FastQCFastQC Report
Thu 26 May 2016
SRR937915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482438
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.3059895151459976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.2520103221107637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.14872476170603252No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29740.11980158215431766No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29400.11843196083849829No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25920.10441348384128829No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25730.10364810722362451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58100.037.099411
GTATCAA82150.030.8822421
ATCAACG95800.025.8471223
TCAACGC97000.025.5763634
TATCAAC98550.025.4152262
CAACGCA98500.025.1386245
GTACATG68150.025.1207921
TACATGG65750.024.793532
AACGCAG101250.024.5447776
ACATGGG69000.022.3858853
GAGTACT71500.022.05823112-13
ACGCAGA115000.021.6026737
TAAGGTG11750.021.437695
CGCAGAG116250.021.3703868
GTACTTT76150.020.64972314-15
GTGTAGC17900.019.7143941
GCAGAGT125300.019.2578379
AGAGTAC98850.019.12595710-11
TAGCCCT16250.018.7183074
GTAAGGT11450.018.6787174
Produced by FastQC (version 0.11.5)