961047024 1.3198381128462204 8 0.21893634037059556 1.0644033278053777 9 0.15604533187781228 0.8534767071576516 >10 0.9290753437398118 10.350204905599028 >50 0.06262093148279362 2.6210781662084655 >100 0.03382896437859014 3.9063399717858043 >500 0.004666008846992138 1.9876800075670011 >1k 0.0013998026540976414 1.530582368963912 >5k 2.333004423496069E-4 1.0332901954526412 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7749 0.5497713720162185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6827 0.4843578728551715 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4399 0.3120975952380108 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1857 0.13174931447078564 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1853 0.13146552488657284 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1817 0.1289114186286578 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1761 0.12493836444967879 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1702 0.12075246808254021 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1602 0.11365772847722055 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1467 0.10407983001003906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.094739605319635E-5 2 0.0 0.0 0.0 0.0 7.094739605319635E-5 3 0.0 0.0 0.0 0.0 7.094739605319635E-5 4 0.0 0.0 0.0 0.0 7.094739605319635E-5 5 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 6 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 7 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 8 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 9 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 10-11 7.094739605319635E-5 0.0 0.0 0.0 7.094739605319635E-5 12-13 1.773684901329909E-4 0.0 0.0 0.0 1.418947921063927E-4 14-15 2.837895842127854E-4 0.0 0.0 0.0 1.418947921063927E-4 16-17 3.1926328223938363E-4 0.0 0.0 0.0 2.1284218815958907E-4 18-19 3.547369802659818E-4 0.0 0.0 0.0 2.1284218815958907E-4 20-21 3.547369802659818E-4 0.0 0.0 3.5473698026598176E-5 2.1284218815958907E-4 22-23 3.547369802659818E-4 0.0 0.0 7.094739605319635E-5 2.8378958421278546E-4 24-25 4.2568437631917814E-4 0.0 0.0 7.094739605319635E-5 3.547369802659818E-4 26-27 4.966317723723745E-4 0.0 0.0 2.4831588618618724E-4 3.547369802659818E-4 28-29 7.094739605319636E-4 0.0 0.0 0.0016317901092235162 3.547369802659818E-4 30-31 7.8042135658516E-4 0.0 0.0 0.006172423456628083 3.547369802659818E-4 32-33 7.8042135658516E-4 0.0 0.0 0.016495269582368154 3.9021067829258E-4 34-35 8.158950546117581E-4 0.0 0.0 0.0330614865607895 4.2568437631917814E-4 36-37 8.513687526383563E-4 0.0 0.0 0.061192129095881856 4.2568437631917814E-4 38-39 8.513687526383563E-4 0.0 0.0 0.10383151412385287 4.2568437631917814E-4 40-41 8.513687526383563E-4 0.0 0.0 0.14945068978605813 4.2568437631917814E-4 42-43 9.93263544744749E-4 0.0 0.0 0.19581481310682194 4.2568437631917814E-4 44-45 0.0010642109407979453 0.0 0.0 0.2528210458355652 4.2568437631917814E-4 46-47 0.0012770531289575345 0.0 0.0 0.3193342296354368 4.2568437631917814E-4 48-49 0.0013480005250107309 0.0 0.0 0.3914167840254843 4.966317723723745E-4 50-51 0.0013480005250107309 0.0 0.0 0.47258060511034095 4.966317723723745E-4 52-53 0.0013480005250107309 0.0 0.0 0.5456564230451332 4.966317723723745E-4 54-55 0.0013480005250107309 0.0 0.0 0.6239823482878619 4.966317723723745E-4 56-57 0.0013480005250107309 0.0 0.0 0.7230603868761507 4.966317723723745E-4 58-59 0.0013480005250107309 0.0 0.0 0.8299781127283176 4.966317723723745E-4 60-61 0.0013480005250107309 0.0 0.0 0.941081734947623 5.675791684255708E-4 62-63 0.0013480005250107309 0.0 0.0 1.0442747225069973 5.675791684255708E-4 64-65 0.0013480005250107309 0.0 0.0 1.1547043444637972 5.675791684255708E-4 66-67 0.0014189479210639272 0.0 0.0 1.283544815696402 5.675791684255708E-4 68-69 0.0014189479210639272 0.0 0.0 1.424162554673837 5.675791684255708E-4 70-71 0.0014189479210639272 0.0 0.0 1.5716621910684323 5.675791684255708E-4 72-73 0.0014544216190905254 0.0 0.0 1.7221416180972617 5.675791684255708E-4 74-75 0.00156084271317032 0.0 0.0 1.8765586256070437 5.675791684255708E-4 76-77 0.00156084271317032 0.0 0.0 2.0422207953912572 5.675791684255708E-4 78-79 0.00156084271317032 0.0 0.0 2.2218241284999234 5.675791684255708E-4 80-81 0.002199369277649087 0.0 0.0 2.41884504733965 5.675791684255708E-4 82-83 0.002234842975675685 0.0 0.0 2.620051862546515 5.675791684255708E-4 84-85 0.0023057903717288815 0.0 0.0 2.8202299405106084 6.030528664521691E-4 86-87 0.0023412640697554796 0.0 0.0 3.0228557036385375 6.385265644787673E-4 88-89 0.0023412640697554796 0.0 0.0 3.229986626415844 6.385265644787673E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4190 0.0 47.838703 1 GTATCAA 7040 0.0 38.727566 1 TATCAAC 8615 0.0 31.371695 2 ATCAACG 8755 0.0 30.812498 3 TCAACGC 8955 0.0 30.122198 4 CAACGCA 9075 0.0 29.723888 5 AACGCAG 9365 0.0 28.854156 6 ACGCAGA 10510 0.0 25.529026 7 CGCAGAG 10525 0.0 25.447523 8 GTACCGT 135 1.3451592E-5 24.624544 6 GAGTACT 7335 0.0 24.021114 12-13 GCAGAGT 11175 0.0 23.584894 9 TACATGG 4825 0.0 23.13404 2 GTATAGG 250 3.8380676E-10 22.799335 1 GTACATG 5025 0.0 22.780432 1 GTACTTT 7700 0.0 22.697416 14-15 ACATGGG 4950 0.0 21.779877 3 AGAGTAC 9630 0.0 21.156443 10-11 CAGAGTA 10735 0.0 21.14645 10-11 GTATAGC 540 0.0 21.110497 1 >>END_MODULE