FastQCFastQC Report
Thu 26 May 2016
SRR937919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505437
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134990.5387882433284094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109470.43692976514675885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68830.2747225334342871No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38420.15334650202739084No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36300.1448849043101064No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33190.13247190011163723No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33140.13227233412773898No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31630.1262454414140128No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.11000077032469785No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27480.1096814647504607No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26560.10600945064673348No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25830.10309578728181949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.059.4892271
GTATCAA102850.043.442241
TATCAAC132400.033.2005232
ATCAACG132500.033.0678673
TCAACGC133400.032.9872634
CAACGCA136100.032.332855
AACGCAG139750.031.5532386
TGGTATC21300.028.5575872
ACGCAGA157000.027.8056817
CGCAGAG158500.027.4831478
GCAGAGT173250.024.8397169
GTGGTAT26050.024.711721
GAGTACT112500.024.1728412-13
CAGAGTA164750.022.64754510-11
GTACTTT118650.022.63941614-15
GTGTAAG11300.022.365281
TACATGG95950.022.2873612
TAAGGTG17300.022.2500025
GTACATG100800.021.7607671
AGAGTAC158750.021.6633310-11
Produced by FastQC (version 0.11.5)