FastQCFastQC Report
Thu 26 May 2016
SRR937919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505437
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128610.5133236237829968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110460.44088117162794355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69900.278993245489709No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37930.15139075538518829No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36020.14376733480027634No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34920.13937688315451555No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34470.1375807892994316No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33710.13454738634417868No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.11435130877367901No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28110.11219599614757825No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27910.11139773221198537No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25380.10129969342673553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60050.057.180431
GTATCAA104850.041.9562381
ATCAACG134200.032.561133
TATCAAC135750.032.335912
TCAACGC136100.032.1065674
CAACGCA137900.031.687485
AACGCAG141850.030.8376396
TGGTATC24400.029.6262722
GTGGTAT29300.026.7817881
ACGCAGA162900.026.7356327
CGCAGAG164000.026.5273448
TAAGGTG19000.024.0244855
GCAGAGT180100.023.7603729
GAGTACT113200.023.28566712-13
GTGTAAG10100.023.0726761
GTAAGGT19100.022.4050334
CAGAGTA170800.022.07876610-11
GTACTTT118950.021.98037114-15
GGTAAGG19450.021.7573953
GTACATG99150.021.4406551
Produced by FastQC (version 0.11.5)