##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937919_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2505437 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48212148220051 33.0 31.0 34.0 31.0 34.0 2 32.71467492497317 34.0 31.0 34.0 31.0 34.0 3 32.77122553869844 34.0 31.0 34.0 31.0 34.0 4 36.23203417208256 37.0 35.0 37.0 35.0 37.0 5 36.15817360404592 37.0 35.0 37.0 35.0 37.0 6 36.21161418147812 37.0 37.0 37.0 35.0 37.0 7 36.18500684710891 37.0 37.0 37.0 35.0 37.0 8 36.17920067437337 37.0 37.0 37.0 35.0 37.0 9 37.87143320706128 39.0 38.0 39.0 35.0 39.0 10-11 37.844642471552866 39.0 38.0 39.0 35.0 39.0 12-13 37.76972280683968 39.0 38.0 39.0 35.0 39.0 14-15 39.00473290687413 40.0 38.0 41.0 36.0 41.0 16-17 38.917923899104224 40.0 38.0 41.0 35.5 41.0 18-19 39.17879475716212 40.0 39.0 41.0 36.0 41.0 20-21 39.154043985141115 40.0 39.0 41.0 36.0 41.0 22-23 39.05314342368217 40.0 39.0 41.0 35.5 41.0 24-25 38.94252479707133 40.0 38.0 41.0 35.0 41.0 26-27 38.7764413952536 40.0 38.0 41.0 35.0 41.0 28-29 38.65326288388014 40.0 38.0 41.0 34.5 41.0 30-31 38.396850130336546 40.0 38.0 41.0 34.0 41.0 32-33 38.14745311895689 40.0 38.0 41.0 34.0 41.0 34-35 38.02764188442974 40.0 37.5 41.0 33.0 41.0 36-37 37.953039529630956 40.0 37.5 41.0 33.0 41.0 38-39 37.748695536946244 40.0 37.0 41.0 33.0 41.0 40-41 37.51368224385606 40.0 37.0 41.0 33.0 41.0 42-43 37.24051213421052 40.0 36.5 41.0 32.0 41.0 44-45 36.94174249841444 40.0 36.0 41.0 31.0 41.0 46-47 36.5400624721356 39.0 36.0 41.0 30.5 41.0 48-49 36.08532683120749 39.0 35.0 41.0 29.5 41.0 50-51 35.685919063221306 39.0 35.0 40.0 28.0 41.0 52-53 35.143300150831976 38.0 34.5 40.0 26.5 41.0 54-55 34.75962975720403 38.0 34.0 40.0 26.0 41.0 56-57 34.380983636786716 38.0 34.0 40.0 25.0 41.0 58-59 33.87327340499881 37.5 33.0 40.0 23.0 41.0 60-61 33.393638914089635 37.0 33.0 39.5 22.0 41.0 62-63 32.84522280943405 36.0 32.0 39.0 19.5 40.5 64-65 32.27731130337742 35.5 31.0 39.0 17.5 40.0 66-67 31.718484639605784 35.0 31.0 38.5 12.5 40.0 68-69 31.097403167591125 35.0 30.0 38.0 8.0 40.0 70-71 30.480174915593565 34.5 29.0 37.0 6.5 39.5 72-73 29.81970430707298 34.0 29.0 36.5 2.0 39.0 74-75 29.173387516828402 34.0 28.0 36.0 2.0 39.0 76-77 27.88642620029959 32.0 26.5 34.5 2.0 37.0 78-79 28.530814384875775 33.5 28.0 35.0 2.0 37.0 80-81 28.257846236005932 34.0 27.0 35.0 2.0 37.0 82-83 27.69095970084261 33.0 26.0 35.0 2.0 36.5 84-85 27.036761451195936 33.0 25.5 35.0 2.0 36.0 86-87 26.32187658280771 32.5 24.5 35.0 2.0 35.5 88-89 25.50193299612004 32.0 21.0 34.5 2.0 35.0 90-91 24.834452233283056 31.0 18.5 34.0 2.0 35.0 92-93 23.896151848958883 31.0 8.5 34.0 2.0 35.0 94-95 23.001413326297968 30.5 2.0 34.0 2.0 35.0 96-97 21.95171281497 30.0 2.0 34.0 2.0 35.0 98-99 20.522506852098054 29.0 2.0 34.0 2.0 35.0 100-101 16.683357633817973 20.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19078020123298955 1101 2 0.15777364734647392 1101 3 0.15626996068907317 1101 4 0.030511811055788485 1101 5 -0.09064857461787312 1101 6 0.006391910021172009 1101 7 0.06023522548271387 1101 8 0.013229019998107105 1101 9 0.04492165851771546 1101 10-11 0.0974554006602304 1101 12-13 0.1678899077440974 1101 14-15 0.3460211552595496 1101 16-17 0.3965707470664839 1101 18-19 0.28257217005351265 1101 20-21 0.24075468303066572 1101 22-23 0.22786247153073447 1101 24-25 0.3539192612260109 1101 26-27 0.4950874843622657 1101 28-29 0.41224217029623134 1101 30-31 0.4827841203523846 1101 32-33 0.462124512838777 1101 34-35 0.4021563573234417 1101 36-37 0.4984595762967814 1101 38-39 0.587657211776893 1101 40-41 0.7076314449128631 1101 42-43 0.812341453068079 1101 44-45 0.756094682114977 1101 46-47 0.8002324727385783 1101 48-49 0.954516200240441 1101 50-51 0.8385440035084173 1101 52-53 0.9594978872112492 1101 54-55 1.046420244415465 1101 56-57 1.1092770925761712 1101 58-59 1.2435524835271963 1101 60-61 1.033991340336037 1101 62-63 1.1180521352952582 1101 64-65 1.4001674663693038 1101 66-67 1.5902633481025354 1101 68-69 1.7412317053682962 1101 70-71 1.538516427907961 1101 72-73 1.7286150141480299 1101 74-75 1.8662466729540874 1101 76-77 1.9499305191213203 1101 78-79 1.889252446680029 1101 80-81 1.9429504293364595 1101 82-83 2.1101389448695684 1101 84-85 2.3338315348676844 1101 86-87 2.341095929640538 1101 88-89 2.7585730073537746 1101 90-91 3.028641967226079 1101 92-93 3.2929682113984065 1101 94-95 3.6827706479428386 1101 96-97 3.8355431560619913 1101 98-99 4.170544879900792 1101 100-101 4.4157194914847615 1102 1 0.21991629546297986 1102 2 0.2171937429356703 1102 3 0.17214087599239036 1102 4 0.11036029926764712 1102 5 -0.08534361296112536 1102 6 0.023193445459014583 1102 7 0.10231163537221732 1102 8 0.13434133304163964 1102 9 0.09266695376335576 1102 10-11 0.09968792848065533 1102 12-13 0.21784510564175008 1102 14-15 0.33235107119637064 1102 16-17 0.35627489524170386 1102 18-19 0.34536032078324297 1102 20-21 0.17275196459533504 1102 22-23 0.25157738330965174 1102 24-25 0.3021861733259499 1102 26-27 0.3181880160964141 1102 28-29 0.2888227304100184 1102 30-31 0.3738119177196708 1102 32-33 0.2887364280208615 1102 34-35 0.45904531555823525 1102 36-37 0.6041735412337488 1102 38-39 0.682475343842988 1102 40-41 0.7273698766215659 1102 42-43 0.783682194934542 1102 44-45 0.7479795037859631 1102 46-47 0.7669874558251948 1102 48-49 0.9674258865446177 1102 50-51 1.007387631151083 1102 52-53 1.1260290518866185 1102 54-55 1.2854095781426835 1102 56-57 1.3372853221610157 1102 58-59 1.4076847400163501 1102 60-61 1.3686494911169902 1102 62-63 1.4750131525569827 1102 64-65 1.600066165379431 1102 66-67 1.6529772199774833 1102 68-69 1.8711781861727594 1102 70-71 1.9130295598972218 1102 72-73 1.999930911018243 1102 74-75 2.100726447761094 1102 76-77 2.0072794512987464 1102 78-79 2.1930767618204534 1102 80-81 2.423431419072319 1102 82-83 2.400683460101856 1102 84-85 2.5019448604942305 1102 86-87 2.705050578557689 1102 88-89 2.9973197608555537 1102 90-91 3.022844374543997 1102 92-93 3.392835704751729 1102 94-95 3.569595286665855 1102 96-97 3.777493483998864 1102 98-99 4.059024997927331 1102 100-101 3.946328996446077 1103 1 0.16614041905879162 1103 2 0.20094263420944714 1103 3 0.1526019754241048 1103 4 0.09539065753936171 1103 5 0.02888050736772385 1103 6 0.10553321307980212 1103 7 0.13951950745094166 1103 8 0.03182172716829257 1103 9 0.20480001234493983 1103 10-11 0.2356854513044837 1103 12-13 0.23351193810688642 1103 14-15 0.39563851183970655 1103 16-17 0.3883548574755835 1103 18-19 0.30481473355446553 1103 20-21 0.27306167287169814 1103 22-23 0.31519978995203957 1103 24-25 0.31775162778528454 1103 26-27 0.3962593919296893 1103 28-29 0.39474467608365416 1103 30-31 0.31544268860904623 1103 32-33 0.4042780540546502 1103 34-35 0.4648804212200517 1103 36-37 0.5142321587521934 1103 38-39 0.4298085816175998 1103 40-41 0.4982914180083 1103 42-43 0.5817678351534923 1103 44-45 0.5751243887806226 1103 46-47 0.6751537859944747 1103 48-49 0.6771079659351287 1103 50-51 0.7939409872224985 1103 52-53 0.8723150232124723 1103 54-55 0.9168515225422098 1103 56-57 0.8036134291671786 1103 58-59 1.0154614029423428 1103 60-61 0.9811113901383735 1103 62-63 1.0374770117573249 1103 64-65 1.1174429477129877 1103 66-67 1.2728624800850703 1103 68-69 1.297068617885845 1103 70-71 1.390983263737045 1103 72-73 1.6059855771425937 1103 74-75 1.7088452231719522 1103 76-77 1.6549569863410305 1103 78-79 1.629702031957617 1103 80-81 1.7681204162610946 1103 82-83 2.031833113231855 1103 84-85 2.4374506846759765 1103 86-87 2.6262891584733694 1103 88-89 2.896882640946508 1103 90-91 3.114534604195299 1103 92-93 3.09340121638294 1103 94-95 3.187375089961897 1103 96-97 3.3625800544860844 1103 98-99 3.7885831899235924 1103 100-101 3.6837848720008495 1104 1 0.24498393534516794 1104 2 0.16649216637186015 1104 3 0.20009626524352342 1104 4 0.14686357237409453 1104 5 0.06362563781080155 1104 6 0.08206645411011948 1104 7 0.11597116753576842 1104 8 0.1391261159706474 1104 9 0.18824218825637473 1104 10-11 0.23336301660274472 1104 12-13 0.23989758949480233 1104 14-15 0.37083557352713825 1104 16-17 0.43066176460403227 1104 18-19 0.3185977371745139 1104 20-21 0.35971491364562524 1104 22-23 0.36315637179589544 1104 24-25 0.4224289159025716 1104 26-27 0.4029336844723588 1104 28-29 0.4255528460657203 1104 30-31 0.3781828060972643 1104 32-33 0.5072048481701827 1104 34-35 0.5046800739571538 1104 36-37 0.5849742986708932 1104 38-39 0.6863692921177105 1104 40-41 0.694127060373745 1104 42-43 0.8133615713684961 1104 44-45 0.8414982187010622 1104 46-47 0.9979461813295174 1104 48-49 0.9502917652801912 1104 50-51 1.0811787554708445 1104 52-53 1.1217663427939684 1104 54-55 1.214928193480219 1104 56-57 1.2316977861957028 1104 58-59 1.449530693524153 1104 60-61 1.4217294939990737 1104 62-63 1.5152310533647722 1104 64-65 1.664405213223617 1104 66-67 1.772906961124388 1104 68-69 1.8604524478820288 1104 70-71 1.965298066347092 1104 72-73 2.089620342357488 1104 74-75 2.128457787492213 1104 76-77 2.1388437784053806 1104 78-79 2.2515699792120394 1104 80-81 2.338240889951141 1104 82-83 2.450498755863414 1104 84-85 2.671882272351894 1104 86-87 2.630090733412942 1104 88-89 2.852953341324749 1104 90-91 3.1097136559178082 1104 92-93 3.4636572115626727 1104 94-95 3.622689558284307 1104 96-97 3.976770554928855 1104 98-99 4.210069590865579 1104 100-101 3.7739057885588885 1105 1 0.27472884779017903 1105 2 0.22951350478875554 1105 3 0.20869394740545744 1105 4 0.12123350463835436 1105 5 0.07254190286882078 1105 6 0.07351283520753782 1105 7 0.16879121613441583 1105 8 0.11966103287729624 1105 9 0.29786789022650595 1105 10-11 0.21705242040288653 1105 12-13 0.28489640955815076 1105 14-15 0.3630411701141725 1105 16-17 0.32540339535022866 1105 18-19 0.3428249708499109 1105 20-21 0.30518165233758765 1105 22-23 0.3254405263528426 1105 24-25 0.3692580205150122 1105 26-27 0.4559546910320691 1105 28-29 0.5057558136063989 1105 30-31 0.5594269755509202 1105 32-33 0.6119879241912898 1105 34-35 0.7168575733026685 1105 36-37 0.7051309352614723 1105 38-39 0.6279267531890227 1105 40-41 0.619855302526247 1105 42-43 0.6815379423995793 1105 44-45 0.8721197872056052 1105 46-47 0.7233136209776063 1105 48-49 0.7654355147501448 1105 50-51 0.9068275362644158 1105 52-53 0.9405702004493648 1105 54-55 1.028486495256395 1105 56-57 1.1279435596257414 1105 58-59 1.2757663084370776 1105 60-61 1.230090543552727 1105 62-63 1.3569618176318627 1105 64-65 1.390221674077381 1105 66-67 1.6672168088019497 1105 68-69 1.7795299955311883 1105 70-71 1.9992810715720033 1105 72-73 2.119108249850452 1105 74-75 2.0507977678020204 1105 76-77 1.935033412661891 1105 78-79 2.1898739493301456 1105 80-81 2.3860794856790335 1105 82-83 2.519875528072955 1105 84-85 2.75723262135503 1105 86-87 2.860108874938973 1105 88-89 2.9117271843415544 1105 90-91 3.1403976320093463 1105 92-93 3.331971026777218 1105 94-95 3.6025932090820554 1105 96-97 3.83845312615831 1105 98-99 3.8537232831454773 1105 100-101 3.586886843201018 1106 1 0.1863196910240248 1106 2 0.13943333478972164 1106 3 0.12713787670131893 1106 4 0.1593335722516116 1106 5 0.06017350723470116 1106 6 0.12714463275413834 1106 7 0.11776252879740667 1106 8 0.11662809556443676 1106 9 0.20957947141069866 1106 10-11 0.18812119273940908 1106 12-13 0.17523737352994573 1106 14-15 0.07956536599942154 1106 16-17 0.03993300029565461 1106 18-19 0.05531410443438034 1106 20-21 0.06480929649870149 1106 22-23 0.03309531240520158 1106 24-25 0.0923213837913579 1106 26-27 -0.07481416823577547 1106 28-29 0.15935863404492068 1106 30-31 0.23109943763920882 1106 32-33 0.21497015156499089 1106 34-35 0.17193098197648027 1106 36-37 0.40112002055075635 1106 38-39 0.3158457138767403 1106 40-41 0.41655432696360606 1106 42-43 0.2750349963935932 1106 44-45 0.38577041987245764 1106 46-47 0.4027905841766781 1106 48-49 0.500039011321185 1106 50-51 0.5349474049015299 1106 52-53 0.5014800816432157 1106 54-55 0.6968096866018954 1106 56-57 0.6839901161793946 1106 58-59 0.8167785754128332 1106 60-61 0.6756280689076917 1106 62-63 0.7778374092478231 1106 64-65 0.8955993423860775 1106 66-67 0.9922989106081879 1106 68-69 1.1439171296952004 1106 70-71 1.146141884862832 1106 72-73 1.4868567285522793 1106 74-75 1.615035186907594 1106 76-77 1.6082658090687296 1106 78-79 1.7416777743084104 1106 80-81 1.6004255689399223 1106 82-83 2.0351706116236166 1106 84-85 2.3451008721036075 1106 86-87 2.3245443042831226 1106 88-89 2.3650305746632014 1106 90-91 2.5019281062680143 1106 92-93 2.44995856662074 1106 94-95 2.723109001605959 1106 96-97 2.8838240331417957 1106 98-99 3.0228699073123835 1106 100-101 3.0001897505708612 1107 1 0.20995610861557168 1107 2 0.2528772582668779 1107 3 0.23617995634995737 1107 4 0.14793329132494648 1107 5 0.03501375492497516 1107 6 0.023706248675956942 1107 7 0.10406735400958667 1107 8 0.12074802355225245 1107 9 0.1550114456129279 1107 10-11 0.19942997466514356 1107 12-13 0.17127324113830866 1107 14-15 0.2909058016335848 1107 16-17 0.2733939376410959 1107 18-19 0.21127291504628687 1107 20-21 0.24076929412468218 1107 22-23 0.21610073310277045 1107 24-25 0.27609836363190254 1107 26-27 0.31128194033644263 1107 28-29 0.39244615654872916 1107 30-31 0.4957862637614241 1107 32-33 0.40569858253023483 1107 34-35 0.4427803894586262 1107 36-37 0.512627627332563 1107 38-39 0.5313431716882846 1107 40-41 0.4537919473960841 1107 42-43 0.48714204527878735 1107 44-45 0.4166649338891162 1107 46-47 0.46766090288995343 1107 48-49 0.5137242943251223 1107 50-51 0.671651858182635 1107 52-53 0.6291063502645002 1107 54-55 0.7394704775489345 1107 56-57 0.7948770135224592 1107 58-59 0.940764667564693 1107 60-61 0.9662165311309749 1107 62-63 1.0101113325271527 1107 64-65 1.1008662227152826 1107 66-67 1.3012588884505938 1107 68-69 1.4165297476547245 1107 70-71 1.3372190012099985 1107 72-73 1.5561338369860174 1107 74-75 1.6756495656046475 1107 76-77 1.571972853997213 1107 78-79 1.4867665036098536 1107 80-81 1.6623679196234455 1107 82-83 1.8789991276582008 1107 84-85 1.8865969054982656 1107 86-87 2.1191067906819185 1107 88-89 2.317468404801488 1107 90-91 2.5365938628905624 1107 92-93 2.670060649830429 1107 94-95 2.6405966863350265 1107 96-97 2.787290114214855 1107 98-99 3.2129702100009716 1107 100-101 3.065485415991162 1108 1 0.23489843518761688 1108 2 0.17013723429738548 1108 3 0.1380091989583292 1108 4 0.12295059029466415 1108 5 0.040444032168160504 1108 6 0.10248887661273898 1108 7 0.09604734365904477 1108 8 0.1776885939227526 1108 9 0.23793498984716877 1108 10-11 0.16013741207729737 1108 12-13 0.17834244173626956 1108 14-15 0.28802269827444604 1108 16-17 0.27904181748390755 1108 18-19 0.2949189173894027 1108 20-21 0.2677180215339874 1108 22-23 0.3284639864731176 1108 24-25 0.29730622759760195 1108 26-27 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0.41554226728587196 2316 56-57 0.46568591913938207 2316 58-59 0.5467664962216929 2316 60-61 0.5732281896726263 2316 62-63 0.6209529448089981 2316 64-65 0.8674457480645117 2316 66-67 1.074957055738711 2316 68-69 1.2191220334636803 2316 70-71 1.149427778299387 2316 72-73 1.2832719041389886 2316 74-75 1.5574629478405164 2316 76-77 1.6776311780250204 2316 78-79 1.6618717428609635 2316 80-81 1.7735554257757435 2316 82-83 1.944008197900665 2316 84-85 2.178945217007815 2316 86-87 2.288602842358344 2316 88-89 2.6023039178282446 2316 90-91 2.8698457799836206 2316 92-93 2.9811389062194955 2316 94-95 3.414487016884504 2316 96-97 3.7631432815500787 2316 98-99 3.9059497044402534 2316 100-101 4.25503505220529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 12.0 5 37.0 6 113.0 7 245.0 8 635.0 9 1223.0 10 2212.0 11 3791.0 12 5879.0 13 8689.0 14 11459.0 15 16152.0 16 21468.0 17 30396.0 18 30982.0 19 24787.0 20 23321.0 21 24325.0 22 25874.0 23 28652.0 24 32138.0 25 36030.0 26 40284.0 27 47445.0 28 56422.0 29 68739.0 30 81455.0 31 98012.0 32 118942.0 33 147895.0 34 192947.0 35 263856.0 36 361559.0 37 442257.0 38 245164.0 39 12031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.164126657345605 19.066613928029323 15.150730191978484 22.61852922264659 2 15.671549143520707 20.144458402742266 40.080500391005316 24.103492062731704 3 19.34311682433422 23.920471849146853 30.075673241991126 26.660738084527797 4 12.88687013425029 17.04310098806775 36.76126918110717 33.30875969657479 5 13.367912709719057 37.56079529901636 34.34303513949302 14.72825685177156 6 32.163064632263605 36.38069381935918 16.972135787942698 14.484105760434513 7 28.456512776014726 33.39628974905376 20.01742610171399 18.129771373217526 8 26.63431027807602 34.79259049344823 19.355639550895454 19.217459677580294 9 26.231990666698064 16.80876429940166 20.164825537421216 36.79441949647906 10-11 25.013660291597834 26.80300881642604 26.755532068856652 21.427798823119478 12-13 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29.824925526443774 21.94251302027465 38-39 21.60785596518998 26.382199340546304 29.799854745354317 22.210089948909403 40-41 22.201407564090232 26.231818165488374 29.758472348682467 21.808301921738927 42-43 21.83105604887904 26.23306755534746 29.636909790556885 22.29896660521661 44-45 21.792427262048218 26.646919426550365 29.72675445021392 21.833898861187492 46-47 22.310523535519657 26.927762886826308 28.846350725711865 21.915362851942167 48-49 22.02601781645278 27.015207327105013 28.70227828518538 22.256496571256832 50-51 22.296449681233256 27.249358096012795 28.63157205709024 21.822620165663714 52-53 22.652754788885133 26.896345827095235 28.554739153289425 21.896160230730207 54-55 21.996601790426183 27.21297721714815 28.725527722309526 22.06489327011615 56-57 22.13543984542417 27.34022048848165 28.451623409409216 22.072716256684963 58-59 22.03707377196074 26.99610886244595 28.359044749478834 22.607772616114474 60-61 21.658556970300992 27.229980238976275 28.73570558748833 22.375757203234407 62-63 21.841698673724384 27.16771565200003 28.88230675925996 22.108278915015624 64-65 21.606849423872962 27.279612299171763 28.625345598392617 22.488192678562662 66-67 21.555321486830444 27.305136788512343 28.596388574128984 22.54315315052823 68-69 22.18761936654755 26.899996128382824 28.67203930050654 22.24034520456308 70-71 21.95397449626552 27.08830834700693 28.17805835868154 22.77965879804601 72-73 21.938587958906968 27.274064364819388 28.203682631014072 22.58366504525957 74-75 21.991053856073812 27.637034976333467 27.902258168934203 22.469652998658518 76-77 21.93475629201612 27.223913433065768 28.365430860963574 22.47589941395453 78-79 22.04367940602777 27.52426023883259 28.17556378388281 22.256496571256832 80-81 22.201525004031232 27.593281169425204 27.845752196424094 22.359441630119466 82-83 22.104322867678448 27.705556689778877 27.94112921943549 22.248991223107183 84-85 22.068441532082232 27.78480167626252 27.878421872040693 22.268334919614556 86-87 22.337139617250834 27.704939920159642 27.559013632492867 22.398906830096657 88-89 22.083712342397753 27.90002302991454 27.325153256697334 22.69111137099037 90-91 21.99311521468105 27.85430311056668 27.580871480747724 22.57171019400454 92-93 22.089260276750124 27.457166155046004 27.691257054158612 22.762316514045256 94-95 21.8195069363149 27.32882527080106 27.870247785116923 22.98142000776711 96-97 22.182925446908243 26.98041378825881 27.779356567080537 23.05730419775241 98-99 21.81980628529075 27.26735894776041 27.463432526940412 23.44940224000843 100-101 22.109256788336726 26.757288249514954 27.814289483231867 23.319165478916453 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 991.0 1 682.0 2 530.0 3 709.5 4 588.5 5 535.5 6 1060.5 7 2433.5 8 3944.0 9 4574.0 10 3781.0 11 2808.5 12 2752.5 13 2905.5 14 3145.0 15 3351.5 16 3563.5 17 3908.0 18 4254.0 19 4694.0 20 5298.5 21 6329.0 22 7797.0 23 9506.5 24 11678.5 25 14587.0 26 18566.0 27 23240.0 28 26806.0 29 30004.5 30 33773.5 31 39460.0 32 47804.0 33 50780.0 34 50828.0 35 53548.0 36 57026.5 37 60387.5 38 63338.0 39 65970.0 40 69615.0 41 74398.5 42 76854.0 43 78921.0 44 81225.0 45 85041.5 46 86793.0 47 89166.5 48 91152.0 49 88734.5 50 86894.0 51 83975.0 52 81287.5 53 82058.0 54 84359.0 55 80014.5 56 72340.5 57 66353.0 58 62240.5 59 55776.0 60 44782.0 61 37118.5 62 31768.5 63 23557.0 64 15906.5 65 11776.0 66 8748.0 67 6683.0 68 5262.5 69 4232.5 70 3268.0 71 2161.5 72 1543.0 73 1235.5 74 923.5 75 635.5 76 461.0 77 314.5 78 178.5 79 104.0 80 59.0 81 30.5 82 17.0 83 11.5 84 7.0 85 4.0 86 2.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01420909805355313 3 0.0023947918067786177 4 0.0016763542647450327 5 0.1190211527968973 6 3.9913196779643634E-5 7 0.0 8 2.793923774575054E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.9913196779643634E-5 18-19 0.0 20-21 0.0 22-23 3.791753694066145E-4 24-25 6.984809436437635E-4 26-27 0.007304115010674784 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011674610058045761 36-37 6.984809436437635E-4 38-39 0.007244245215505319 40-41 0.010297604769148056 42-43 0.006006936115336366 44-45 0.0043106252522015125 46-47 1.9956598389821817E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.579167227114471E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.9913196779643634E-5 82-83 0.002055529634151647 84-85 0.004051189473133828 86-87 0.002494574798727727 88-89 0.0 90-91 0.002295008814829509 92-93 0.0 94-95 0.0 96-97 3.9913196779643634E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2505437.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.976463567454765 #Duplication Level Percentage of deduplicated Percentage of total 1 73.91973271384461 37.681665616032944 2 14.544584138925694 14.828629269234522 3 4.953343844255394 7.575118560412843 4 2.229331853805525 4.545738161011349 5 1.2059295747740888 3.073701251669378 6 0.7296194279487933 2.231605091216331 7 0.46963818838803173 1.675834580017357 8 0.3164415434864354 1.2904856650212344 9 0.23654222546086512 1.0852277524533418 >10 1.2360393068694715 11.634710378398186 >50 0.09903645731129954 3.519836700124811 >100 0.052475130249583814 5.048447784255488 >500 0.003368608293024825 1.1949297670031027 >1k 0.0036819672040013735 3.3802032039433305 >5k 7.833972774471009E-5 0.2791444111806514 >10k+ 1.5667945548942017E-4 0.9547218080251552 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12861 0.5133236237829968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11046 0.44088117162794355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6990 0.278993245489709 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3793 0.15139075538518829 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3602 0.14376733480027634 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3492 0.13937688315451555 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3447 0.1375807892994316 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3371 0.13454738634417868 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2865 0.11435130877367901 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2811 0.11219599614757825 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2791 0.11139773221198537 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2538 0.10129969342673553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.982639355928726E-5 2 0.0 0.0 0.0 0.0 7.982639355928726E-5 3 0.0 0.0 0.0 0.0 7.982639355928726E-5 4 0.0 0.0 0.0 3.991319677964363E-5 7.982639355928726E-5 5 0.0 0.0 0.0 3.991319677964363E-5 7.982639355928726E-5 6 3.991319677964363E-5 0.0 0.0 3.991319677964363E-5 7.982639355928726E-5 7 1.596527871185745E-4 0.0 0.0 3.991319677964363E-5 7.982639355928726E-5 8 1.596527871185745E-4 0.0 0.0 3.991319677964363E-5 1.197395903389309E-4 9 1.596527871185745E-4 0.0 0.0 7.982639355928726E-5 1.197395903389309E-4 10-11 1.596527871185745E-4 0.0 0.0 7.982639355928726E-5 1.197395903389309E-4 12-13 1.596527871185745E-4 0.0 0.0 7.982639355928726E-5 1.9956598389821815E-4 14-15 2.394791806778618E-4 0.0 0.0 9.978299194910908E-5 2.394791806778618E-4 16-17 2.9934897584732727E-4 0.0 0.0 1.197395903389309E-4 2.793923774575054E-4 18-19 3.791753694066145E-4 0.0 0.0 1.396961887287527E-4 4.789583613557236E-4 20-21 3.991319677964363E-4 0.0 0.0 2.1952258228803999E-4 4.789583613557236E-4 22-23 3.991319677964363E-4 0.0 0.0 4.190885661862581E-4 4.789583613557236E-4 24-25 5.188715581353672E-4 0.0 0.0 5.986979516946544E-4 4.789583613557236E-4 26-27 5.188715581353672E-4 0.0 0.0 0.0011774393049994872 5.38828156525189E-4 28-29 5.188715581353672E-4 0.0 0.0 0.0038715800876254324 6.38611148474298E-4 30-31 5.188715581353672E-4 0.0 0.0 0.011794349648384692 6.38611148474298E-4 32-33 5.38828156525189E-4 0.0 0.0 0.03021428996219023 6.785243452539418E-4 34-35 5.787413533048327E-4 0.0 0.0 0.05869235586446596 6.785243452539418E-4 36-37 6.38611148474298E-4 0.0 0.0 0.10169882539453197 6.785243452539418E-4 38-39 6.38611148474298E-4 0.0 0.0 0.165200721470945 7.184375420335854E-4 40-41 6.38611148474298E-4 0.0 0.0 0.2343702914900674 7.184375420335854E-4 42-43 7.184375420335853E-4 0.0 0.0 0.3045177348302911 7.184375420335854E-4 44-45 8.381771323725162E-4 0.0 0.0 0.3897723231516099 7.383941404234071E-4 46-47 0.001057699714660556 0.0 0.0 0.4867214781293643 7.783073372030509E-4 48-49 0.0011175695098300216 0.0 0.0 0.5894181334433873 8.780903291521598E-4 50-51 0.0011175695098300216 0.0 0.0 0.7024123935265585 8.780903291521598E-4 52-53 0.0011175695098300216 0.0 0.0 0.8076634934344786 8.780903291521598E-4 54-55 0.0011175695098300216 0.0 0.0 0.9176842203575664 9.579167227114472E-4 56-57 0.0011973959033893089 0.0 0.0 1.0476016758753064 9.77873321101269E-4 58-59 0.0011973959033893089 0.0 0.0 1.1858011197248226 0.0010177865178809125 60-61 0.0011973959033893089 0.0 0.0 1.3363137847808586 0.0010377431162707344 62-63 0.0011973959033893089 0.0 0.0 1.481198689090965 0.0010377431162707344 64-65 0.0011973959033893089 0.0 0.0 1.6330085330423394 0.0010377431162707344 66-67 0.0011973959033893089 0.0 0.0 1.808905991250229 0.0010576997146605563 68-69 0.0012373091001689526 0.0 0.0 2.0012476865313316 0.0010776563130503781 70-71 0.0012572656985587742 0.0 0.0 2.1991572727631947 0.0010976129114401998 72-73 0.001297178895338418 0.0 0.0 2.4024551405603094 0.0012572656985587742 74-75 0.0013171354937282398 0.0 0.0 2.6049747010202213 0.001396961887287527 76-77 0.0013370920921180617 0.0 0.0 2.8227411026499567 0.001396961887287527 78-79 0.0013770052888977054 0.0 0.0 3.0691452229690865 0.001396961887287527 80-81 0.001516701477626458 0.0 0.0 3.3292794829804144 0.001396961887287527 82-83 0.0015566146744061017 0.0 0.0 3.599072736612415 0.0014767882808468143 84-85 0.0015965278711857454 0.0 0.0 3.8663913720440783 0.0015765712727959236 86-87 0.001636441067965389 0.0 0.0 4.134807620387182 0.001616484469575567 88-89 0.001636441067965389 0.0 0.0 4.420226890558414 0.001636441067965389 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6005 0.0 57.18043 1 GTATCAA 10485 0.0 41.956238 1 ATCAACG 13420 0.0 32.56113 3 TATCAAC 13575 0.0 32.33591 2 TCAACGC 13610 0.0 32.106567 4 CAACGCA 13790 0.0 31.68748 5 AACGCAG 14185 0.0 30.837639 6 TGGTATC 2440 0.0 29.626272 2 GTGGTAT 2930 0.0 26.781788 1 ACGCAGA 16290 0.0 26.735632 7 CGCAGAG 16400 0.0 26.527344 8 TAAGGTG 1900 0.0 24.024485 5 GCAGAGT 18010 0.0 23.760372 9 GAGTACT 11320 0.0 23.285667 12-13 GTGTAAG 1010 0.0 23.072676 1 GTAAGGT 1910 0.0 22.405033 4 CAGAGTA 17080 0.0 22.078766 10-11 GTACTTT 11895 0.0 21.980371 14-15 GGTAAGG 1945 0.0 21.757395 3 GTACATG 9915 0.0 21.440655 1 >>END_MODULE