##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937916_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2546942 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.006801489786575 33.0 31.0 34.0 30.0 34.0 2 32.13020987521506 34.0 31.0 34.0 30.0 34.0 3 32.15081340682277 34.0 31.0 34.0 30.0 34.0 4 35.6665267603267 37.0 35.0 37.0 35.0 37.0 5 35.67498396115813 37.0 35.0 37.0 35.0 37.0 6 35.703026609950285 37.0 36.0 37.0 35.0 37.0 7 35.642792808002696 37.0 35.0 37.0 33.0 37.0 8 35.63137362374172 37.0 35.0 37.0 33.0 37.0 9 37.2769937438701 39.0 37.0 39.0 34.0 39.0 10-11 37.18518089536393 39.0 37.0 39.0 34.0 39.0 12-13 37.041157788438056 39.0 37.0 39.0 33.0 39.0 14-15 38.22533924997114 40.0 38.0 41.0 33.0 41.0 16-17 38.04967388342569 40.0 38.0 41.0 33.0 41.0 18-19 38.247173473129735 40.0 38.0 41.0 33.5 41.0 20-21 38.25020770005756 40.0 38.0 41.0 34.0 41.0 22-23 38.1418836785447 40.0 38.0 41.0 33.5 41.0 24-25 38.02359378423223 40.0 38.0 41.0 33.0 41.0 26-27 37.8395175469249 40.0 38.0 41.0 33.0 41.0 28-29 37.66185076063766 40.0 38.0 41.0 32.0 41.0 30-31 37.420154444035234 40.0 37.0 41.0 32.0 41.0 32-33 37.10743511238183 40.0 36.5 41.0 31.0 41.0 34-35 36.86201609616552 39.5 36.0 41.0 30.5 41.0 36-37 36.582273565711354 39.0 35.5 41.0 30.0 41.0 38-39 36.26456629165486 39.0 35.0 40.5 30.0 41.0 40-41 36.12331945525261 39.0 35.0 41.0 29.5 41.0 42-43 35.8837837689276 39.0 35.0 41.0 28.5 41.0 44-45 35.539721359968155 39.0 35.0 40.0 27.0 41.0 46-47 35.12827971740228 38.5 35.0 40.0 25.5 41.0 48-49 34.68481653685086 38.0 34.0 40.0 24.0 41.0 50-51 33.932850649916645 37.5 33.5 39.5 23.0 40.5 52-53 33.98357579403064 38.0 33.5 40.0 23.0 40.5 54-55 34.05835723781696 38.0 34.0 40.0 22.5 41.0 56-57 33.74151825993682 38.0 33.0 40.0 19.5 41.0 58-59 33.32607083317956 37.5 33.0 40.0 17.5 41.0 60-61 32.82199771333623 37.0 32.5 40.0 12.5 41.0 62-63 32.2884730001704 36.0 31.5 39.0 8.0 41.0 64-65 31.746376046254685 35.5 31.0 39.0 6.5 40.5 66-67 31.175463359589656 35.0 30.5 39.0 2.0 40.0 68-69 30.498776768375564 35.0 29.5 38.0 2.0 40.0 70-71 29.89924466281525 34.5 29.0 37.5 2.0 40.0 72-73 29.209806308899065 34.0 28.0 37.0 2.0 39.0 74-75 28.54402298913756 34.0 26.5 36.0 2.0 39.0 76-77 27.796206784449744 33.5 26.0 35.5 2.0 38.5 78-79 27.035243833585533 33.0 24.5 35.0 2.0 37.0 80-81 26.312414063610397 32.5 22.5 35.0 2.0 37.0 82-83 25.568610906726576 32.0 20.0 35.0 2.0 36.0 84-85 24.84141982817041 31.0 17.5 34.5 2.0 36.0 86-87 24.09783379440914 31.0 9.0 34.0 2.0 35.0 88-89 23.20907798450063 30.5 2.0 34.0 2.0 35.0 90-91 22.30307521726054 29.5 2.0 34.0 2.0 35.0 92-93 21.3631537349496 29.0 2.0 34.0 2.0 35.0 94-95 20.272041530588446 28.0 2.0 34.0 2.0 35.0 96-97 19.005346607814392 25.5 2.0 33.5 2.0 35.0 98-99 17.432884612213392 21.5 2.0 33.0 2.0 34.5 100-101 13.481066314034635 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15394893839877 1101 2 0.2536174631531054 1101 3 0.1622054308114258 1101 4 -0.014792422078002687 1101 5 0.06611979727409789 1101 6 0.14275290188932388 1101 7 0.12090276975025205 1101 8 0.12759375528312233 1101 9 0.19731152181164902 1101 10-11 0.16380698046129538 1101 12-13 0.2066751769162991 1101 14-15 0.21589950147736658 1101 16-17 0.2029199904480734 1101 18-19 0.20432199362675618 1101 20-21 0.2832726220713724 1101 22-23 0.22806544768231163 1101 24-25 0.1839101206295055 1101 26-27 0.06706567294147447 1101 28-29 0.05313002798677502 1101 30-31 0.08677563872414851 1101 32-33 0.15927744859438775 1101 34-35 0.1465459041832915 1101 36-37 0.17657946824411397 1101 38-39 0.21642057628375966 1101 40-41 0.3816924881673174 1101 42-43 0.27930464908745023 1101 44-45 0.22199411021943405 1101 46-47 0.35594313656593357 1101 48-49 0.26026845825946054 1101 50-51 0.15372163101321235 1101 52-53 0.2108180655843057 1101 54-55 0.3080330375928142 1101 56-57 0.22835041743424966 1101 58-59 0.1861024681009198 1101 60-61 -0.006956329195354272 1101 62-63 -0.19397036552356184 1101 64-65 -0.2751452349994885 1101 66-67 -0.2993636119629848 1101 68-69 -0.2848719904680479 1101 70-71 -0.31658961521217677 1101 72-73 -0.3675208655053872 1101 74-75 -0.3056450481605246 1101 76-77 -0.38540366644704704 1101 78-79 -0.689699967490192 1101 80-81 -0.7037524283815877 1101 82-83 -0.8748825434189023 1101 84-85 -0.9172290227082733 1101 86-87 -0.6975059041937222 1101 88-89 -0.640303730046746 1101 90-91 -0.9662590018569261 1101 92-93 -1.0651028922384036 1101 94-95 -1.265197938587015 1101 96-97 -1.2634310408951634 1101 98-99 -1.2668535865474944 1101 100-101 -1.4420767070574367 1102 1 0.1850177717038477 1102 2 0.18531441536351423 1102 3 0.24742178232798295 1102 4 0.15870105926730815 1102 5 0.1649423841721429 1102 6 0.1935915670449333 1102 7 0.2527272296026979 1102 8 0.1725022244279586 1102 9 0.22257013130413128 1102 10-11 0.21564121656062696 1102 12-13 0.18811042117871324 1102 14-15 0.295502244775534 1102 16-17 0.2151726221522594 1102 18-19 0.24695961710640546 1102 20-21 0.18690698647154846 1102 22-23 0.38306546255489593 1102 24-25 0.3029402891755666 1102 26-27 0.2737285917163277 1102 28-29 0.18644144904258297 1102 30-31 0.1921819230860251 1102 32-33 0.29076767185173935 1102 34-35 0.301523610176055 1102 36-37 0.326541840600143 1102 38-39 0.24421872950510704 1102 40-41 0.23658383400750438 1102 42-43 0.24568249012329346 1102 44-45 0.2045424188593188 1102 46-47 0.17238230223365747 1102 48-49 0.014243454466949856 1102 50-51 -0.053837410676628394 1102 52-53 0.07170663322532533 1102 54-55 0.1071624955266941 1102 56-57 0.17961285215598366 1102 58-59 0.07464432997028325 1102 60-61 0.23092861052605684 1102 62-63 0.06295098923025932 1102 64-65 0.010501826623585231 1102 66-67 -0.12111318338461174 1102 68-69 -0.2619364789948406 1102 70-71 -0.6786248592762796 1102 72-73 -0.4620266345817079 1102 74-75 -0.4658457090982715 1102 76-77 -0.582043354241712 1102 78-79 -0.6530981353858287 1102 80-81 -0.4799240659624324 1102 82-83 -0.5840141132600714 1102 84-85 -0.5470735446884696 1102 86-87 -0.6043246876756356 1102 88-89 -0.6620595479547298 1102 90-91 -0.7100499448483184 1102 92-93 -0.6695002203286542 1102 94-95 -0.7362658002164366 1102 96-97 -0.9942246321984669 1102 98-99 -0.9816740198950065 1102 100-101 -1.0051955677776458 1103 1 0.21072482199406295 1103 2 0.19982948712792847 1103 3 0.23125013274022876 1103 4 0.20948909155660544 1103 5 0.25693748512682646 1103 6 0.19955037216388405 1103 7 0.1747730085261523 1103 8 0.1258660430432812 1103 9 0.18111762286842747 1103 10-11 0.22624528339824934 1103 12-13 0.15983422212386955 1103 14-15 0.27100261190393127 1103 16-17 0.24085332844880014 1103 18-19 0.2775060871612496 1103 20-21 0.21517812297955885 1103 22-23 0.29365919804694585 1103 24-25 0.23587898135846785 1103 26-27 0.3090386370842495 1103 28-29 0.24271746959578167 1103 30-31 0.24231913685525797 1103 32-33 0.2794177231147117 1103 34-35 0.08051925338575217 1103 36-37 0.1579673750363355 1103 38-39 0.11891422668846019 1103 40-41 0.20654886868406663 1103 42-43 0.16542499774148922 1103 44-45 0.257356688641309 1103 46-47 0.20101456708210463 1103 48-49 0.19824059394048987 1103 50-51 0.297587902507523 1103 52-53 0.38229875620246645 1103 54-55 0.2809462280262167 1103 56-57 0.29677134212201395 1103 58-59 0.2960734719398417 1103 60-61 0.1559204167002619 1103 62-63 0.09559344742299913 1103 64-65 -0.01962342078495638 1103 66-67 0.023787031942376302 1103 68-69 -0.14714557342896128 1103 70-71 -0.4317465756521557 1103 72-73 -0.40775172628880796 1103 74-75 -0.49476682036855024 1103 76-77 -0.3880774975575392 1103 78-79 -0.34285560939246196 1103 80-81 -0.3755863820888514 1103 82-83 -0.5398098293704479 1103 84-85 -0.7992193145368383 1103 86-87 -1.0138266005683327 1103 88-89 -0.9806791826863481 1103 90-91 -1.0133364377483751 1103 92-93 -0.8675942089480522 1103 94-95 -0.848893967026978 1103 96-97 -0.9521927434034936 1103 98-99 -1.1727408185128922 1103 100-101 -1.3059212557402606 1104 1 0.11801215344954841 1104 2 0.05292100077395645 1104 3 0.05982265351561722 1104 4 0.13207223381323985 1104 5 0.06372195273250725 1104 6 0.10765445475148994 1104 7 0.12219724269144905 1104 8 0.05154328281734166 1104 9 0.08162689330348627 1104 10-11 0.09998661946505649 1104 12-13 0.14186056486980902 1104 14-15 0.15912761677081022 1104 16-17 -0.011454723531620914 1104 18-19 0.06771785135077835 1104 20-21 0.054561844872885956 1104 22-23 0.23386221954829978 1104 24-25 0.2383497641440897 1104 26-27 0.19300360899084268 1104 28-29 0.14056216688993572 1104 30-31 0.08389667175698179 1104 32-33 0.008132197537570107 1104 34-35 0.06118817875717042 1104 36-37 0.01483249128577313 1104 38-39 -0.026172187882025355 1104 40-41 0.11709789534324955 1104 42-43 0.07641735302168229 1104 44-45 -0.10469634585018639 1104 46-47 -0.2275463439697134 1104 48-49 -0.16569362281729383 1104 50-51 -0.32636695690509754 1104 52-53 -0.33199939707278503 1104 54-55 -0.47141907617331213 1104 56-57 -0.3514684574261935 1104 58-59 -0.4842982551268804 1104 60-61 -0.42048147511835055 1104 62-63 -0.3299676066266315 1104 64-65 -0.6347935598680259 1104 66-67 -0.5702956193786868 1104 68-69 -0.462801657313463 1104 70-71 -0.4368268915782174 1104 72-73 -0.314318139924854 1104 74-75 -0.5475523930217037 1104 76-77 -0.7691240221089188 1104 78-79 -0.6491816168014495 1104 80-81 -0.7956400735208078 1104 82-83 -0.9101939908758574 1104 84-85 -0.9818287627280924 1104 86-87 -0.9817434126515039 1104 88-89 -0.8969736066918621 1104 90-91 -0.9690164950041371 1104 92-93 -1.1270496739776519 1104 94-95 -1.1176456173421059 1104 96-97 -1.3151399566804898 1104 98-99 -1.415953997106545 1104 100-101 -1.803968928521618 1105 1 0.019479616689508816 1105 2 0.09822427027724956 1105 3 0.1629038655251165 1105 4 0.14841674508400615 1105 5 0.14223046807963158 1105 6 0.11431026269772815 1105 7 0.14322516149012188 1105 8 0.18580875649396233 1105 9 0.31796350428749065 1105 10-11 0.17613030785049233 1105 12-13 0.2093580138051081 1105 14-15 0.33141889693781224 1105 16-17 0.39019213231453165 1105 18-19 0.3682816065981527 1105 20-21 0.26333393462227406 1105 22-23 0.3601496891458851 1105 24-25 0.26218076545701763 1105 26-27 0.22083358787443785 1105 28-29 0.2340061810369889 1105 30-31 0.15294420971241607 1105 32-33 0.1588987858024069 1105 34-35 0.11606244866516136 1105 36-37 0.1208029343295749 1105 38-39 0.10952016239651385 1105 40-41 0.1572491995249976 1105 42-43 0.23177393777379507 1105 44-45 0.1684504759372416 1105 46-47 0.3303068950142958 1105 48-49 0.280694784129615 1105 50-51 0.24452117089389702 1105 52-53 0.2295918696042918 1105 54-55 0.23442137609973912 1105 56-57 0.2490304693903056 1105 58-59 0.36279571821649625 1105 60-61 0.4713319278550401 1105 62-63 0.4676625076904415 1105 64-65 0.3488995771001022 1105 66-67 0.18801888148746926 1105 68-69 0.13284590575192823 1105 70-71 -0.14142395024068577 1105 72-73 -0.31751673764064847 1105 74-75 -0.28420822100247634 1105 76-77 -0.31711392336535127 1105 78-79 -0.28392040647484507 1105 80-81 -0.25434546494896537 1105 82-83 -0.3738998364202466 1105 84-85 -0.4135849437174528 1105 86-87 -0.43660008555559315 1105 88-89 -0.39213760275440634 1105 90-91 -0.47925489600746474 1105 92-93 -0.8020668202044128 1105 94-95 -0.9467345499953339 1105 96-97 -1.0108472727600741 1105 98-99 -1.2260334073431274 1105 100-101 -1.574339547468682 1106 1 0.245275013064564 1106 2 0.2780300521386536 1106 3 0.3003152591931837 1106 4 0.21763813727710613 1106 5 0.25023583868619426 1106 6 0.2998282789570439 1106 7 0.3628322135563735 1106 8 0.2127268612511557 1106 9 0.4369053039178823 1106 10-11 0.37669567481302835 1106 12-13 0.4586254118807105 1106 14-15 0.4698893369110948 1106 16-17 -0.08208666637015227 1106 18-19 0.36929618705747913 1106 20-21 0.5494629766761179 1106 22-23 0.4550620372028362 1106 24-25 0.3872810847434778 1106 26-27 0.31513154808509825 1106 28-29 0.24129153029993944 1106 30-31 0.19134209443959094 1106 32-33 0.3111671946626089 1106 34-35 0.1867457911085708 1106 36-37 0.19040011714710658 1106 38-39 0.14248399539581413 1106 40-41 0.10481474868903717 1106 42-43 0.0016040782424866507 1106 44-45 0.05460218472716605 1106 46-47 0.004188958576598623 1106 48-49 0.13526435474137344 1106 50-51 -0.003963194480881782 1106 52-53 0.1476798165402684 1106 54-55 0.07320936728465455 1106 56-57 -0.28772868623581616 1106 58-59 -0.1591037108854252 1106 60-61 0.20070357122594373 1106 62-63 0.11389440683756646 1106 64-65 -0.21204954095779982 1106 66-67 -0.3516956260912458 1106 68-69 -0.30621636378484496 1106 70-71 -0.41391716106519283 1106 72-73 -0.17374727027641157 1106 74-75 -0.016553111117609376 1106 76-77 -0.618326647972097 1106 78-79 -0.5646788419711726 1106 80-81 -0.3946292438083816 1106 82-83 -0.4107506350702188 1106 84-85 -0.5351860178387646 1106 86-87 -0.7138424656279518 1106 88-89 -0.8012212158972076 1106 90-91 -1.0145634889779593 1106 92-93 -0.9885072316327879 1106 94-95 -1.1930596105122646 1106 96-97 -1.3099023193082253 1106 98-99 -1.6636239658341516 1106 100-101 -1.5921052797018653 1107 1 -0.12837147325818776 1107 2 -0.12165733250739663 1107 3 0.04179127835661234 1107 4 0.08260377778125161 1107 5 0.019941981564784328 1107 6 0.013843583209542487 1107 7 0.02898484826520331 1107 8 0.02298973221143541 1107 9 0.06918362599692784 1107 10-11 0.09472242807834164 1107 12-13 0.082011112219746 1107 14-15 0.0957838934831301 1107 16-17 0.11556042446466819 1107 18-19 0.11834942411472582 1107 20-21 0.059728535813725614 1107 22-23 0.17668668634652107 1107 24-25 0.2509929527841308 1107 26-27 0.19203859681388735 1107 28-29 0.19101032072504864 1107 30-31 0.0517512743684847 1107 32-33 0.15685565326828055 1107 34-35 0.20274421623004457 1107 36-37 0.1939358899030168 1107 38-39 0.2107245243242133 1107 40-41 0.2297505766509289 1107 42-43 0.17232350006143804 1107 44-45 0.16571061858849134 1107 46-47 0.24370555872726385 1107 48-49 0.42009821554755433 1107 50-51 0.2586184589055378 1107 52-53 0.2479401435668791 1107 54-55 0.3288324113897758 1107 56-57 0.19523826819695955 1107 58-59 0.1891070448399006 1107 60-61 0.265782377439173 1107 62-63 0.23342585097233126 1107 64-65 0.20904130828987277 1107 66-67 0.12322777724616074 1107 68-69 0.009498753672112059 1107 70-71 0.010599475146396031 1107 72-73 0.050481140873099406 1107 74-75 -0.1807632098786236 1107 76-77 -0.3621676075333866 1107 78-79 -0.28406197739564476 1107 80-81 -0.453752262015211 1107 82-83 -0.4065375727576104 1107 84-85 -0.4942996602075844 1107 86-87 -0.535312413444526 1107 88-89 -0.316769043489316 1107 90-91 -0.417724523257462 1107 92-93 -0.3685124047350179 1107 94-95 -0.5255122379433423 1107 96-97 -0.715374366525733 1107 98-99 -0.9607826279610769 1107 100-101 -1.0801635436507198 1108 1 0.047256003302848626 1108 2 0.04391251509674987 1108 3 -0.0014945075279442221 1108 4 0.05316510728599155 1108 5 -0.024819265651494504 1108 6 0.048406629850660465 1108 7 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-0.7975364723144409 2316 52-53 -1.0323488591115506 2316 54-55 -0.9490695919558263 2316 56-57 -0.8131190202383038 2316 58-59 -0.6523874115025947 2316 60-61 -0.7035710450072159 2316 62-63 -0.5100606691317893 2316 64-65 -0.4895569174452099 2316 66-67 -0.4402912250498723 2316 68-69 -0.6134576601950954 2316 70-71 -0.5437155664883377 2316 72-73 -0.3910586328298642 2316 74-75 -0.18496094415310793 2316 76-77 -0.24450255365489326 2316 78-79 -0.16573129509623286 2316 80-81 -0.1828734994926684 2316 82-83 -0.17572174388812556 2316 84-85 -0.16098423926559846 2316 86-87 0.08639373529949523 2316 88-89 0.025280241752827948 2316 90-91 0.0570964933572391 2316 92-93 -0.016369437488211958 2316 94-95 0.009740417029323822 2316 96-97 0.1913998307002629 2316 98-99 0.11163661605129604 2316 100-101 0.22518515182445675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8092.0 3 2412.0 4 2157.0 5 2396.0 6 2872.0 7 3637.0 8 4591.0 9 5849.0 10 7408.0 11 9433.0 12 12090.0 13 15092.0 14 18274.0 15 22924.0 16 27009.0 17 34041.0 18 33559.0 19 28033.0 20 28314.0 21 29570.0 22 32324.0 23 35319.0 24 39318.0 25 43895.0 26 50022.0 27 57535.0 28 66016.0 29 76477.0 30 89317.0 31 107004.0 32 129742.0 33 160389.0 34 207124.0 35 271998.0 36 337014.0 37 352527.0 38 181513.0 39 11655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0537750891107 18.55346740406223 14.689336402975009 22.703421103852058 2 15.459227926387772 19.737325900040226 40.65159424024537 24.15185193332663 3 19.267090397479635 24.06489026881898 29.800355782887504 26.86766355081388 4 12.598026805132069 16.716253382878115 37.05826019816865 33.62745961382117 5 13.43184649632995 37.42468223643552 34.25863167041887 14.884839596815658 6 32.01139775538336 36.154197798185926 17.25277587194481 14.581628574485897 7 28.38568304347604 33.40978439934199 20.139310831393544 18.06522172578843 8 26.609442228712044 34.50985646218759 19.57521325578554 19.305488053314832 9 26.4827811763189 16.79490797994577 20.245905699197532 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22.09909263073423 26.587640773101096 29.677486796596252 21.63577979956842 36-37 22.23565499606841 25.994107131840156 29.737037242250874 22.033200629840564 38-39 21.653853005640393 26.432681091373126 29.65961026541101 22.25385563757547 40-41 22.223346221568164 26.286008028367213 29.68955880122916 21.80108694883546 42-43 21.816338180448135 26.301022683208643 29.478742324768607 22.403896811574615 44-45 21.81460395018765 26.701905571779548 29.59125357675214 21.89223690128066 46-47 22.332172786479152 26.971636591048455 28.72251328644792 21.973677336024473 48-49 22.031653718858287 27.036598998688856 28.64460053215767 22.287146750295182 50-51 22.28373361240321 27.285071215065688 28.550627966148635 21.880567206382466 52-53 22.61579915970941 26.899293945399176 28.533650504150792 21.95125639074062 54-55 21.937306325050425 27.29551647340664 28.625994755394814 22.141182446148125 56-57 22.1187619033967 27.444061031006218 28.387987257486856 22.049189808110224 58-59 22.1001863960356 26.96529969693458 28.336047107718837 22.59846679931098 60-61 21.693896640592428 27.164734321432505 28.779286877314785 22.362082160660282 62-63 21.8723324351918 27.11966442844926 28.91654983232029 22.09145330403865 64-65 21.667760572491783 27.202059920900982 28.693896084052817 22.43628342255441 66-67 21.54615699594357 27.199688133050138 28.71862550729932 22.535529363706974 68-69 22.244042361933978 26.836617551380897 28.74790201877654 22.17143806790859 70-71 21.98146872183913 27.00621297103049 28.270943046979895 22.741375260150484 72-73 21.990770611694945 27.17374289112209 28.315366568166173 22.520119929016793 74-75 22.00729494075512 27.538715584510463 28.14343914899166 22.31055032574275 76-77 22.15930143262391 27.137149671213784 28.201588784844866 22.501960111317437 78-79 22.160853889041395 27.427757048947658 28.06802067097004 22.343368391040904 80-81 22.451039012680226 27.368178180979168 27.734908175137335 22.44587463120327 82-83 22.335681631553463 27.53721651456203 27.789476288523762 22.33762556536075 84-85 22.31107184056157 27.58785558255406 27.789882924674174 22.311189652210196 86-87 22.607047777555675 27.468648421297353 27.44567447767659 22.47862932347038 88-89 22.427163334229107 27.534216221058237 27.317639210398525 22.720981234314127 90-91 22.322115175809042 27.612923943199174 27.369136398590772 22.695824482401015 92-93 22.520072685449964 27.14195254620102 27.564123848179435 22.773850920169583 94-95 22.3221131980578 26.962076420820395 27.748956062792416 22.966854318329386 96-97 22.70707204050831 26.529969191698044 27.711697312902377 23.051261454891268 98-99 22.39846743596049 26.818908222937548 27.277023560441837 23.505600780660117 100-101 23.128367552611515 25.685029318875475 27.63540138976443 23.551201738748585 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 977.0 1 678.0 2 557.5 3 745.5 4 630.5 5 593.0 6 1121.5 7 2359.5 8 3832.0 9 4461.0 10 3787.0 11 3013.0 12 2906.0 13 3012.0 14 3198.5 15 3406.0 16 3679.0 17 4013.5 18 4380.0 19 4884.0 20 5565.5 21 6516.5 22 7842.0 23 9639.0 24 11877.5 25 14984.0 26 18893.0 27 23187.0 28 26670.0 29 30032.5 30 34027.5 31 39694.0 32 47976.5 33 51739.5 34 51966.0 35 54425.5 36 58092.0 37 61771.5 38 64733.0 39 66877.0 40 70113.5 41 74835.5 42 77416.5 43 79154.0 44 81693.5 45 85482.0 46 87583.5 47 89612.5 48 91298.5 49 89089.0 50 86536.0 51 84271.5 52 82564.5 53 83239.0 54 85103.5 55 81282.5 56 74336.0 57 68427.0 58 64058.0 59 57715.5 60 46916.0 61 38758.0 62 33110.0 63 25210.0 64 17608.5 65 13123.5 66 9775.0 67 7377.0 68 5758.5 69 4575.0 70 3587.0 71 2518.5 72 1789.0 73 1356.5 74 1034.0 75 784.5 76 557.5 77 357.5 78 228.5 79 147.5 80 93.5 81 64.5 82 42.0 83 24.0 84 14.5 85 9.0 86 10.0 87 9.0 88 7.0 89 6.0 90 5.0 91 4.0 92 4.5 93 4.5 94 2.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1475102299149333 2 0.05017782108897651 3 0.1499052589340472 4 0.1276040051167243 5 0.19156305875830704 6 0.19780583931632523 7 0.16250860836249903 8 0.0690632138462517 9 0.11935882324764366 10-11 0.10129794867727651 12-13 0.14933594875737255 14-15 0.13547619066315605 16-17 0.13262963977978295 18-19 0.13068613262492826 20-21 0.13245295731115983 22-23 0.19093485442542468 24-25 0.21152817771272373 26-27 0.14466367903156022 28-29 0.12328510032815823 30-31 0.09668457310767187 32-33 0.1217145894959524 34-35 0.09160004428840547 36-37 0.08523947541797183 38-39 0.050511554640820244 40-41 0.057637747541954235 42-43 0.06199591510132543 44-45 0.03875235478467903 46-47 0.051159390359105154 48-49 0.04240379246955761 50-51 0.05272990119131099 52-53 0.026698684147499235 54-55 0.05019745247437908 56-57 0.026011585658409185 58-59 0.05053118602622282 60-61 0.037005161483850045 62-63 0.0516305436087669 64-65 0.040008763450443706 66-67 0.03865419785766617 68-69 0.06431241857882905 70-71 0.05104160204668971 72-73 0.049844087537132764 74-75 0.04193263921989586 76-77 0.020828899912129918 78-79 0.022340516588128037 80-81 0.025697483491968015 82-83 0.02194788888007658 84-85 0.019808067871196124 86-87 0.022733144296179498 88-89 0.06509767399493196 90-91 0.10673584243378924 92-93 0.07866296130810989 94-95 0.07418700543632326 96-97 0.06621666296287862 98-99 0.05512493021042489 100-101 0.1285266802306452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2546942.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.950617883552944 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82468204484456 40.14963765098463 2 13.430932604200162 14.223523602895108 3 4.659288465454557 7.4013660943059 4 2.0949141577267447 4.43707996258536 5 1.1233050803488516 2.9739849038102886 6 0.6568261687091834 2.086761088514283 7 0.41859765868305937 1.5515503270313615 8 0.2988996056621754 1.2661535043970005 9 0.21042280955506765 1.0027816004460617 >10 1.1344279316291517 11.027333335702842 >50 0.09162967959590888 3.3255444470623994 >100 0.049232307226610456 4.8587935118307115 >500 0.003420743181923884 1.309100748443581 >1k 0.0031976512352744372 3.176122705593272 >5k 7.436398221568458E-5 0.25811091277276377 >10k+ 1.4872796443136916E-4 0.9521556036244393 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13489 0.5296155153906136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10692 0.41979754544862036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6555 0.25736746262773164 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3898 0.15304628059845884 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3762 0.147706543768959 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3500 0.13741969781801078 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3471 0.13628107746466153 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3253 0.12772179342913972 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2901 0.11390129810572835 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2856 0.11213447341949678 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2784 0.10930755392152629 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2669 0.10479233527893451 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2573 0.10102310928164049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.926277080514594E-5 0.0 2 0.0 0.0 0.0 3.926277080514594E-5 0.0 3 0.0 0.0 0.0 7.852554161029188E-5 0.0 4 0.0 0.0 0.0 7.852554161029188E-5 0.0 5 0.0 0.0 0.0 7.852554161029188E-5 0.0 6 0.0 0.0 0.0 7.852554161029188E-5 7.852554161029188E-5 7 0.0 0.0 0.0 7.852554161029188E-5 1.177883124154378E-4 8 0.0 0.0 0.0 7.852554161029188E-5 1.177883124154378E-4 9 0.0 0.0 0.0 1.5705108322058376E-4 1.177883124154378E-4 10-11 0.0 0.0 0.0 1.5705108322058376E-4 1.177883124154378E-4 12-13 5.889415620771891E-5 0.0 0.0 1.5705108322058376E-4 4.907846350643242E-4 14-15 7.852554161029188E-5 0.0 0.0 1.5705108322058376E-4 5.496787912720431E-4 16-17 7.852554161029188E-5 0.0 0.0 1.7668246862315672E-4 5.88941562077189E-4 18-19 7.852554161029188E-5 0.0 0.0 2.355766248308756E-4 5.88941562077189E-4 20-21 7.852554161029188E-5 0.0 0.0 2.7483939563602153E-4 6.67467103687481E-4 22-23 1.177883124154378E-4 0.0 0.0 3.5336493724631344E-4 6.87098489090054E-4 24-25 1.177883124154378E-4 0.0 0.0 7.852554161029187E-4 7.067298744926269E-4 26-27 1.177883124154378E-4 0.0 0.0 0.001236777280362097 7.656240307003458E-4 28-29 2.748393956360216E-4 0.0 0.0 0.003651437684878572 7.852554161029187E-4 30-31 3.5336493724631344E-4 0.0 0.0 0.011798462626946353 7.852554161029187E-4 32-33 3.5336493724631344E-4 0.0 0.0 0.027994355584069056 8.245181869080647E-4 34-35 3.729963226488864E-4 0.0 0.0 0.0525728501080904 9.030437285183565E-4 36-37 4.711532496617512E-4 0.0 0.0 0.09556558413972521 9.030437285183565E-4 38-39 5.104160204668971E-4 0.0 0.0 0.16011357934338513 9.030437285183565E-4 40-41 5.104160204668971E-4 0.0 0.0 0.23009946830355776 9.030437285183565E-4 42-43 5.496787912720431E-4 0.0 0.0 0.29984978063889955 9.030437285183565E-4 44-45 5.88941562077189E-4 0.0 0.0 0.38811248940886756 9.030437285183565E-4 46-47 8.245181869080647E-4 0.0 0.0 0.4890570731488978 9.030437285183565E-4 48-49 9.423064993235024E-4 0.0 0.0 0.5952039740206099 9.619378847260754E-4 50-51 9.423064993235024E-4 0.0 0.0 0.7094978998343896 9.815692701286485E-4 52-53 9.423064993235024E-4 0.0 0.0 0.8176864647879692 0.0010993575825440861 54-55 9.423064993235024E-4 0.0 0.0 0.9317840767477233 0.0010993575825440861 56-57 0.0010208320409337943 0.0 0.0 1.0648848697771682 0.0010993575825440861 58-59 0.0010208320409337943 0.0 0.0 1.2021082537411532 0.0010993575825440861 60-61 0.0010208320409337943 0.0 0.0 1.3514442024985258 0.0010993575825440861 62-63 0.0010208320409337943 0.0 0.0 1.4994452170485233 0.0011386203533492322 64-65 0.0010208320409337943 0.0 0.0 1.645973877693328 0.0011386203533492322 66-67 0.0011386203533492322 0.0 0.0 1.8166884051541023 0.001177883124154378 68-69 0.0011386203533492322 0.0 0.0 2.008271095297812 0.001217145894959524 70-71 0.0011386203533492322 0.0 0.0 2.2097480036844184 0.0012956714365698159 72-73 0.001158251738751805 0.0 0.0 2.413816254944164 0.0014330911343878267 74-75 0.001177883124154378 0.0 0.0 2.6145668020708754 0.0015312480614006916 76-77 0.001177883124154378 0.0 0.0 2.831768450164943 0.0015705108322058375 78-79 0.001197514509556951 0.0 0.0 3.073293384772798 0.0015705108322058375 80-81 0.001334934207374962 0.0 0.0 3.3246929062381474 0.0015705108322058375 82-83 0.001334934207374962 0.0 0.0 3.585063970832473 0.0016097736030109833 84-85 0.0013545655927775348 0.0 0.0 3.84934168112191 0.0016490363738161293 86-87 0.0013938283635826806 0.0 0.0 4.121648628040999 0.0016686677592187022 88-89 0.0014134597489852537 0.0 0.0 4.406755238242567 0.0017079305300238482 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5915 0.0 57.64492 1 GTATCAA 10865 0.0 44.09314 1 TATCAAC 13675 0.0 34.499596 2 ATCAACG 13685 0.0 34.336052 3 TCAACGC 13945 0.0 33.764076 4 CAACGCA 14100 0.0 33.325455 5 AACGCAG 14375 0.0 32.748287 6 TGGTATC 2355 0.0 30.292145 2 ACGCAGA 16505 0.0 28.39844 7 CGCAGAG 16630 0.0 28.011265 8 TAAGGTG 1900 0.0 27.784819 5 GTGGTAT 2735 0.0 27.33664 1 GTAAGGT 1815 0.0 26.727709 4 GCAGAGT 18155 0.0 25.238943 9 GGTAAGG 1960 0.0 24.507753 3 GAGTACT 11705 0.0 24.041288 12-13 AGGTAAG 1725 0.0 23.98611 2 GTGTAGC 1670 0.0 23.953314 1 GTACATG 10150 0.0 23.88111 1 TACATGG 9820 0.0 23.876192 2 >>END_MODULE