FastQCFastQC Report
Thu 26 May 2016
SRR937918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502483
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137040.5476161076818504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110430.44128171899669244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67600.27013170519040486No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39020.1559251351557633No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37160.1484925172318853No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33920.13554537633222685No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33570.13414676543257237No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32350.1292716074394911No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28280.11300776069208063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.10925149141872294No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26560.1061345871280644No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA26010.10393677000003596No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26010.10393677000003596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55900.056.4416961
GTATCAA106650.045.5614281
TATCAAC134850.035.879322
ATCAACG135550.035.6947483
TCAACGC138000.035.0265964
TGGTATC22600.034.2788732
CAACGCA141850.034.0759245
AACGCAG145250.033.4345326
ACGCAGA162350.029.700937
GTGGTAT27050.029.4686071
CGCAGAG164350.029.2521938
GCAGAGT179200.026.2438439
GAGTACT115750.024.2945712-13
GTACATG100650.023.7119161
GTAAGGT17300.023.3522594
TACATGG98450.023.3173272
GTACTTT121750.022.7668114-15
CAGAGTA171550.022.5114810-11
ACATGGG99600.022.332773
AGAGTAC165200.021.73781810-11
Produced by FastQC (version 0.11.5)