FastQCFastQC Report
Thu 26 May 2016
SRR937917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508512
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134100.5345798624842137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110120.4389853427051575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.27968771925348573No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38180.15220178336798867No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36790.1466606498194946No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35730.1424350371853912No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35350.14092019492033522No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34160.1361763467745022No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28740.11456991236238855No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.11317466290773175No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28000.11161995637254277No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26540.1057997729331173No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25350.10105592478728426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.061.52721
GTATCAA103000.042.5273671
ATCAACG132550.032.897273
TATCAAC133400.032.622292
TCAACGC135650.032.1785624
CAACGCA136950.031.8037415
AACGCAG142000.030.7730396
TGGTATC21200.028.4605082
ACGCAGA162150.026.9196557
CGCAGAG162450.026.8699428
GTAAGGT17700.025.224674
TAAGGTG18350.024.3311545
GCAGAGT180400.023.9593939
GTGGTAT26500.023.3062421
GAGTACT117750.022.91129912-13
TACATGG94400.022.6472432
GTACATG96350.022.4847431
GTGTAGC18100.022.31081
CAGAGTA170350.022.29146410-11
AGGTAAG17950.022.2325692
Produced by FastQC (version 0.11.5)