FastQCFastQC Report
Thu 26 May 2016
SRR937916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546942
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134890.5296155153906136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106920.41979754544862036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65550.25736746262773164No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38980.15304628059845884No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37620.147706543768959No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35000.13741969781801078No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34710.13628107746466153No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32530.12772179342913972No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.11390129810572835No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28560.11213447341949678No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27840.10930755392152629No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26690.10479233527893451No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25730.10102310928164049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.057.644921
GTATCAA108650.044.093141
TATCAAC136750.034.4995962
ATCAACG136850.034.3360523
TCAACGC139450.033.7640764
CAACGCA141000.033.3254555
AACGCAG143750.032.7482876
TGGTATC23550.030.2921452
ACGCAGA165050.028.398447
CGCAGAG166300.028.0112658
TAAGGTG19000.027.7848195
GTGGTAT27350.027.336641
GTAAGGT18150.026.7277094
GCAGAGT181550.025.2389439
GGTAAGG19600.024.5077533
GAGTACT117050.024.04128812-13
AGGTAAG17250.023.986112
GTGTAGC16700.023.9533141
GTACATG101500.023.881111
TACATGG98200.023.8761922
Produced by FastQC (version 0.11.5)