##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937916_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2546942 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.524869039027976 34.0 31.0 34.0 31.0 34.0 2 32.745514424749366 34.0 31.0 34.0 31.0 34.0 3 32.79284805072122 34.0 31.0 34.0 31.0 34.0 4 36.24399063661442 37.0 35.0 37.0 35.0 37.0 5 36.22125317341345 37.0 37.0 37.0 35.0 37.0 6 36.253739975233046 37.0 37.0 37.0 35.0 37.0 7 36.21505201139249 37.0 37.0 37.0 35.0 37.0 8 36.198116407833396 37.0 37.0 37.0 35.0 37.0 9 37.89099908831846 39.0 38.0 39.0 35.0 39.0 10-11 37.87134021897633 39.0 38.0 39.0 35.0 39.0 12-13 37.79522541149347 39.0 38.0 39.0 35.0 39.0 14-15 39.07340332053106 40.0 38.5 41.0 36.0 41.0 16-17 38.962873909182065 40.0 38.0 41.0 36.0 41.0 18-19 39.2230062561299 40.5 39.0 41.0 36.0 41.0 20-21 39.2045949613301 41.0 39.0 41.0 36.0 41.0 22-23 39.11444057226274 40.0 39.0 41.0 35.5 41.0 24-25 39.01387114429775 40.0 38.5 41.0 35.0 41.0 26-27 38.84192042849817 40.0 38.0 41.0 35.0 41.0 28-29 38.72606482597563 40.0 38.0 41.0 35.0 41.0 30-31 38.46937150512261 40.0 38.0 41.0 34.0 41.0 32-33 38.23963089854422 40.0 38.0 41.0 34.0 41.0 34-35 38.07007167811439 40.0 38.0 41.0 34.0 41.0 36-37 38.04208596033989 40.0 38.0 41.0 33.5 41.0 38-39 37.88457098748224 40.0 37.0 41.0 33.0 41.0 40-41 37.69644518799407 40.0 37.0 41.0 33.0 41.0 42-43 37.44541100661107 40.0 37.0 41.0 32.5 41.0 44-45 37.15024350770453 40.0 36.5 41.0 31.5 41.0 46-47 36.73297487732347 40.0 36.0 41.0 31.0 41.0 48-49 36.30301004106101 39.0 35.5 41.0 30.0 41.0 50-51 35.90678861159775 39.0 35.0 40.5 29.0 41.0 52-53 35.38945861350592 38.5 35.0 40.0 27.0 41.0 54-55 35.076868652682315 38.0 34.5 40.0 26.5 41.0 56-57 34.73218373249175 38.0 34.0 40.0 26.0 41.0 58-59 34.29240595192195 38.0 34.0 40.0 24.5 41.0 60-61 33.82329652579446 37.0 33.0 40.0 23.0 41.0 62-63 33.36950016922254 36.5 33.0 39.5 22.0 41.0 64-65 32.88531894326608 36.0 32.0 39.0 21.0 40.0 66-67 32.34188685882914 35.5 31.5 39.0 18.5 40.0 68-69 31.77008192569756 35.0 31.0 38.0 16.5 40.0 70-71 31.166047950836727 35.0 30.5 37.5 10.5 39.5 72-73 30.5955644062566 34.5 29.5 37.0 7.5 39.0 74-75 30.015436354655897 34.0 29.0 36.0 6.5 39.0 76-77 28.67046363835533 32.5 27.0 35.0 2.0 37.0 78-79 29.345833356236618 34.0 29.0 35.0 2.0 37.5 80-81 29.026268364179476 34.0 29.0 35.0 2.0 37.0 82-83 28.463836436008357 34.0 27.5 35.0 2.0 36.5 84-85 27.870398501418563 33.0 26.5 35.0 2.0 36.0 86-87 27.237356013603765 33.0 25.5 35.0 2.0 36.0 88-89 26.484801970362888 32.0 24.5 35.0 2.0 35.0 90-91 25.934522262383673 32.0 23.5 34.0 2.0 35.0 92-93 25.121219485956097 31.5 19.5 34.0 2.0 35.0 94-95 24.300288149474937 31.0 14.0 34.0 2.0 35.0 96-97 23.257334285586403 31.0 2.0 34.0 2.0 35.0 98-99 21.81251104265429 30.0 2.0 34.0 2.0 35.0 100-101 17.447672542209443 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0594871068384748 1101 2 0.03106163562285502 1101 3 0.034001675968482914 1101 4 0.042921091318717686 1101 5 0.04230646424910134 1101 6 0.007036683379496367 1101 7 -0.031882737509640435 1101 8 0.011384500337072723 1101 9 -0.06657506165209526 1101 10-11 0.06334423949931534 1101 12-13 0.12704675677132826 1101 14-15 0.049157346698009974 1101 16-17 0.15467548346448012 1101 18-19 0.07918821088505723 1101 20-21 0.06378777627498522 1101 22-23 0.03196296772940599 1101 24-25 0.1470488854014178 1101 26-27 0.17423900710002727 1101 28-29 0.10088824767050397 1101 30-31 0.1568667737873355 1101 32-33 0.11600929373674518 1101 34-35 0.07476969445603743 1101 36-37 0.23759760978639832 1101 38-39 0.18079364726938252 1101 40-41 0.16227723805680228 1101 42-43 0.20077633359956337 1101 44-45 0.12660625414569182 1101 46-47 0.1539099099733363 1101 48-49 0.15853395390584524 1101 50-51 0.07963204077691444 1101 52-53 0.006285219128542963 1101 54-55 -0.22386242402905765 1101 56-57 -0.12720480867716333 1101 58-59 0.02792420827952924 1101 60-61 -0.07970854316457121 1101 62-63 -0.1043923047067139 1101 64-65 0.04776252369840961 1101 66-67 -0.12780630605357146 1101 68-69 -0.023432715754658773 1101 70-71 -0.17444284674439103 1101 72-73 -0.46354571833980884 1101 74-75 -0.4466874556821381 1101 76-77 -0.572314438921186 1101 78-79 -0.5788356376832695 1101 80-81 -0.4392382000975452 1101 82-83 -0.4523553137866081 1101 84-85 -0.3945092057024162 1101 86-87 -0.7682277319875723 1101 88-89 -0.8592795684256238 1101 90-91 -1.182112085333472 1101 92-93 -1.2911394873837914 1101 94-95 -1.4213858795957037 1101 96-97 -1.2274324489164528 1101 98-99 -1.1191147263842147 1101 100-101 -1.5596857581502874 1102 1 0.13525599477551964 1102 2 0.12165234501549094 1102 3 0.12014766329505733 1102 4 0.03678922303430454 1102 5 0.11841444715950189 1102 6 0.06330562733661083 1102 7 0.07179921916750232 1102 8 0.09179085906188078 1102 9 0.1268770342423764 1102 10-11 0.15126472470257823 1102 12-13 0.17485458708900126 1102 14-15 0.21335823766509066 1102 16-17 0.19488233139823308 1102 18-19 0.1469487067172608 1102 20-21 0.12212738088231134 1102 22-23 0.14058837387495515 1102 24-25 0.15931490243350765 1102 26-27 0.08027460017416388 1102 28-29 0.01435230112456054 1102 30-31 0.0165111999880736 1102 32-33 -0.05015200243891371 1102 34-35 -0.016429997780576855 1102 36-37 0.07263682879712974 1102 38-39 7.417102239060114E-5 1102 40-41 -0.08273524004331989 1102 42-43 -0.03236920854091352 1102 44-45 -0.10484331737509223 1102 46-47 -0.028025846401796173 1102 48-49 -0.018293763043054412 1102 50-51 0.01707675193641478 1102 52-53 -0.08563222413501137 1102 54-55 -0.09641030632145231 1102 56-57 -0.0518315315423763 1102 58-59 -0.020930156378440756 1102 60-61 -0.40091179490689655 1102 62-63 -0.22623745728457578 1102 64-65 -0.10800838691039871 1102 66-67 -0.3355945838774588 1102 68-69 -0.047717864882084626 1102 70-71 -0.13111305308665422 1102 72-73 -0.2844925666899911 1102 74-75 -0.22856431661452703 1102 76-77 -0.3640502383276747 1102 78-79 -0.4659413034446871 1102 80-81 -0.36930442276792874 1102 82-83 -0.5554513897042241 1102 84-85 -0.6122969183680134 1102 86-87 -0.7261492186116669 1102 88-89 -0.8775670991499105 1102 90-91 -1.075169462780881 1102 92-93 -0.9816062585551677 1102 94-95 -0.8798477368644804 1102 96-97 -0.9120519139594307 1102 98-99 -1.117322778005974 1102 100-101 -1.3999868982827728 1103 1 0.06529703515514029 1103 2 0.04461631993225268 1103 3 0.060227546533973 1103 4 0.04797801403062607 1103 5 0.017871704639134123 1103 6 0.00636866316969531 1103 7 -0.014984646083249231 1103 8 0.015651467272505215 1103 9 0.10957943673959392 1103 10-11 0.0638092282392293 1103 12-13 0.10893572733954926 1103 14-15 0.1294453052204645 1103 16-17 0.034227939095103466 1103 18-19 0.06125619531153603 1103 20-21 0.0802903063893794 1103 22-23 0.08534089408576762 1103 24-25 0.1438858534648091 1103 26-27 0.06899140205457854 1103 28-29 0.08311443717432354 1103 30-31 0.06571118633794981 1103 32-33 0.10722701581700278 1103 34-35 0.06725568256196368 1103 36-37 0.09476672099863492 1103 38-39 0.07593081019630432 1103 40-41 0.21857179704677776 1103 42-43 0.1275669583686323 1103 44-45 0.125224123248735 1103 46-47 0.0062503144026848645 1103 48-49 -0.18562490460611514 1103 50-51 -0.16080389345955837 1103 52-53 -0.3488889717599264 1103 54-55 -0.33999146569717453 1103 56-57 -0.34761598629857815 1103 58-59 -0.26099978327248863 1103 60-61 -0.35286025668016663 1103 62-63 -0.25779774921728205 1103 64-65 -0.09256246278560809 1103 66-67 -0.29131558054667295 1103 68-69 -0.46102212544649745 1103 70-71 -0.6140001447922536 1103 72-73 -0.6227396017250655 1103 74-75 -0.44549697949166145 1103 76-77 -0.32024289560975205 1103 78-79 -0.3737539194613859 1103 80-81 -0.44582369307117986 1103 82-83 -0.4858796211653633 1103 84-85 -0.5398556232860336 1103 86-87 -0.6750612772982088 1103 88-89 -0.8667869681836997 1103 90-91 -1.000903775823275 1103 92-93 -0.9709254097573172 1103 94-95 -0.9854117758318424 1103 96-97 -1.282990532641655 1103 98-99 -1.1916476217607759 1103 100-101 -1.5727836216524622 1104 1 0.10178395404069107 1104 2 0.07013558959569366 1104 3 0.03425061307255817 1104 4 0.07970706998312238 1104 5 0.0033749472207702524 1104 6 -0.06697063312531526 1104 7 0.027045915806134246 1104 8 0.03608617355003929 1104 9 0.07403191438910994 1104 10-11 0.0466956816031896 1104 12-13 0.027247871747519525 1104 14-15 0.028108965865413893 1104 16-17 0.025827790858748756 1104 18-19 -0.007772725642531952 1104 20-21 0.013981264042904229 1104 22-23 -0.023375937677307945 1104 24-25 0.02719353096084376 1104 26-27 -0.05070617526511967 1104 28-29 -0.1363649091982424 1104 30-31 -0.1864614778600071 1104 32-33 -0.2549296389968916 1104 34-35 -0.16587668102908992 1104 36-37 -0.25827948640908005 1104 38-39 -0.09844387522619513 1104 40-41 -0.08899809667470038 1104 42-43 -0.15144496212741387 1104 44-45 -0.23465164562998098 1104 46-47 -0.1415008502861994 1104 48-49 -0.1936815395277378 1104 50-51 -0.2336106644768705 1104 52-53 -0.3277187470197447 1104 54-55 -0.3213816628430308 1104 56-57 -0.21321211020831043 1104 58-59 -0.12156355215687142 1104 60-61 -0.3395357096894571 1104 62-63 -0.2512010901276156 1104 64-65 -0.4261061928623633 1104 66-67 -0.725096354278115 1104 68-69 -0.6507881895165966 1104 70-71 -0.7133949525557632 1104 72-73 -0.7847972730268893 1104 74-75 -0.7619951222898464 1104 76-77 -0.7503015671883695 1104 78-79 -0.7496766801120707 1104 80-81 -0.6835956101409408 1104 82-83 -0.839288455457087 1104 84-85 -0.8124629052727705 1104 86-87 -1.0295550721133324 1104 88-89 -1.0272810433311754 1104 90-91 -1.1337193673988644 1104 92-93 -1.1169714847858607 1104 94-95 -0.9590961074480227 1104 96-97 -1.22922919457854 1104 98-99 -1.24371130321828 1104 100-101 -1.8257064049562644 1105 1 0.07703328388534203 1105 2 0.09171813123080597 1105 3 0.06570918097695966 1105 4 0.04323535942077683 1105 5 -3.481380448917548E-4 1105 6 -0.01628575239092811 1105 7 0.04469300605639148 1105 8 -0.027033811971669763 1105 9 -0.08125429904284687 1105 10-11 0.014959885757811264 1105 12-13 0.04123615421315918 1105 14-15 -0.02400437753861695 1105 16-17 -0.004344726561278378 1105 18-19 0.04570847594901295 1105 20-21 0.029293412186305545 1105 22-23 0.058582191470677 1105 24-25 0.018795302414829962 1105 26-27 0.10256606607895691 1105 28-29 0.057303224664536856 1105 30-31 0.08726684643775684 1105 32-33 0.12947273736662623 1105 34-35 0.1191882709481149 1105 36-37 0.11299801266599019 1105 38-39 0.12409494837234547 1105 40-41 0.14278958862809077 1105 42-43 0.07977265704803926 1105 44-45 -0.009300390065391184 1105 46-47 0.06602684412563775 1105 48-49 0.08589536772703354 1105 50-51 -0.010667477088475152 1105 52-53 -0.03802548249838367 1105 54-55 0.002827523794735498 1105 56-57 -0.054122339443509304 1105 58-59 -0.1268798007026959 1105 60-61 -0.13292910323325913 1105 62-63 -0.14345016085888318 1105 64-65 -0.07300586456304359 1105 66-67 -0.34294043413846254 1105 68-69 -0.23162405054100077 1105 70-71 -0.20284668400745076 1105 72-73 -0.3273641583371685 1105 74-75 -0.4123254584560634 1105 76-77 -0.2714858388727528 1105 78-79 -0.32849990468456625 1105 80-81 -0.41391017244635364 1105 82-83 -0.2430893582134388 1105 84-85 -0.5549648118931181 1105 86-87 -0.6811531772465855 1105 88-89 -0.6972743618122372 1105 90-91 -0.7148925599828786 1105 92-93 -0.7245006150063595 1105 94-95 -0.9440327215039872 1105 96-97 -0.9332029286293775 1105 98-99 -0.8977095461891587 1105 100-101 -1.6366415404632129 1106 1 0.1512630486718649 1106 2 0.14541118241207585 1106 3 0.2061366604359094 1106 4 0.13600955101000523 1106 5 0.12236458222372448 1106 6 0.07496358604404207 1106 7 0.1350562811720053 1106 8 0.1770286310903586 1106 9 0.06541900496471698 1106 10-11 0.15509616815281646 1106 12-13 0.046013521314506534 1106 14-15 0.09110685214808711 1106 16-17 0.2723488799008038 1106 18-19 0.19425693721384363 1106 20-21 0.255258537948734 1106 22-23 0.2851074784380643 1106 24-25 0.27090309196340456 1106 26-27 0.13457713699005325 1106 28-29 0.25324305912144496 1106 30-31 0.2577038914762326 1106 32-33 0.3825072602155686 1106 34-35 0.33975513456594797 1106 36-37 0.2785871959693935 1106 38-39 0.32814003890914734 1106 40-41 0.3792074417736728 1106 42-43 0.33325400742155864 1106 44-45 0.2154392136029344 1106 46-47 0.24232795815775177 1106 48-49 0.2766034158282338 1106 50-51 0.17733954200797086 1106 52-53 0.22926522435673746 1106 54-55 0.04236471025629385 1106 56-57 0.11377961011270088 1106 58-59 0.23736868312040826 1106 60-61 0.020563200921806413 1106 62-63 0.1550685842064965 1106 64-65 0.056300273025129854 1106 66-67 0.06024820082514282 1106 68-69 -0.11032881467680511 1106 70-71 -0.37934518301402065 1106 72-73 -0.44256215064999793 1106 74-75 -0.2128115460689628 1106 76-77 -0.26382957938309204 1106 78-79 -0.20018882596483678 1106 80-81 -0.08792021088219926 1106 82-83 0.04267365401141632 1106 84-85 -0.19483809507765315 1106 86-87 -0.1854354551125681 1106 88-89 -0.4161911087343313 1106 90-91 -0.7277480698434502 1106 92-93 -0.5707729672279207 1106 94-95 -0.6610602122420808 1106 96-97 -1.0466521117592507 1106 98-99 -1.1008452559508228 1106 100-101 -1.4493649542254978 1107 1 0.01013701708381376 1107 2 -0.0015275409135000473 1107 3 0.004544013715516826 1107 4 0.03938563947595952 1107 5 -0.0436824923509036 1107 6 0.008588881661594883 1107 7 0.03858972635973856 1107 8 0.00695715835601618 1107 9 0.015338916949858117 1107 10-11 0.0448418862469353 1107 12-13 -0.009451280937270212 1107 14-15 0.050664043776450285 1107 16-17 -0.057674225311181715 1107 18-19 0.02493405630075074 1107 20-21 0.053222014142299656 1107 22-23 -0.015264702991615309 1107 24-25 0.020748357853648258 1107 26-27 0.03262112737293421 1107 28-29 0.10945786284844417 1107 30-31 -0.00498233856487218 1107 32-33 -0.07682963200229409 1107 34-35 0.02754202373501613 1107 36-37 0.07649215513205121 1107 38-39 -0.03132647590827986 1107 40-41 -0.069790130343236 1107 42-43 -0.04851552792371194 1107 44-45 -0.1253774153309024 1107 46-47 -0.04819254509526871 1107 48-49 0.017220857378411836 1107 50-51 0.04180606666020026 1107 52-53 0.10017058936860224 1107 54-55 0.1386657126841797 1107 56-57 0.03958247361164524 1107 58-59 0.0533398330767767 1107 60-61 -0.10907054475170952 1107 62-63 -0.2751678049288131 1107 64-65 -0.3601667473007666 1107 66-67 -0.2150839365971038 1107 68-69 -0.1386440093696848 1107 70-71 -0.2301323272321092 1107 72-73 -0.43560714746024587 1107 74-75 -0.41165618712188845 1107 76-77 -0.49241349346107555 1107 78-79 -0.5069973890553747 1107 80-81 -0.2655785723922186 1107 82-83 -0.1755951991269029 1107 84-85 -0.5025583436750445 1107 86-87 -0.4508115016167267 1107 88-89 -0.7747372506425556 1107 90-91 -1.0740335005124066 1107 92-93 -0.9996098650188294 1107 94-95 -1.1761913723479545 1107 96-97 -1.1433486368638768 1107 98-99 -1.3050941169176333 1107 100-101 -1.89582777181562 1108 1 -0.052671733607553506 1108 2 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2.7292603155851296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 6.0 4 25.0 5 34.0 6 100.0 7 270.0 8 603.0 9 1223.0 10 2132.0 11 3565.0 12 5550.0 13 8222.0 14 11216.0 15 15483.0 16 20515.0 17 28984.0 18 29575.0 19 22216.0 20 20735.0 21 20953.0 22 22156.0 23 23985.0 24 27254.0 25 30827.0 26 35709.0 27 41797.0 28 50182.0 29 60203.0 30 72729.0 31 88459.0 32 110634.0 33 142651.0 34 193866.0 35 277001.0 36 393396.0 37 489820.0 38 279003.0 39 15857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13515610520728 19.081069789503776 15.16430391119013 22.619470194098817 2 15.671794469041753 20.06797365088403 40.163710081187475 24.09652179888674 3 19.32957397174157 23.959706428963813 30.092680396543148 26.61803920275147 4 12.844236613347782 17.017911690106562 36.78363055274172 33.35422114380393 5 13.371073093890807 37.561623547851596 34.32358978466506 14.743713573592531 6 32.0963049342144 36.40391690322783 17.009277909309713 14.490500253248054 7 28.456831761382865 33.406492962933584 20.019340840898614 18.11733443478493 8 26.685452593737903 34.71606342036843 19.408333601628932 19.190150384264737 9 26.28465822935897 16.782910643430434 20.20250166670462 36.72992946050597 10-11 25.016961516987823 26.80386911048622 26.727993805905275 21.451175566620677 12-13 26.086092736033372 24.04422111657652 27.447281587858 22.42240455953211 14-15 22.50832567054923 25.496595525143483 26.185009317055513 25.81006948725177 16-17 21.684157003709437 28.73257796541458 27.155964203295596 22.427300827580385 18-19 21.751064610030383 26.669983062040675 30.010341813830077 21.568610514098868 20-21 22.224141735461586 25.817784621714985 30.525881625887045 21.432192016936387 22-23 21.999931682778797 25.83095728132011 30.459606068767958 21.709504967133135 24-25 21.783147185559926 25.99659619613433 30.56925272389709 21.651003894408657 26-27 21.24716884993702 26.463789259350527 30.24906266475158 22.039979225960874 28-29 21.518511930635086 26.85593437630435 30.056880047338524 21.56867364572204 30-31 21.580035188414286 26.398338974918538 29.940424703193457 22.081201133473716 32-33 21.225002375397633 26.316421810940337 30.072534042785428 22.386041770876606 34-35 22.086087468864125 26.56794546512242 29.76553387412458 21.58043319188888 36-37 22.19439342971763 26.010763971027778 29.798511733278104 21.99633086597649 38-39 21.658450051671227 26.419918236958907 29.71833684613911 22.20329486523076 40-41 22.22362916911083 26.27699080367162 29.69053188874571 21.80884813847184 42-43 21.821814452451687 26.235834528922258 29.591336318968203 22.351014699657853 44-45 21.86221751417975 26.660383314578816 29.649889946453435 21.827509224788 46-47 22.310262511091743 26.961530306956583 28.791883593645707 21.936323588305967 48-49 22.03507319889569 27.009806510228685 28.6819173118387 22.273202979036924 50-51 22.304871175151888 27.23716104936707 28.60971957706963 21.84824819841141 52-53 22.68489978239 26.91335415457613 28.51768797820602 21.88405808482785 54-55 22.00292958799454 27.259539468453138 28.63985405853561 22.097676885016714 56-57 22.13989949930288 27.358686724919345 28.424756714919543 22.07665706085823 58-59 22.06240648452503 26.989734406233186 28.316741086330737 22.631118022911046 60-61 21.659679628907607 27.260814202906403 28.732823450375538 22.34668271781045 62-63 21.9008485155984 27.211718916592414 28.81452078130823 22.072911786500956 64-65 21.59903991002298 27.28056713168407 28.596803051176 22.52358990711695 66-67 21.516180688411602 27.334293823646753 28.57238782761123 22.577137660330415 68-69 22.26966187107718 26.89068121825856 28.628632383130824 22.211024527533436 70-71 21.932412888014667 27.123593325953333 28.16603515148707 22.777958634544934 72-73 21.933417791549193 27.27350542627349 28.224632154236172 22.56844462794114 74-75 21.968183321120616 27.666161664950607 27.917209563146027 22.448445450782753 76-77 21.96806664080037 27.266743846674636 28.249355567877828 22.515833944647166 78-79 22.055890771193205 27.59903828632111 28.047337928781037 22.297733013704654 80-81 22.220453014478032 27.547664060041193 27.799877603983113 22.432005321497662 82-83 22.172943515722807 27.722002111102928 27.817600706974005 22.28745366620026 84-85 22.083680636347484 27.871265694905468 27.808039365262633 22.23701430348442 86-87 22.315448070250472 27.77613149911052 27.478446350200198 22.42997408043881 88-89 22.064999243044106 27.938643177667466 27.271874012692905 22.724483566595527 90-91 21.98387240881961 27.92278471442892 27.443551958118928 22.649790918632544 92-93 22.081220417127703 27.568345578020235 27.577692317510312 22.772741687341746 94-95 21.841033955883386 27.405599455581214 27.78599362514819 22.96737296338721 96-97 22.17817139910864 27.00622625835271 27.77805374990158 23.03754859263707 98-99 21.87332991184094 27.275197544064806 27.373051702886187 23.478420841208067 100-101 22.20432960710134 26.835211959753792 27.554561841334653 23.405896591810222 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1018.0 1 691.5 2 530.0 3 702.0 4 563.0 5 517.5 6 1072.5 7 2437.0 8 3983.0 9 4686.5 10 3875.0 11 2823.0 12 2704.5 13 2904.5 14 3076.5 15 3268.5 16 3616.5 17 3863.5 18 4133.0 19 4578.5 20 5188.5 21 6130.0 22 7498.0 23 9296.5 24 11533.5 25 14569.5 26 18631.0 27 23327.0 28 27101.5 29 30454.5 30 34341.5 31 40288.5 32 49066.0 33 52211.5 34 51747.5 35 54344.5 36 58317.0 37 62032.5 38 64733.5 39 67358.5 40 71081.0 41 75717.0 42 78056.0 43 79914.5 44 82719.5 45 86638.5 46 88504.5 47 90716.0 48 92395.0 49 89891.5 50 87784.5 51 85094.0 52 82900.0 53 83833.0 54 86220.5 55 81699.5 56 73860.5 57 68194.0 58 63804.5 59 57145.0 60 45748.0 61 37416.5 62 31798.0 63 23668.5 64 16061.5 65 11852.5 66 8730.5 67 6559.5 68 5167.0 69 4122.0 70 3208.0 71 2168.0 72 1543.5 73 1254.5 74 930.5 75 645.0 76 454.5 77 302.5 78 190.5 79 95.5 80 57.0 81 37.0 82 21.0 83 12.5 84 9.0 85 6.5 86 4.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007774028619418895 2 0.014409436885488559 3 0.009148225597599003 4 7.852554161029188E-5 5 0.0010208320409337943 6 0.00125640866576467 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.496787912720431E-4 14-15 0.0 16-17 0.0023361348629061833 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003356966903839978 26-27 0.019415440163144664 28-29 0.0070280359741211225 30-31 0.0031802844352168207 32-33 0.0 34-35 0.009481959149442745 36-37 7.656240307003457E-4 38-39 0.002748393956360216 40-41 0.0021987151650881723 42-43 0.003141021664411675 44-45 0.0 46-47 7.852554161029188E-5 48-49 4.515218642591783E-4 50-51 0.003631806299475999 52-53 0.007224349828146853 54-55 0.0065765141098619445 56-57 0.015410637541019779 58-59 0.022458304900543475 60-61 0.003258809976827113 62-63 0.01943507154854724 64-65 0.02018106419384501 66-67 0.012799663282477575 68-69 0.030742749540429267 70-71 0.016529626508966437 72-73 0.0047311638820200855 74-75 0.01633331265494071 76-77 0.02018106419384501 78-79 0.02567785210656544 80-81 0.011405834918894894 82-83 0.03453160692312585 84-85 0.020985950995350503 86-87 0.031233534175493592 88-89 0.021928257494674003 90-91 0.026875366616122394 92-93 0.02353803109768499 94-95 0.014645013510319434 96-97 0.015253586457799197 98-99 0.011582517387518051 100-101 0.010502791190376537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2546942.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.799937275489405 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8629208994745 37.5223174867774 2 14.59076032738888 14.824194188661084 3 4.957658147864608 7.55546168834523 4 2.2238614439339695 4.518880874448997 5 1.2081675720590224 3.068741843943933 6 0.7181276926981907 2.188850504691601 7 0.4713187831009835 1.6760075232802967 8 0.32109045523125457 1.3049099988404855 9 0.23791965205371943 1.0877673060841686 >10 1.2455615256442394 11.685161001069213 >50 0.10016657723427846 3.4983024203909032 >100 0.054950721334195575 5.189162530031525 >500 0.003632180341857929 1.2723258547840333 >1k 0.003632180341854173 3.4036650092207315 >5k 7.728043280540793E-5 0.2671534895182998 >10k+ 1.5456086561081587E-4 0.9370982799120966 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12914 0.5070394221776546 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10956 0.43016291694117886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6805 0.2671831553290181 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3980 0.15626582780448084 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3706 0.14550782860387085 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3673 0.144212157167301 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3671 0.14413363162569073 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3582 0.14063924502403274 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2810 0.11032838596246007 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2806 0.1101713348792395 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2790 0.10954313054635716 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2655 0.10424265648766246 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2557 0.10039490494875816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.926277080514594E-5 0.0 2 0.0 0.0 0.0 3.926277080514594E-5 0.0 3 0.0 0.0 0.0 3.926277080514594E-5 0.0 4 0.0 0.0 0.0 3.926277080514594E-5 0.0 5 0.0 0.0 0.0 3.926277080514594E-5 0.0 6 0.0 0.0 0.0 3.926277080514594E-5 3.926277080514594E-5 7 3.926277080514594E-5 0.0 0.0 3.926277080514594E-5 3.926277080514594E-5 8 3.926277080514594E-5 0.0 0.0 3.926277080514594E-5 3.926277080514594E-5 9 3.926277080514594E-5 0.0 0.0 3.926277080514594E-5 3.926277080514594E-5 10-11 3.926277080514594E-5 0.0 0.0 3.926277080514594E-5 7.852554161029188E-5 12-13 3.926277080514594E-5 0.0 0.0 3.926277080514594E-5 1.9631385402572968E-4 14-15 1.374196978180108E-4 0.0 0.0 3.926277080514594E-5 2.1594523942830264E-4 16-17 1.5705108322058376E-4 0.0 0.0 5.889415620771891E-5 2.355766248308756E-4 18-19 2.1594523942830264E-4 0.0 0.0 1.1778831241543782E-4 3.141021664411675E-4 20-21 2.7483939563602153E-4 0.0 0.0 1.5705108322058376E-4 3.141021664411675E-4 22-23 2.7483939563602153E-4 0.0 0.0 2.355766248308756E-4 3.141021664411675E-4 24-25 4.318904788566053E-4 0.0 0.0 6.87098489090054E-4 3.141021664411675E-4 26-27 4.711532496617512E-4 0.0 0.0 0.0010993575825440863 3.141021664411675E-4 28-29 4.711532496617512E-4 0.0 0.0 0.003494386601657988 3.141021664411675E-4 30-31 4.711532496617512E-4 0.0 0.0 0.011503991845907759 3.141021664411675E-4 32-33 4.711532496617512E-4 0.0 0.0 0.02771951618843303 3.141021664411675E-4 34-35 5.3004740586947E-4 0.0 0.0 0.05249432456648012 3.5336493724631344E-4 36-37 5.496787912720431E-4 0.0 0.0 0.09554595275432264 3.5336493724631344E-4 38-39 5.88941562077189E-4 0.0 0.0 0.16044731289522884 3.729963226488864E-4 40-41 5.88941562077189E-4 0.0 0.0 0.23098288064667355 3.9262770805145936E-4 42-43 6.478357182849079E-4 0.0 0.0 0.3013417659294951 4.122590934540323E-4 44-45 7.067298744926269E-4 0.0 0.0 0.389977471022112 4.907846350643242E-4 46-47 8.245181869080647E-4 0.0 0.0 0.49119689415777823 5.496787912720431E-4 48-49 8.245181869080647E-4 0.0 0.0 0.5974615833419057 5.693101766746161E-4 50-51 8.245181869080647E-4 0.0 0.0 0.7118144033118932 6.28204332882335E-4 52-53 8.245181869080647E-4 0.0 0.0 0.8202974390465114 6.67467103687481E-4 54-55 8.637809577132106E-4 0.0 0.0 0.9350036239537454 6.67467103687481E-4 56-57 9.423064993235024E-4 0.0 0.0 1.0696749278153959 7.459926452977728E-4 58-59 9.423064993235024E-4 0.0 0.0 1.2081939832159507 7.852554161029187E-4 60-61 9.423064993235024E-4 0.0 0.0 1.3587078150974778 7.852554161029187E-4 62-63 9.423064993235024E-4 0.0 0.0 1.5077492930738117 8.245181869080647E-4 64-65 0.0010208320409337943 0.0 0.0 1.6552006288325374 8.245181869080647E-4 66-67 0.0010208320409337943 0.0 0.0 1.8270145138758558 8.441495723106377E-4 68-69 0.001118988967946659 0.0 0.0 2.019971400997745 8.637809577132106E-4 70-71 0.001197514509556951 0.0 0.0 2.223195502685181 8.637809577132106E-4 72-73 0.001236777280362097 0.0 0.0 2.428304217371263 9.226751139209295E-4 74-75 0.0012956714365698159 0.0 0.0 2.630330804549142 0.0010012006555312214 76-77 0.0012956714365698159 0.0 0.0 2.8499274816623226 0.0010208320409337943 78-79 0.0012956714365698159 0.0 0.0 3.0947897517886154 0.0010208320409337943 80-81 0.0016686677592187022 0.0 0.0 3.3493891890745844 0.0010404634263363674 82-83 0.0016882991446212753 0.0 0.0 3.614707362790358 0.0010600948117389403 84-85 0.0017275619154264212 0.0 0.0 3.8846389120757365 0.0010600948117389403 86-87 0.0017668246862315672 0.0 0.0 4.162560435219961 0.0011386203533492322 88-89 0.0017668246862315672 0.0 0.0 4.455068077718299 0.001177883124154378 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5960 0.0 54.998806 1 GTATCAA 10455 0.0 41.031193 1 ATCAACG 13505 0.0 31.510332 3 TATCAAC 13715 0.0 31.170658 2 TCAACGC 13725 0.0 30.968212 4 CAACGCA 13945 0.0 30.47965 5 AACGCAG 14375 0.0 29.600948 6 ACGCAGA 16570 0.0 25.593779 7 CGCAGAG 16605 0.0 25.454031 8 TGGTATC 2455 0.0 25.346563 2 GTGTAAG 1135 0.0 24.69486 1 TAAGGTG 1850 0.0 24.643644 5 GCAGAGT 18325 0.0 22.702076 9 GTACTAG 615 0.0 22.401306 1 GTAAGGT 1825 0.0 22.1188 4 GTGGTAT 3120 0.0 22.078209 1 GAGTACT 11990 0.0 22.043753 12-13 TAAGGTA 950 0.0 21.995535 4 AGGTAAG 1720 0.0 21.540977 2 AGAGTAC 16505 0.0 21.192795 10-11 >>END_MODULE