##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937918_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2502483 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49443053159602 34.0 31.0 34.0 31.0 34.0 2 32.73243414640579 34.0 31.0 34.0 31.0 34.0 3 32.68698808343553 34.0 31.0 34.0 31.0 34.0 4 36.21142161605094 37.0 35.0 37.0 35.0 37.0 5 36.1793722474838 37.0 35.0 37.0 35.0 37.0 6 36.239686343523616 37.0 37.0 37.0 35.0 37.0 7 36.20019117012983 37.0 37.0 37.0 35.0 37.0 8 36.19351060526685 37.0 37.0 37.0 35.0 37.0 9 37.89994697266675 39.0 38.0 39.0 35.0 39.0 10-11 37.867961540597875 39.0 38.0 39.0 35.0 39.0 12-13 37.81061329887156 39.0 38.0 39.0 35.0 39.0 14-15 39.08523474485141 40.0 38.5 41.0 36.0 41.0 16-17 39.0232786796154 40.0 38.0 41.0 36.0 41.0 18-19 39.032844378962814 40.0 38.5 41.0 36.0 41.0 20-21 39.039043621874754 40.0 38.5 41.0 35.5 41.0 22-23 39.06645000185816 40.0 39.0 41.0 35.5 41.0 24-25 39.01068638628115 40.0 38.5 41.0 35.0 41.0 26-27 38.840848269498736 40.0 38.0 41.0 35.0 41.0 28-29 38.761332644417564 40.0 38.0 41.0 35.0 41.0 30-31 38.52594003635589 40.0 38.0 41.0 34.0 41.0 32-33 38.30424342542986 40.0 38.0 41.0 34.0 41.0 34-35 38.09128693381733 40.0 38.0 41.0 34.0 41.0 36-37 37.990839897813494 40.0 37.5 41.0 33.0 41.0 38-39 37.920862998869524 40.0 38.0 41.0 33.0 41.0 40-41 37.78165386138487 40.0 37.0 41.0 33.0 41.0 42-43 37.574089414393626 40.0 37.0 41.0 33.0 41.0 44-45 37.30432314625115 40.0 37.0 41.0 32.5 41.0 46-47 36.923128948328525 40.0 36.5 41.0 31.0 41.0 48-49 36.51349339835675 40.0 36.0 41.0 30.5 41.0 50-51 36.18833374692255 39.0 35.5 41.0 30.0 41.0 52-53 35.71657289979592 39.0 35.0 40.0 28.0 41.0 54-55 35.39907943430585 38.5 35.0 40.0 27.5 41.0 56-57 35.126073583716654 38.0 35.0 40.0 27.0 41.0 58-59 34.73843818319645 38.0 34.0 40.0 26.0 41.0 60-61 34.329849593383855 37.5 34.0 40.0 26.0 41.0 62-63 33.90316697456087 37.0 33.5 40.0 24.5 41.0 64-65 33.46651965268096 36.0 33.0 39.0 23.0 41.0 66-67 32.961602136757776 36.0 32.5 39.0 22.0 40.0 68-69 32.434796560056554 35.0 32.0 38.5 20.5 40.0 70-71 31.84990127805064 35.0 31.0 38.0 18.5 40.0 72-73 31.384946071561725 35.0 31.0 37.0 17.5 39.0 74-75 30.84175436956015 34.5 30.0 36.5 12.5 39.0 76-77 29.642476092744687 33.5 28.5 35.0 12.0 37.5 78-79 30.325804411058936 34.0 30.0 36.0 9.5 38.0 80-81 30.14433384762254 34.0 30.0 35.0 7.0 37.0 82-83 29.683418428816502 34.0 29.5 35.0 4.0 37.0 84-85 29.174090493322034 34.0 29.0 35.0 2.0 36.0 86-87 28.632730571995893 34.0 29.0 35.0 2.0 36.0 88-89 28.00214526931851 33.0 27.0 35.0 2.0 35.5 90-91 27.46944035184255 33.0 26.5 35.0 2.0 35.0 92-93 26.843500834970705 33.0 25.5 35.0 2.0 35.0 94-95 26.10375435117841 32.0 24.5 34.5 2.0 35.0 96-97 25.289771798649582 32.0 21.5 34.0 2.0 35.0 98-99 24.265503102318778 31.5 9.5 34.0 2.0 35.0 100-101 20.789779191307193 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04248237569623825 1101 2 0.01516656152298168 1101 3 0.11599528999214215 1101 4 0.059825953604367044 1101 5 0.008751402341928838 1101 6 0.09371872315161056 1101 7 0.08605732648427278 1101 8 0.06060947586637866 1101 9 0.09074068670341262 1101 10-11 0.13537181257080988 1101 12-13 0.11668313621473914 1101 14-15 0.2519211363566001 1101 16-17 0.2578954322179712 1101 18-19 0.2773517020862357 1101 20-21 0.14234058961437768 1101 22-23 0.08178193196010142 1101 24-25 0.1721724602497403 1101 26-27 0.18233922613125486 1101 28-29 0.12085004075234451 1101 30-31 0.23022847992506712 1101 32-33 0.2598899165952915 1101 34-35 0.2935035072395422 1101 36-37 0.29025925753128234 1101 38-39 0.31105894805280343 1101 40-41 0.3259531969217804 1101 42-43 0.45246612654358387 1101 44-45 0.4596236242705487 1101 46-47 0.322946740866108 1101 48-49 0.409875264353893 1101 50-51 0.4787756083763739 1101 52-53 0.4081714918591004 1101 54-55 0.5588817643444131 1101 56-57 0.60805193521729 1101 58-59 0.5295643565883026 1101 60-61 0.6293706298623718 1101 62-63 0.6361461247709528 1101 64-65 0.4608735874505996 1101 66-67 0.46645917971230233 1101 68-69 0.6199089558501711 1101 70-71 0.6348390792042444 1101 72-73 0.7378419945832633 1101 74-75 0.6571328815782813 1101 76-77 0.6602658456376389 1101 78-79 0.8801711958208642 1101 80-81 1.0294526297650997 1101 82-83 1.0741056891095333 1101 84-85 1.175532275877842 1101 86-87 1.2790655616518123 1101 88-89 1.431832042574321 1101 90-91 1.5284094311395862 1101 92-93 1.8963927176528657 1101 94-95 1.8745109933926223 1101 96-97 2.1813610922462416 1101 98-99 2.404088528736146 1101 100-101 2.9414620393595072 1102 1 0.0332012059066642 1102 2 0.08436373087624105 1102 3 0.23681575667625054 1102 4 0.1451812854891088 1102 5 0.03260262252983637 1102 6 0.021261348401743874 1102 7 0.07021674776740383 1102 8 0.11884585282388826 1102 9 0.11890062152633618 1102 10-11 0.07676727006042938 1102 12-13 0.13529596100210028 1102 14-15 0.13513253616760323 1102 16-17 0.21862575915555027 1102 18-19 0.25497710184124855 1102 20-21 0.27567714061554227 1102 22-23 0.28023552080200176 1102 24-25 0.1745333918418055 1102 26-27 0.23307686039246533 1102 28-29 0.18370335274610738 1102 30-31 0.11423017113127543 1102 32-33 0.1441374698002349 1102 34-35 -0.01399388403626034 1102 36-37 0.1736175760552996 1102 38-39 0.2808677317067918 1102 40-41 0.3041637617479793 1102 42-43 0.2708064470426095 1102 44-45 0.3235302720044544 1102 46-47 0.2858117329940839 1102 48-49 0.42044906783998215 1102 50-51 0.420449748076571 1102 52-53 0.3668820243477242 1102 54-55 0.3230122652291101 1102 56-57 0.4860451144050302 1102 58-59 0.5279253731267914 1102 60-61 0.6765709209969586 1102 62-63 0.557614597578926 1102 64-65 0.6747086581636736 1102 66-67 0.6836647896518144 1102 68-69 0.7625760034887108 1102 70-71 0.8533173411230486 1102 72-73 0.7879624158017293 1102 74-75 0.8889124031175122 1102 76-77 1.013413448748608 1102 78-79 0.9592757252996904 1102 80-81 1.0722051064844713 1102 82-83 1.0382767326088604 1102 84-85 1.0449166169686528 1102 86-87 1.1565044653795127 1102 88-89 1.3540499745727708 1102 90-91 1.4983961196477793 1102 92-93 1.6303903063325969 1102 94-95 1.5340466646190478 1102 96-97 1.646002912725475 1102 98-99 2.0345681768262516 1102 100-101 2.3175298304723704 1103 1 0.31759664242800767 1103 2 0.345320742993529 1103 3 0.4091289943612111 1103 4 0.24230417803028814 1103 5 0.165945146345571 1103 6 0.17603079340063488 1103 7 0.22694874993270986 1103 8 0.22641670728953045 1103 9 0.2528769742953685 1103 10-11 0.3136205955743634 1103 12-13 0.2404809198346527 1103 14-15 0.4101671520297572 1103 16-17 0.4335378931294329 1103 18-19 0.4173925716683584 1103 20-21 0.3884222441577805 1103 22-23 0.3851914659851161 1103 24-25 0.3570777020538145 1103 26-27 0.3969931554545525 1103 28-29 0.3711606793081188 1103 30-31 0.41583721828541087 1103 32-33 0.4534008698491334 1103 34-35 0.4870691209865541 1103 36-37 0.5549587563242895 1103 38-39 0.5651216063595399 1103 40-41 0.6107699298938414 1103 42-43 0.6600969829637293 1103 44-45 0.6520354213314619 1103 46-47 0.6059659669512811 1103 48-49 0.7585329375779182 1103 50-51 0.7646242582736207 1103 52-53 0.8404640650075308 1103 54-55 0.8219941648434457 1103 56-57 0.7728999779027887 1103 58-59 0.9105739519062581 1103 60-61 0.9670947178726337 1103 62-63 0.8557029173664077 1103 64-65 0.9885189895082576 1103 66-67 0.9431392751737704 1103 68-69 1.0502957302978828 1103 70-71 1.0306139831767318 1103 72-73 0.9799401799235028 1103 74-75 0.9460811702360026 1103 76-77 0.781193495956856 1103 78-79 1.2369066150199615 1103 80-81 1.4138926856148615 1103 82-83 1.3622822127707792 1103 84-85 1.51632695711454 1103 86-87 1.6498042444711523 1103 88-89 1.8133157433412812 1103 90-91 1.7221055210846856 1103 92-93 2.0479237260474825 1103 94-95 1.9633971886449366 1103 96-97 2.139388122889283 1103 98-99 2.50561467714968 1103 100-101 2.6445544739127165 1104 1 0.1702276325615273 1104 2 0.10378136445505959 1104 3 0.20854167303534155 1104 4 0.11724534652189078 1104 5 0.06268936917852841 1104 6 0.07463078278689039 1104 7 0.09548765699951645 1104 8 0.00774483128104464 1104 9 0.0808180938161982 1104 10-11 0.13285526654718183 1104 12-13 0.104588792386906 1104 14-15 0.20316681210299237 1104 16-17 0.17138807250790222 1104 18-19 0.18351978132039193 1104 20-21 0.250630911655108 1104 22-23 0.1339807714260104 1104 24-25 0.09072479660201083 1104 26-27 0.19338429703271487 1104 28-29 0.14376094284868657 1104 30-31 0.18452586524370673 1104 32-33 0.2200707534237054 1104 34-35 0.17310095853967056 1104 36-37 0.1888952291548307 1104 38-39 0.1647822328205919 1104 40-41 0.16460099059443678 1104 42-43 0.23725964150657575 1104 44-45 0.2985107659862578 1104 46-47 0.35647713771520984 1104 48-49 0.36961061606866963 1104 50-51 0.34434384635369497 1104 52-53 0.3574915478508558 1104 54-55 0.4744221209615418 1104 56-57 0.539447844365462 1104 58-59 0.47016481449449543 1104 60-61 0.4671196130516293 1104 62-63 0.47682595425860086 1104 64-65 0.5276569770311639 1104 66-67 0.34830431791664296 1104 68-69 0.5716658339130518 1104 70-71 0.6384741390676787 1104 72-73 0.6716839755428978 1104 74-75 0.6729695822575685 1104 76-77 0.6871986229294365 1104 78-79 0.8753583356866272 1104 80-81 0.9830636843500251 1104 82-83 1.1290901979967707 1104 84-85 1.2925997205798119 1104 86-87 1.2895752047263578 1104 88-89 1.275671671457122 1104 90-91 1.1973139183207664 1104 92-93 1.3887780671212688 1104 94-95 1.4252497833680202 1104 96-97 1.8181826348034171 1104 98-99 2.1757600096724303 1104 100-101 2.5671772865434015 1105 1 0.07578471458472791 1105 2 0.09579741768491346 1105 3 0.11537301690724178 1105 4 0.0796600141150634 1105 5 0.0991812714500071 1105 6 0.10841724257082319 1105 7 0.09682193559663688 1105 8 0.07880559917659014 1105 9 0.11113264722126104 1105 10-11 0.07038361430173268 1105 12-13 0.07966504022731868 1105 14-15 0.10034757223386492 1105 16-17 0.16552721821325633 1105 18-19 0.19102382854357103 1105 20-21 0.09213316525274706 1105 22-23 0.16228046568443943 1105 24-25 0.0816496078071367 1105 26-27 0.09092945572856337 1105 28-29 0.03724371788560177 1105 30-31 0.09602491794809964 1105 32-33 0.10802667362268181 1105 34-35 0.04100010977155222 1105 36-37 0.21341926039230685 1105 38-39 0.20715007172064048 1105 40-41 0.2598921140235646 1105 42-43 0.17944202240224882 1105 44-45 0.24277575931095896 1105 46-47 0.21845135427000884 1105 48-49 0.2508344232123463 1105 50-51 0.414491937680431 1105 52-53 0.18990384290695062 1105 54-55 0.3664491772619414 1105 56-57 0.3199430731902595 1105 58-59 0.39982041838646154 1105 60-61 0.3033925167088114 1105 62-63 0.3313753644533861 1105 64-65 0.46830510164843275 1105 66-67 0.4731647086444326 1105 68-69 0.4688110372463541 1105 70-71 0.6688101828449042 1105 72-73 0.5623037641276483 1105 74-75 0.498714313521635 1105 76-77 0.5825961510521296 1105 78-79 0.781844895842319 1105 80-81 0.9131269019916957 1105 82-83 0.774685046809271 1105 84-85 0.9634623238000195 1105 86-87 1.0557875414815818 1105 88-89 1.2689824609993288 1105 90-91 1.2988943749919564 1105 92-93 1.4142061787786808 1105 94-95 1.4780443601045867 1105 96-97 1.620127990179153 1105 98-99 1.7555297704212904 1105 100-101 2.1745748352968484 1106 1 -0.013117069807620396 1106 2 0.07390211720880302 1106 3 0.13185567282896926 1106 4 0.06872582360123403 1106 5 0.03169299058716035 1106 6 0.07391048640106845 1106 7 0.013163325594597097 1106 8 0.003157054601039988 1106 9 -0.010110713157963858 1106 10-11 0.02023222299450822 1106 12-13 -0.009090675566930884 1106 14-15 0.05685797965971773 1106 16-17 0.051685023690872356 1106 18-19 -0.04316543108593862 1106 20-21 0.002620492922389417 1106 22-23 0.09792447678014327 1106 24-25 0.03347281922392398 1106 26-27 0.1309160021223974 1106 28-29 0.011884220887729668 1106 30-31 0.15492528295134633 1106 32-33 0.20378175191545012 1106 34-35 -0.011215054932641522 1106 36-37 0.12052360873975232 1106 38-39 0.1437238744475806 1106 40-41 0.18952559349687448 1106 42-43 0.27239212475733865 1106 44-45 0.27674076158076133 1106 46-47 -0.14383211241655403 1106 48-49 0.1534582129078288 1106 50-51 0.23831592486677522 1106 52-53 0.12167604196892512 1106 54-55 0.07845482300444928 1106 56-57 0.31162572837781966 1106 58-59 0.23715781574077255 1106 60-61 0.33647131849969014 1106 62-63 0.24956394864945963 1106 64-65 0.25420463337239596 1106 66-67 0.29335457029070255 1106 68-69 0.0024569828715144126 1106 70-71 0.17648008433195272 1106 72-73 0.19135820014684768 1106 74-75 0.21962387274014006 1106 76-77 0.37418195740585247 1106 78-79 0.5141357225508187 1106 80-81 0.6005541735954694 1106 82-83 0.5318991530897001 1106 84-85 0.7009325441601781 1106 86-87 0.9659616246602276 1106 88-89 0.9010570755923588 1106 90-91 0.8808203514031803 1106 92-93 0.7472555833082417 1106 94-95 1.0545843055070527 1106 96-97 1.1451652185527372 1106 98-99 1.3595486605324325 1106 100-101 1.7635653315079374 1107 1 0.05286719581871324 1107 2 0.03117970601599751 1107 3 0.1703890128260852 1107 4 0.05004889862468076 1107 5 0.021881644316287918 1107 6 0.01166372250192893 1107 7 -0.007708679744276026 1107 8 0.08469709464255004 1107 9 0.09076332071562376 1107 10-11 0.034915359991160244 1107 12-13 0.07336624746928777 1107 14-15 0.09209818462991848 1107 16-17 0.11519175754417432 1107 18-19 0.1041872576045364 1107 20-21 0.10622466993997648 1107 22-23 0.11111561178942253 1107 24-25 0.11181593264252854 1107 26-27 0.14144711073132754 1107 28-29 0.1316150284503408 1107 30-31 0.14688375972756518 1107 32-33 0.10939469279101388 1107 34-35 0.0722019677075707 1107 36-37 0.1571679125260559 1107 38-39 0.21550401656261187 1107 40-41 0.15523909383643542 1107 42-43 0.11632254307647116 1107 44-45 -0.029947964176045616 1107 46-47 0.09116481538230659 1107 48-49 0.1688934512018605 1107 50-51 0.2178335304902177 1107 52-53 0.1358126505528574 1107 54-55 0.23735891447078217 1107 56-57 0.2630760572062343 1107 58-59 0.4037091891175706 1107 60-61 0.43613484632737 1107 62-63 0.39292983163534956 1107 64-65 0.5460286094768421 1107 66-67 0.7473602648874476 1107 68-69 0.8229553262348261 1107 70-71 0.8313228434919644 1107 72-73 0.7023426421675083 1107 74-75 0.8063006624810463 1107 76-77 0.7332986775298025 1107 78-79 0.8790478781776514 1107 80-81 0.7991884405447571 1107 82-83 1.0200175254404762 1107 84-85 1.2070434315118241 1107 86-87 1.3771760407803164 1107 88-89 1.4995203135453643 1107 90-91 1.5400412176757001 1107 92-93 1.7454615795392847 1107 94-95 1.589659434789393 1107 96-97 1.4952604714875015 1107 98-99 1.7109080727865447 1107 100-101 2.23083830466366 1108 1 0.05753195572904701 1108 2 0.0765089728517907 1108 3 0.12043152833898318 1108 4 0.052148673046502836 1108 5 -0.02510397068290615 1108 6 0.0020826232826678392 1108 7 0.022755617765930936 1108 8 0.005471368892202122 1108 9 -0.029437521161803204 1108 10-11 -0.028518839346858726 1108 12-13 -0.013115576270529061 1108 14-15 0.1095102196823845 1108 16-17 0.0751590100582149 1108 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-3.304208235927078 2316 100-101 -3.806402599496529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 13.0 5 45.0 6 104.0 7 205.0 8 566.0 9 1082.0 10 2032.0 11 3389.0 12 5096.0 13 7580.0 14 10484.0 15 14181.0 16 18669.0 17 26417.0 18 26324.0 19 19452.0 20 16832.0 21 16422.0 22 17301.0 23 18451.0 24 21206.0 25 24208.0 26 28637.0 27 33836.0 28 40987.0 29 49939.0 30 60903.0 31 74793.0 32 93679.0 33 123177.0 34 170995.0 35 254800.0 36 389237.0 37 539988.0 38 364034.0 39 27401.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.131002288527036 19.064185451010058 15.168414730489676 22.63639752997323 2 15.674267835183636 20.119990264578853 40.07992157310978 24.125820327127734 3 19.60283526958999 23.939381466205393 29.96698686068263 26.490796403521987 4 12.875811743776081 17.04495095471178 36.786183962088856 33.29305333942328 5 13.384709137953427 37.54846701765253 34.34575769987712 14.721066144516929 6 32.12716984751127 36.35289792525814 17.009206866788144 14.510725360442441 7 28.4858278757538 33.373133803506356 20.052603753951576 18.088434566788266 8 26.69336814675664 34.80854814997744 19.3393121951278 19.158771508138116 9 26.258360196652685 16.77581825730684 20.183513734159234 36.78230781188124 10-11 25.015434670285476 26.827255170164992 26.755406530234172 21.401903629315363 12-13 26.109388155683778 24.065118524281683 27.425860635217102 22.39963268481744 14-15 22.50562741085554 25.474498727863487 26.204273915147475 25.8155999461335 16-17 21.73441772320086 28.761839493383068 27.150959097616067 22.352783685800002 18-19 21.762165816910645 26.678782633088815 30.011652426809693 21.547399123190846 20-21 22.239571657429842 25.802373083053908 30.518489036688763 21.439566222827487 22-23 21.969819575197914 25.858517320597187 30.454152935304656 21.717510168900247 24-25 21.793010022218315 25.959403622064865 30.59979460046994 21.647791755246878 26-27 21.326453428245117 26.440618352120175 30.23948104945198 21.993447170182726 28-29 21.45965494160781 26.88164716930239 30.063177454320222 21.595520434769576 30-31 21.54596916181615 26.38716282474759 29.973142663963216 22.093725349473043 32-33 21.184375026849317 26.358165778616176 30.038185471777695 22.41927372275681 34-35 22.103959624412393 26.583585987164415 29.738268482630232 21.574185905792962 36-37 22.230412451884344 26.02813247311278 29.794601295446117 21.94685377955676 38-39 21.61438520951252 26.43285645226303 29.744405095710945 22.2083532425135 40-41 22.206858539720503 26.277912429274657 29.746352019159595 21.768877011845248 42-43 21.813948887452277 26.22209388186181 29.628311439422628 22.335645791263286 44-45 21.811437358927304 26.645954690487304 29.703635439379504 21.83897251120589 46-47 22.28942564154001 26.965630083172975 28.818584934556757 21.92635934073026 48-49 22.017011964959476 27.006234630209946 28.726652105076816 22.250101299753762 50-51 22.245905366789703 27.239265961047487 28.671843125407847 21.84298554675496 52-53 22.63330060583828 26.895427461445294 28.580034309923384 21.891237622793042 54-55 21.973475943692726 27.249975324507698 28.669665288435525 22.10688344336405 56-57 22.0997305476201 27.367158937743035 28.468245338729574 22.06486517590729 58-59 22.090559656149512 26.9373258479678 28.39084221551155 22.581272280371135 60-61 21.597269591841382 27.242922329542296 28.768846781376737 22.39096129723958 62-63 21.862266397014483 27.158925754940196 28.9093871966363 22.069420651409022 64-65 21.58574104199709 27.256289053711853 28.682192846065284 22.47577705822577 66-67 21.477842571306294 27.303226120978437 28.658062665061323 22.560868642653944 68-69 22.17397897805797 26.892077680525382 28.693810001716297 22.24013333970035 70-71 21.912096905353604 27.105438878106263 28.23355842976755 22.74890578677258 72-73 21.910798195232495 27.278706788417743 28.25369842672258 22.556796589627183 74-75 21.961228108242896 27.673374804144522 27.973916306324558 22.391480781288024 76-77 21.977451994678884 27.23854667544195 28.283928402310828 22.50007292756834 78-79 22.023885876547414 27.593194439282904 28.10256852893706 22.280351155232623 80-81 22.201935679805633 27.53928103321505 27.87372926057351 22.385054026405808 82-83 22.10116361453785 27.715436923892124 27.95875739921621 22.22464206235382 84-85 22.064806328498566 27.838875924639378 27.89482233916355 22.201495407698506 86-87 22.250343361171197 27.819307906957413 27.54835528300673 22.38199344886466 88-89 22.082516477640997 27.90389701104743 27.394482757518336 22.619103753793233 90-91 21.94071640554021 27.925361918209973 27.548622909528092 22.585298766721724 92-93 22.030883985451126 27.543814851134602 27.727354101680664 22.697947061733608 94-95 21.81784796767688 27.39109707526251 27.91429785081285 22.876757106247762 96-97 22.161269207991772 26.980080512325006 27.919111247509466 22.939539032173762 98-99 21.781467634454835 27.316865376531084 27.54186253934104 23.359804449673042 100-101 21.968077326685904 26.9780236906485 27.8733562924239 23.180542690241694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 946.0 1 652.0 2 554.5 3 752.5 4 597.0 5 557.0 6 1124.5 7 2487.5 8 4077.5 9 4761.5 10 3925.0 11 2883.0 12 2783.0 13 2950.5 14 3149.5 15 3384.5 16 3604.0 17 3871.0 18 4155.5 19 4571.0 20 5249.5 21 6126.5 22 7460.5 23 9209.5 24 11373.5 25 14296.5 26 18280.0 27 22852.0 28 26456.0 29 29807.5 30 33519.5 31 39129.0 32 47810.0 33 51272.0 34 50935.0 35 53181.5 36 56926.5 37 60801.5 38 63540.0 39 66344.5 40 70017.0 41 74630.5 42 77105.5 43 78719.5 44 81413.5 45 85699.5 46 87545.5 47 89138.0 48 90560.5 49 88182.5 50 86425.5 51 83851.5 52 81373.5 53 82525.5 54 85273.0 55 80653.5 56 72679.5 57 66617.0 58 62390.0 59 55857.5 60 44517.0 61 36706.0 62 31260.0 63 22959.5 64 15316.0 65 11157.5 66 8253.0 67 6383.0 68 5056.0 69 4048.5 70 3084.0 71 2027.0 72 1441.5 73 1189.5 74 858.0 75 596.0 76 426.0 77 262.0 78 165.0 79 90.5 80 49.0 81 32.5 82 23.5 83 14.0 84 10.0 85 6.5 86 4.5 87 2.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011188887197235705 3 0.005834205467130047 4 0.0 5 3.196824913495916E-4 6 1.1988093425609684E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.9980155709349476E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017182933910040547 26-27 0.020399738979245814 28-29 3.196824913495916E-4 30-31 3.996031141869895E-5 32-33 0.0036563684948109536 34-35 0.015065037404849503 36-37 0.007152895743947112 38-39 0.019840294619384028 40-41 0.02201813159170312 42-43 0.018201921851217374 44-45 0.00907099069204466 46-47 0.0018981147923882 48-49 3.596428027682905E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.598412456747958E-4 68-69 9.990077854674738E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1988093425609684E-4 82-83 8.791268512113769E-4 84-85 0.0035364875605548565 86-87 6.393649826991832E-4 88-89 3.996031141869895E-5 90-91 0.003496527249136158 92-93 8.391665397926779E-4 94-95 7.99206228373979E-5 96-97 3.596428027682905E-4 98-99 2.7972217993089266E-4 100-101 0.0024375789965406357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2502483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.02955967341186 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03135375577786 37.777873841839295 2 14.387708746609555 14.68396884097564 3 4.987547490101192 7.63537056810283 4 2.264523949513213 4.622306400542192 5 1.190571625386871 3.0377172901575142 6 0.7074839800683901 2.16615575873297 7 0.47596137554503815 1.7001669590930302 8 0.3270980142764667 1.3353334110860384 9 0.23261687671920386 1.0683303112426772 >10 1.2370510560977845 11.631913169191373 >50 0.09791144919422837 3.4429746896579823 >100 0.052952773461304684 5.091055634031515 >500 0.0034525208582164933 1.2503618623744919 >1k 0.0035309872413556128 3.3133968716637123 >5k 7.846638314123585E-5 0.27980685606267097 >10k+ 1.569327662824717E-4 0.9632675352460899 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13106 0.5237198414534685 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10951 0.43760537034617214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6988 0.27924265619386823 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3835 0.15324779429071045 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3631 0.14509589076129586 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3481 0.13910184404849105 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3472 0.13874220124572276 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3435 0.13726366972323087 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2812 0.11236839570938145 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2758 0.1102105388927717 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2644 0.10565506339104001 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2558 0.10221847660903192 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2517 0.10058010384086526 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.996031141869895E-5 2 3.996031141869895E-5 0.0 0.0 0.0 3.996031141869895E-5 3 3.996031141869895E-5 0.0 0.0 3.996031141869895E-5 3.996031141869895E-5 4 3.996031141869895E-5 0.0 0.0 7.99206228373979E-5 3.996031141869895E-5 5 3.996031141869895E-5 0.0 0.0 7.99206228373979E-5 1.1988093425609684E-4 6 7.99206228373979E-5 0.0 0.0 7.99206228373979E-5 1.1988093425609684E-4 7 7.99206228373979E-5 0.0 0.0 7.99206228373979E-5 1.1988093425609684E-4 8 7.99206228373979E-5 0.0 0.0 7.99206228373979E-5 1.1988093425609684E-4 9 7.99206228373979E-5 0.0 0.0 7.99206228373979E-5 1.1988093425609684E-4 10-11 7.99206228373979E-5 0.0 0.0 7.99206228373979E-5 1.598412456747958E-4 12-13 7.99206228373979E-5 0.0 0.0 7.99206228373979E-5 3.596428027682905E-4 14-15 1.1988093425609684E-4 0.0 0.0 7.99206228373979E-5 4.1958326989633896E-4 16-17 1.3986108996544633E-4 0.0 0.0 7.99206228373979E-5 4.395634256056884E-4 18-19 2.1978171280284423E-4 0.0 0.0 7.99206228373979E-5 4.7952373702438736E-4 20-21 2.7972217993089266E-4 0.0 0.0 1.3986108996544633E-4 4.7952373702438736E-4 22-23 2.7972217993089266E-4 0.0 0.0 3.5964280276829056E-4 4.7952373702438736E-4 24-25 3.5964280276829056E-4 0.0 0.0 4.5954358131503786E-4 4.7952373702438736E-4 26-27 3.7962295847764E-4 0.0 0.0 0.00113886887543292 5.194840484430863E-4 28-29 4.5954358131503786E-4 0.0 0.0 0.003456566937717459 5.194840484430863E-4 30-31 4.7952373702438736E-4 0.0 0.0 0.011208867352945056 5.194840484430863E-4 32-33 5.194840484430863E-4 0.0 0.0 0.02777241643599577 5.194840484430863E-4 34-35 6.193848269898338E-4 0.0 0.0 0.05486550757787366 5.594443598617853E-4 36-37 6.793252941178821E-4 0.0 0.0 0.09752314001733478 5.594443598617853E-4 38-39 8.391665397926779E-4 0.0 0.0 0.16241868576130186 5.794245155711348E-4 40-41 8.591466955020274E-4 0.0 0.0 0.23003153268174048 5.994046712804842E-4 42-43 9.590474740487748E-4 0.0 0.0 0.2980040224049474 5.994046712804842E-4 44-45 0.00113886887543292 0.0 0.0 0.3820405573184713 6.593451384085327E-4 46-47 0.0012587498096890168 0.0 0.0 0.48026300278563333 7.192856055365811E-4 48-49 0.0013186902768170653 0.0 0.0 0.5857582249309985 7.192856055365811E-4 50-51 0.0013186902768170653 0.0 0.0 0.7001645965227337 8.391665397926779E-4 52-53 0.0013186902768170653 0.0 0.0 0.808237258754605 8.391665397926779E-4 54-55 0.0013586505882357642 0.0 0.0 0.9218843844293847 8.791268512113768E-4 56-57 0.0013586505882357642 0.0 0.0 1.0559312490834103 9.190871626300757E-4 58-59 0.0013586505882357642 0.0 0.0 1.194034085346434 9.590474740487747E-4 60-61 0.0013586505882357642 0.0 0.0 1.3436654714537521 9.590474740487747E-4 62-63 0.0013586505882357642 0.0 0.0 1.4879821361423833 0.0011988093425609684 64-65 0.0013586505882357642 0.0 0.0 1.6345365782704617 0.0011988093425609684 66-67 0.0013586505882357642 0.0 0.0 1.8074248656234628 0.0011988093425609684 68-69 0.0013586505882357642 0.0 0.0 1.9978357495335635 0.0011988093425609684 70-71 0.0013586505882357642 0.0 0.0 2.196078854481729 0.0011988093425609684 72-73 0.0013986108996544631 0.0 0.0 2.401175152838201 0.0013386704325264148 74-75 0.0014385712110731622 0.0 0.0 2.602295400208513 0.0015384719896199095 76-77 0.0014385712110731622 0.0 0.0 2.8186804865407677 0.0015984124567479578 78-79 0.0014585513667825117 0.0 0.0 3.066873980762307 0.0016183926124573073 80-81 0.001638372768166657 0.0 0.0 3.3246979100357525 0.001638372768166657 82-83 0.001638372768166657 0.0 0.0 3.5907137031500316 0.001638372768166657 84-85 0.0016983132352947053 0.0 0.0 3.8612050511432043 0.001638372768166657 86-87 0.0017782338581321033 0.0 0.0 4.141266893721156 0.0016783330795853558 88-89 0.0017982140138414527 0.0 0.0 4.436693476039597 0.0017182933910040547 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5445 0.0 60.547424 1 GTATCAA 10235 0.0 43.118332 1 TATCAAC 13360 0.0 32.85486 2 ATCAACG 13285 0.0 32.821857 3 TCAACGC 13540 0.0 32.377827 4 CAACGCA 13675 0.0 32.02346 5 AACGCAG 14115 0.0 31.092508 6 TGGTATC 2145 0.0 28.347628 2 ACGCAGA 16200 0.0 26.97351 7 CGCAGAG 16205 0.0 26.84795 8 TAAGGTG 1885 0.0 24.44134 5 GCAGAGT 17795 0.0 24.102074 9 TAAGGTA 975 0.0 23.870203 4 GTACATG 9575 0.0 23.46694 1 GTGGTAT 2700 0.0 23.400328 1 TACATGG 9345 0.0 23.383667 2 GTAAGGT 1890 0.0 23.371456 4 GTGTAAG 1130 0.0 23.121626 1 GAGTACT 11680 0.0 22.650457 12-13 ACATGGG 9445 0.0 22.278387 3 >>END_MODULE