FastQCFastQC Report
Thu 26 May 2016
SRR937918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502483
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131060.5237198414534685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109510.43760537034617214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.27924265619386823No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38350.15324779429071045No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36310.14509589076129586No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34810.13910184404849105No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34720.13874220124572276No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34350.13726366972323087No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28120.11236839570938145No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27580.1102105388927717No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA26440.10565506339104001No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25580.10221847660903192No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25170.10058010384086526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54450.060.5474241
GTATCAA102350.043.1183321
TATCAAC133600.032.854862
ATCAACG132850.032.8218573
TCAACGC135400.032.3778274
CAACGCA136750.032.023465
AACGCAG141150.031.0925086
TGGTATC21450.028.3476282
ACGCAGA162000.026.973517
CGCAGAG162050.026.847958
TAAGGTG18850.024.441345
GCAGAGT177950.024.1020749
TAAGGTA9750.023.8702034
GTACATG95750.023.466941
GTGGTAT27000.023.4003281
TACATGG93450.023.3836672
GTAAGGT18900.023.3714564
GTGTAAG11300.023.1216261
GAGTACT116800.022.65045712-13
ACATGGG94450.022.2783873
Produced by FastQC (version 0.11.5)