FastQCFastQC Report
Thu 26 May 2016
SRR937916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546942
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129140.5070394221776546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109560.43016291694117886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68050.2671831553290181No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39800.15626582780448084No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37060.14550782860387085No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36730.144212157167301No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36710.14413363162569073No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35820.14063924502403274No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28100.11032838596246007No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.1101713348792395No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27900.10954313054635716No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA26550.10424265648766246No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA25570.10039490494875816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59600.054.9988061
GTATCAA104550.041.0311931
ATCAACG135050.031.5103323
TATCAAC137150.031.1706582
TCAACGC137250.030.9682124
CAACGCA139450.030.479655
AACGCAG143750.029.6009486
ACGCAGA165700.025.5937797
CGCAGAG166050.025.4540318
TGGTATC24550.025.3465632
GTGTAAG11350.024.694861
TAAGGTG18500.024.6436445
GCAGAGT183250.022.7020769
GTACTAG6150.022.4013061
GTAAGGT18250.022.11884
GTGGTAT31200.022.0782091
GAGTACT119900.022.04375312-13
TAAGGTA9500.021.9955354
AGGTAAG17200.021.5409772
AGAGTAC165050.021.19279510-11
Produced by FastQC (version 0.11.5)