##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937917_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2508512 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50779306616831 34.0 31.0 34.0 31.0 34.0 2 32.73270568368818 34.0 31.0 34.0 31.0 34.0 3 32.783793340434485 34.0 31.0 34.0 31.0 34.0 4 36.24105525506754 37.0 35.0 37.0 35.0 37.0 5 36.205795706777565 37.0 37.0 37.0 35.0 37.0 6 36.24365281090942 37.0 37.0 37.0 35.0 37.0 7 36.20946561148601 37.0 37.0 37.0 35.0 37.0 8 36.201724368868874 37.0 37.0 37.0 35.0 37.0 9 37.91340204870457 39.0 38.0 39.0 35.0 39.0 10-11 37.90127254723119 39.0 38.0 39.0 35.0 39.0 12-13 37.851519745570286 39.0 38.0 39.0 35.0 39.0 14-15 39.17102529308211 40.5 38.5 41.0 36.0 41.0 16-17 39.12808130875993 40.0 38.5 41.0 36.0 41.0 18-19 39.171112595833705 40.0 39.0 41.0 36.0 41.0 20-21 39.104628162033904 40.0 39.0 41.0 36.0 41.0 22-23 38.96770256630225 40.0 38.5 41.0 35.0 41.0 24-25 38.922227400147975 40.0 38.0 41.0 35.0 41.0 26-27 38.88304839681851 40.0 38.0 41.0 35.0 41.0 28-29 38.82800959293796 40.0 38.0 41.0 35.0 41.0 30-31 38.61535603576941 40.0 38.0 41.0 34.5 41.0 32-33 38.41779429398783 40.0 38.0 41.0 34.0 41.0 34-35 38.215480731206384 40.0 38.0 41.0 34.0 41.0 36-37 38.07239032542001 40.0 38.0 41.0 33.5 41.0 38-39 38.027330146317816 40.0 38.0 41.0 33.0 41.0 40-41 37.94635903675167 40.0 38.0 41.0 33.0 41.0 42-43 37.77788186781646 40.0 37.5 41.0 33.0 41.0 44-45 37.552480514344765 40.0 37.0 41.0 33.0 41.0 46-47 37.20304606874514 40.0 37.0 41.0 32.0 41.0 48-49 36.83387083657563 40.0 36.5 41.0 31.0 41.0 50-51 36.51972902661019 40.0 36.0 41.0 30.5 41.0 52-53 36.10412527426618 39.0 35.0 41.0 29.5 41.0 54-55 35.882226594889715 39.0 35.0 41.0 29.0 41.0 56-57 35.64039478383998 39.0 35.0 40.5 28.0 41.0 58-59 35.30192978945287 38.5 35.0 40.0 27.5 41.0 60-61 34.95376203103673 38.0 34.5 40.0 27.0 41.0 62-63 34.59492021564976 37.5 34.0 40.0 26.0 41.0 64-65 34.21156546191527 37.0 34.0 40.0 26.0 41.0 66-67 33.799207857088184 36.0 33.5 39.0 25.5 41.0 68-69 33.35496521443788 36.0 33.0 39.0 24.5 40.5 70-71 32.844554859613986 35.0 32.5 38.5 22.5 40.0 72-73 32.441104726626776 35.0 32.0 37.5 22.0 40.0 74-75 32.00704421585386 35.0 32.0 37.0 20.5 39.0 76-77 30.709430730249647 34.0 30.0 35.5 20.0 38.5 78-79 31.41975621404243 35.0 31.5 36.0 20.0 39.0 80-81 31.26038823015397 35.0 32.0 36.0 19.5 37.5 82-83 30.886799225995333 35.0 31.0 35.5 18.0 37.0 84-85 30.50914506288987 34.0 31.0 35.0 15.5 37.0 86-87 30.085732697312192 34.0 31.0 35.0 8.5 36.0 88-89 29.617124414792514 34.0 30.0 35.0 4.5 36.0 90-91 29.27569770445587 34.0 30.0 35.0 2.0 35.5 92-93 28.87382599724458 34.0 29.0 35.0 2.0 35.0 94-95 28.46691046325471 34.0 29.0 35.0 2.0 35.0 96-97 27.898424444451532 34.0 29.0 35.0 2.0 35.0 98-99 27.222383428901274 33.5 27.0 35.0 2.0 35.0 100-101 24.804953295021114 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07990687663910734 1101 2 0.15384015610342772 1101 3 0.17006113287775548 1101 4 0.05639251324925709 1101 5 0.07675596594491907 1101 6 0.04078468672616253 1101 7 0.10572782268128122 1101 8 0.023754390068646103 1101 9 0.011351021584744103 1101 10-11 0.10802067384180702 1101 12-13 0.11824192002638512 1101 14-15 0.07023052146759312 1101 16-17 0.11086127303147464 1101 18-19 0.17322121307176985 1101 20-21 0.17761354071012647 1101 22-23 0.17068784299422646 1101 24-25 0.13693959618867524 1101 26-27 0.1996433158973261 1101 28-29 0.13063384732918593 1101 30-31 0.12453509267840701 1101 32-33 0.14387517808064132 1101 34-35 0.13493615768936706 1101 36-37 0.22278849808964907 1101 38-39 0.22416954513592913 1101 40-41 0.39083098085256296 1101 42-43 0.3542052454661331 1101 44-45 0.4954947590208434 1101 46-47 0.4879930904729335 1101 48-49 0.41340809650591126 1101 50-51 0.47074848524836455 1101 52-53 0.4290604222362191 1101 54-55 0.41335140483730015 1101 56-57 0.5091280862449352 1101 58-59 0.4786327735391396 1101 60-61 0.49380685969582316 1101 62-63 0.39314643164230034 1101 64-65 0.42586684458111534 1101 66-67 0.3235231151050968 1101 68-69 0.47133181699750537 1101 70-71 0.4017055133581806 1101 72-73 0.34393570239400617 1101 74-75 0.46173971058545504 1101 76-77 0.3940853595304752 1101 78-79 0.382498392588527 1101 80-81 0.45978920343549134 1101 82-83 0.5218947675744907 1101 84-85 0.4907555362373479 1101 86-87 0.4403789046961286 1101 88-89 0.5539270418704767 1101 90-91 0.5968215762420108 1101 92-93 0.6101573088571719 1101 94-95 0.4001430976295737 1101 96-97 0.4904750414481356 1101 98-99 0.5714609701242139 1101 100-101 0.7330428914293456 1102 1 0.11538728889970429 1102 2 0.1456458736057371 1102 3 0.14742566720823902 1102 4 0.1183455241186735 1102 5 0.06428628907895728 1102 6 0.009512656382923979 1102 7 0.13751048790047804 1102 8 0.12006200402331757 1102 9 0.0747099536743292 1102 10-11 0.11722550409926669 1102 12-13 0.17563272323761936 1102 14-15 0.27653371260050363 1102 16-17 0.23380700634871943 1102 18-19 0.22648203893321295 1102 20-21 0.20054855203522948 1102 22-23 0.23582595565556375 1102 24-25 0.12154348559132444 1102 26-27 0.057187577621157004 1102 28-29 0.08521548859813777 1102 30-31 0.09902948373626685 1102 32-33 0.08144838918327224 1102 34-35 0.22921932550194413 1102 36-37 0.27542476790858217 1102 38-39 0.18172050810485274 1102 40-41 0.37924586833865703 1102 42-43 0.21066591585868366 1102 44-45 0.23575852002764464 1102 46-47 0.19195486657093142 1102 48-49 0.2258476177689559 1102 50-51 0.2827383529657794 1102 52-53 0.2484350620442939 1102 54-55 0.09651653691884121 1102 56-57 0.3617543041962321 1102 58-59 0.2746850700558383 1102 60-61 0.21069198695246882 1102 62-63 0.1412875062539598 1102 64-65 0.36365650590018817 1102 66-67 0.30056867016922695 1102 68-69 0.4433621602352815 1102 70-71 0.3628784992989722 1102 72-73 0.25802842082109123 1102 74-75 0.2754779331472079 1102 76-77 0.24356601303589542 1102 78-79 0.22757766831795934 1102 80-81 0.4598836909912798 1102 82-83 0.5325222721648437 1102 84-85 0.41329919484912025 1102 86-87 0.2629252438059346 1102 88-89 0.014145100385750453 1102 90-91 0.005871607737862661 1102 92-93 0.09374394925495722 1102 94-95 -0.06616907317176057 1102 96-97 -0.13137838891922726 1102 98-99 -0.17708821031351007 1102 100-101 -0.06442202755259885 1103 1 0.16448836641912834 1103 2 0.17760851019760793 1103 3 0.18828475097345176 1103 4 0.1091670329427572 1103 5 0.14993006379260265 1103 6 0.12354434551105697 1103 7 0.1469211087127249 1103 8 0.12536554460332638 1103 9 0.1956355775575176 1103 10-11 0.11762945791085144 1103 12-13 0.1727109700372509 1103 14-15 0.3341488078924115 1103 16-17 0.33305337364053145 1103 18-19 0.2436599103545447 1103 20-21 0.28334191954785126 1103 22-23 0.24674189355392429 1103 24-25 0.2965030809324034 1103 26-27 0.2679162902572827 1103 28-29 0.20054049792228312 1103 30-31 0.21594238223939755 1103 32-33 0.37235979953316445 1103 34-35 0.2641617013144426 1103 36-37 0.22732819381544545 1103 38-39 0.27252522417746405 1103 40-41 0.3242368967416098 1103 42-43 0.2989557086491246 1103 44-45 0.24671871285667635 1103 46-47 0.2742315554485657 1103 48-49 0.3554328275299099 1103 50-51 0.33703831789583916 1103 52-53 0.35380818584862084 1103 54-55 0.379542126845422 1103 56-57 0.36085436116169234 1103 58-59 0.34650822475511234 1103 60-61 0.21367209728362724 1103 62-63 0.33648227433771893 1103 64-65 0.2689494698301047 1103 66-67 0.27943707994204914 1103 68-69 0.403333065169754 1103 70-71 0.39509924326424795 1103 72-73 0.2144921456945923 1103 74-75 0.3069240766593637 1103 76-77 0.2972679333473316 1103 78-79 0.1804644443508998 1103 80-81 0.21789355510916053 1103 82-83 0.252036098815406 1103 84-85 0.33413807164599163 1103 86-87 0.3618027272312112 1103 88-89 0.22192014431595553 1103 90-91 0.2891394400627014 1103 92-93 0.4330719237522409 1103 94-95 0.3305118070281914 1103 96-97 0.4571015052400469 1103 98-99 0.5085551932895989 1103 100-101 0.7000874663825272 1104 1 0.10522167828271733 1104 2 0.024671074912433255 1104 3 0.0429168946239642 1104 4 0.05701737066905821 1104 5 -0.008834981662339203 1104 6 -0.03272453468479597 1104 7 0.035982990640519574 1104 8 0.01825099872441882 1104 9 0.009736519230230556 1104 10-11 0.09216239869147813 1104 12-13 0.12720915938525224 1104 14-15 0.18094816014175308 1104 16-17 0.08476961917018144 1104 18-19 0.1341402198987396 1104 20-21 0.0642963826480667 1104 22-23 0.09557500840830357 1104 24-25 0.10562857708306694 1104 26-27 0.11104069345622491 1104 28-29 0.14146359010680243 1104 30-31 0.12305652358194408 1104 32-33 0.18785920583208338 1104 34-35 0.26497446763788446 1104 36-37 0.27159643238143616 1104 38-39 0.34238883034477396 1104 40-41 0.29809364619340784 1104 42-43 0.2943302750257146 1104 44-45 0.3277712672311992 1104 46-47 0.4368361786660273 1104 48-49 0.4079305669631097 1104 50-51 0.4752253899337404 1104 52-53 0.5284587587688492 1104 54-55 0.5502093357080682 1104 56-57 0.5209460220666458 1104 58-59 0.4641831045066809 1104 60-61 0.6155550557671177 1104 62-63 0.591644378215264 1104 64-65 0.6154924691436179 1104 66-67 0.47344515765383477 1104 68-69 0.6482066660235759 1104 70-71 0.5173042267653187 1104 72-73 0.463717766354506 1104 74-75 0.424578026051158 1104 76-77 0.4513392137102059 1104 78-79 0.6166389831486647 1104 80-81 0.5276077396139733 1104 82-83 0.5406215248476016 1104 84-85 0.6943962608251333 1104 86-87 0.6037400096719914 1104 88-89 0.5722060723777851 1104 90-91 0.5644916021600714 1104 92-93 0.7115531746349681 1104 94-95 0.4731428869239487 1104 96-97 0.6048492666149521 1104 98-99 0.6264829962121468 1104 100-101 0.7090393284897978 1105 1 0.10278740713683021 1105 2 0.052688610604420205 1105 3 0.06283082005832341 1105 4 0.08574449812557816 1105 5 0.006475964308279458 1105 6 0.013721516538922174 1105 7 0.0011830854937784352 1105 8 -0.013702322692552116 1105 9 0.027842283899168763 1105 10-11 -0.004133459990974586 1105 12-13 0.048825316254003326 1105 14-15 0.08207941506250904 1105 16-17 -0.050041230583211416 1105 18-19 0.020185912152498986 1105 20-21 0.07691924448522514 1105 22-23 0.1044997928315965 1105 24-25 0.05437240410306288 1105 26-27 0.06847636225796094 1105 28-29 0.07865904944257807 1105 30-31 0.12921713749220487 1105 32-33 0.0762545375136412 1105 34-35 0.14503735475609858 1105 36-37 0.21365254600420513 1105 38-39 0.1453196842590927 1105 40-41 0.14107625527917378 1105 42-43 0.20018858115227545 1105 44-45 0.1184806437189252 1105 46-47 0.11681136103095469 1105 48-49 0.15450581713200506 1105 50-51 0.060071152325015476 1105 52-53 0.16798614238832243 1105 54-55 0.1389388722927194 1105 56-57 0.22266358581035917 1105 58-59 0.1536417228010265 1105 60-61 0.08357659133486806 1105 62-63 0.10159320642750203 1105 64-65 0.06575235425154347 1105 66-67 0.12047255483081898 1105 68-69 0.16960698736117052 1105 70-71 0.29749527752807836 1105 72-73 0.2549618838338503 1105 74-75 0.3357683769178337 1105 76-77 0.25573871551696925 1105 78-79 0.30416290393403145 1105 80-81 0.3604987147340566 1105 82-83 0.3750466347332626 1105 84-85 0.43423384023310163 1105 86-87 0.43167345594621764 1105 88-89 0.31510148614389877 1105 90-91 0.24677879159659 1105 92-93 0.5059595603692486 1105 94-95 0.47284059557359015 1105 96-97 0.7136480237283322 1105 98-99 0.6195344034897197 1105 100-101 0.6466455392418418 1106 1 -0.20895377088439204 1106 2 -0.07929556154139306 1106 3 -0.07308401825397937 1106 4 -0.08448065813788475 1106 5 -0.06534170423574182 1106 6 -0.017990905456080952 1106 7 -0.016070530237506375 1106 8 -0.170004917451827 1106 9 -0.14754777277643427 1106 10-11 -0.14613875558619327 1106 12-13 -0.17849644137414344 1106 14-15 -0.24950178934480505 1106 16-17 -0.17488336709639185 1106 18-19 -0.11442910688299435 1106 20-21 -0.1434386184946561 1106 22-23 -0.05302103596041974 1106 24-25 -0.10432847999582862 1106 26-27 -0.11935069159907385 1106 28-29 -0.10180109450230646 1106 30-31 -0.08518215114783345 1106 32-33 -0.23139906136196942 1106 34-35 -0.11426439278399414 1106 36-37 -0.22608233522035448 1106 38-39 -0.2305966051616295 1106 40-41 -0.23992778501221323 1106 42-43 -0.2627077963995177 1106 44-45 -0.16107024456219676 1106 46-47 -0.2419862055426023 1106 48-49 -0.2539394596051423 1106 50-51 -0.1873421993932496 1106 52-53 -0.2691224068581164 1106 54-55 -0.6141910703473172 1106 56-57 -0.4263959026644173 1106 58-59 -0.36271817806735385 1106 60-61 -0.34839121623674885 1106 62-63 -0.15941612819175788 1106 64-65 -0.4183834346444968 1106 66-67 -0.5371686716029274 1106 68-69 -0.4318452141413971 1106 70-71 -0.49521903344255236 1106 72-73 -0.3522630677536185 1106 74-75 -0.40703754715308094 1106 76-77 -0.3151937135244651 1106 78-79 -0.419918256116258 1106 80-81 -0.48967655888542794 1106 82-83 -0.7698319664235704 1106 84-85 -0.5729654634883268 1106 86-87 -0.3766507261102241 1106 88-89 -0.3123608040473691 1106 90-91 -0.4215014674991373 1106 92-93 -0.4394121483394322 1106 94-95 -0.4963335684865733 1106 96-97 -0.4093977324449014 1106 98-99 -0.28298699243855907 1106 100-101 -0.11225255936256318 1107 1 0.07572767642382416 1107 2 0.10597876360730396 1107 3 0.062305641651462906 1107 4 0.07746935711298875 1107 5 0.024690894513682338 1107 6 0.029196260930987705 1107 7 -0.0036887245336743035 1107 8 0.023776537481829507 1107 9 -0.05224885558870085 1107 10-11 0.047237597929132846 1107 12-13 0.09156561063134916 1107 14-15 0.21413916688518242 1107 16-17 0.11342254643113137 1107 18-19 0.07690744184355225 1107 20-21 -0.021765216024022038 1107 22-23 0.06914087116174272 1107 24-25 0.034628455340453 1107 26-27 0.017654608080654555 1107 28-29 -0.07315565832723081 1107 30-31 0.012108911838289771 1107 32-33 0.061091311521970226 1107 34-35 0.15649824638026644 1107 36-37 0.1380013730303702 1107 38-39 0.027159895524654587 1107 40-41 0.150187263790599 1107 42-43 0.08574138005312903 1107 44-45 -0.03316066319877109 1107 46-47 0.0793259417808656 1107 48-49 -0.028007249753081 1107 50-51 0.06131858297673887 1107 52-53 0.022598273330999064 1107 54-55 0.18047493544801796 1107 56-57 0.22131837215638228 1107 58-59 0.145305212965134 1107 60-61 -0.011027520949269842 1107 62-63 0.09087220870011237 1107 64-65 0.15682006924447478 1107 66-67 0.04394216414512897 1107 68-69 0.22399491854123 1107 70-71 0.19151365681214116 1107 72-73 0.06892802573425172 1107 74-75 0.1255040114865409 1107 76-77 0.013834424343464491 1107 78-79 0.248332277664435 1107 80-81 0.29246466423490247 1107 82-83 0.18914079169752185 1107 84-85 0.4358276202389888 1107 86-87 0.4082022935634093 1107 88-89 0.36258105198434976 1107 90-91 0.45435401137839904 1107 92-93 0.537761578212347 1107 94-95 0.611139837796788 1107 96-97 0.8025703932223252 1107 98-99 0.7346325363093449 1107 100-101 0.6286326587249782 1108 1 0.00964815332002189 1108 2 0.05953615947780122 1108 3 0.06339170572552888 1108 4 -0.02460036825774381 1108 5 7.929107792037371E-4 1108 6 0.0121642947976639 1108 7 0.0022295956719773358 1108 8 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-0.5627968488048438 2316 98-99 -0.49764782101910754 2316 100-101 -0.3511079693480319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 6.0 4 13.0 5 41.0 6 87.0 7 209.0 8 547.0 9 1080.0 10 1836.0 11 3068.0 12 4768.0 13 6839.0 14 9496.0 15 12902.0 16 16650.0 17 24012.0 18 23700.0 19 16720.0 20 13769.0 21 13269.0 22 13348.0 23 14315.0 24 16184.0 25 18674.0 26 22245.0 27 26485.0 28 32562.0 29 39858.0 30 48507.0 31 60200.0 32 75523.0 33 98266.0 34 138022.0 35 212148.0 36 352662.0 37 582277.0 38 537783.0 39 70422.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13134639180518 19.089165210292 15.144874730517536 22.63461366738529 2 15.697979242611696 20.165869322787316 40.07844125418292 24.057710180418074 3 19.36785460703802 23.9614774191928 30.09149053141372 26.579177442355462 4 12.886523963210061 16.994497136150834 36.8169655955403 33.3020133050988 5 13.412378897282093 37.5439055280204 34.28822915267264 14.755486422024857 6 32.10668316515927 36.38850442015028 16.999759219808396 14.505053194882064 7 28.426652932096797 33.37325075582656 20.008634600910817 18.191461711165825 8 26.640600645325378 34.75177615963631 19.431557160408882 19.176066034629432 9 26.260933833712446 16.747910502619494 20.22425371676597 36.766901946902095 10-11 25.028582681685396 26.824926490285872 26.73068735569134 21.415803472337387 12-13 26.09032765240908 24.094483103927747 27.42129597147632 22.393893272186858 14-15 22.506071328341264 25.48676267045962 26.20573471444426 25.801431286754855 16-17 21.687497446190612 28.736788708026584 27.195174820087242 22.38053902569556 18-19 21.733142994731537 26.700988474442216 30.049906079779564 21.515962451046676 20-21 22.182413319131022 25.824094124325498 30.52435069076807 21.469141865775406 22-23 21.947891817938284 25.816001677488487 30.496186583919073 21.739919920654156 24-25 21.759032426917944 26.020190728753512 30.534859365351092 21.68591747897746 26-27 21.243329324814432 26.44591769032756 30.267301490594868 22.043451494263138 28-29 21.451565998401843 26.910939017022645 30.03247144770913 21.605023536866387 30-31 21.5829344248702 26.35133896110523 29.981359467285785 22.084367146738785 32-33 21.159221718778777 26.332468540985815 30.100195255973482 22.408114484261922 34-35 22.029279631746878 26.604055130314997 29.769632474534575 21.59703276340355 36-37 22.20525725675844 26.005830322661016 29.825299613803413 21.963612806777128 38-39 21.616680827582247 26.435173242288812 29.750222979597517 22.197922950531424 40-41 22.178464661832802 26.290598842647416 29.721311187821403 21.80962530769838 42-43 21.820308198684614 26.249732390284002 29.59342858179409 22.336530829237294 44-45 21.763324316475178 26.68253835686148 29.71616530450384 21.837972022159505 46-47 22.310497219068516 26.962996389891696 28.83874982459721 21.887756566442576 48-49 22.036330701228458 27.015716887142656 28.721409345460575 22.22654306616831 50-51 22.25773685754742 27.23977401742547 28.6899564363256 21.81253268870151 52-53 22.653110688727022 26.916335261700965 28.55525506754602 21.875298982026 54-55 21.939340931994742 27.262177737240243 28.709350403745326 22.089130927019685 56-57 22.113507928206044 27.34264376650381 28.478237297648967 22.065611007641184 58-59 22.027919340230383 26.977786034111055 28.42697184625786 22.567322779400698 60-61 21.631349580946793 27.23485078006404 28.804984787794517 22.328814851194654 62-63 21.842450823436362 27.20419515633172 28.898326976311058 22.05502704392086 64-65 21.5500663341455 27.275014032223087 28.713137509407964 22.461782124223443 66-67 21.48197816075825 27.347028836218445 28.679193083389677 22.491799919633632 68-69 22.18379741092957 26.888947666669388 28.734348505774253 22.192906416626787 70-71 21.918591579390494 27.126081119006006 28.265282366598203 22.690044935005293 72-73 21.833042855684965 27.313184070875486 28.301977427255682 22.551795646183873 74-75 21.914844337997984 27.645213576813667 28.084418173004554 22.3555239121838 76-77 21.94225102371446 27.258011921011338 28.292828577260142 22.506908478014058 78-79 21.992220886326237 27.598691973568396 28.170584792897145 22.238502347208225 80-81 22.13296169203097 27.61029247617711 27.87744288247375 22.379302949318163 82-83 22.112349562090643 27.725362864808194 27.901264097013765 22.2610234760874 84-85 22.047372479982076 27.88847408916978 27.835532808345587 22.228620622502557 86-87 22.269731734478345 27.844709006784324 27.509529087760892 22.376030170976442 88-89 22.052046014548072 27.938625742522156 27.405680102658508 22.60364814027126 90-91 21.938613611937633 27.990088913551663 27.55728115243189 22.514016322078817 92-93 22.021095424926234 27.622836062580674 27.705953264612482 22.650115247880613 94-95 21.802048385656516 27.458848113941652 27.947883047798854 22.79122045260298 96-97 22.135632598129888 27.0515349338572 27.956394069472264 22.85643839854065 98-99 21.777910570090956 27.406685716472555 27.53793882588563 23.277464887550867 100-101 21.82624599762728 27.201783367988675 27.95033470041204 23.02163593397201 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 993.0 1 682.0 2 548.5 3 749.5 4 618.5 5 561.0 6 1137.5 7 2537.5 8 4126.0 9 4785.0 10 4004.5 11 2998.5 12 2854.0 13 2990.5 14 3140.0 15 3313.0 16 3509.0 17 3842.0 18 4218.0 19 4597.0 20 5153.5 21 6082.5 22 7471.0 23 9169.5 24 11335.5 25 14383.5 26 18400.0 27 22926.5 28 26423.0 29 29788.0 30 34007.5 31 39609.5 32 48008.0 33 51599.0 34 51140.5 35 53126.0 36 56873.5 37 60747.0 38 63624.5 39 66775.5 40 70590.5 41 74863.5 42 76808.0 43 78698.0 44 82005.0 45 86225.0 46 87728.5 47 89652.5 48 91753.0 49 89320.5 50 86967.5 51 83966.0 52 81691.0 53 82701.0 54 85535.0 55 81032.5 56 72558.5 57 66405.5 58 62450.0 59 55946.0 60 44088.5 61 36237.5 62 31245.0 63 23015.0 64 15011.0 65 10843.5 66 8070.0 67 6183.0 68 4806.5 69 3873.0 70 3031.0 71 1916.5 72 1343.5 73 1123.0 74 817.5 75 578.0 76 423.0 77 273.5 78 165.0 79 90.0 80 44.5 81 31.0 82 21.0 83 9.0 84 6.5 85 5.5 86 4.5 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01566665816228904 3 0.006577604571953413 4 0.0 5 3.986427013305099E-5 6 0.0 7 0.0 8 3.986427013305099E-4 9 5.580997818627138E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.7938921559872945E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005301947927695782 26-27 0.020729420469186514 28-29 1.9932135066525494E-5 30-31 0.0 32-33 3.986427013305099E-4 34-35 0.01811831077547167 36-37 0.0029698881249122988 38-39 0.01734095750787718 40-41 0.01490923702976107 42-43 0.007773532675944943 44-45 0.0024915168833156867 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.1925348573178046E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.972854026610198E-5 84-85 0.0028901595846461967 86-87 3.388462961309334E-4 88-89 3.986427013305099E-5 90-91 3.189141610644079E-4 92-93 2.3918562079830593E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2508512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.87304711276195 #Duplication Level Percentage of deduplicated Percentage of total 1 73.83218050823885 37.5606799743358 2 14.646334883403345 14.902073691053344 3 4.963965616155286 7.575961699703948 4 2.22027784600869 4.518091978536869 5 1.158089707775236 2.9457776132227154 6 0.7198379944413071 2.197221132286122 7 0.4821181493441172 1.7168773527850616 8 0.32424222608941494 1.3196152035034894 9 0.23953475924256037 1.0967276782881779 >10 1.2547595282666624 11.723113751779742 >50 0.09734557905969252 3.430284532993444 >100 0.053629156120127384 5.032679630589907 >500 0.003998840189696744 1.4126240836431472 >1k 0.0034499797715002146 3.3142457005022212 >5k 7.840863117045943E-5 0.27986024088875017 >10k+ 1.5681726234091886E-4 0.9741657358872656 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13410 0.5345798624842137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11012 0.4389853427051575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7016 0.27968771925348573 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3818 0.15220178336798867 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3679 0.1466606498194946 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3573 0.1424350371853912 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3535 0.14092019492033522 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3416 0.1361763467745022 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2874 0.11456991236238855 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2839 0.11317466290773175 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2800 0.11161995637254277 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2654 0.1057997729331173 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2535 0.10105592478728426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.972854026610198E-5 2 0.0 0.0 0.0 0.0 7.972854026610198E-5 3 0.0 0.0 0.0 0.0 7.972854026610198E-5 4 0.0 0.0 0.0 0.0 7.972854026610198E-5 5 0.0 0.0 0.0 0.0 7.972854026610198E-5 6 0.0 0.0 0.0 0.0 7.972854026610198E-5 7 3.986427013305099E-5 0.0 0.0 0.0 7.972854026610198E-5 8 3.986427013305099E-5 0.0 0.0 0.0 7.972854026610198E-5 9 3.986427013305099E-5 0.0 0.0 0.0 7.972854026610198E-5 10-11 7.972854026610198E-5 0.0 0.0 0.0 1.1959281039915297E-4 12-13 7.972854026610198E-5 0.0 0.0 0.0 2.3918562079830593E-4 14-15 1.5945708053220395E-4 0.0 0.0 0.0 2.989820259978824E-4 16-17 1.5945708053220395E-4 0.0 0.0 0.0 3.587784311974589E-4 18-19 1.5945708053220395E-4 0.0 0.0 0.0 3.986427013305099E-4 20-21 1.5945708053220395E-4 0.0 0.0 7.972854026610198E-5 4.5843910653008634E-4 22-23 1.5945708053220395E-4 0.0 0.0 2.3918562079830593E-4 5.182355117296628E-4 24-25 2.3918562079830593E-4 0.0 0.0 3.3884629613093343E-4 5.182355117296628E-4 26-27 2.3918562079830593E-4 0.0 0.0 9.766746182597492E-4 5.979640519957648E-4 28-29 2.5911775586483145E-4 0.0 0.0 0.003826969932772895 5.979640519957648E-4 30-31 2.790498909313569E-4 0.0 0.0 0.011979213174981821 6.178961870622903E-4 32-33 2.989820259978824E-4 0.0 0.0 0.02800464976846832 6.378283221288158E-4 34-35 3.7871056626398436E-4 0.0 0.0 0.05451438940694723 6.776925922618667E-4 36-37 5.182355117296628E-4 0.0 0.0 0.09794651171690627 6.776925922618667E-4 38-39 5.979640519957648E-4 0.0 0.0 0.16392187878710568 6.776925922618667E-4 40-41 5.979640519957648E-4 0.0 0.0 0.23577722570193005 7.574211325279687E-4 42-43 7.374889974614432E-4 0.0 0.0 0.3060380018114324 7.574211325279687E-4 44-45 9.168782130601727E-4 0.0 0.0 0.39346034621321324 7.972854026610198E-4 46-47 0.0010962674286589022 0.0 0.0 0.496090909670753 8.371496727940707E-4 48-49 0.0011560638338584787 0.0 0.0 0.6012927185518746 9.168782130601727E-4 50-51 0.0011560638338584787 0.0 0.0 0.7165203913714584 9.567424831932237E-4 52-53 0.0011560638338584787 0.0 0.0 0.8241937850008292 0.0010763352935923766 54-55 0.0011560638338584787 0.0 0.0 0.936650891046166 0.0010763352935923766 56-57 0.0012357923741245806 0.0 0.0 1.0674654934877728 0.0010763352935923766 58-59 0.0012357923741245806 0.0 0.0 1.2102991733744943 0.0010763352935923766 60-61 0.0012357923741245806 0.0 0.0 1.3634576992256764 0.0010763352935923766 62-63 0.0012357923741245806 0.0 0.0 1.5107761095023664 0.0011959281039915295 64-65 0.0012357923741245806 0.0 0.0 1.6596890905843784 0.001215860239058055 66-67 0.0013155209143906827 0.0 0.0 1.8292118993251776 0.0012557245091911061 68-69 0.0013155209143906827 0.0 0.0 2.018726639537702 0.0012756566442576316 70-71 0.0013354530494572082 0.0 0.0 2.2179483295276246 0.0012756566442576316 72-73 0.001375317319590259 0.0 0.0 2.4242459274661634 0.0013354530494572082 74-75 0.0013952494546567845 0.0 0.0 2.6281716013317853 0.0013952494546567845 76-77 0.0013952494546567845 0.0 0.0 2.8513915819418045 0.0013952494546567845 78-79 0.0013952494546567845 0.0 0.0 3.1013006914059016 0.001455045859856361 80-81 0.0015148422650559375 0.0 0.0 3.3609366827824623 0.0014749779949228866 82-83 0.0015148422650559375 0.0 0.0 3.629402609993494 0.0015148422650559375 84-85 0.0015148422650559375 0.0 0.0 3.900678968248906 0.0015148422650559375 86-87 0.0015547065351889885 0.0 0.0 4.186725038588613 0.001654367210521616 88-89 0.0015547065351889885 0.0 0.0 4.487122246176219 0.0017938921559872945 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 61.5272 1 GTATCAA 10300 0.0 42.527367 1 ATCAACG 13255 0.0 32.89727 3 TATCAAC 13340 0.0 32.62229 2 TCAACGC 13565 0.0 32.178562 4 CAACGCA 13695 0.0 31.803741 5 AACGCAG 14200 0.0 30.773039 6 TGGTATC 2120 0.0 28.460508 2 ACGCAGA 16215 0.0 26.919655 7 CGCAGAG 16245 0.0 26.869942 8 GTAAGGT 1770 0.0 25.22467 4 TAAGGTG 1835 0.0 24.331154 5 GCAGAGT 18040 0.0 23.959393 9 GTGGTAT 2650 0.0 23.306242 1 GAGTACT 11775 0.0 22.911299 12-13 TACATGG 9440 0.0 22.647243 2 GTACATG 9635 0.0 22.484743 1 GTGTAGC 1810 0.0 22.3108 1 CAGAGTA 17035 0.0 22.291464 10-11 AGGTAAG 1795 0.0 22.232569 2 >>END_MODULE