##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2502483 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.880584603371933 33.0 31.0 34.0 30.0 34.0 2 32.08213202647131 34.0 31.0 34.0 30.0 34.0 3 32.154886167058876 34.0 31.0 34.0 30.0 34.0 4 35.680319107062864 37.0 35.0 37.0 35.0 37.0 5 35.69118991018121 37.0 35.0 37.0 35.0 37.0 6 35.7120839582127 37.0 35.0 37.0 35.0 37.0 7 35.66180909121061 37.0 35.0 37.0 33.0 37.0 8 35.62797949076977 37.0 35.0 37.0 33.0 37.0 9 37.26657443826791 39.0 37.0 39.0 34.0 39.0 10-11 37.186387879558026 39.0 37.0 39.0 34.0 39.0 12-13 37.088307492997956 39.0 37.0 39.0 33.5 39.0 14-15 38.272030619189024 40.0 38.0 41.0 33.5 41.0 16-17 38.12104098209658 40.0 38.0 41.0 33.0 41.0 18-19 38.28828747288193 40.0 38.0 41.0 33.5 41.0 20-21 38.27164540178695 40.0 38.0 41.0 34.0 41.0 22-23 38.18947621222601 40.0 38.0 41.0 33.5 41.0 24-25 38.07757715037425 40.0 38.0 41.0 33.0 41.0 26-27 37.90404690061831 40.0 38.0 41.0 33.0 41.0 28-29 37.69192957554557 40.0 38.0 41.0 32.5 41.0 30-31 37.45266221588718 40.0 37.0 41.0 32.0 41.0 32-33 37.177163441270125 40.0 36.5 41.0 31.5 41.0 34-35 36.93427567739721 40.0 36.0 41.0 31.0 41.0 36-37 36.66161568330334 39.0 36.0 41.0 30.0 41.0 38-39 36.35475645588801 39.0 35.0 41.0 30.0 41.0 40-41 36.1144968816971 39.0 35.0 40.0 30.0 41.0 42-43 35.907395574715196 39.0 35.0 40.5 28.5 41.0 44-45 35.62123938504277 39.0 35.0 40.0 27.5 41.0 46-47 35.205891508553705 38.5 35.0 40.0 25.5 41.0 48-49 34.77571955533764 38.0 34.0 40.0 24.0 41.0 50-51 34.057040147725274 37.5 33.5 39.5 23.0 40.5 52-53 34.16573619081528 38.0 34.0 40.0 23.0 40.5 54-55 34.263795398410295 38.0 34.0 40.0 23.0 41.0 56-57 33.965395169517635 38.0 34.0 40.0 21.5 41.0 58-59 33.63695018108015 38.0 33.0 40.0 19.5 41.0 60-61 33.22080469677516 37.0 33.0 40.0 18.0 41.0 62-63 32.68552393762515 36.5 32.0 39.5 13.0 41.0 64-65 32.16465046915404 36.0 31.5 39.0 8.0 40.5 66-67 31.631247245236032 35.0 31.0 39.0 6.5 40.0 68-69 31.02714783676852 35.0 30.5 38.0 2.0 40.0 70-71 30.471465140822133 35.0 29.5 37.5 2.0 40.0 72-73 29.834111560398213 34.5 29.0 37.0 2.0 39.0 74-75 29.15320323854348 34.0 28.0 36.0 2.0 39.0 76-77 28.484488406115048 34.0 26.5 36.0 2.0 38.5 78-79 27.760885088929676 33.5 26.0 35.0 2.0 37.5 80-81 27.114271305739138 33.0 25.0 35.0 2.0 37.0 82-83 26.368395509579884 32.5 23.5 35.0 2.0 36.0 84-85 25.710881952045227 32.0 20.5 35.0 2.0 36.0 86-87 25.02121133290416 31.5 18.5 34.5 2.0 35.5 88-89 24.199825533280347 31.0 11.0 34.0 2.0 35.0 90-91 23.367903997749437 30.5 4.5 34.0 2.0 35.0 92-93 22.477170074681826 30.0 2.0 34.0 2.0 35.0 94-95 21.524816552200356 29.0 2.0 34.0 2.0 35.0 96-97 20.323722079230908 28.5 2.0 34.0 2.0 35.0 98-99 18.823198998754435 26.0 2.0 33.5 2.0 35.0 100-101 15.151889343504033 10.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4248005685406717 1101 2 0.3533954817682172 1101 3 0.2234974016049307 1101 4 0.22746285716592496 1101 5 0.22455505052530356 1101 6 0.3277349381224042 1101 7 0.24431048230364638 1101 8 0.3129171789474725 1101 9 0.3572082263503802 1101 10-11 0.3555587862056413 1101 12-13 0.41955770778647405 1101 14-15 0.5447948560163951 1101 16-17 0.5265434027279738 1101 18-19 0.4658400328609176 1101 20-21 0.545675709859907 1101 22-23 0.49122135015144863 1101 24-25 0.4094049003315092 1101 26-27 0.454376612088474 1101 28-29 0.4306042650751536 1101 30-31 0.6114842027627034 1101 32-33 0.4637021303286133 1101 34-35 0.5793391339395129 1101 36-37 0.6985055072479938 1101 38-39 0.6550739220956956 1101 40-41 0.6996520010405902 1101 42-43 0.8578355622932108 1101 44-45 0.8742640513203597 1101 46-47 0.9988687722997938 1101 48-49 1.103088540720087 1101 50-51 1.1766363405989821 1101 52-53 1.150439628716299 1101 54-55 1.3549581527623076 1101 56-57 1.278224976233183 1101 58-59 1.2729610893953947 1101 60-61 1.149490085839524 1101 62-63 1.230842769303301 1101 64-65 1.287553574791609 1101 66-67 1.3030567609821588 1101 68-69 1.5745009844542999 1101 70-71 1.9075042985941408 1101 72-73 2.0739770493180636 1101 74-75 2.140744144229334 1101 76-77 2.0317310587606947 1101 78-79 2.2163747412143167 1101 80-81 2.5006888461573773 1101 82-83 2.553847295697256 1101 84-85 2.7923338514040523 1101 86-87 2.9123221105531094 1101 88-89 3.1893299880315844 1101 90-91 3.4048339815642663 1101 92-93 3.7559234376325357 1101 94-95 4.13230098583945 1101 96-97 4.42317458743463 1101 98-99 4.6342646547966275 1101 100-101 4.476042298256674 1102 1 0.3038353068043129 1102 2 0.31967985732025284 1102 3 0.2570659142798135 1102 4 0.1731650986898643 1102 5 0.25797915426805673 1102 6 0.24791483149532922 1102 7 0.1105600105130975 1102 8 0.20564759855388104 1102 9 0.3013183014797107 1102 10-11 0.24599700360479204 1102 12-13 0.33364115027918473 1102 14-15 0.49734986124349945 1102 16-17 0.39579096075942033 1102 18-19 0.3010297674709008 1102 20-21 0.35618358333219646 1102 22-23 0.45900589897488686 1102 24-25 0.40426206966546374 1102 26-27 0.37096433327498346 1102 28-29 0.4998520161666704 1102 30-31 0.3837886739373815 1102 32-33 0.42020782029152315 1102 34-35 0.48889753798913915 1102 36-37 0.5683903565694308 1102 38-39 0.5262890518272343 1102 40-41 0.7980063570066704 1102 42-43 0.8656311348466303 1102 44-45 0.9137758217101606 1102 46-47 1.0351184066922912 1102 48-49 1.1055148231457324 1102 50-51 1.0390610592380654 1102 52-53 0.9084038875055995 1102 54-55 0.9086926584850943 1102 56-57 1.044514274816997 1102 58-59 1.076571602288432 1102 60-61 1.1083473439811087 1102 62-63 0.981460899381382 1102 64-65 1.205712998005346 1102 66-67 1.1990105115118723 1102 68-69 1.4278214728576692 1102 70-71 1.6227776668263019 1102 72-73 1.769808689353706 1102 74-75 1.7553901637650853 1102 76-77 1.8505703430097924 1102 78-79 2.10598531009477 1102 80-81 2.1113139070919615 1102 82-83 2.4208056439525265 1102 84-85 2.478107158339636 1102 86-87 2.5323247747820723 1102 88-89 2.878320447968239 1102 90-91 3.058023478710357 1102 92-93 3.4226612147582003 1102 94-95 3.6754769398216496 1102 96-97 3.4936999135372204 1102 98-99 3.7176844629528354 1102 100-101 3.5033317252254275 1103 1 0.6487608470576127 1103 2 0.6145088862821666 1103 3 0.5510377137186353 1103 4 0.44372721269240856 1103 5 0.3659269831625309 1103 6 0.5276610664098982 1103 7 0.5429799910110162 1103 8 0.4835971989362875 1103 9 0.6006303848909695 1103 10-11 0.6874510112650754 1103 12-13 0.6659010008410462 1103 14-15 0.8567873453444506 1103 16-17 0.8703868358643589 1103 18-19 0.8625941372578936 1103 20-21 0.8908737626656063 1103 22-23 0.8991470073066594 1103 24-25 0.9926330484271375 1103 26-27 0.9719847237971422 1103 28-29 1.051387945601725 1103 30-31 1.0534554568322179 1103 32-33 1.0841180532275843 1103 34-35 1.1187336832134278 1103 36-37 1.1835289895330732 1103 38-39 1.2814001537578363 1103 40-41 1.260364371350562 1103 42-43 1.3436599694612852 1103 44-45 1.4792678878466035 1103 46-47 1.5078956439928675 1103 48-49 1.555836374026164 1103 50-51 1.6823620844333789 1103 52-53 1.601201273516864 1103 54-55 1.6508237382642434 1103 56-57 1.7267917392227687 1103 58-59 1.9062735724569322 1103 60-61 1.9135135326753314 1103 62-63 1.9952442654913582 1103 64-65 1.9879900582693963 1103 66-67 2.225404783655975 1103 68-69 2.388354675009584 1103 70-71 2.3754233919160725 1103 72-73 2.588813027815224 1103 74-75 2.7909683322980214 1103 76-77 2.9225297995110076 1103 78-79 3.1136265076744998 1103 80-81 3.1500611081297976 1103 82-83 3.3337356647905203 1103 84-85 3.4072810948799557 1103 86-87 3.642679267704313 1103 88-89 3.926245146484124 1103 90-91 4.043971695131848 1103 92-93 4.279954614697086 1103 94-95 4.720191742612833 1103 96-97 4.921419276618828 1103 98-99 5.251673793162002 1103 100-101 4.906712438189823 1104 1 0.19935655898084903 1104 2 0.23090901061463853 1104 3 0.23884069470630465 1104 4 0.1962545856076403 1104 5 0.2691763265137652 1104 6 0.2202103183968518 1104 7 0.2960831289714889 1104 8 0.21994618877598526 1104 9 0.2404844315254806 1104 10-11 0.263935967712861 1104 12-13 0.35462981611943434 1104 14-15 0.45149536792345657 1104 16-17 0.529015638090705 1104 18-19 0.4395036435895676 1104 20-21 0.47030239474415936 1104 22-23 0.47046052817547235 1104 24-25 0.511633294920145 1104 26-27 0.5653583442917522 1104 28-29 0.504589414396797 1104 30-31 0.5730753166692892 1104 32-33 0.5597207778051541 1104 34-35 0.5876822235465937 1104 36-37 0.6640340153882036 1104 38-39 0.6797202516665806 1104 40-41 0.6455440151671397 1104 42-43 0.7355952223256068 1104 44-45 0.8943818842030993 1104 46-47 0.9550291434169935 1104 48-49 1.1107050411997577 1104 50-51 1.0984467878723763 1104 52-53 1.0513941138272997 1104 54-55 1.2300567946361483 1104 56-57 1.277549833280652 1104 58-59 1.2717413569683558 1104 60-61 1.450809891647829 1104 62-63 1.4902927885515638 1104 64-65 1.6122238655946362 1104 66-67 1.6863599225434172 1104 68-69 1.9233499203377384 1104 70-71 1.93520665428278 1104 72-73 2.0157276463667806 1104 74-75 2.1004427612610144 1104 76-77 2.0883928538974423 1104 78-79 2.2192513223693346 1104 80-81 2.3307937558314613 1104 82-83 2.4178262520975053 1104 84-85 2.7279334592150626 1104 86-87 3.021298058585426 1104 88-89 3.233251367000495 1104 90-91 3.2861040501973378 1104 92-93 3.5047374887809575 1104 94-95 3.844343795399631 1104 96-97 4.0724653966283455 1104 98-99 4.30449951891794 1104 100-101 4.383321932002195 1105 1 0.24907637141600603 1105 2 0.19058166301419277 1105 3 0.14818805320353334 1105 4 0.09426065417615348 1105 5 0.04672799952751916 1105 6 0.10938861096411756 1105 7 0.13650703476770332 1105 8 0.08276196830446025 1105 9 0.1718852880770143 1105 10-11 0.0997759380545844 1105 12-13 0.08705287980564691 1105 14-15 0.2263626980877831 1105 16-17 0.28160026561332074 1105 18-19 0.22332519129022188 1105 20-21 0.155313790111677 1105 22-23 0.14644987457745628 1105 24-25 0.19777125466954004 1105 26-27 0.25145121333302 1105 28-29 0.29393664473326453 1105 30-31 0.2635834803537236 1105 32-33 0.45661036293000024 1105 34-35 0.31720838506603855 1105 36-37 0.4505475643254542 1105 38-39 0.48991048600385056 1105 40-41 0.493136015059612 1105 42-43 0.4677990842158195 1105 44-45 0.5051059081260263 1105 46-47 0.5234807456922539 1105 48-49 0.6026951496894029 1105 50-51 0.5737037892793353 1105 52-53 0.5067732243103151 1105 54-55 0.46688147727414275 1105 56-57 0.523329046042214 1105 58-59 0.5175930899675976 1105 60-61 0.5512603454776013 1105 62-63 0.5209007050732666 1105 64-65 0.6990405928875987 1105 66-67 0.9798359613254917 1105 68-69 1.1284062845043685 1105 70-71 1.154868578688557 1105 72-73 1.3774675006448653 1105 74-75 1.4252981818518222 1105 76-77 1.441665970426527 1105 78-79 1.5119329988496801 1105 80-81 1.6674759143213151 1105 82-83 1.8379435692113155 1105 84-85 1.9508096399742563 1105 86-87 2.086823219200557 1105 88-89 2.449365035596138 1105 90-91 2.571495879854801 1105 92-93 2.7786328289493234 1105 94-95 3.225677711395562 1105 96-97 3.433295465769511 1105 98-99 3.5880233272807125 1105 100-101 3.4464092247998384 1106 1 0.21928388365517648 1106 2 0.1256498120451539 1106 3 0.06505926689133901 1106 4 0.12355207311251348 1106 5 0.17087927214443965 1106 6 -0.050846958682001286 1106 7 0.14203439288079522 1106 8 0.25229881919396036 1106 9 0.13028464543636176 1106 10-11 0.14070558285245482 1106 12-13 0.31295125521693024 1106 14-15 0.4144300713491802 1106 16-17 0.21243487252207416 1106 18-19 0.17906181752469053 1106 20-21 0.13463277089241643 1106 22-23 0.09884420656522508 1106 24-25 0.18160138438457096 1106 26-27 0.18375094181367757 1106 28-29 0.18318406872558768 1106 30-31 0.25861102996197616 1106 32-33 0.2764257995206236 1106 34-35 0.238771689208086 1106 36-37 0.416677238979446 1106 38-39 0.22000324797596704 1106 40-41 0.5514823900499763 1106 42-43 0.48329229745578317 1106 44-45 0.33066222300036685 1106 46-47 0.570312479561693 1106 48-49 0.5314303455222742 1106 50-51 0.610588022191294 1106 52-53 0.6730356913798801 1106 54-55 0.21933298249958 1106 56-57 0.6660395958964216 1106 58-59 0.7930093319391815 1106 60-61 0.8960506062658808 1106 62-63 0.8106939208828194 1106 64-65 0.8500986189823365 1106 66-67 0.9080688647226012 1106 68-69 0.8514437037613156 1106 70-71 0.9531060889821887 1106 72-73 1.350134318631465 1106 74-75 1.3453966703247957 1106 76-77 1.5179980548606018 1106 78-79 1.78973849482216 1106 80-81 1.874207948342601 1106 82-83 2.0498884932434045 1106 84-85 2.1654189775967545 1106 86-87 1.9173100757798558 1106 88-89 2.2150531089345016 1106 90-91 2.330709519939333 1106 92-93 2.7623705760117403 1106 94-95 3.098609655359059 1106 96-97 3.2039218813348285 1106 98-99 3.4743978649085925 1106 100-101 3.413924333340278 1107 1 0.2978916620568661 1107 2 0.20498007219738668 1107 3 0.09235761143060728 1107 4 0.09114490797509234 1107 5 0.09932609072581755 1107 6 0.0968118476576123 1107 7 0.12021573800510765 1107 8 0.21872950945434866 1107 9 0.21836700394606368 1107 10-11 0.19970558873226452 1107 12-13 0.16925948371813604 1107 14-15 0.30019453494874426 1107 16-17 0.3952988254600669 1107 18-19 0.26770356653320704 1107 20-21 0.36619302278351284 1107 22-23 0.3452084990622879 1107 24-25 0.28211079649169335 1107 26-27 0.3336706823964235 1107 28-29 0.42647439614285076 1107 30-31 0.5288981411227454 1107 32-33 0.5382137176854229 1107 34-35 0.5642766179594787 1107 36-37 0.5863346959024867 1107 38-39 0.5713188556397171 1107 40-41 0.681439585027448 1107 42-43 0.687671801023356 1107 44-45 0.7305322056843266 1107 46-47 0.9619933557710993 1107 48-49 0.9123651325408346 1107 50-51 0.7662837949013337 1107 52-53 0.7801643739788702 1107 54-55 0.8391239846251324 1107 56-57 0.7923121339461474 1107 58-59 0.9399644719227709 1107 60-61 0.9260865172102584 1107 62-63 0.9777798425578581 1107 64-65 1.0241343300131476 1107 66-67 1.1432606347514422 1107 68-69 1.187167229178268 1107 70-71 1.4405256458208484 1107 72-73 1.51951542444748 1107 74-75 1.6709479760837738 1107 76-77 1.994345119512058 1107 78-79 2.0617328825427634 1107 80-81 2.136544893839698 1107 82-83 2.387075283072509 1107 84-85 2.462270853403389 1107 86-87 2.5296052746203586 1107 88-89 2.79876669453132 1107 90-91 3.0334031347588066 1107 92-93 3.2678750343782674 1107 94-95 3.650739322826361 1107 96-97 3.8353152200971863 1107 98-99 4.034372375928829 1107 100-101 3.970098355160255 1108 1 0.15360314686723697 1108 2 0.024253302884453376 1108 3 0.023138842764190315 1108 4 0.057184500107261726 1108 5 0.012191776841874002 1108 6 0.0900747200197145 1108 7 0.06466096326705184 1108 8 0.11722565494876136 1108 9 0.11573244761827794 1108 10-11 0.08359396618569548 1108 12-13 0.13480470124999044 1108 14-15 0.2568828224519919 1108 16-17 0.29698785083585477 1108 18-19 0.2584042643498137 1108 20-21 0.1264989939291823 1108 22-23 0.08420225174147333 1108 24-25 0.11308528015659647 1108 26-27 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8793.0 12 11550.0 13 14330.0 14 16712.0 15 21041.0 16 25567.0 17 32080.0 18 30878.0 19 25290.0 20 24583.0 21 25970.0 22 28171.0 23 31304.0 24 34838.0 25 39307.0 26 44279.0 27 51115.0 28 58810.0 29 68846.0 30 81128.0 31 98156.0 32 119439.0 33 150895.0 34 197955.0 35 267945.0 36 348235.0 37 385049.0 38 209562.0 39 13453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08209719183444 18.590497601316766 14.672193339890402 22.65521186695839 2 15.460223204666374 19.761572290864056 40.65084104512904 24.127363459340522 3 19.297838083993792 24.079417518504407 29.781329550556723 26.841414846945078 4 12.650319400613572 16.688916956640124 37.07198178714809 33.588781855598214 5 13.457251479155053 37.50062989568927 34.21153456987523 14.830584055280447 6 32.05902784582117 36.14649700633527 17.25307515016315 14.541399997680415 7 28.41862863859338 33.402537070575015 20.118018158484954 18.06081613234665 8 26.67909142551721 34.46787212412606 19.49475845477256 19.35827799558417 9 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26.3218430553304 30.071203649714263 22.42666100038519 34-35 22.104190009185416 26.56936188841768 29.718570433353758 21.607877669043145 36-37 22.214382118059444 25.987373390091246 29.81505438589197 21.98319010595734 38-39 21.648998497291267 26.403265948268366 29.708425741226723 22.239309813213644 40-41 22.232163698746216 26.252566086725736 29.704192554031717 21.811077660496334 42-43 21.817125882438525 26.28557633198155 29.569174946460137 22.328122839119796 44-45 21.840221110134824 26.64746014500211 29.705269750247943 21.807048994615126 46-47 22.305231311363293 26.955621170508763 28.8097254737687 21.929422044359242 48-49 22.039677879540186 26.961979728970814 28.733306499288314 22.265035892200686 50-51 22.29880996463526 27.230729294581664 28.624416158070815 21.846044582712267 52-53 22.62018643277607 26.922044705578134 28.555324836541097 21.902444025104696 54-55 21.924822716011093 27.244761214663605 28.736732427172978 22.093683642152325 56-57 22.09844525417043 27.395476005772796 28.48015312054246 22.025925619514318 58-59 22.0754023984442 26.886598655061583 28.419877208225657 22.618121738268556 60-61 21.638699082166564 27.145479866676446 28.86642005392813 22.349400997228855 62-63 21.817969992260362 27.10880198652747 29.016334376409468 22.0568936448027 64-65 21.64786400211987 27.176434899695806 28.764839396987817 22.410861701196506 66-67 21.532304281207463 27.200070496891183 28.731739346936624 22.53588587496473 68-69 22.245222373092023 26.771436250515805 28.802943060139103 22.180398316253076 70-71 21.984102368977236 26.94112089169979 28.373410476705114 22.701366262617864 72-73 21.97459382614948 27.108345133567457 28.423117028347338 22.49394401193572 74-75 22.006866136319584 27.484408315298737 28.195081400483986 22.313644147897698 76-77 22.115115432149953 27.076035252589943 28.293810215098347 22.515039100161758 78-79 22.222115646123925 27.3263380779014 28.171679706585344 22.279866569389334 80-81 22.422832659880175 27.378888342665757 27.807020699192257 22.391258298261814 82-83 22.263782875141455 27.500784840890617 27.911419989492824 22.3240122944751 84-85 22.29190196318224 27.569617794531286 27.88722312943766 22.25125711284881 86-87 22.567875461732566 27.450696742381446 27.536214798979618 22.445212996906363 88-89 22.386881429581855 27.514178481484986 27.421244148884398 22.67769594004876 90-91 22.356113612525263 27.53587965923917 27.451217094323212 22.656789633912354 92-93 22.492988757632233 27.114893249663375 27.655129607566266 22.736988385138122 94-95 22.305766364608623 26.93119380382276 27.83492956609211 22.928110265476505 96-97 22.72333739306238 26.504841096982222 27.749920157773833 23.021901352181562 98-99 22.38390852055971 26.716243743993306 27.395948391929238 23.503899343517745 100-101 23.095671821337472 25.768398381004904 27.692195640046517 23.44373415761111 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 933.0 1 648.0 2 525.5 3 703.0 4 585.5 5 550.0 6 1086.0 7 2359.5 8 3956.0 9 4593.0 10 3794.5 11 2932.5 12 2823.0 13 2966.5 14 3139.5 15 3383.5 16 3598.0 17 3861.5 18 4297.0 19 4760.5 20 5345.0 21 6385.5 22 7769.5 23 9337.0 24 11476.5 25 14486.5 26 18501.5 27 23006.5 28 26589.0 29 29727.5 30 33335.5 31 39000.5 32 47194.5 33 50472.0 34 50596.0 35 53545.0 36 57315.5 37 60615.0 38 63486.5 39 65826.0 40 68927.0 41 73551.0 42 75991.0 43 77786.0 44 80303.5 45 84229.0 46 86519.5 47 88085.0 48 89406.5 49 87724.5 50 85542.0 51 83004.0 52 81277.0 53 81965.5 54 84134.5 55 80155.0 56 73172.5 57 67640.5 58 63358.5 59 56701.5 60 45507.0 61 37501.0 62 32084.5 63 24460.0 64 17011.5 65 12609.0 66 9481.5 67 7177.0 68 5549.5 69 4407.0 70 3485.5 71 2397.0 72 1684.5 73 1359.0 74 995.0 75 716.5 76 531.0 77 357.5 78 216.0 79 131.0 80 94.0 81 62.5 82 44.0 83 29.0 84 17.5 85 11.5 86 10.0 87 10.0 88 6.5 89 5.5 90 4.5 91 3.0 92 3.0 93 1.5 94 2.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.460862271591855 2 0.04043983515572334 3 0.05282753169552001 4 0.05562475349482894 5 0.08267788432528812 6 0.08135919404847106 7 0.04687344529413387 8 0.040999279515585116 9 0.04695336591697127 10-11 0.04919114335641841 12-13 0.04485544956748957 14-15 0.03912114487890627 16-17 0.038801462387556676 18-19 0.04651380249136557 20-21 0.055884495519050476 22-23 0.047033286539808664 24-25 0.06491552589967645 26-27 0.05638399941178422 28-29 0.043976322716278195 30-31 0.04611419937717859 32-33 0.04541489392735135 34-35 0.04937096475780255 36-37 0.04391638224915015 38-39 0.02667350787198155 40-41 0.02473543276817465 42-43 0.024335829653987662 44-45 0.01524485880623365 46-47 0.027252932387552685 48-49 0.027412773633227475 50-51 0.016563549083050716 52-53 0.01604406503460763 54-55 0.016943172041528357 56-57 0.016064045190316976 58-59 0.01442567242215032 60-61 0.01796215998270518 62-63 0.017122993442912498 64-65 0.017742378269902333 66-67 0.009790276297581243 68-69 0.013007081366786508 70-71 0.018981147923882 72-73 0.016543568927341365 74-75 0.013906188373707235 76-77 0.010209859567477582 78-79 0.014325771643603573 80-81 0.018102021072670622 82-83 0.015324779429071048 84-85 0.012827259965402361 86-87 0.003496527249136158 88-89 0.028631563131497798 90-91 0.03144876508651607 92-93 0.017182933910040547 94-95 0.0324277927162742 96-97 0.026933249896203094 98-99 0.023476682958485633 100-101 0.14581517636683244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2502483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.635387144050064 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2368057169924 39.60118396395574 2 13.83121911258984 14.560231453307015 3 4.800186792328522 7.579790705339027 4 2.112055299648576 4.446753934665818 5 1.0970342082902365 2.887141013181153 6 0.6523916300287309 2.0603331609660076 7 0.4428879830314394 1.6318106313815102 8 0.3091765059837948 1.3018900070641402 9 0.21058368224611831 0.9975738277119653 >10 1.1601203097590564 11.205254473110196 >50 0.092732746415864 3.3481681996168593 >100 0.04802999123495198 4.716371897603888 >500 0.0032738081165488344 1.2290292039449873 >1k 0.0032738081165470533 3.171856320460624 >5k 7.613507247783846E-5 0.27090017342152645 >10k+ 1.5227014495567691E-4 0.9917110342696027 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13704 0.5476161076818504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11043 0.44128171899669244 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6760 0.27013170519040486 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3902 0.1559251351557633 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3716 0.1484925172318853 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3392 0.13554537633222685 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3357 0.13414676543257237 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3235 0.1292716074394911 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2828 0.11300776069208063 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2734 0.10925149141872294 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2656 0.1061345871280644 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2601 0.10393677000003596 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2601 0.10393677000003596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.996031141869895E-5 0.0 0.0 0.0 3.996031141869895E-5 6 3.996031141869895E-5 0.0 0.0 0.0 7.99206228373979E-5 7 3.996031141869895E-5 0.0 0.0 0.0 7.99206228373979E-5 8 3.996031141869895E-5 0.0 0.0 0.0 7.99206228373979E-5 9 3.996031141869895E-5 0.0 0.0 3.996031141869895E-5 7.99206228373979E-5 10-11 3.996031141869895E-5 0.0 0.0 3.996031141869895E-5 1.598412456747958E-4 12-13 9.990077854674738E-5 0.0 0.0 3.996031141869895E-5 2.7972217993089266E-4 14-15 1.1988093425609684E-4 0.0 0.0 3.996031141869895E-5 3.196824913495916E-4 16-17 1.1988093425609684E-4 0.0 0.0 3.996031141869895E-5 3.5964280276829056E-4 18-19 1.5984124567479578E-4 0.0 0.0 3.996031141869895E-5 3.9960311418698946E-4 20-21 1.9980155709349473E-4 0.0 0.0 9.990077854674738E-5 3.9960311418698946E-4 22-23 1.9980155709349473E-4 0.0 0.0 3.196824913495916E-4 4.395634256056884E-4 24-25 1.9980155709349473E-4 0.0 0.0 4.1958326989633896E-4 4.395634256056884E-4 26-27 1.9980155709349473E-4 0.0 0.0 0.0010189879411768232 4.5954358131503786E-4 28-29 3.3966264705894106E-4 0.0 0.0 0.003296725692042663 4.7952373702438736E-4 30-31 3.9960311418698946E-4 0.0 0.0 0.010969105484432862 4.7952373702438736E-4 32-33 3.9960311418698946E-4 0.0 0.0 0.027392793477518128 5.194840484430863E-4 34-35 3.9960311418698946E-4 0.0 0.0 0.05420616243946512 5.994046712804842E-4 36-37 3.9960311418698946E-4 0.0 0.0 0.09666399332183276 5.994046712804842E-4 38-39 3.9960311418698946E-4 0.0 0.0 0.16135973750870636 6.393649826991832E-4 40-41 3.9960311418698946E-4 0.0 0.0 0.2286928622492141 6.393649826991832E-4 42-43 5.194840484430863E-4 0.0 0.0 0.29638562979249006 6.993054498272316E-4 44-45 6.793252941178821E-4 0.0 0.0 0.3804021845503046 7.5924591695528E-4 46-47 7.5924591695528E-4 0.0 0.0 0.4781051459690236 8.191863840833285E-4 48-49 8.391665397926779E-4 0.0 0.0 0.5833606062458766 8.391665397926779E-4 50-51 8.391665397926779E-4 0.0 0.0 0.6975272159690995 8.791268512113768E-4 52-53 8.391665397926779E-4 0.0 0.0 0.8055399377338428 8.791268512113768E-4 54-55 8.391665397926779E-4 0.0 0.0 0.91864759920447 8.791268512113768E-4 56-57 8.391665397926779E-4 0.0 0.0 1.0513158331145507 8.791268512113768E-4 58-59 8.391665397926779E-4 0.0 0.0 1.188839244862003 8.991070069207263E-4 60-61 8.391665397926779E-4 0.0 0.0 1.338110988166553 9.190871626300758E-4 62-63 8.391665397926779E-4 0.0 0.0 1.4824476330108936 9.990077854674737E-4 64-65 8.391665397926779E-4 0.0 0.0 1.628422650623401 9.990077854674737E-4 66-67 8.791268512113768E-4 0.0 0.0 1.801151096730727 9.990077854674737E-4 68-69 9.190871626300758E-4 0.0 0.0 1.9897837467826953 9.990077854674737E-4 70-71 9.790276297581243E-4 0.0 0.0 2.185928935381379 9.990077854674737E-4 72-73 0.001058948252595522 0.0 0.0 2.3902260275094775 0.0010389680968861726 74-75 0.0011588490311422695 0.0 0.0 2.58980780289017 0.0011188887197235706 76-77 0.0011588490311422695 0.0 0.0 2.8034156475788246 0.0011188887197235706 78-79 0.0011588490311422695 0.0 0.0 3.0485721581325427 0.0011588490311422695 80-81 0.0012387696539796673 0.0 0.0 3.3027397189111776 0.0011988093425609684 82-83 0.0012787299653983664 0.0 0.0 3.564279957146562 0.0011988093425609684 84-85 0.0012987101211077159 0.0 0.0 3.8290769607625705 0.0011988093425609684 86-87 0.0013386704325264148 0.0 0.0 4.102065828219413 0.0012987101211077157 88-89 0.0013586505882357642 0.0 0.0 4.388940903894252 0.0013586505882357642 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5590 0.0 56.441696 1 GTATCAA 10665 0.0 45.561428 1 TATCAAC 13485 0.0 35.87932 2 ATCAACG 13555 0.0 35.694748 3 TCAACGC 13800 0.0 35.026596 4 TGGTATC 2260 0.0 34.278873 2 CAACGCA 14185 0.0 34.075924 5 AACGCAG 14525 0.0 33.434532 6 ACGCAGA 16235 0.0 29.70093 7 GTGGTAT 2705 0.0 29.468607 1 CGCAGAG 16435 0.0 29.252193 8 GCAGAGT 17920 0.0 26.243843 9 GAGTACT 11575 0.0 24.29457 12-13 GTACATG 10065 0.0 23.711916 1 GTAAGGT 1730 0.0 23.352259 4 TACATGG 9845 0.0 23.317327 2 GTACTTT 12175 0.0 22.76681 14-15 CAGAGTA 17155 0.0 22.51148 10-11 ACATGGG 9960 0.0 22.33277 3 AGAGTAC 16520 0.0 21.737818 10-11 >>END_MODULE