FastQCFastQC Report
Thu 26 May 2016
SRR937917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508512
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140920.5617672947149546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112220.4473568394330982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69150.2756614279700476No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39570.15774291691648276No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37480.1494112844586751No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34210.13637566812516744No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33060.13179127705986657No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31580.125891365080175No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27610.11006524983735377No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.10843081476189868No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27110.10807203633070124No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26580.10595923001364954No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25200.10045796073528848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.056.691311
GTATCAA103550.042.7959821
TATCAAC130500.033.6476942
ATCAACG132050.033.252743
TCAACGC133300.032.7983134
CAACGCA133800.032.6047135
AACGCAG137350.031.8657976
TGGTATC23450.031.6137162
GCGTATC804.3571775E-429.8459281
ACGCAGA158250.027.4296517
CGCAGAG158950.027.2191188
GTGGTAT29200.026.6569141
TAAGGTG18300.025.708575
GTACATG96200.025.31631
GTAAGGT17550.025.1825474
GCAGAGT172400.024.7095019
TACATGG94650.024.501522
GGTAAGG17900.023.8936943
GAGTACT112050.023.54604512-13
ACATGGG96800.022.9263733
Produced by FastQC (version 0.11.5)